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The map label for this gene is ybjI [H]

Identifier: 50084240

GI number: 50084240

Start: 1029053

End: 1029904

Strand: Direct

Name: ybjI [H]

Synonym: ACIAD1038

Alternate gene names: 50084240

Gene position: 1029053-1029904 (Clockwise)

Preceding gene: 50084237

Following gene: 50084241

Centisome position: 28.6

GC content: 39.67

Gene sequence:

>852_bases
ATGCCAGTAAAACTTATCGCAGTCGATATGGATGGAACGTTTTTAAATTCAGAGAAGAAATACAATAAAGCGCGATTTTT
AAAACAATATGCGCAGCTTAAACAACGCGGAATAAAATTCGTTGCAGCAAGTGGCAATCCGCTTTATACCCTTCAAGCTT
ATTTTCCCGAAATTACCGATGAAATTGCATTCGTCGCGGAAAATGGTGCTTATGTCACAGCTGAGAATGAGGAAATTCAT
TACAGCGCCTATGCGCCAGAAGTATTAAAGCATATGGTGTCTGATCTCGAAGCTAAGTATGGAAGTAGTGTGATTTTATG
TGCTAAATCCTGCGCGTATGTCCGATCAGATGTGACAGCAAAAACACTTGCTAAACTGAATATCTATTTTAAAAGCATCA
AACAGGTCGATTCGCTACTTGATGTCAAAGAGCCAATTTGTAAAGTCACCCTTACCACTGTTGAACATGACTTTGAAGCA
ATGGAGCAGTATTTAAAACAGCAAGATTATGTCAAAAACCGTCTCACCAAAGCGGTATCAAGTGGTTTTGGTTTTATTGA
CTTGATTCAACCGAACAAGCACAAAGCCTATGGACTTGCATTCCTACAAGAAAGGTGGGGAATTGCGGATGAAGATATGC
TGACCATTGGTGATAATAATAATGATATCGAAATGATTCAGAAAGCGGGTTATGGGTTTGCCATGAGCAATGCAGTGCCA
GCACTAAAGCAAGTTGCAAAGTACCACGCCAAAAGCAATGAGCAAGAAGGTGTTCTGGATATTATTGATCTGGTTTTACA
ATCGAAAGGCTTTTCACACAGTTTAGTGTCTGAACCTGAGGTGATGTCTTAA

Upstream 100 bases:

>100_bases
AATAAGTAAAAAATAATGTGATCGCTTTCATAAAACTGTGATGTACTGATAGGATAATGAAGTAAAAGTTAATTTTTCTA
CAACTTACTATGAGGTATAA

Downstream 100 bases:

>100_bases
TGAGCACTGTATTAACGGTTGCAAAGCGCGGCGAAGAGATTTTAAAGCTTAACGCTGCGCCTGTGTCTGAGCAGGAATTT
GACAGTGAGTGGTTGCAACA

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MPVKLIAVDMDGTFLNSEKKYNKARFLKQYAQLKQRGIKFVAASGNPLYTLQAYFPEITDEIAFVAENGAYVTAENEEIH
YSAYAPEVLKHMVSDLEAKYGSSVILCAKSCAYVRSDVTAKTLAKLNIYFKSIKQVDSLLDVKEPICKVTLTTVEHDFEA
MEQYLKQQDYVKNRLTKAVSSGFGFIDLIQPNKHKAYGLAFLQERWGIADEDMLTIGDNNNDIEMIQKAGYGFAMSNAVP
ALKQVAKYHAKSNEQEGVLDIIDLVLQSKGFSHSLVSEPEVMS

Sequences:

>Translated_283_residues
MPVKLIAVDMDGTFLNSEKKYNKARFLKQYAQLKQRGIKFVAASGNPLYTLQAYFPEITDEIAFVAENGAYVTAENEEIH
YSAYAPEVLKHMVSDLEAKYGSSVILCAKSCAYVRSDVTAKTLAKLNIYFKSIKQVDSLLDVKEPICKVTLTTVEHDFEA
MEQYLKQQDYVKNRLTKAVSSGFGFIDLIQPNKHKAYGLAFLQERWGIADEDMLTIGDNNNDIEMIQKAGYGFAMSNAVP
ALKQVAKYHAKSNEQEGVLDIIDLVLQSKGFSHSLVSEPEVMS
>Mature_282_residues
PVKLIAVDMDGTFLNSEKKYNKARFLKQYAQLKQRGIKFVAASGNPLYTLQAYFPEITDEIAFVAENGAYVTAENEEIHY
SAYAPEVLKHMVSDLEAKYGSSVILCAKSCAYVRSDVTAKTLAKLNIYFKSIKQVDSLLDVKEPICKVTLTTVEHDFEAM
EQYLKQQDYVKNRLTKAVSSGFGFIDLIQPNKHKAYGLAFLQERWGIADEDMLTIGDNNNDIEMIQKAGYGFAMSNAVPA
LKQVAKYHAKSNEQEGVLDIIDLVLQSKGFSHSLVSEPEVMS

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=269, Percent_Identity=42.7509293680297, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1787043, Length=268, Percent_Identity=38.0597014925373, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI2367265, Length=269, Percent_Identity=27.5092936802974, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 31681; Mature: 31549

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKLIAVDMDGTFLNSEKKYNKARFLKQYAQLKQRGIKFVAASGNPLYTLQAYFPEITD
CCEEEEEEECCCCEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHHHHH
EIAFVAENGAYVTAENEEIHYSAYAPEVLKHMVSDLEAKYGSSVILCAKSCAYVRSDVTA
HHEEEECCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCHH
KTLAKLNIYFKSIKQVDSLLDVKEPICKVTLTTVEHDFEAMEQYLKQQDYVKNRLTKAVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGFGFIDLIQPNKHKAYGLAFLQERWGIADEDMLTIGDNNNDIEMIQKAGYGFAMSNAVP
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCEEHHHHHH
ALKQVAKYHAKSNEQEGVLDIIDLVLQSKGFSHSLVSEPEVMS
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCC
>Mature Secondary Structure 
PVKLIAVDMDGTFLNSEKKYNKARFLKQYAQLKQRGIKFVAASGNPLYTLQAYFPEITD
CEEEEEEECCCCEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHHHHH
EIAFVAENGAYVTAENEEIHYSAYAPEVLKHMVSDLEAKYGSSVILCAKSCAYVRSDVTA
HHEEEECCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCHH
KTLAKLNIYFKSIKQVDSLLDVKEPICKVTLTTVEHDFEAMEQYLKQQDYVKNRLTKAVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGFGFIDLIQPNKHKAYGLAFLQERWGIADEDMLTIGDNNNDIEMIQKAGYGFAMSNAVP
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCEEHHHHHH
ALKQVAKYHAKSNEQEGVLDIIDLVLQSKGFSHSLVSEPEVMS
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]