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The map label for this gene is cysN [H]

Identifier: 50084272

GI number: 50084272

Start: 1069286

End: 1070899

Strand: Direct

Name: cysN [H]

Synonym: ACIAD1073

Alternate gene names: 50084272

Gene position: 1069286-1070899 (Clockwise)

Preceding gene: 50084271

Following gene: 50084274

Centisome position: 29.71

GC content: 42.32

Gene sequence:

>1614_bases
ATGTCTCACCAGTCAGATCTTATTAGCCAAGACATCTTGGCATATTTAAAACAGCATGAAGAAAAAGACCTATTACGTTT
TTTGACTTGCGGTAACGTTGATGATGGTAAAAGTACGCTGATTGGACGTTTGTTATATGATTCAAAATTGATCTATGAAG
ATCAATTACAAGCCGTAACGCGTGATAGTAAAAAAGTTGGGACGACAGGTGATGCACCTGATTTGGCATTGTTGGTCGAT
GGCTTGCAAGCTGAACGTGAACAGGGTATTACCATTGATGTGGCGTATCGTTACTTTTCTACCGAAAAGCGTAAATTCAT
CATTGCTGATACACCAGGGCATGAGCAGTATACCCGCAATATGGCAACAGGTGCATCAACGGCCGATCTTGCGATTATCC
TGATTGATGCACGTTATGGCGTACAAACCCAAACGCGTCGTCATACCTTTATTGCGAGCTTATTGGGTATTCGTAATATC
GTGGTCGCGATTAACAAAATGGACTTGATCGAGTTTTCAGAACCACGTTTCAATGAAATTCAGGTTGAATATGATGCGTT
TGTCAACCAGTTAGGTGATCGTCGTCCAGAGAATATCTTATTCGTTCCAATTTCGGCATTGAATGGTGACAATGTCGTTA
ATCCATCGGAAAGTACGCCGTGGTACAAGGGTCAAACCTTGATGAGCATTTTGGAATCTGTGGAAATTAAACGTGAATCG
AATAAACACGAGTTCCGTTTCCCTGTTCAGTATGTAAACCGTCCAAATCTGGATTTCCGTGGTTTTGCTGGAACAATTGC
GCTTGGTGAGATTCATGTTGGTGATGAGATTATTGCATTACCATCAGGTAAAAAATCCAGTGTTAAAGAGATTGTGACTT
TTGATGGCAATCTTGAGCAAGCATTTGCGGGGCAAGCTGTCACATTAACACTCAATGATGAGATTGATGTTTCGCGTGGT
AATGTTTTAGTTCGTGCAGGTGAGCAGCCACTCATTTCACGAAGTGTTCGTGCTTCTGTGGTATGGATGAACGAACATCC
TTTAGTAAAAGGCAAGCTATATAACGTCAAAATCGGCACACAAACAGTTCCTGCTAAAGTGACTGAGATTAACTATCGTG
TCAATGTCAACACGCTTGAGCATACACAAGTTGAAGAGCTTGAACTGAATGCCATTGCAGATGTAACAGTTGAGTTTGAT
GCGCCAGTGGTGTTTGATCGTTATCAGGACTCACGTTATACAGGTTCATTTATCTTTATTGACCGCTTAAGCAACGTCAC
CGTGGGTGCTGGTATGGTTGAGGCGGCAGTTGAATGGACTGCTCACAGCAATCCTGTAACCGCCGAAGATCGTGCTGCGC
GTTTAGGTCAAAAACCTGCCGTGATTGGTGTGTCTGCAAATATTCTTGAGCAGTCACAAGTGCTTGAGAGCTTGTTGATT
CAACAAGGTGTTGTAGCAATTGCCAAAGCTGGTTTGACACTTGAGCAGATTGGTTTATTACGTGAAACGGGTGTTGTTGT
GATAACGACACAAGTAGAAGCTACAGATACCAATGTAAATGCAACAACTGTTGAAGATGTGGTTGATCAAGTGGTTGAAT
TGGTTCGCCTATAA

Upstream 100 bases:

>100_bases
GATGAAGCGGGCTCAATGGAAAAGAAAAAGCAGGAAGGTTATTTCTAAGCAGTTTTTATAAACGCTTGGGAAACGATTTC
AGAATTTGTGGAGTTTGAGC

Downstream 100 bases:

>100_bases
TTATCTTTATCATAAAAAACCTCCTAAATTTAGGAGGTTTTTTTGTAGCTTCAAGCTTAGTTTAAATCATGCTTACCAAT
GGGAATACACCAAATACCAA

Product: ATP-sulfurylase, subunit 1

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSHQSDLISQDILAYLKQHEEKDLLRFLTCGNVDDGKSTLIGRLLYDSKLIYEDQLQAVTRDSKKVGTTGDAPDLALLVD
GLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTADLAIILIDARYGVQTQTRRHTFIASLLGIRNI
VVAINKMDLIEFSEPRFNEIQVEYDAFVNQLGDRRPENILFVPISALNGDNVVNPSESTPWYKGQTLMSILESVEIKRES
NKHEFRFPVQYVNRPNLDFRGFAGTIALGEIHVGDEIIALPSGKKSSVKEIVTFDGNLEQAFAGQAVTLTLNDEIDVSRG
NVLVRAGEQPLISRSVRASVVWMNEHPLVKGKLYNVKIGTQTVPAKVTEINYRVNVNTLEHTQVEELELNAIADVTVEFD
APVVFDRYQDSRYTGSFIFIDRLSNVTVGAGMVEAAVEWTAHSNPVTAEDRAARLGQKPAVIGVSANILEQSQVLESLLI
QQGVVAIAKAGLTLEQIGLLRETGVVVITTQVEATDTNVNATTVEDVVDQVVELVRL

Sequences:

>Translated_537_residues
MSHQSDLISQDILAYLKQHEEKDLLRFLTCGNVDDGKSTLIGRLLYDSKLIYEDQLQAVTRDSKKVGTTGDAPDLALLVD
GLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTADLAIILIDARYGVQTQTRRHTFIASLLGIRNI
VVAINKMDLIEFSEPRFNEIQVEYDAFVNQLGDRRPENILFVPISALNGDNVVNPSESTPWYKGQTLMSILESVEIKRES
NKHEFRFPVQYVNRPNLDFRGFAGTIALGEIHVGDEIIALPSGKKSSVKEIVTFDGNLEQAFAGQAVTLTLNDEIDVSRG
NVLVRAGEQPLISRSVRASVVWMNEHPLVKGKLYNVKIGTQTVPAKVTEINYRVNVNTLEHTQVEELELNAIADVTVEFD
APVVFDRYQDSRYTGSFIFIDRLSNVTVGAGMVEAAVEWTAHSNPVTAEDRAARLGQKPAVIGVSANILEQSQVLESLLI
QQGVVAIAKAGLTLEQIGLLRETGVVVITTQVEATDTNVNATTVEDVVDQVVELVRL
>Mature_536_residues
SHQSDLISQDILAYLKQHEEKDLLRFLTCGNVDDGKSTLIGRLLYDSKLIYEDQLQAVTRDSKKVGTTGDAPDLALLVDG
LQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTADLAIILIDARYGVQTQTRRHTFIASLLGIRNIV
VAINKMDLIEFSEPRFNEIQVEYDAFVNQLGDRRPENILFVPISALNGDNVVNPSESTPWYKGQTLMSILESVEIKRESN
KHEFRFPVQYVNRPNLDFRGFAGTIALGEIHVGDEIIALPSGKKSSVKEIVTFDGNLEQAFAGQAVTLTLNDEIDVSRGN
VLVRAGEQPLISRSVRASVVWMNEHPLVKGKLYNVKIGTQTVPAKVTEINYRVNVNTLEHTQVEELELNAIADVTVEFDA
PVVFDRYQDSRYTGSFIFIDRLSNVTVGAGMVEAAVEWTAHSNPVTAEDRAARLGQKPAVIGVSANILEQSQVLESLLIQ
QGVVAIAKAGLTLEQIGLLRETGVVVITTQVEATDTNVNATTVEDVVDQVVELVRL

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=31.9727891156463, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=31.9727891156463, Blast_Score=192, Evalue=8e-49,
Organism=Homo sapiens, GI4503471, Length=475, Percent_Identity=28.2105263157895, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=28.4424379232506, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI194018522, Length=419, Percent_Identity=26.491646778043, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI194018520, Length=419, Percent_Identity=26.491646778043, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI194097354, Length=419, Percent_Identity=26.491646778043, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI46094014, Length=419, Percent_Identity=25.7756563245823, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI34147630, Length=314, Percent_Identity=28.9808917197452, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI157426893, Length=346, Percent_Identity=26.0115606936416, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789108, Length=426, Percent_Identity=61.7370892018779, Blast_Score=530, Evalue=1e-151,
Organism=Escherichia coli, GI1790412, Length=314, Percent_Identity=28.9808917197452, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789737, Length=314, Percent_Identity=28.9808917197452, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367247, Length=304, Percent_Identity=25.6578947368421, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=29.1666666666667, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=28.8018433179723, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=30.1339285714286, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=30.1339285714286, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI32566629, Length=432, Percent_Identity=27.0833333333333, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=28.9954337899543, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=34.8993288590604, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI25141371, Length=282, Percent_Identity=26.9503546099291, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=348, Percent_Identity=27.8735632183908, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=355, Percent_Identity=32.3943661971831, Blast_Score=180, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6319594, Length=355, Percent_Identity=32.3943661971831, Blast_Score=180, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6322937, Length=423, Percent_Identity=30.2600472813239, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320377, Length=432, Percent_Identity=28.2407407407407, Blast_Score=133, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6324761, Length=316, Percent_Identity=29.746835443038, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45553807, Length=446, Percent_Identity=28.47533632287, Blast_Score=168, Evalue=8e-42,
Organism=Drosophila melanogaster, GI45553816, Length=446, Percent_Identity=28.47533632287, Blast_Score=168, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24651721, Length=446, Percent_Identity=28.47533632287, Blast_Score=168, Evalue=8e-42,
Organism=Drosophila melanogaster, GI17864154, Length=446, Percent_Identity=28.47533632287, Blast_Score=168, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24652838, Length=475, Percent_Identity=28.2105263157895, Blast_Score=166, Evalue=5e-41,
Organism=Drosophila melanogaster, GI17137572, Length=475, Percent_Identity=28.2105263157895, Blast_Score=166, Evalue=5e-41,
Organism=Drosophila melanogaster, GI45550900, Length=431, Percent_Identity=27.3781902552204, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17137380, Length=334, Percent_Identity=27.2455089820359, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI281363316, Length=317, Percent_Identity=29.6529968454259, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17864358, Length=317, Percent_Identity=29.6529968454259, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19921738, Length=396, Percent_Identity=26.010101010101, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 59398; Mature: 59267

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQSDLISQDILAYLKQHEEKDLLRFLTCGNVDDGKSTLIGRLLYDSKLIYEDQLQAVT
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHH
RDSKKVGTTGDAPDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRN
CCCCCCCCCCCCCCCEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHH
MATGASTADLAIILIDARYGVQTQTRRHTFIASLLGIRNIVVAINKMDLIEFSEPRFNEI
HHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEEEEECEEEEECCCCCCCEE
QVEYDAFVNQLGDRRPENILFVPISALNGDNVVNPSESTPWYKGQTLMSILESVEIKRES
EEHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
NKHEFRFPVQYVNRPNLDFRGFAGTIALGEIHVGDEIIALPSGKKSSVKEIVTFDGNLEQ
CCCEEECCHHHCCCCCCCCCCCCCEEEEEEEEECCEEEEECCCCCCCCCEEEEECCCHHH
AFAGQAVTLTLNDEIDVSRGNVLVRAGEQPLISRSVRASVVWMNEHPLVKGKLYNVKIGT
HHCCCEEEEEECCCEEECCCCEEEEECCCCHHHHCCEEEEEEECCCCEEEEEEEEEEECC
QTVPAKVTEINYRVNVNTLEHTQVEELELNAIADVTVEFDAPVVFDRYQDSRYTGSFIFI
CCCCCEEEEEEEEEEECCCCCCCHHHEEEEEEEEEEEEECCCEEEECCCCCCCCCCEEEE
DRLSNVTVGAGMVEAAVEWTAHSNPVTAEDRAARLGQKPAVIGVSANILEQSQVLESLLI
EECCCCEECCCHHHHHHEECCCCCCCCCCHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
QQGVVAIAKAGLTLEQIGLLRETGVVVITTQVEATDTNVNATTVEDVVDQVVELVRL
HHCHHHHHHCCCCHHHHHHHHHCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHQSDLISQDILAYLKQHEEKDLLRFLTCGNVDDGKSTLIGRLLYDSKLIYEDQLQAVT
CCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHH
RDSKKVGTTGDAPDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRN
CCCCCCCCCCCCCCCEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHH
MATGASTADLAIILIDARYGVQTQTRRHTFIASLLGIRNIVVAINKMDLIEFSEPRFNEI
HHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEEEEECEEEEECCCCCCCEE
QVEYDAFVNQLGDRRPENILFVPISALNGDNVVNPSESTPWYKGQTLMSILESVEIKRES
EEHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
NKHEFRFPVQYVNRPNLDFRGFAGTIALGEIHVGDEIIALPSGKKSSVKEIVTFDGNLEQ
CCCEEECCHHHCCCCCCCCCCCCCEEEEEEEEECCEEEEECCCCCCCCCEEEEECCCHHH
AFAGQAVTLTLNDEIDVSRGNVLVRAGEQPLISRSVRASVVWMNEHPLVKGKLYNVKIGT
HHCCCEEEEEECCCEEECCCCEEEEECCCCHHHHCCEEEEEEECCCCEEEEEEEEEEECC
QTVPAKVTEINYRVNVNTLEHTQVEELELNAIADVTVEFDAPVVFDRYQDSRYTGSFIFI
CCCCCEEEEEEEEEEECCCCCCCHHHEEEEEEEEEEEEECCCEEEECCCCCCCCCCEEEE
DRLSNVTVGAGMVEAAVEWTAHSNPVTAEDRAARLGQKPAVIGVSANILEQSQVLESLLI
EECCCCEECCCHHHHHHEECCCCCCCCCCHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
QQGVVAIAKAGLTLEQIGLLRETGVVVITTQVEATDTNVNATTVEDVVDQVVELVRL
HHCHHHHHHCCCCHHHHHHHHHCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9611812; 10984043 [H]