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The map label for this gene is benR

Identifier: 50084611

GI number: 50084611

Start: 1433015

End: 1433929

Strand: Reverse

Name: benR

Synonym: ACIAD1435

Alternate gene names: 50084611

Gene position: 1433929-1433015 (Counterclockwise)

Preceding gene: 50084620

Following gene: 50084608

Centisome position: 39.85

GC content: 43.72

Gene sequence:

>915_bases
ATGGAACTTAGACATCTCCGCTATTTTGTGGCTGTGGTTGAGGAGCAAAGCTTCACCAAAGCCGCAGACAAATTATGCAT
TGCACAGCCGCCCTTAAGCCGACAAATTCAAAATCTTGAAGAAGAATTGGGGATTCAGCTTTTAGAGCGCGGCAGCAGAC
CGGTCAAGACAACACCTGAGGGTCACTTTTTTTATCAGTACGCCATCAAACTATTGTCGAATGTCGATCAAATGGTTTCC
ATGACCAAGCGCATTGCCTCGGTTGAAAAAACCATTCGTATCGGATTTGTGGGTTCGCTCCTATTTGGTTTACTGCCACG
GATTATTCATTTGTATCGTCAGGCACACCCCAATTTACGCATTGAACTTTACGAAATGGGCACCAAGGCACAAACCGAAG
CATTAAAAGAAGGCCGTATCGATGCCGGATTTGGTCGACTTAAAATCAGTGATCCCGCCATTAAACGTACCTTATTGCGT
AATGAACGTTTGATGGTAGCCGTACATGCCAGCCATCCGCTTAATCAAATGAAAGATAAAGGCGTGCACTTAAATGATTT
AATTGATGAAAAAATTTTGCTTTATCCAAGCTCGCCTAAGCCTAATTTTTCTACCCATGTAATGAATATCTTTTCTGATC
ATGGCTTAGAACCCACCAAAATTAATGAAGTCCGTGAAGTTCAACTGGCGCTTGGATTGGTGGCAGCGGGTGAAGGAATT
TCATTGGTACCGGCCAGTACGCAAAGTATCCAGCTTTTTAATCTGAGCTATGTGCCGCTTCTAGATCCCGATGCCATCAC
GCCCATCTATATTGCCGTGCGCAATATGGAGGAAAGCACTTATATTTATTCGTTGTACGAAACGATTCGCCAGATATATG
CCTATGAAGGTTTTACTGAGCCGCCAAACTGGTAA

Upstream 100 bases:

>100_bases
CCTTCTTCTTGTCTTTCTTGCTTGTTTATACCTTAAAAGAATGTTTTAATAAGTTCAAACACATTATTTGTATAATAAAA
TACCTATGGAGTATTTTTAA

Downstream 100 bases:

>100_bases
CAAAAAACCCATCAGACTGTGAGGTGCTGATGGGTTTTTATAATTTTTTAGATTAATGCGAGACTGCTTCGTTCACTTTC
ACCGTAGTGGTGTCGTTTTG

Product: benzoate degradation transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MELRHLRYFVAVVEEQSFTKAADKLCIAQPPLSRQIQNLEEELGIQLLERGSRPVKTTPEGHFFYQYAIKLLSNVDQMVS
MTKRIASVEKTIRIGFVGSLLFGLLPRIIHLYRQAHPNLRIELYEMGTKAQTEALKEGRIDAGFGRLKISDPAIKRTLLR
NERLMVAVHASHPLNQMKDKGVHLNDLIDEKILLYPSSPKPNFSTHVMNIFSDHGLEPTKINEVREVQLALGLVAAGEGI
SLVPASTQSIQLFNLSYVPLLDPDAITPIYIAVRNMEESTYIYSLYETIRQIYAYEGFTEPPNW

Sequences:

>Translated_304_residues
MELRHLRYFVAVVEEQSFTKAADKLCIAQPPLSRQIQNLEEELGIQLLERGSRPVKTTPEGHFFYQYAIKLLSNVDQMVS
MTKRIASVEKTIRIGFVGSLLFGLLPRIIHLYRQAHPNLRIELYEMGTKAQTEALKEGRIDAGFGRLKISDPAIKRTLLR
NERLMVAVHASHPLNQMKDKGVHLNDLIDEKILLYPSSPKPNFSTHVMNIFSDHGLEPTKINEVREVQLALGLVAAGEGI
SLVPASTQSIQLFNLSYVPLLDPDAITPIYIAVRNMEESTYIYSLYETIRQIYAYEGFTEPPNW
>Mature_304_residues
MELRHLRYFVAVVEEQSFTKAADKLCIAQPPLSRQIQNLEEELGIQLLERGSRPVKTTPEGHFFYQYAIKLLSNVDQMVS
MTKRIASVEKTIRIGFVGSLLFGLLPRIIHLYRQAHPNLRIELYEMGTKAQTEALKEGRIDAGFGRLKISDPAIKRTLLR
NERLMVAVHASHPLNQMKDKGVHLNDLIDEKILLYPSSPKPNFSTHVMNIFSDHGLEPTKINEVREVQLALGLVAAGEGI
SLVPASTQSIQLFNLSYVPLLDPDAITPIYIAVRNMEESTYIYSLYETIRQIYAYEGFTEPPNW

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787879, Length=273, Percent_Identity=34.0659340659341, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1788887, Length=264, Percent_Identity=31.4393939393939, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI2367136, Length=261, Percent_Identity=30.6513409961686, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI145693105, Length=248, Percent_Identity=29.8387096774194, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=30.4878048780488, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI157672245, Length=278, Percent_Identity=30.2158273381295, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081904, Length=282, Percent_Identity=27.3049645390071, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693193, Length=123, Percent_Identity=39.0243902439024, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787806, Length=249, Percent_Identity=25.7028112449799, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788297, Length=270, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787601, Length=142, Percent_Identity=30.9859154929577, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082132, Length=168, Percent_Identity=29.1666666666667, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788748, Length=255, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=144, Percent_Identity=34.0277777777778, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789506, Length=254, Percent_Identity=22.0472440944882, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788481, Length=142, Percent_Identity=30.2816901408451, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790262, Length=283, Percent_Identity=22.6148409893993, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): BENM_ACIAD (O68014)

Other databases:

- EMBL:   AF009224
- EMBL:   CR543861
- RefSeq:   YP_046121.1
- PDB:   2F6G
- PDB:   2F6P
- PDB:   2F78
- PDB:   2F7A
- PDB:   2F8D
- PDB:   2F97
- PDB:   2H99
- PDB:   2H9B
- PDB:   3K1M
- PDB:   3K1N
- PDB:   3K1P
- PDBsum:   2F6G
- PDBsum:   2F6P
- PDBsum:   2F78
- PDBsum:   2F7A
- PDBsum:   2F8D
- PDBsum:   2F97
- PDBsum:   2H99
- PDBsum:   2H9B
- PDBsum:   3K1M
- PDBsum:   3K1N
- PDBsum:   3K1P
- ProteinModelPortal:   O68014
- SMR:   O68014
- STRING:   O68014
- GeneID:   2880312
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1435
- NMPDR:   fig|62977.3.peg.862
- eggNOG:   COG0583
- HOGENOM:   HBG685425
- OMA:   RIEIEDP
- PhylomeDB:   O68014
- BioCyc:   ASP62977:ACIAD1435-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34533; Mature: 34533

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFVAVVEEQSFTKAADKLCIAQPPLSRQIQNLEEELGIQLLERGSRPVKTTPE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GHFFYQYAIKLLSNVDQMVSMTKRIASVEKTIRIGFVGSLLFGLLPRIIHLYRQAHPNLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IELYEMGTKAQTEALKEGRIDAGFGRLKISDPAIKRTLLRNERLMVAVHASHPLNQMKDK
EEEEECCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEEECCCCHHHHHHC
GVHLNDLIDEKILLYPSSPKPNFSTHVMNIFSDHGLEPTKINEVREVQLALGLVAAGEGI
CCCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
SLVPASTQSIQLFNLSYVPLLDPDAITPIYIAVRNMEESTYIYSLYETIRQIYAYEGFTE
EEEECCCCEEEEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
PPNW
CCCC
>Mature Secondary Structure
MELRHLRYFVAVVEEQSFTKAADKLCIAQPPLSRQIQNLEEELGIQLLERGSRPVKTTPE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GHFFYQYAIKLLSNVDQMVSMTKRIASVEKTIRIGFVGSLLFGLLPRIIHLYRQAHPNLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IELYEMGTKAQTEALKEGRIDAGFGRLKISDPAIKRTLLRNERLMVAVHASHPLNQMKDK
EEEEECCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEEECCCCHHHHHHC
GVHLNDLIDEKILLYPSSPKPNFSTHVMNIFSDHGLEPTKINEVREVQLALGLVAAGEGI
CCCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
SLVPASTQSIQLFNLSYVPLLDPDAITPIYIAVRNMEESTYIYSLYETIRQIYAYEGFTE
EEEECCCCEEEEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
PPNW
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203