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The map label for this gene is trmE

Identifier: 49476318

GI number: 49476318

Start: 1927797

End: 1929104

Strand: Reverse

Name: trmE

Synonym: BH16690

Alternate gene names: 49476318

Gene position: 1929104-1927797 (Counterclockwise)

Preceding gene: 49476319

Following gene: 49476317

Centisome position: 99.9

GC content: 37.69

Gene sequence:

>1308_bases
GTGGATACAATCTTTGCTGTTTCGAGTGGATTGTTGCCTTCAGGGGTTGCTGTCATTCGGCTTTCAGGTTCTCATGTTGT
GCATGTTGTTACAACGCTTTGTGGTTGTTTACCTAAAGCGCGGTTTATGCATTATGGAAATCTTACAGCTCGTGATGGTA
GTTTTTTAGATTCTGCTTTAACTGTTTTTTTTCCTGCTCCTCATAGTTTTACAGGGGAAGATTGTGCGGAATTTCATTTG
CATGGAGGAAAAGCTGTTGTTAATCGTTTTCTTGATGAATTGTCCACATTTAAAGGGTGTCGCATTGCAGAGCCAGGTGA
ATTTTCACGCCGTGCTTTTATGGAGGGAAAATTAGATCTTGTTCAAGCAGAAGGTCTTGCTGATTTAATAGAAGCAGAGA
CGGAAAGTCAGCGACGTTTAGCAGTTATGGGTACAAGTGGGCGTTTAACGACGCTTTATCGTGATTGGCGTCATAAACTT
ATGAAAATTCGTGCTTTTATTGAAGCGGAACTTGATTTTTCTGATGAAGCTGATATCCCCAACACGGTATCTGATAAAGT
TTGGAAAGATGTTGAAAATCTTTGCATTTCTCTTCGAGAACATATTGCTGAAGGGGAGCGTGCGAGTATTTTACGTGATG
GGTTTAAAATTGTCATTGTAGGTGCTCCAAATTCTGGAAAATCAAGCATTATGAATCGTTTGGCTGGAAAACCTGTTGCT
ATTGTGACAGAAGAAGCAGGAACAACACGTGATGCTTTAGAAGTAAGACTTGTTTTGGGTGGTTTACCGGTTTTTTTAAT
AGATACTGCTGGTTTTAGAGAAACAGATAACAAAATAGAACAGTTAGGAATAGAAGTTGCAAAGCAGCATGTTAGGGACG
CTGACTTGGTTATTTTAGTTTATGATATGCAAAATCCTAAGGAAGTTTATTTACCAGAAACGTCTGCTGAAATATGGCGT
GTAGGCAATAAATTTGATCTTTATGAAGAGAATAAAGAGCCATGGTTAATACAATTTTCGGCATTAACGGGTTTAAATTT
TGATCATTTTATGAAGGAACTTGAATCGTTTTGTTTACGTCGTGTTTCTGAGATTGGAAATCTTTTTCCTGCGCGTAAGA
GACAACTTCAATTATTAAAAGAAGCTGTTAAGGAGATTGAAAATTCTGTGAATTATGACTCTCTTGATCTCAGTTTACGT
GCAGAACATCTTCGTCGTGCCAGTGATTTTCTTGGAAAAATTACCGGAGATATAGATGTTGAGGATTTGCTTGATATTAT
TTTTTCAGAGTTTTGTATTGGCAAATAA

Upstream 100 bases:

>100_bases
AATTTTTGATTGACAAGTTAAAACAAACAAAAAACAATAGTGAATTTTTTGATTCAATGAATACGTAGAAGTTTTAAGTT
TTTTGATTTAGGATAATCCT

Downstream 100 bases:

>100_bases
TGATTCACGTGAAACTTGAAAACAATTTTTTGTGGAATGAATTAAATGCAATTATATGATGTTGTTGTTATTGGAGGTGG
TCATGCTGGCTGCGAAGCAG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MDTIFAVSSGLLPSGVAVIRLSGSHVVHVVTTLCGCLPKARFMHYGNLTARDGSFLDSALTVFFPAPHSFTGEDCAEFHL
HGGKAVVNRFLDELSTFKGCRIAEPGEFSRRAFMEGKLDLVQAEGLADLIEAETESQRRLAVMGTSGRLTTLYRDWRHKL
MKIRAFIEAELDFSDEADIPNTVSDKVWKDVENLCISLREHIAEGERASILRDGFKIVIVGAPNSGKSSIMNRLAGKPVA
IVTEEAGTTRDALEVRLVLGGLPVFLIDTAGFRETDNKIEQLGIEVAKQHVRDADLVILVYDMQNPKEVYLPETSAEIWR
VGNKFDLYEENKEPWLIQFSALTGLNFDHFMKELESFCLRRVSEIGNLFPARKRQLQLLKEAVKEIENSVNYDSLDLSLR
AEHLRRASDFLGKITGDIDVEDLLDIIFSEFCIGK

Sequences:

>Translated_435_residues
MDTIFAVSSGLLPSGVAVIRLSGSHVVHVVTTLCGCLPKARFMHYGNLTARDGSFLDSALTVFFPAPHSFTGEDCAEFHL
HGGKAVVNRFLDELSTFKGCRIAEPGEFSRRAFMEGKLDLVQAEGLADLIEAETESQRRLAVMGTSGRLTTLYRDWRHKL
MKIRAFIEAELDFSDEADIPNTVSDKVWKDVENLCISLREHIAEGERASILRDGFKIVIVGAPNSGKSSIMNRLAGKPVA
IVTEEAGTTRDALEVRLVLGGLPVFLIDTAGFRETDNKIEQLGIEVAKQHVRDADLVILVYDMQNPKEVYLPETSAEIWR
VGNKFDLYEENKEPWLIQFSALTGLNFDHFMKELESFCLRRVSEIGNLFPARKRQLQLLKEAVKEIENSVNYDSLDLSLR
AEHLRRASDFLGKITGDIDVEDLLDIIFSEFCIGK
>Mature_435_residues
MDTIFAVSSGLLPSGVAVIRLSGSHVVHVVTTLCGCLPKARFMHYGNLTARDGSFLDSALTVFFPAPHSFTGEDCAEFHL
HGGKAVVNRFLDELSTFKGCRIAEPGEFSRRAFMEGKLDLVQAEGLADLIEAETESQRRLAVMGTSGRLTTLYRDWRHKL
MKIRAFIEAELDFSDEADIPNTVSDKVWKDVENLCISLREHIAEGERASILRDGFKIVIVGAPNSGKSSIMNRLAGKPVA
IVTEEAGTTRDALEVRLVLGGLPVFLIDTAGFRETDNKIEQLGIEVAKQHVRDADLVILVYDMQNPKEVYLPETSAEIWR
VGNKFDLYEENKEPWLIQFSALTGLNFDHFMKELESFCLRRVSEIGNLFPARKRQLQLLKEAVKEIENSVNYDSLDLSLR
AEHLRRASDFLGKITGDIDVEDLLDIIFSEFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=463, Percent_Identity=39.9568034557235, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI193082993, Length=447, Percent_Identity=40.9395973154362, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI193082988, Length=495, Percent_Identity=37.5757575757576, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=38.9867841409692, Blast_Score=274, Evalue=9e-75,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=37.3626373626374, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=449, Percent_Identity=34.2984409799555, Blast_Score=217, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6323665, Length=494, Percent_Identity=35.4251012145749, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI45550813, Length=470, Percent_Identity=35.7446808510638, Blast_Score=276, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BARHE (Q6G1K8)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034359.1
- HSSP:   P25522
- ProteinModelPortal:   Q6G1K8
- SMR:   Q6G1K8
- GeneID:   2865210
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH16690
- NMPDR:   fig|283166.1.peg.1487
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- PhylomeDB:   Q6G1K8
- ProtClustDB:   PRK05291
- BioCyc:   BHEN283166:BH16690-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48640; Mature: 48640

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: BINDING 20-20 BINDING 77-77 BINDING 117-117 BINDING 435-435

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTIFAVSSGLLPSGVAVIRLSGSHVVHVVTTLCGCLPKARFMHYGNLTARDGSFLDSAL
CCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHCCE
TVFFPAPHSFTGEDCAEFHLHGGKAVVNRFLDELSTFKGCRIAEPGEFSRRAFMEGKLDL
EEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHE
VQAEGLADLIEAETESQRRLAVMGTSGRLTTLYRDWRHKLMKIRAFIEAELDFSDEADIP
EHHCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NTVSDKVWKDVENLCISLREHIAEGERASILRDGFKIVIVGAPNSGKSSIMNRLAGKPVA
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCEE
IVTEEAGTTRDALEVRLVLGGLPVFLIDTAGFRETDNKIEQLGIEVAKQHVRDADLVILV
EEECCCCCCHHHHHEEHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
YDMQNPKEVYLPETSAEIWRVGNKFDLYEENKEPWLIQFSALTGLNFDHFMKELESFCLR
EECCCCCEEECCCCCHHHHHCCCEECCCCCCCCCEEEEEECHHCCCHHHHHHHHHHHHHH
RVSEIGNLFPARKRQLQLLKEAVKEIENSVNYDSLDLSLRAEHLRRASDFLGKITGDIDV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCH
EDLLDIIFSEFCIGK
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTIFAVSSGLLPSGVAVIRLSGSHVVHVVTTLCGCLPKARFMHYGNLTARDGSFLDSAL
CCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHCCE
TVFFPAPHSFTGEDCAEFHLHGGKAVVNRFLDELSTFKGCRIAEPGEFSRRAFMEGKLDL
EEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHE
VQAEGLADLIEAETESQRRLAVMGTSGRLTTLYRDWRHKLMKIRAFIEAELDFSDEADIP
EHHCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NTVSDKVWKDVENLCISLREHIAEGERASILRDGFKIVIVGAPNSGKSSIMNRLAGKPVA
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCEE
IVTEEAGTTRDALEVRLVLGGLPVFLIDTAGFRETDNKIEQLGIEVAKQHVRDADLVILV
EEECCCCCCHHHHHEEHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
YDMQNPKEVYLPETSAEIWRVGNKFDLYEENKEPWLIQFSALTGLNFDHFMKELESFCLR
EECCCCCEEECCCCCHHHHHCCCEECCCCCCCCCEEEEEECHHCCCHHHHHHHHHHHHHH
RVSEIGNLFPARKRQLQLLKEAVKEIENSVNYDSLDLSLRAEHLRRASDFLGKITGDIDV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCH
EDLLDIIFSEFCIGK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA