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The map label for this gene is tcmG [H]

Identifier: 49478261

GI number: 49478261

Start: 3337081

End: 3338715

Strand: Reverse

Name: tcmG [H]

Synonym: BT9727_3271

Alternate gene names: 49478261

Gene position: 3338715-3337081 (Counterclockwise)

Preceding gene: 49478262

Following gene: 49479482

Centisome position: 63.74

GC content: 36.7

Gene sequence:

>1635_bases
GTGGAGGATAAGTATATGGAATCGAATGATGTACCAGTTTTAATTATTGGAGGGGGATTATCAGGATTAGCGTCTGCGTT
ATTCCTTGCAAAACATAATATTGAGTACTTACTTATTGAAAGGCATCTGTCTACTGCAATTCATCCAAAAGCAGGTGGAA
TCACTTTTCGTACGATGGAGTTATTTCGAGAATTAGGTTTAGAACAAAGAATTAGATCGACGGGTAAAACATTAGAAAAT
TGCCGAGGAAGAATAGCTGTTCATACAATAGCTGAGGCGAATCAGGAAGAATTGGCACAAATGAGGGCGAATCAGTATGA
AAATGATGAAAAGTTACTTCAGAAAATAGAAGAGATTAGTCCATCAAAACAAACTGCTTGTTATCAAATTACTTTAGAAG
AGATCATGTTACAAGAAGCGAGAACATTAGGCGGAGAATTATCTTTTTATCATGAATTAGTTTCTTATGAACAAAATGAG
CAAGGAGTAATCGCAACGATTCGAAATCGTGAAACAGAAAAAGAAAGTGTCATACATTGTGATTATGTAATTGCAGCAGA
TGGGGCAAAAAGTAAAATACGTGAGCAGCTAGGGATAAGTGCGGAGGGACGAGGTACAATCGGTGGCTATTATATGAATA
TTTATTTTGAAGCAGATTTAAGTGAGTTTATACAAGGAGATGCATTTGGTTTTACTATGGTACTTCATCCTGAAGTTCTT
GGTGCACTTATTCCAGTTGATAACGAAAGAAGATGGATTTACCATGTATCCTACGATCCATTAAAAGGAGAGCGACCAGA
GGATTTCACTATAGAACGTTGTAAACAAATCATTCAAACCGCAATTGGAAGCACAAATGTTGAGCCGGAAATAGTAAGTG
TTTTACCGTGGAAAGCGGCTGAAAGTACTGCTACAAAATTTCAAGATAATCGCATTTTTTTAGTTGGTGATTCTGCACAT
ATTATGCCGCCAACAGGAGGGTTTGGTTCCAATACAGGAATACAAGATGCACACAATCTAGCCTGGAAATTAGCGGCTGT
TATAAAAGGAAAGGCAAAACCAAAACTGTTAGAAACATATCATGAAGAGAGATATCCTGTTGCAAAATTAACGACAGCTT
ATGCAAGTAGTTTACTGTTTCGTGCTGCGAATAGAGAGGAAGGCAGTTTGAATAAGATGGATGGTTTAGCTGTAACTGCT
GGGTATCATTATAGTTCAGATGCAATAATAGATGACGCAGTCACTCCACATAGAATGGATATAGTACAGTTGAACGGAAG
ACCTGGAACGCGAGCACCACATTTTTGGGGGATGTATAAAGGGAAAGAAGTTTCTATATTAGACTTATTTGGTAACAATT
TTGTATTACTTACTGGAGTAGACAATAGTTCTTGGGCTGAAGCTGTATATGATGTTTCATCTAAATTAGGGATAAATATT
AATGTGTATCGAGTAGGGGGAAGCGGAGATTTTATTGCTCAAGAAAATGTTTTTCGTGAATTATATGGGATAGAAAATGA
AGGAACTGTATTAATTAGACCAGACGGCTTTATTGGATGGTGTTCGGAAAAAGCAGTGGTAAATCCAGATGTAATACTGG
AGAAGGTAATGAGTAATTTATTATGTAGTTTTTAA

Upstream 100 bases:

>100_bases
GGGGCGTAATCTATCTATATGATAATTTAAAATGAAATATAAGTCTATATCTTTGTTGTTTTTTTGATTATATTTATGTC
ACTGAGCTCAGAACAATGAT

Downstream 100 bases:

>100_bases
GTAGCTATGGAAGTATATTGTTTTATATGCTTCACTTTACTGAAAAAATAATATTCACAAATATTAATAGGTTTGGTATG
ATTAATTTCGACAAGTTAAT

Product: PheA/TfdB family polyketide hydroxylase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MEDKYMESNDVPVLIIGGGLSGLASALFLAKHNIEYLLIERHLSTAIHPKAGGITFRTMELFRELGLEQRIRSTGKTLEN
CRGRIAVHTIAEANQEELAQMRANQYENDEKLLQKIEEISPSKQTACYQITLEEIMLQEARTLGGELSFYHELVSYEQNE
QGVIATIRNRETEKESVIHCDYVIAADGAKSKIREQLGISAEGRGTIGGYYMNIYFEADLSEFIQGDAFGFTMVLHPEVL
GALIPVDNERRWIYHVSYDPLKGERPEDFTIERCKQIIQTAIGSTNVEPEIVSVLPWKAAESTATKFQDNRIFLVGDSAH
IMPPTGGFGSNTGIQDAHNLAWKLAAVIKGKAKPKLLETYHEERYPVAKLTTAYASSLLFRAANREEGSLNKMDGLAVTA
GYHYSSDAIIDDAVTPHRMDIVQLNGRPGTRAPHFWGMYKGKEVSILDLFGNNFVLLTGVDNSSWAEAVYDVSSKLGINI
NVYRVGGSGDFIAQENVFRELYGIENEGTVLIRPDGFIGWCSEKAVVNPDVILEKVMSNLLCSF

Sequences:

>Translated_544_residues
MEDKYMESNDVPVLIIGGGLSGLASALFLAKHNIEYLLIERHLSTAIHPKAGGITFRTMELFRELGLEQRIRSTGKTLEN
CRGRIAVHTIAEANQEELAQMRANQYENDEKLLQKIEEISPSKQTACYQITLEEIMLQEARTLGGELSFYHELVSYEQNE
QGVIATIRNRETEKESVIHCDYVIAADGAKSKIREQLGISAEGRGTIGGYYMNIYFEADLSEFIQGDAFGFTMVLHPEVL
GALIPVDNERRWIYHVSYDPLKGERPEDFTIERCKQIIQTAIGSTNVEPEIVSVLPWKAAESTATKFQDNRIFLVGDSAH
IMPPTGGFGSNTGIQDAHNLAWKLAAVIKGKAKPKLLETYHEERYPVAKLTTAYASSLLFRAANREEGSLNKMDGLAVTA
GYHYSSDAIIDDAVTPHRMDIVQLNGRPGTRAPHFWGMYKGKEVSILDLFGNNFVLLTGVDNSSWAEAVYDVSSKLGINI
NVYRVGGSGDFIAQENVFRELYGIENEGTVLIRPDGFIGWCSEKAVVNPDVILEKVMSNLLCSF
>Mature_544_residues
MEDKYMESNDVPVLIIGGGLSGLASALFLAKHNIEYLLIERHLSTAIHPKAGGITFRTMELFRELGLEQRIRSTGKTLEN
CRGRIAVHTIAEANQEELAQMRANQYENDEKLLQKIEEISPSKQTACYQITLEEIMLQEARTLGGELSFYHELVSYEQNE
QGVIATIRNRETEKESVIHCDYVIAADGAKSKIREQLGISAEGRGTIGGYYMNIYFEADLSEFIQGDAFGFTMVLHPEVL
GALIPVDNERRWIYHVSYDPLKGERPEDFTIERCKQIIQTAIGSTNVEPEIVSVLPWKAAESTATKFQDNRIFLVGDSAH
IMPPTGGFGSNTGIQDAHNLAWKLAAVIKGKAKPKLLETYHEERYPVAKLTTAYASSLLFRAANREEGSLNKMDGLAVTA
GYHYSSDAIIDDAVTPHRMDIVQLNGRPGTRAPHFWGMYKGKEVSILDLFGNNFVLLTGVDNSSWAEAVYDVSSKLGINI
NVYRVGGSGDFIAQENVFRELYGIENEGTVLIRPDGFIGWCSEKAVVNPDVILEKVMSNLLCSF

Specific function: Hydroxylates tetracenomycin A2 at positions C-4, C-4A, and C12A to give tetracenomycin C (TCM C). Hydroxylates as well 9- carboxymethyl-TCM B3 to yield 8-demethyl-TMC C, a side reaction [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=401, Percent_Identity=24.6882793017456, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 60546; Mature: 60546

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKYMESNDVPVLIIGGGLSGLASALFLAKHNIEYLLIERHLSTAIHPKAGGITFRTME
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCCCCEEHHHHH
LFRELGLEQRIRSTGKTLENCRGRIAVHTIAEANQEELAQMRANQYENDEKLLQKIEEIS
HHHHCCHHHHHHHHHHHHHHCCCEEEEEEEHHCCHHHHHHHHHHCCCCHHHHHHHHHHCC
PSKQTACYQITLEEIMLQEARTLGGELSFYHELVSYEQNEQGVIATIRNRETEKESVIHC
CCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEE
DYVIAADGAKSKIREQLGISAEGRGTIGGYYMNIYFEADLSEFIQGDAFGFTMVLHPEVL
EEEEECCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCHHHHHCCCCCEEEEEECHHHH
GALIPVDNERRWIYHVSYDPLKGERPEDFTIERCKQIIQTAIGSTNVEPEIVSVLPWKAA
HEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
ESTATKFQDNRIFLVGDSAHIMPPTGGFGSNTGIQDAHNLAWKLAAVIKGKAKPKLLETY
CHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
HEERYPVAKLTTAYASSLLFRAANREEGSLNKMDGLAVTAGYHYSSDAIIDDAVTPHRMD
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEECCCCEECCCCCCCEEE
IVQLNGRPGTRAPHFWGMYKGKEVSILDLFGNNFVLLTGVDNSSWAEAVYDVSSKLGINI
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCEEE
NVYRVGGSGDFIAQENVFRELYGIENEGTVLIRPDGFIGWCSEKAVVNPDVILEKVMSNL
EEEEECCCCCCEEHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHH
LCSF
HCCC
>Mature Secondary Structure
MEDKYMESNDVPVLIIGGGLSGLASALFLAKHNIEYLLIERHLSTAIHPKAGGITFRTME
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCCCCEEHHHHH
LFRELGLEQRIRSTGKTLENCRGRIAVHTIAEANQEELAQMRANQYENDEKLLQKIEEIS
HHHHCCHHHHHHHHHHHHHHCCCEEEEEEEHHCCHHHHHHHHHHCCCCHHHHHHHHHHCC
PSKQTACYQITLEEIMLQEARTLGGELSFYHELVSYEQNEQGVIATIRNRETEKESVIHC
CCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEE
DYVIAADGAKSKIREQLGISAEGRGTIGGYYMNIYFEADLSEFIQGDAFGFTMVLHPEVL
EEEEECCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCHHHHHCCCCCEEEEEECHHHH
GALIPVDNERRWIYHVSYDPLKGERPEDFTIERCKQIIQTAIGSTNVEPEIVSVLPWKAA
HEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
ESTATKFQDNRIFLVGDSAHIMPPTGGFGSNTGIQDAHNLAWKLAAVIKGKAKPKLLETY
CHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
HEERYPVAKLTTAYASSLLFRAANREEGSLNKMDGLAVTAGYHYSSDAIIDDAVTPHRMD
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEECCCCEECCCCCCCEEE
IVQLNGRPGTRAPHFWGMYKGKEVSILDLFGNNFVLLTGVDNSSWAEAVYDVSSKLGINI
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCEEE
NVYRVGGSGDFIAQENVFRELYGIENEGTVLIRPDGFIGWCSEKAVVNPDVILEKVMSNL
EEEEECCCCCCEEHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHH
LCSF
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]