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The map label for this gene is def2 [H]

Identifier: 50084241

GI number: 50084241

Start: 1029904

End: 1030386

Strand: Direct

Name: def2 [H]

Synonym: ACIAD1039

Alternate gene names: 50084241

Gene position: 1029904-1030386 (Clockwise)

Preceding gene: 50084240

Following gene: 50084242

Centisome position: 28.62

GC content: 44.72

Gene sequence:

>483_bases
ATGAGCACTGTATTAACGGTTGCAAAGCGCGGCGAAGAGATTTTAAAGCTTAACGCTGCGCCTGTGTCTGAGCAGGAATT
TGACAGTGAGTGGTTGCAACAACTGGTAAAGGCAATGCAAGCCACCATGCTTGAGCGAAATGGAGTAGGGATTGCTGCGC
CACAAATATATGTTTCCAAAAGAATCATGATTGTGGCATCGCGTCCCAATCCACGTTATCCCGATGCACCTGAAATGCAA
CCTGTGGTGATGATTAATCCCGAGATTACGCATTTTTCTTTTGAAAAAGAGCTTGGAGAGGAGGGCTGTTTGAGCGTACC
AGATCAGCGTGGTCAGGTTGAGCGTGCACAAAGTATAGATGTTCGTTATTTTAGCTTACAAGGCCAGTTGATTGAACAGC
GCTTTCATGGCTTTCCTGCACGTATTGTGCAACATGAAATTGATCATCTCAATGGCGTACTATTTGTCGACAGATTGATT
TAA

Upstream 100 bases:

>100_bases
AAAGCAATGAGCAAGAAGGTGTTCTGGATATTATTGATCTGGTTTTACAATCGAAAGGCTTTTCACACAGTTTAGTGTCT
GAACCTGAGGTGATGTCTTA

Downstream 100 bases:

>100_bases
TCTGTCTGGTTTTTTTGCTTAGAGAGTTTGCCCAATGTGTTGTTTAGAGAACTAAAGTAGTAAAACAAATCGCTATATTA
CAAAAGACCTGATTTTAGTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MSTVLTVAKRGEEILKLNAAPVSEQEFDSEWLQQLVKAMQATMLERNGVGIAAPQIYVSKRIMIVASRPNPRYPDAPEMQ
PVVMINPEITHFSFEKELGEEGCLSVPDQRGQVERAQSIDVRYFSLQGQLIEQRFHGFPARIVQHEIDHLNGVLFVDRLI

Sequences:

>Translated_160_residues
MSTVLTVAKRGEEILKLNAAPVSEQEFDSEWLQQLVKAMQATMLERNGVGIAAPQIYVSKRIMIVASRPNPRYPDAPEMQ
PVVMINPEITHFSFEKELGEEGCLSVPDQRGQVERAQSIDVRYFSLQGQLIEQRFHGFPARIVQHEIDHLNGVLFVDRLI
>Mature_159_residues
STVLTVAKRGEEILKLNAAPVSEQEFDSEWLQQLVKAMQATMLERNGVGIAAPQIYVSKRIMIVASRPNPRYPDAPEMQP
VVMINPEITHFSFEKELGEEGCLSVPDQRGQVERAQSIDVRYFSLQGQLIEQRFHGFPARIVQHEIDHLNGVLFVDRLI

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=29.6296296296296, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1789682, Length=157, Percent_Identity=36.9426751592357, Blast_Score=89, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=31.6455696202532, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24645726, Length=158, Percent_Identity=33.5443037974684, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18156; Mature: 18025

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVLTVAKRGEEILKLNAAPVSEQEFDSEWLQQLVKAMQATMLERNGVGIAAPQIYVSK
CCHHHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEE
RIMIVASRPNPRYPDAPEMQPVVMINPEITHFSFEKELGEEGCLSVPDQRGQVERAQSID
EEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCCCCCCCCCCCCCHHHHHCCC
VRYFSLQGQLIEQRFHGFPARIVQHEIDHLNGVLFVDRLI
EEEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
STVLTVAKRGEEILKLNAAPVSEQEFDSEWLQQLVKAMQATMLERNGVGIAAPQIYVSK
CHHHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEE
RIMIVASRPNPRYPDAPEMQPVVMINPEITHFSFEKELGEEGCLSVPDQRGQVERAQSID
EEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCCCCCCCCCCCCCHHHHHCCC
VRYFSLQGQLIEQRFHGFPARIVQHEIDHLNGVLFVDRLI
EEEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]