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The map label for this gene is cysD

Identifier: 50084271

GI number: 50084271

Start: 1068319

End: 1069233

Strand: Direct

Name: cysD

Synonym: ACIAD1072

Alternate gene names: 50084271

Gene position: 1068319-1069233 (Clockwise)

Preceding gene: 50084268

Following gene: 50084272

Centisome position: 29.69

GC content: 42.62

Gene sequence:

>915_bases
ATGCTCATGACTGAAGATAGATTAACCCATCTCAAGCAGCTTGAAGCTGAGAGTATTCATATTATTCGTGAAGTTGCTGC
CGAATTTGAAAACCCAGTGATGCTTTACTCGATTGGTAAAGACTCTGCGGTAATGCTGCATCTTGCACTCAAAGCTTTTT
ATCCTGCCAAACTTCCATTTCCACTGTTGCATGTTGATACAGGCTGGAAATTTAAAGACATGATTGCCTTTCGTGACAAC
ATGGCAAAAACCCATGGTTTTGACTTGATTGTGCATCAAAACAAAGAAGGCCGTGAAGCAGGCATTAACCCATTTGATCA
TGGTAGTTCAAAATATACCGACATCATGAAAACTCAAGGCTTAAAGCAAGCGTTGGATAAATATCAGTTTGATGCTGCGT
TTGGTGGGGCACGTCGTGATGAAGAAAAATCACGGGCCAAAGAGCGTGTTTACTCTTTCCGTGATACCAAACACCGCTGG
GATCCTAAAAATCAACGTCCAGAACTGTGGAGTCTTTATAACGGTAAAGTGAACAAAGGCGAAAGCATTCGTGTATTCCC
ATTGTCGAACTGGACTGAGCTGGATATCTGGCAATATATTTACCTTGAAAATATTCAAATCGTACCGTTGTATTTCTCTG
CGGTACGTCCTGTGGTTGAGCGTAGTGGTACGCTGATCATGGTCGATGATGAACGCATGCGCTTAAAAGAAGGCGAAGTT
CCGCAAATGAAATCGGTACGTTTCCGTACGCTTGGCTGTTATCCATTAACAGGTGCTGTGGAATCTGAGGCAGATACTTT
GCCTGAGATTATTCAGGAAATGCTTTTGGCCACAAGTTCAGAACGTCAAGGTCGTATGATTGACCATGATGAAGCGGGCT
CAATGGAAAAGAAAAAGCAGGAAGGTTATTTCTAA

Upstream 100 bases:

>100_bases
TAATTTGGAGTTGTTATGGCACTCGTTCCTCCATATGGTGGCGCAATTAGTGGTGAAAAACTACTAAGCGGCTTAATGAT
TAAAACGGACTGGAAAGGAG

Downstream 100 bases:

>100_bases
GCAGTTTTTATAAACGCTTGGGAAACGATTTCAGAATTTGTGGAGTTTGAGCATGTCTCACCAGTCAGATCTTATTAGCC
AAGACATCTTGGCATATTTA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MLMTEDRLTHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLALKAFYPAKLPFPLLHVDTGWKFKDMIAFRDN
MAKTHGFDLIVHQNKEGREAGINPFDHGSSKYTDIMKTQGLKQALDKYQFDAAFGGARRDEEKSRAKERVYSFRDTKHRW
DPKNQRPELWSLYNGKVNKGESIRVFPLSNWTELDIWQYIYLENIQIVPLYFSAVRPVVERSGTLIMVDDERMRLKEGEV
PQMKSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLATSSERQGRMIDHDEAGSMEKKKQEGYF

Sequences:

>Translated_304_residues
MLMTEDRLTHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLALKAFYPAKLPFPLLHVDTGWKFKDMIAFRDN
MAKTHGFDLIVHQNKEGREAGINPFDHGSSKYTDIMKTQGLKQALDKYQFDAAFGGARRDEEKSRAKERVYSFRDTKHRW
DPKNQRPELWSLYNGKVNKGESIRVFPLSNWTELDIWQYIYLENIQIVPLYFSAVRPVVERSGTLIMVDDERMRLKEGEV
PQMKSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLATSSERQGRMIDHDEAGSMEKKKQEGYF
>Mature_304_residues
MLMTEDRLTHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLALKAFYPAKLPFPLLHVDTGWKFKDMIAFRDN
MAKTHGFDLIVHQNKEGREAGINPFDHGSSKYTDIMKTQGLKQALDKYQFDAAFGGARRDEEKSRAKERVYSFRDTKHRW
DPKNQRPELWSLYNGKVNKGESIRVFPLSNWTELDIWQYIYLENIQIVPLYFSAVRPVVERSGTLIMVDDERMRLKEGEV
PQMKSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLATSSERQGRMIDHDEAGSMEKKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=76.158940397351, Blast_Score=489, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_ACIAD (Q6FDA0)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045781.1
- HSSP:   Q87WW0
- ProteinModelPortal:   Q6FDA0
- SMR:   Q6FDA0
- STRING:   Q6FDA0
- GeneID:   2878419
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1072
- NMPDR:   fig|62977.3.peg.1220
- HOGENOM:   HBG758022
- OMA:   NPQSTKP
- PhylomeDB:   Q6FDA0
- ProtClustDB:   PRK05253
- BioCyc:   ASP62977:ACIAD1072-MONOMER
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 35228; Mature: 35228

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMTEDRLTHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLALKAFYPAKLPF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCC
PLLHVDTGWKFKDMIAFRDNMAKTHGFDLIVHQNKEGREAGINPFDHGSSKYTDIMKTQG
CEEEECCCCCHHHHHHHHHHCHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
LKQALDKYQFDAAFGGARRDEEKSRAKERVYSFRDTKHRWDPKNQRPELWSLYNGKVNKG
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCC
ESIRVFPLSNWTELDIWQYIYLENIQIVPLYFSAVRPVVERSGTLIMVDDERMRLKEGEV
CEEEEEECCCCCCHHHHEEEECCCEEEEEHHHHHHHHHHHCCCCEEEECCCHHHHCCCCC
PQMKSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLATSSERQGRMIDHDEAGSMEKKKQ
CCHHCCEEEEECCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
EGYF
CCCC
>Mature Secondary Structure
MLMTEDRLTHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLALKAFYPAKLPF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCC
PLLHVDTGWKFKDMIAFRDNMAKTHGFDLIVHQNKEGREAGINPFDHGSSKYTDIMKTQG
CEEEECCCCCHHHHHHHHHHCHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
LKQALDKYQFDAAFGGARRDEEKSRAKERVYSFRDTKHRWDPKNQRPELWSLYNGKVNKG
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCC
ESIRVFPLSNWTELDIWQYIYLENIQIVPLYFSAVRPVVERSGTLIMVDDERMRLKEGEV
CEEEEEECCCCCCHHHHEEEECCCEEEEEHHHHHHHHHHHCCCCEEEECCCHHHHCCCCC
PQMKSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLATSSERQGRMIDHDEAGSMEKKKQ
CCHHCCEEEEECCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
EGYF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA