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The map label for this gene is ada [H]

Identifier: 50083608

GI number: 50083608

Start: 339360

End: 340220

Strand: Direct

Name: ada [H]

Synonym: ACIAD0344

Alternate gene names: 50083608

Gene position: 339360-340220 (Clockwise)

Preceding gene: 50083607

Following gene: 50083609

Centisome position: 9.43

GC content: 45.06

Gene sequence:

>861_bases
ATGAAAAATCTCTCTACGACTCAGTTTATGACCATGGCAGCAGTTATCGATTATTTGTATCAGCATCAAGATAAGCAGCC
GACGCTTGAACAGATTGCAGCGAGTGTGGGCCTGAGCTCAGGTTATGTGCAGCGCCAGTTCCAGGAATGGGTAGGTATTA
GTCCCAAAAAATTTGTTCAGTATTTGAGTTTACAGCATGCGAAATCGTTGCTTCAGCAGCAGTCGAGTATACTAGACACC
GCATTGGATTCGGGCTTATCTGGTACAGGTCGTTTGCATGATTTATTTATTCAGTTTGAGGGAATGACACCAGGTGCCTA
TAAGCAGAGAGGTGTTTCAGTCTGCTTGAATTATTCTTATGAAACCACGCCATTTGGTGAAATCTTTGTCGCCAGTACGC
CAAAAGGCATTTGTTCTATACAATTTATCCAGACAACGTCTGAGTATGAGCAGCCTCTGAATGAATTACGACAAAAATTT
GTTGCTGCTGATTTTTGTCATAGATCTGCGCCTCTACATCAGCAAGTCTCGGCATGGATTTCGGGAGACCTGACCCAGTG
TTTGCAGCAAAAGCTGCCTTTGTGTTTGCAAGGCACACCATTTCAGCTCAAGGTTTGGGAGGCTTTGCTTTGTATTCCAG
CGGGACAATTACGTAGTTATCAGCAGATTGCAGAGCAGATCGGACAGCCACAGGCGGTGCGTGCAGTTGCCAGCGCCGTA
GCTAAAAACCCAATTGCCTATTTGATTCCATGTCATCGCGTGATCCGTTCAACTGGGGCGATTGGTGAATATCATTGGCA
TCGTAGCCGTAAATTGGCTTTACTTTCATGTGAAATGGCAAAATATGGGAATCAGGGATGA

Upstream 100 bases:

>100_bases
CTTTCAAGTTGAAGGGCTTTTTTATATGTTTAGTGCAAGAATCGGGTCGAAACACGATGCTGGCTCGTCTACGCTCAACA
TAAAATGCAGGAGTTTGAGC

Downstream 100 bases:

>100_bases
CGCTGGATTTATTTGCACCACAGCCTAAAACGAACTTATTACCCTACGATGGTGACGTTGAGGATTATGGATGCTTTATT
TCTACAGCCGAAGCCGAGCA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKNLSTTQFMTMAAVIDYLYQHQDKQPTLEQIAASVGLSSGYVQRQFQEWVGISPKKFVQYLSLQHAKSLLQQQSSILDT
ALDSGLSGTGRLHDLFIQFEGMTPGAYKQRGVSVCLNYSYETTPFGEIFVASTPKGICSIQFIQTTSEYEQPLNELRQKF
VAADFCHRSAPLHQQVSAWISGDLTQCLQQKLPLCLQGTPFQLKVWEALLCIPAGQLRSYQQIAEQIGQPQAVRAVASAV
AKNPIAYLIPCHRVIRSTGAIGEYHWHRSRKLALLSCEMAKYGNQG

Sequences:

>Translated_286_residues
MKNLSTTQFMTMAAVIDYLYQHQDKQPTLEQIAASVGLSSGYVQRQFQEWVGISPKKFVQYLSLQHAKSLLQQQSSILDT
ALDSGLSGTGRLHDLFIQFEGMTPGAYKQRGVSVCLNYSYETTPFGEIFVASTPKGICSIQFIQTTSEYEQPLNELRQKF
VAADFCHRSAPLHQQVSAWISGDLTQCLQQKLPLCLQGTPFQLKVWEALLCIPAGQLRSYQQIAEQIGQPQAVRAVASAV
AKNPIAYLIPCHRVIRSTGAIGEYHWHRSRKLALLSCEMAKYGNQG
>Mature_286_residues
MKNLSTTQFMTMAAVIDYLYQHQDKQPTLEQIAASVGLSSGYVQRQFQEWVGISPKKFVQYLSLQHAKSLLQQQSSILDT
ALDSGLSGTGRLHDLFIQFEGMTPGAYKQRGVSVCLNYSYETTPFGEIFVASTPKGICSIQFIQTTSEYEQPLNELRQKF
VAADFCHRSAPLHQQVSAWISGDLTQCLQQKLPLCLQGTPFQLKVWEALLCIPAGQLRSYQQIAEQIGQPQAVRAVASAV
AKNPIAYLIPCHRVIRSTGAIGEYHWHRSRKLALLSCEMAKYGNQG

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=80, Percent_Identity=42.5, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1788542, Length=257, Percent_Identity=34.2412451361868, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1787596, Length=166, Percent_Identity=36.144578313253, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=83, Percent_Identity=51.8072289156626, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=83, Percent_Identity=51.8072289156626, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=104, Percent_Identity=39.4230769230769, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137554, Length=174, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 31921; Mature: 31921

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLSTTQFMTMAAVIDYLYQHQDKQPTLEQIAASVGLSSGYVQRQFQEWVGISPKKFVQ
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
YLSLQHAKSLLQQQSSILDTALDSGLSGTGRLHDLFIQFEGMTPGAYKQRGVSVCLNYSY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHCCCEEEEECCC
ETTPFGEIFVASTPKGICSIQFIQTTSEYEQPLNELRQKFVAADFCHRSAPLHQQVSAWI
CCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SGDLTQCLQQKLPLCLQGTPFQLKVWEALLCIPAGQLRSYQQIAEQIGQPQAVRAVASAV
HHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
AKNPIAYLIPCHRVIRSTGAIGEYHWHRSRKLALLSCEMAKYGNQG
HHCCCEEEHHHHHHHHHCCCCHHHHHHHCCCHHHEEHHHHHCCCCC
>Mature Secondary Structure
MKNLSTTQFMTMAAVIDYLYQHQDKQPTLEQIAASVGLSSGYVQRQFQEWVGISPKKFVQ
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
YLSLQHAKSLLQQQSSILDTALDSGLSGTGRLHDLFIQFEGMTPGAYKQRGVSVCLNYSY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHCCCEEEEECCC
ETTPFGEIFVASTPKGICSIQFIQTTSEYEQPLNELRQKFVAADFCHRSAPLHQQVSAWI
CCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SGDLTQCLQQKLPLCLQGTPFQLKVWEALLCIPAGQLRSYQQIAEQIGQPQAVRAVASAV
HHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
AKNPIAYLIPCHRVIRSTGAIGEYHWHRSRKLALLSCEMAKYGNQG
HHCCCEEEHHHHHHHHHCCCCHHHHHHHCCCHHHEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]