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The map label for this gene is astE

Identifier: 50084473

GI number: 50084473

Start: 1291234

End: 1292211

Strand: Direct

Name: astE

Synonym: ACIAD1289

Alternate gene names: 50084473

Gene position: 1291234-1292211 (Clockwise)

Preceding gene: 50084472

Following gene: 50084476

Centisome position: 35.88

GC content: 40.59

Gene sequence:

>978_bases
ATGACACATTTTCTTCATTCAGTATTGAGCCAGCAATTTCCTCACGCATTACAAGGTGAAAACCATAATTTTTCATGGCA
ATGGCAAGATGAGGGCGTTATTTGTTTAACGCCCCATCATGGCTATGAAAAAAGCATCGTTCTATCAGCAGGAATACACG
GTAATGAAACTGCGCCCATTGAGCTGCTTGAACACATCTATCAGGATGTATTTCAAGCAAGCCTCACCCTAAATGTTCGC
TTGCTTTTGGTACTGGGCAATCCTAACGCGATACGCACGGGTCAACGCTATATCGAATATGATATGAATCGGATGTTTTG
TGGTGGATATCAGCAGCTAGAGCCATCTGTGGAGACCCAGCGTGCTGAGAGGCTAGAACAGCACGTTCAGATTTTTTTTG
AACAAGGTTGTTCTACTGCCAAGCGCTATCACTATGACTTACATACAGCTATAAGAGCATCGTTGTTACCTACTTTTGCG
CTATTGCCTCATCTTGAACATGTCCATGATCCGCTACTTTTAAATAGCTTAAATGCAGCCGATTTAGACGCTGTGGTTTT
TCATAATACTGCTGGAAAAACCTTTAGCCATTTTAGTGGGGCGTATTGTCAGGCAGCCAGTGCTACTTTGGAATTGGGAC
AGGCAAAGCCCTTCGGTCAAAATGACTTAAGTCAGTTTGTGGCAATTGATCGTGTATTACGGGTTCTAATTGCAGATGAA
GCTTTGCCCTCAAGACACAAAACCCCAATTCGTCGATTTAAAGTCATAGAATCAATTTTGAAAAAAGATGATGATTTTCA
ATTAAATCTAAAGTCTTCTGCGCCCAATTTTTCTCTTTTTCATCAAGGGGATGTTATTGCTACACAACAAAGTCAAAATT
ACATTGCACATGAGCATGAAGTAAGAATTCTATTTCCTAATGTCAATGTAGCAAAAGGGTTAAGAGCTGGGCTGATTTTG
CAAGAAATGAGCAAATAA

Upstream 100 bases:

>100_bases
ATTATTGATCGAAAGTCGTAGTGCATTGGATGAATTGACCCAAATTTTAAATCTGGGCTCGGTCTATCATTTTCAACAAT
AGAATGGGAAGCATGAGCCA

Downstream 100 bases:

>100_bases
ACGCTATATTGGTATTTTGGGAATGAATGGTTCGCTCTATTAAGAGCACTCGATTCGGTGATACCTCATTCTTTAGATCA
GAATTGAATAAAAAAAGGGA

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTHFLHSVLSQQFPHALQGENHNFSWQWQDEGVICLTPHHGYEKSIVLSAGIHGNETAPIELLEHIYQDVFQASLTLNVR
LLLVLGNPNAIRTGQRYIEYDMNRMFCGGYQQLEPSVETQRAERLEQHVQIFFEQGCSTAKRYHYDLHTAIRASLLPTFA
LLPHLEHVHDPLLLNSLNAADLDAVVFHNTAGKTFSHFSGAYCQAASATLELGQAKPFGQNDLSQFVAIDRVLRVLIADE
ALPSRHKTPIRRFKVIESILKKDDDFQLNLKSSAPNFSLFHQGDVIATQQSQNYIAHEHEVRILFPNVNVAKGLRAGLIL
QEMSK

Sequences:

>Translated_325_residues
MTHFLHSVLSQQFPHALQGENHNFSWQWQDEGVICLTPHHGYEKSIVLSAGIHGNETAPIELLEHIYQDVFQASLTLNVR
LLLVLGNPNAIRTGQRYIEYDMNRMFCGGYQQLEPSVETQRAERLEQHVQIFFEQGCSTAKRYHYDLHTAIRASLLPTFA
LLPHLEHVHDPLLLNSLNAADLDAVVFHNTAGKTFSHFSGAYCQAASATLELGQAKPFGQNDLSQFVAIDRVLRVLIADE
ALPSRHKTPIRRFKVIESILKKDDDFQLNLKSSAPNFSLFHQGDVIATQQSQNYIAHEHEVRILFPNVNVAKGLRAGLIL
QEMSK
>Mature_324_residues
THFLHSVLSQQFPHALQGENHNFSWQWQDEGVICLTPHHGYEKSIVLSAGIHGNETAPIELLEHIYQDVFQASLTLNVRL
LLVLGNPNAIRTGQRYIEYDMNRMFCGGYQQLEPSVETQRAERLEQHVQIFFEQGCSTAKRYHYDLHTAIRASLLPTFAL
LPHLEHVHDPLLLNSLNAADLDAVVFHNTAGKTFSHFSGAYCQAASATLELGQAKPFGQNDLSQFVAIDRVLRVLIADEA
LPSRHKTPIRRFKVIESILKKDDDFQLNLKSSAPNFSLFHQGDVIATQQSQNYIAHEHEVRILFPNVNVAKGLRAGLILQ
EMSK

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily

Homologues:

Organism=Escherichia coli, GI1788040, Length=324, Percent_Identity=41.0493827160494, Blast_Score=251, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASTE_ACIAD (Q6FCP8)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045983.1
- HSSP:   P76215
- ProteinModelPortal:   Q6FCP8
- SMR:   Q6FCP8
- STRING:   Q6FCP8
- GeneID:   2878469
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1289
- NMPDR:   fig|62977.3.peg.412
- eggNOG:   COG2988
- HOGENOM:   HBG298271
- OMA:   RWRWLGH
- PhylomeDB:   Q6FCP8
- ProtClustDB:   PRK05324
- BioCyc:   ASP62977:ACIAD1289-MONOMER
- HAMAP:   MF_00767
- InterPro:   IPR007036
- InterPro:   IPR016681
- PIRSF:   PIRSF017020
- TIGRFAMs:   TIGR03242

Pfam domain/function: PF04952 AstE_AspA

EC number: =3.5.1.96

Molecular weight: Translated: 36749; Mature: 36618

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: ACT_SITE 211-211

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHFLHSVLSQQFPHALQGENHNFSWQWQDEGVICLTPHHGYEKSIVLSAGIHGNETAPI
CHHHHHHHHHHHCCHHHCCCCCCCEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCCHH
ELLEHIYQDVFQASLTLNVRLLLVLGNPNAIRTGQRYIEYDMNRMFCGGYQQLEPSVETQ
HHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCHHHHHHHCCHHCCCCHHHCCCCHHHH
RAERLEQHVQIFFEQGCSTAKRYHYDLHTAIRASLLPTFALLPHLEHVHDPLLLNSLNAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
DLDAVVFHNTAGKTFSHFSGAYCQAASATLELGQAKPFGQNDLSQFVAIDRVLRVLIADE
CCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALPSRHKTPIRRFKVIESILKKDDDFQLNLKSSAPNFSLFHQGDVIATQQSQNYIAHEHE
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCEEEEECCCCCCCCCCE
VRILFPNVNVAKGLRAGLILQEMSK
EEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THFLHSVLSQQFPHALQGENHNFSWQWQDEGVICLTPHHGYEKSIVLSAGIHGNETAPI
HHHHHHHHHHHCCHHHCCCCCCCEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCCHH
ELLEHIYQDVFQASLTLNVRLLLVLGNPNAIRTGQRYIEYDMNRMFCGGYQQLEPSVETQ
HHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCHHHHHHHCCHHCCCCHHHCCCCHHHH
RAERLEQHVQIFFEQGCSTAKRYHYDLHTAIRASLLPTFALLPHLEHVHDPLLLNSLNAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
DLDAVVFHNTAGKTFSHFSGAYCQAASATLELGQAKPFGQNDLSQFVAIDRVLRVLIADE
CCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALPSRHKTPIRRFKVIESILKKDDDFQLNLKSSAPNFSLFHQGDVIATQQSQNYIAHEHE
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCEEEEECCCCCCCCCCE
VRILFPNVNVAKGLRAGLILQEMSK
EEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA