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The map label for this gene is dadX [H]

Identifier: 50083405

GI number: 50083405

Start: 116951

End: 118048

Strand: Direct

Name: dadX [H]

Synonym: ACIAD0116

Alternate gene names: 50083405

Gene position: 116951-118048 (Clockwise)

Preceding gene: 50083404

Following gene: 50083406

Centisome position: 3.25

GC content: 47.45

Gene sequence:

>1098_bases
ATGCCACGTCCGATTCAGGCTGTTATTCATCTTGAAGCACTTGCGCACAATCTTAAGATTGTGCGTGCATCTGCCCCAGA
AAGTCAAGTCTTTGCAGTAGTTAAAGCCAACGCTTATGGGCATGGAATTGAACGCGTTTATCAGGCATTTAAACAGGCAG
ATGGATTTGCACTGCTGGATATTGAAGAAGCCAAACGATTACGCGCTCTGGGCTGGACAGGGCCGATTTTATTGCTCGAA
GGCATTTTTTCGCCGCAGGATCTATTTGACTGCGTTCAATACCAGTTGAGCTTTACTGTACATCATGATGTTCAGATTGA
CTGGATCGAAGGTCATCCTGAAGACGCCCAGTTCGATATTTTTCTTAAAATGAATAGTGGCATGAACCGTCTTGGGTTTA
AACCTGTGCAGTATCGTCATGCCTGGCAGCGTTTAAATGCACTTTCCCAAGTCAGACACCTGACGCATATGATGCATTTT
TCTGATGCGGATGGTGAGCGTTTTGGCGAGTCGGGAATTACCTATCAACATGATCTGTTTGAAGACACCATTCGTGATTT
GCCAGGACCAAAATCGGTCAGCAACAGCGCTGCTATTTTACGCCATCCGTTGCACTCCGATGTGGTGCGTGGCGGAATTA
TGCTCTATGGCAGTTCACCCGATTATCCAACACATGGTCTTCATGACTGGTCACTCAAACCCACGATGAGCTTGCGTAGC
GAGATTATTGCTATCCAGATGCTGGAACCCAATGAAAGTGTGGGCTATGGCTCAAGTTTTGTGGCAGATCAACCGCTTCG
GATCGGGATTGTGGCATGTGGCTATGCAGATGGTTATCAACGAATTTCTCCGACAGGAACGCCTGTACTGGTTGATGGTA
TTGAAACGAGAACCATTGGACGTGTGAGTATGGACATGCTCGCCGTTGATCTGAGCGCAGTTCCTAAGGCAAGAATTGGT
TCGGAGGTGGTGCTTTGGGGATACTCCTCCACGGGTGTGATTTTACCGATTGATGATGTCGCGGCAGCATCTGGCACCGT
CGGTTATGAACTGATGTGTGCTGTGACCGCACGTGTTCCTTTTACAATTCAAACATAA

Upstream 100 bases:

>100_bases
GTATTGAGCCATCAGACCGAGATTAGTACCGAAGGTCTTTCACTACAGCGTTATTCAACCGCTGCTTAAGCTTTATTATT
GAGTATCAAGGAAGACACTT

Downstream 100 bases:

>100_bases
GGATAATGAGCATGTCTCAGTCTGATATTCAACGCATTAATACCACACAGGTCATGAGTTCAGTCACCATTTTTAATCAG
GTGGCTTATCTATCAGGTCA

Product: alanine racemase 2, PLP-binding, catabolic

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MPRPIQAVIHLEALAHNLKIVRASAPESQVFAVVKANAYGHGIERVYQAFKQADGFALLDIEEAKRLRALGWTGPILLLE
GIFSPQDLFDCVQYQLSFTVHHDVQIDWIEGHPEDAQFDIFLKMNSGMNRLGFKPVQYRHAWQRLNALSQVRHLTHMMHF
SDADGERFGESGITYQHDLFEDTIRDLPGPKSVSNSAAILRHPLHSDVVRGGIMLYGSSPDYPTHGLHDWSLKPTMSLRS
EIIAIQMLEPNESVGYGSSFVADQPLRIGIVACGYADGYQRISPTGTPVLVDGIETRTIGRVSMDMLAVDLSAVPKARIG
SEVVLWGYSSTGVILPIDDVAAASGTVGYELMCAVTARVPFTIQT

Sequences:

>Translated_365_residues
MPRPIQAVIHLEALAHNLKIVRASAPESQVFAVVKANAYGHGIERVYQAFKQADGFALLDIEEAKRLRALGWTGPILLLE
GIFSPQDLFDCVQYQLSFTVHHDVQIDWIEGHPEDAQFDIFLKMNSGMNRLGFKPVQYRHAWQRLNALSQVRHLTHMMHF
SDADGERFGESGITYQHDLFEDTIRDLPGPKSVSNSAAILRHPLHSDVVRGGIMLYGSSPDYPTHGLHDWSLKPTMSLRS
EIIAIQMLEPNESVGYGSSFVADQPLRIGIVACGYADGYQRISPTGTPVLVDGIETRTIGRVSMDMLAVDLSAVPKARIG
SEVVLWGYSSTGVILPIDDVAAASGTVGYELMCAVTARVPFTIQT
>Mature_364_residues
PRPIQAVIHLEALAHNLKIVRASAPESQVFAVVKANAYGHGIERVYQAFKQADGFALLDIEEAKRLRALGWTGPILLLEG
IFSPQDLFDCVQYQLSFTVHHDVQIDWIEGHPEDAQFDIFLKMNSGMNRLGFKPVQYRHAWQRLNALSQVRHLTHMMHFS
DADGERFGESGITYQHDLFEDTIRDLPGPKSVSNSAAILRHPLHSDVVRGGIMLYGSSPDYPTHGLHDWSLKPTMSLRSE
IIAIQMLEPNESVGYGSSFVADQPLRIGIVACGYADGYQRISPTGTPVLVDGIETRTIGRVSMDMLAVDLSAVPKARIGS
EVVLWGYSSTGVILPIDDVAAASGTVGYELMCAVTARVPFTIQT

Specific function: Provides the D-alanine required for cell wall biosynthesis [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=361, Percent_Identity=52.0775623268698, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1790487, Length=354, Percent_Identity=39.8305084745763, Blast_Score=247, Evalue=8e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 40251; Mature: 40120

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRPIQAVIHLEALAHNLKIVRASAPESQVFAVVKANAYGHGIERVYQAFKQADGFALLD
CCCHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEE
IEEAKRLRALGWTGPILLLEGIFSPQDLFDCVQYQLSFTVHHDVQIDWIEGHPEDAQFDI
HHHHHHHHHCCCCCHHHHEECCCCHHHHHHHHHHHEEEEEEECEEEEEECCCCCCCEEEE
FLKMNSGMNRLGFKPVQYRHAWQRLNALSQVRHLTHMMHFSDADGERFGESGITYQHDLF
EEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEHHHH
EDTIRDLPGPKSVSNSAAILRHPLHSDVVRGGIMLYGSSPDYPTHGLHDWSLKPTMSLRS
HHHHHHCCCCCCCCCCHHHHHCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHC
EIIAIQMLEPNESVGYGSSFVADQPLRIGIVACGYADGYQRISPTGTPVLVDGIETRTIG
EEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEC
RVSMDMLAVDLSAVPKARIGSEVVLWGYSSTGVILPIDDVAAASGTVGYELMCAVTARVP
HHHHHHHEEEHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCEEEEEEEEECCC
FTIQT
EEEEC
>Mature Secondary Structure 
PRPIQAVIHLEALAHNLKIVRASAPESQVFAVVKANAYGHGIERVYQAFKQADGFALLD
CCHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEE
IEEAKRLRALGWTGPILLLEGIFSPQDLFDCVQYQLSFTVHHDVQIDWIEGHPEDAQFDI
HHHHHHHHHCCCCCHHHHEECCCCHHHHHHHHHHHEEEEEEECEEEEEECCCCCCCEEEE
FLKMNSGMNRLGFKPVQYRHAWQRLNALSQVRHLTHMMHFSDADGERFGESGITYQHDLF
EEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEHHHH
EDTIRDLPGPKSVSNSAAILRHPLHSDVVRGGIMLYGSSPDYPTHGLHDWSLKPTMSLRS
HHHHHHCCCCCCCCCCHHHHHCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHC
EIIAIQMLEPNESVGYGSSFVADQPLRIGIVACGYADGYQRISPTGTPVLVDGIETRTIG
EEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEC
RVSMDMLAVDLSAVPKARIGSEVVLWGYSSTGVILPIDDVAAASGTVGYELMCAVTARVP
HHHHHHHEEEHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCEEEEEEEEECCC
FTIQT
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA