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The map label for this gene is lysS

Identifier: 50084268

GI number: 50084268

Start: 1064137

End: 1065666

Strand: Direct

Name: lysS

Synonym: ACIAD1069

Alternate gene names: 50084268

Gene position: 1064137-1065666 (Clockwise)

Preceding gene: 50084262

Following gene: 50084271

Centisome position: 29.57

GC content: 40.72

Gene sequence:

>1530_bases
ATGGCGCAACCAAACGTTCAATCGACTTCCGAACCTATTCTTTCCGAAAACGATTTAATTGCACAGCGTCATGCCAAATT
AAAACAAATTCAAGACAAAGCCAAAGAAACTGGCAAAAGCGTTTGGCCGAATACCTTTAAACGTGAGCATTATGCTGCTG
ACCTGCAAGAGCAGTTTAAAGATATTGATAAGGCACAGATTGAAGGTTCAGACAAAACCTATGTAAAAGTTGCTGGTCGT
GTCATGCTCAATCGTGGATCATTTATGGTGATCCAGGATATGACGGGTCGTATCCAGCTCTACGTTGACCGTAAAGGCCT
ACCAGCAGATACTTTAGAAACCATTAAGAGTCTTGACCTTGGCGATATTATTGCTGCGGAAGGTTATATTGGGCGTTCGG
GTAAAGGTGATCTATATGTTCACCTTGAAGGTTTTGAGTTATTAACCAAGTCTTTGCGTCCACTACCAGATAAGTTTCAT
GGTCTGACTGATACAGAGGCGAAATATCGCAAGCGTTATCTTGATTTGATCGTGAACGAAGAAACACGTAAAACGTTTGA
GATTCGTGCTCAAGTTGTTGCTGGTATTCGTGCATTTTTGACCAATCAACGTTTTATGGAAGTTGAAACGCCAATGATGC
ATGTGATTCCAGGTGGTGCATCTGCACAACCATTTGTGACACATCATAATGCTTTGGATATGGAGCTGTATTTACGTATT
GCACCAGAACTTTATCTTAAGCGTTTGGTTGTGGGTGGTTTTGAGCGTGTCTTTGAAATCAACCGTAACTTCCGTAATGA
AGGGGTTTCAACACGTCATAATCCTGAATTTACCATGATTGAGTTTTATCAGGCATATGCAGATTATAAAGATTTAATGC
AACTGACTGAAAATATGCTTGAAAAACTTGCATTAGATATTTTGGGAACAACCGATGTTCCTTATGGCGATGAAGTGTAT
AGCTTTAAAGGGCCATTCAAAAAAATCTCGATGTTTGATGCGATTTTGGAACATAACCCAGATTTCACCCCTGAAAATGT
CAATGATCGTGAGTTTTTAGCCAAATTCACTCAAGACGTGCTTAAAGAGCAAGTTAAACCTGGTTTTGGTTTAGGCAAGT
TACAAACGATTGTGTTTGAAGAAACCGTTGAAACTAAATTGCGTCAGCCTACATTTATTACCGAATATCCAGCAGAAACC
TCACCGCTGGCACGTCGTAATGATGATAATCCTCACATCACAGACCGTTTCGAATTCTTTATTGGTGGTCGTGAACTTGC
AAATGGTTTCTCGGAGCTGAATGATCCGATTGATCAGGCAGAACGTTTTCAGGCTCAAGTTGCCGAAAAAGATGCAGGTG
ATGATGAAGCCATGCATTATGATGCAGACTTTATTGAAGCACTTGAATACGGTTTACCGCCAACAGCGGGTCAGGGTATC
GGGATTGATCGTCTGGTTATGATTTTTGCCAATGCACCAAGTATTCGTGATGTACTGTTATTTCCGCATATGCGTCGTAA
AGATGTATAA

Upstream 100 bases:

>100_bases
AAAATAACTTTTTTAATGATGAACTGGCAAGAAGACATACGCAATTGTAGTGAGTTCTGTTAAGCTATACGACTTCATTT
TTTAATTGACCGAGGCAGAG

Downstream 100 bases:

>100_bases
ATAAAAAAGATATAAAAAGAGCTTCTAATTCAGAAGCTCTTTTTTTTGCTGAAAATTATTTTTTAATGGCTTCGTTGTAA
CGCTCATTAACTTTATTCCA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MAQPNVQSTSEPILSENDLIAQRHAKLKQIQDKAKETGKSVWPNTFKREHYAADLQEQFKDIDKAQIEGSDKTYVKVAGR
VMLNRGSFMVIQDMTGRIQLYVDRKGLPADTLETIKSLDLGDIIAAEGYIGRSGKGDLYVHLEGFELLTKSLRPLPDKFH
GLTDTEAKYRKRYLDLIVNEETRKTFEIRAQVVAGIRAFLTNQRFMEVETPMMHVIPGGASAQPFVTHHNALDMELYLRI
APELYLKRLVVGGFERVFEINRNFRNEGVSTRHNPEFTMIEFYQAYADYKDLMQLTENMLEKLALDILGTTDVPYGDEVY
SFKGPFKKISMFDAILEHNPDFTPENVNDREFLAKFTQDVLKEQVKPGFGLGKLQTIVFEETVETKLRQPTFITEYPAET
SPLARRNDDNPHITDRFEFFIGGRELANGFSELNDPIDQAERFQAQVAEKDAGDDEAMHYDADFIEALEYGLPPTAGQGI
GIDRLVMIFANAPSIRDVLLFPHMRRKDV

Sequences:

>Translated_509_residues
MAQPNVQSTSEPILSENDLIAQRHAKLKQIQDKAKETGKSVWPNTFKREHYAADLQEQFKDIDKAQIEGSDKTYVKVAGR
VMLNRGSFMVIQDMTGRIQLYVDRKGLPADTLETIKSLDLGDIIAAEGYIGRSGKGDLYVHLEGFELLTKSLRPLPDKFH
GLTDTEAKYRKRYLDLIVNEETRKTFEIRAQVVAGIRAFLTNQRFMEVETPMMHVIPGGASAQPFVTHHNALDMELYLRI
APELYLKRLVVGGFERVFEINRNFRNEGVSTRHNPEFTMIEFYQAYADYKDLMQLTENMLEKLALDILGTTDVPYGDEVY
SFKGPFKKISMFDAILEHNPDFTPENVNDREFLAKFTQDVLKEQVKPGFGLGKLQTIVFEETVETKLRQPTFITEYPAET
SPLARRNDDNPHITDRFEFFIGGRELANGFSELNDPIDQAERFQAQVAEKDAGDDEAMHYDADFIEALEYGLPPTAGQGI
GIDRLVMIFANAPSIRDVLLFPHMRRKDV
>Mature_508_residues
AQPNVQSTSEPILSENDLIAQRHAKLKQIQDKAKETGKSVWPNTFKREHYAADLQEQFKDIDKAQIEGSDKTYVKVAGRV
MLNRGSFMVIQDMTGRIQLYVDRKGLPADTLETIKSLDLGDIIAAEGYIGRSGKGDLYVHLEGFELLTKSLRPLPDKFHG
LTDTEAKYRKRYLDLIVNEETRKTFEIRAQVVAGIRAFLTNQRFMEVETPMMHVIPGGASAQPFVTHHNALDMELYLRIA
PELYLKRLVVGGFERVFEINRNFRNEGVSTRHNPEFTMIEFYQAYADYKDLMQLTENMLEKLALDILGTTDVPYGDEVYS
FKGPFKKISMFDAILEHNPDFTPENVNDREFLAKFTQDVLKEQVKPGFGLGKLQTIVFEETVETKLRQPTFITEYPAETS
PLARRNDDNPHITDRFEFFIGGRELANGFSELNDPIDQAERFQAQVAEKDAGDDEAMHYDADFIEALEYGLPPTAGQGIG
IDRLVMIFANAPSIRDVLLFPHMRRKDV

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=492, Percent_Identity=40.4471544715447, Blast_Score=353, Evalue=3e-97,
Organism=Homo sapiens, GI194272210, Length=492, Percent_Identity=40.4471544715447, Blast_Score=353, Evalue=3e-97,
Organism=Homo sapiens, GI40789249, Length=241, Percent_Identity=25.7261410788382, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789256, Length=510, Percent_Identity=55.8823529411765, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1790571, Length=510, Percent_Identity=55.6862745098039, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI87082379, Length=323, Percent_Identity=32.1981424148607, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788173, Length=158, Percent_Identity=27.2151898734177, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=494, Percent_Identity=41.497975708502, Blast_Score=354, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI17535927, Length=494, Percent_Identity=41.497975708502, Blast_Score=354, Evalue=8e-98,
Organism=Caenorhabditis elegans, GI71994340, Length=494, Percent_Identity=41.497975708502, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32566633, Length=305, Percent_Identity=25.9016393442623, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=507, Percent_Identity=40.6311637080868, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324256, Length=425, Percent_Identity=31.7647058823529, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321807, Length=453, Percent_Identity=23.6203090507726, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24640851, Length=517, Percent_Identity=40.2321083172147, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640849, Length=517, Percent_Identity=40.2321083172147, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24584738, Length=284, Percent_Identity=23.943661971831, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_ACIAD (Q43990)

Other databases:

- EMBL:   Z46863
- EMBL:   CR543861
- RefSeq:   YP_045778.1
- ProteinModelPortal:   Q43990
- SMR:   Q43990
- STRING:   Q43990
- GeneID:   2878610
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1069
- NMPDR:   fig|62977.3.peg.1217
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- PhylomeDB:   Q43990
- ProtClustDB:   PRK00484
- BioCyc:   ASP62977:ACIAD1069-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 58080; Mature: 57949

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPNVQSTSEPILSENDLIAQRHAKLKQIQDKAKETGKSVWPNTFKREHYAADLQEQFK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DIDKAQIEGSDKTYVKVAGRVMLNRGSFMVIQDMTGRIQLYVDRKGLPADTLETIKSLDL
HHHHHHCCCCCCEEEEEECCCEECCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHCCCC
GDIIAAEGYIGRSGKGDLYVHLEGFELLTKSLRPLPDKFHGLTDTEAKYRKRYLDLIVNE
CCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCC
ETRKTFEIRAQVVAGIRAFLTNQRFMEVETPMMHVIPGGASAQPFVTHHNALDMELYLRI
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCEEECCCCCCHHHHHHH
APELYLKRLVVGGFERVFEINRNFRNEGVSTRHNPEFTMIEFYQAYADYKDLMQLTENML
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EKLALDILGTTDVPYGDEVYSFKGPFKKISMFDAILEHNPDFTPENVNDREFLAKFTQDV
HHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LKEQVKPGFGLGKLQTIVFEETVETKLRQPTFITEYPAETSPLARRNDDNPHITDRFEFF
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHEE
IGGRELANGFSELNDPIDQAERFQAQVAEKDAGDDEAMHYDADFIEALEYGLPPTAGQGI
ECCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHCCCCCCCCCC
GIDRLVMIFANAPSIRDVLLFPHMRRKDV
CHHHHEEEEECCCCHHHHHHCCCHHHCCC
>Mature Secondary Structure 
AQPNVQSTSEPILSENDLIAQRHAKLKQIQDKAKETGKSVWPNTFKREHYAADLQEQFK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DIDKAQIEGSDKTYVKVAGRVMLNRGSFMVIQDMTGRIQLYVDRKGLPADTLETIKSLDL
HHHHHHCCCCCCEEEEEECCCEECCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHCCCC
GDIIAAEGYIGRSGKGDLYVHLEGFELLTKSLRPLPDKFHGLTDTEAKYRKRYLDLIVNE
CCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCC
ETRKTFEIRAQVVAGIRAFLTNQRFMEVETPMMHVIPGGASAQPFVTHHNALDMELYLRI
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCEEECCCCCCHHHHHHH
APELYLKRLVVGGFERVFEINRNFRNEGVSTRHNPEFTMIEFYQAYADYKDLMQLTENML
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EKLALDILGTTDVPYGDEVYSFKGPFKKISMFDAILEHNPDFTPENVNDREFLAKFTQDV
HHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LKEQVKPGFGLGKLQTIVFEETVETKLRQPTFITEYPAETSPLARRNDDNPHITDRFEFF
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHEE
IGGRELANGFSELNDPIDQAERFQAQVAEKDAGDDEAMHYDADFIEALEYGLPPTAGQGI
ECCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHCCCCCCCCCC
GIDRLVMIFANAPSIRDVLLFPHMRRKDV
CHHHHEEEEECCCCHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9074511