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The map label for this gene is rnt [H]

Identifier: 50084341

GI number: 50084341

Start: 1140263

End: 1140928

Strand: Reverse

Name: rnt [H]

Synonym: ACIAD1149

Alternate gene names: 50084341

Gene position: 1140928-1140263 (Counterclockwise)

Preceding gene: 50084342

Following gene: 50084340

Centisome position: 31.7

GC content: 41.29

Gene sequence:

>666_bases
ATGGAGAAAAGTGTGACACAAGAAAATGCCACCCCTCCTGTGATTGGTCAGCGTTTCCGTGGCTTTTTACCTGTTGTTGT
CGATGTCGAAACAGCAGGTTTTAATGCCCAGACTGATGCACTTCTTGAAATTGCATGCATCCCTATCGTTTATAACGAAC
AGGGTTTATTTGTTCCAGGTGAATCTTATCATGCGCATATCAATCCTTTTGAAGGCGCTAATCTAGATCGTCGTTCGCTG
GATTTTATCGGAATCGATCCATTTAATCCGATGCGAATTGCGATGGCAGAAGATGAACGCAGTGCATTAAAACGGATTTT
TAAATCCATTCATGAGGTACGTAAGCAGCAGCAATGTACACATGCTGTTTTAGTGGGACACAATGCTCACTTTGATTTAG
GTTTTTTACAGGCTGCGATTGCACGTACTAGTACCAAAAACCAGAATCCTTTTCATAGTTTTTCTGTATTTGATACGGTC
ACATTAAGTGCAGTCATGTTTGGCCAAACCGTTTTGGCCAAAGCCTGTATACAAGCTGGCATTGATTTTGATGGCAAAGA
AGCACATTCGGCGTTGTACGATACACAAAAAACTGCTGAACTGTTTTGCTATATTTTAAACAAACTCTCTCCACACCTAC
TTGACACTTTCCTGGCGGATCAATAA

Upstream 100 bases:

>100_bases
ATACCATTACCCTGGTCAAGGAAGATCAGATTATTCCTGAACAGCTTGACTATCTGGATGATGAAAAGATTATTCCACTT
TATGCAGGAAAAACCATTCA

Downstream 100 bases:

>100_bases
GATACCGCCATCCTCAACGTCCCTATCGTCTAGAGGCCTAGGACATCGCCCTTTCACGGCGGTAACCGGGGTTCGAATCC
CCGTAGGGACGCCATCTTCT

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MEKSVTQENATPPVIGQRFRGFLPVVVDVETAGFNAQTDALLEIACIPIVYNEQGLFVPGESYHAHINPFEGANLDRRSL
DFIGIDPFNPMRIAMAEDERSALKRIFKSIHEVRKQQQCTHAVLVGHNAHFDLGFLQAAIARTSTKNQNPFHSFSVFDTV
TLSAVMFGQTVLAKACIQAGIDFDGKEAHSALYDTQKTAELFCYILNKLSPHLLDTFLADQ

Sequences:

>Translated_221_residues
MEKSVTQENATPPVIGQRFRGFLPVVVDVETAGFNAQTDALLEIACIPIVYNEQGLFVPGESYHAHINPFEGANLDRRSL
DFIGIDPFNPMRIAMAEDERSALKRIFKSIHEVRKQQQCTHAVLVGHNAHFDLGFLQAAIARTSTKNQNPFHSFSVFDTV
TLSAVMFGQTVLAKACIQAGIDFDGKEAHSALYDTQKTAELFCYILNKLSPHLLDTFLADQ
>Mature_221_residues
MEKSVTQENATPPVIGQRFRGFLPVVVDVETAGFNAQTDALLEIACIPIVYNEQGLFVPGESYHAHINPFEGANLDRRSL
DFIGIDPFNPMRIAMAEDERSALKRIFKSIHEVRKQQQCTHAVLVGHNAHFDLGFLQAAIARTSTKNQNPFHSFSVFDTV
TLSAVMFGQTVLAKACIQAGIDFDGKEAHSALYDTQKTAELFCYILNKLSPHLLDTFLADQ

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=203, Percent_Identity=53.2019704433498, Blast_Score=212, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.- [C]

Molecular weight: Translated: 24540; Mature: 24540

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSVTQENATPPVIGQRFRGFLPVVVDVETAGFNAQTDALLEIACIPIVYNEQGLFVPG
CCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHEEEEEEEEEEECCCCEEECC
ESYHAHINPFEGANLDRRSLDFIGIDPFNPMRIAMAEDERSALKRIFKSIHEVRKQQQCT
CCCEEECCCCCCCCCCCCCCCEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
HAVLVGHNAHFDLGFLQAAIARTSTKNQNPFHSFSVFDTVTLSAVMFGQTVLAKACIQAG
HEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IDFDGKEAHSALYDTQKTAELFCYILNKLSPHLLDTFLADQ
CCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MEKSVTQENATPPVIGQRFRGFLPVVVDVETAGFNAQTDALLEIACIPIVYNEQGLFVPG
CCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHEEEEEEEEEEECCCCEEECC
ESYHAHINPFEGANLDRRSLDFIGIDPFNPMRIAMAEDERSALKRIFKSIHEVRKQQQCT
CCCEEECCCCCCCCCCCCCCCEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
HAVLVGHNAHFDLGFLQAAIARTSTKNQNPFHSFSVFDTVTLSAVMFGQTVLAKACIQAG
HEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IDFDGKEAHSALYDTQKTAELFCYILNKLSPHLLDTFLADQ
CCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA