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The map label for this gene is yvfU [H]

Identifier: 49480425

GI number: 49480425

Start: 5088689

End: 5089291

Strand: Reverse

Name: yvfU [H]

Synonym: BT9727_5034

Alternate gene names: 49480425

Gene position: 5089291-5088689 (Counterclockwise)

Preceding gene: 49480426

Following gene: 49478976

Centisome position: 97.17

GC content: 38.97

Gene sequence:

>603_bases
ATGATTCGAATTATTATTGCAGAAGATCAGCGAATGCTTCGTGGTGCATTAGGGGCCCTGCTTGATCTAGAAGATGATAT
TGAAGTAATTGGGCAAGCTGCGAACGGGGAAGAGGCGCTAAAACTAATCGAATTGCTAAAACCTGATGTAAGCATTATGG
ATATTGAAATGCCAATTCAAAGTGGATTAGATGTTGCGGAAACGTTAAAGAAAGAAAAGTCAGCATGCAAAGTAATGATT
TTAACAACATTTGCGCGCCCTGGATATTTTGAACGAGCAATGAAAGCTGGCGTGCACGGATATTTATTAAAAGATAGCCC
AAGTGAAGATTTAGCTGCATCGATTCGTAATGTAATGAAAGGAAAACGAGAGATTTCTCAAGATTTAATGTTCGGCTTAT
GGCAGGAGCAAAATCCGTTATCAGATCGTGAAAAAGAAGTATTGCTACTTGCGAAAGAGGGCAAGACGGCAAATGAAATC
GCCAAGGCACTTTATCTTTCACCTGGTACAGTACGTAATTATATTTCTGAAGTATTAACGAAGCTTGATGCGAAAAATAG
AATTGAGGCAATTACAATTGCGGAAGAAAAGGGATGGATATGA

Upstream 100 bases:

>100_bases
TTATTGATGGATTCGTTGAACTAGGTACGATTAATCCAGGGACATTATTAACAGTAAAAGTTCCAGTTGTTATTCGAACA
GGAAAAGATGAGGTGAAGGC

Downstream 100 bases:

>100_bases
AAGGAAGTTTACCTATGAATGGTAAACTTCTTTTTCTTGTAATTCCGTCTTAGTTACATAGTCCTTAATTAGGGGTGTAT
GGCGTCATGTAGATTTTACT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MIRIIIAEDQRMLRGALGALLDLEDDIEVIGQAANGEEALKLIELLKPDVSIMDIEMPIQSGLDVAETLKKEKSACKVMI
LTTFARPGYFERAMKAGVHGYLLKDSPSEDLAASIRNVMKGKREISQDLMFGLWQEQNPLSDREKEVLLLAKEGKTANEI
AKALYLSPGTVRNYISEVLTKLDAKNRIEAITIAEEKGWI

Sequences:

>Translated_200_residues
MIRIIIAEDQRMLRGALGALLDLEDDIEVIGQAANGEEALKLIELLKPDVSIMDIEMPIQSGLDVAETLKKEKSACKVMI
LTTFARPGYFERAMKAGVHGYLLKDSPSEDLAASIRNVMKGKREISQDLMFGLWQEQNPLSDREKEVLLLAKEGKTANEI
AKALYLSPGTVRNYISEVLTKLDAKNRIEAITIAEEKGWI
>Mature_200_residues
MIRIIIAEDQRMLRGALGALLDLEDDIEVIGQAANGEEALKLIELLKPDVSIMDIEMPIQSGLDVAETLKKEKSACKVMI
LTTFARPGYFERAMKAGVHGYLLKDSPSEDLAASIRNVMKGKREISQDLMFGLWQEQNPLSDREKEVLLLAKEGKTANEI
AKALYLSPGTVRNYISEVLTKLDAKNRIEAITIAEEKGWI

Specific function: Member of the two-component regulatory system yvfT/yvfU [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=208, Percent_Identity=29.8076923076923, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1790102, Length=190, Percent_Identity=31.5789473684211, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=31.5789473684211, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788712, Length=197, Percent_Identity=27.9187817258883, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787473, Length=198, Percent_Identity=29.7979797979798, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786747, Length=200, Percent_Identity=23, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=33.6538461538462, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22257; Mature: 22257

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIIIAEDQRMLRGALGALLDLEDDIEVIGQAANGEEALKLIELLKPDVSIMDIEMPIQ
CEEEEEECCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCHH
SGLDVAETLKKEKSACKVMILTTFARPGYFERAMKAGVHGYLLKDSPSEDLAASIRNVMK
CCCCHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
GKREISQDLMFGLWQEQNPLSDREKEVLLLAKEGKTANEIAKALYLSPGTVRNYISEVLT
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
KLDAKNRIEAITIAEEKGWI
HHCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MIRIIIAEDQRMLRGALGALLDLEDDIEVIGQAANGEEALKLIELLKPDVSIMDIEMPIQ
CEEEEEECCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCHH
SGLDVAETLKKEKSACKVMILTTFARPGYFERAMKAGVHGYLLKDSPSEDLAASIRNVMK
CCCCHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
GKREISQDLMFGLWQEQNPLSDREKEVLLLAKEGKTANEIAKALYLSPGTVRNYISEVLT
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
KLDAKNRIEAITIAEEKGWI
HHCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]