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The map label for this gene is gidA2

Identifier: 49476317

GI number: 49476317

Start: 1925883

End: 1927751

Strand: Reverse

Name: gidA2

Synonym: BH16680

Alternate gene names: 49476317

Gene position: 1927751-1925883 (Counterclockwise)

Preceding gene: 49476318

Following gene: 49476316

Centisome position: 99.83

GC content: 37.72

Gene sequence:

>1869_bases
ATGCAATTATATGATGTTGTTGTTATTGGAGGTGGTCATGCTGGCTGCGAAGCAGCTTATGCATCAGCGCGTGCTGGAGC
ACAGACAGCACTCATTACACATAAAATATCAGCACTAGGAACAATGTCGTGTAATCCGGCTATTGGAGGGCTTGGAAAAG
GACATCTGGTTCGTGAAATAGATGCTTTTGATGGGCTCATGGGACGAGCAGCAGATGCAGCTGGAATTCAATTTCGACTT
CTTAATCGACGAAAAGGACCAGCAGTAAGAGGTCCGCGCGCGCAAGCTGACCGACAACTCTATAAAGAAGCAATTCAAAA
GCTTTTACAAAAACAAGATAATCTTATTCTCATTGAAGATGAAGTTATTGATCTGATTGTTAAAGATAAGAGTATTTCAG
GTGTTATATTAAAAAAACAAGGAATACTCCCTTCTGGTGCTGTTGTTTTGACAACAGGTACCTTTTTAAATGGTTTTATT
CATATTGGTGATAAAACATGGGCTGCTGGACGTATGGGAGAACAGTCAAGTATAAAACTTGCTGAACGTTTAAAAAGTTA
TGGCATAAATCTTGGTCGATTAAAAACAGGAACTCCTGCTCGTCTTAGCAAAAAAACAATCCGTTGGGAATGTCTTCCTA
AACAGCAAGCTGACGAAAATCCTGTACCTTTTTCTCTTTTGACTGAAAAAATTGAACAGCCTCAAATTGAATGTGCCATA
ACACGTACAAATGCACAAACACATCAGATTATTCGTGAAAATATTCATCGCTCTGCTTTATATTCAGGAAATATTGAAGG
ATTAGGACCACGTTATTGTCCTTCAATTGAAGATAAGATTGTTAAATTTGGAGAACGTGATGGGCATCAGATTTTCCTAG
AACCAGAAGGATTAAATGATGATACTATTTATCCAAATGGTCTTTCTACTTCTCTTCCTGAAGATGTACAAGTTTCTTTA
TTGAAAACAATTGAAGGATTGGAAAATGTTACAATTCTACAATCTGGTTACGCTATTGAATATGATTTTGTTAATCCACA
ACAGTTAACTAAAACTTTGGAGTTACGTTCTTTACCAGGATTATTTTTAGCTGGCCAAATTAATGGGACAACAGGATATG
AAGAAGCCGCAGCACAAGGTCTTTTGGCTGGACTAAATGCCGCACGTAAGGTAGGTGGATTAGATGAAATACTTATAAGT
CGTTCTACTGCTTATATTGGTGTTATGGTAGATGATTTAGTCTCGCGTGGGGTTTGTGAACCTTATAGAATGTTTACATC
ACGTGCTGAATTTCGTTTATCTCTGCGATCTGATAATGCTGATGCTCGTTTGACACCTTTAGCACAACAATTAGGTATTG
TAAGTAAATTACGTTGGGAGTGCTATCAGAAAAAACAACAGCGTCTTGATCAAGCGCGATCACTATGCCAAGAGCTTTTT
CTAACGCCTAATCAAGCATCTGCTCATGGGTTGCAAGTGAATCATGATGGAATTCGGCGTTCAGCTTATGATCTTCTTGC
TTATCCTCATATGAGTATAGAGCGTCTTTCACATATTTGGCCACAGTTACAATCCATTGATTCTAAAACAGTTGAATCTG
TAGAAATTGAAGCACAATATGCAGTTTATTTGGAAAAACAAGCACAAGATATCCTTGCTCTTCAACGAGATGAGCGTTTA
GAAATTCCTTCTTCTCTTGATGTTCAGGCAATTTCTGGTCTTTCTAATGAGCTCAAAACAAAAATTCAAAAAATATCGCC
ACGTTCAATTGCTGATGCACAAAAAATTGATGGTATGACGCCAGCAGCATTATCACTTATTATCACTTATATTCAACGTC
AAAGACGTGAAAAGGCTAAATCAGCTTAA

Upstream 100 bases:

>100_bases
AGATGTTGAGGATTTGCTTGATATTATTTTTTCAGAGTTTTGTATTGGCAAATAATGATTCACGTGAAACTTGAAAACAA
TTTTTTGTGGAATGAATTAA

Downstream 100 bases:

>100_bases
TGGATTTTTCTATAGAACAAAAATATCAAGAGCTTTTAAAAATAATTTCTTCTGTTTCACGTGAAACGATGCAGGATTTA
ATGCATTTTGAATCTTTGAT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MQLYDVVVIGGGHAGCEAAYASARAGAQTALITHKISALGTMSCNPAIGGLGKGHLVREIDAFDGLMGRAADAAGIQFRL
LNRRKGPAVRGPRAQADRQLYKEAIQKLLQKQDNLILIEDEVIDLIVKDKSISGVILKKQGILPSGAVVLTTGTFLNGFI
HIGDKTWAAGRMGEQSSIKLAERLKSYGINLGRLKTGTPARLSKKTIRWECLPKQQADENPVPFSLLTEKIEQPQIECAI
TRTNAQTHQIIRENIHRSALYSGNIEGLGPRYCPSIEDKIVKFGERDGHQIFLEPEGLNDDTIYPNGLSTSLPEDVQVSL
LKTIEGLENVTILQSGYAIEYDFVNPQQLTKTLELRSLPGLFLAGQINGTTGYEEAAAQGLLAGLNAARKVGGLDEILIS
RSTAYIGVMVDDLVSRGVCEPYRMFTSRAEFRLSLRSDNADARLTPLAQQLGIVSKLRWECYQKKQQRLDQARSLCQELF
LTPNQASAHGLQVNHDGIRRSAYDLLAYPHMSIERLSHIWPQLQSIDSKTVESVEIEAQYAVYLEKQAQDILALQRDERL
EIPSSLDVQAISGLSNELKTKIQKISPRSIADAQKIDGMTPAALSLIITYIQRQRREKAKSA

Sequences:

>Translated_622_residues
MQLYDVVVIGGGHAGCEAAYASARAGAQTALITHKISALGTMSCNPAIGGLGKGHLVREIDAFDGLMGRAADAAGIQFRL
LNRRKGPAVRGPRAQADRQLYKEAIQKLLQKQDNLILIEDEVIDLIVKDKSISGVILKKQGILPSGAVVLTTGTFLNGFI
HIGDKTWAAGRMGEQSSIKLAERLKSYGINLGRLKTGTPARLSKKTIRWECLPKQQADENPVPFSLLTEKIEQPQIECAI
TRTNAQTHQIIRENIHRSALYSGNIEGLGPRYCPSIEDKIVKFGERDGHQIFLEPEGLNDDTIYPNGLSTSLPEDVQVSL
LKTIEGLENVTILQSGYAIEYDFVNPQQLTKTLELRSLPGLFLAGQINGTTGYEEAAAQGLLAGLNAARKVGGLDEILIS
RSTAYIGVMVDDLVSRGVCEPYRMFTSRAEFRLSLRSDNADARLTPLAQQLGIVSKLRWECYQKKQQRLDQARSLCQELF
LTPNQASAHGLQVNHDGIRRSAYDLLAYPHMSIERLSHIWPQLQSIDSKTVESVEIEAQYAVYLEKQAQDILALQRDERL
EIPSSLDVQAISGLSNELKTKIQKISPRSIADAQKIDGMTPAALSLIITYIQRQRREKAKSA
>Mature_622_residues
MQLYDVVVIGGGHAGCEAAYASARAGAQTALITHKISALGTMSCNPAIGGLGKGHLVREIDAFDGLMGRAADAAGIQFRL
LNRRKGPAVRGPRAQADRQLYKEAIQKLLQKQDNLILIEDEVIDLIVKDKSISGVILKKQGILPSGAVVLTTGTFLNGFI
HIGDKTWAAGRMGEQSSIKLAERLKSYGINLGRLKTGTPARLSKKTIRWECLPKQQADENPVPFSLLTEKIEQPQIECAI
TRTNAQTHQIIRENIHRSALYSGNIEGLGPRYCPSIEDKIVKFGERDGHQIFLEPEGLNDDTIYPNGLSTSLPEDVQVSL
LKTIEGLENVTILQSGYAIEYDFVNPQQLTKTLELRSLPGLFLAGQINGTTGYEEAAAQGLLAGLNAARKVGGLDEILIS
RSTAYIGVMVDDLVSRGVCEPYRMFTSRAEFRLSLRSDNADARLTPLAQQLGIVSKLRWECYQKKQQRLDQARSLCQELF
LTPNQASAHGLQVNHDGIRRSAYDLLAYPHMSIERLSHIWPQLQSIDSKTVESVEIEAQYAVYLEKQAQDILALQRDERL
EIPSSLDVQAISGLSNELKTKIQKISPRSIADAQKIDGMTPAALSLIITYIQRQRREKAKSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=644, Percent_Identity=42.8571428571429, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI19882217, Length=669, Percent_Identity=41.2556053811659, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI183227703, Length=684, Percent_Identity=40.3508771929825, Blast_Score=474, Evalue=1e-133,
Organism=Escherichia coli, GI2367273, Length=617, Percent_Identity=49.7568881685575, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17534255, Length=619, Percent_Identity=42.1647819063005, Blast_Score=496, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6321202, Length=623, Percent_Identity=47.8330658105939, Blast_Score=565, Evalue=1e-162,
Organism=Drosophila melanogaster, GI24658174, Length=635, Percent_Identity=45.6692913385827, Blast_Score=535, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_BARHE (Q6G1K9)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034358.1
- ProteinModelPortal:   Q6G1K9
- SMR:   Q6G1K9
- GeneID:   2864738
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH16680
- NMPDR:   fig|283166.1.peg.1486
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   Q6G1K9
- ProtClustDB:   PRK05192
- BioCyc:   BHEN283166:BH16680-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 68483; Mature: 68483

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2; PS00189 LIPOYL

Important sites: BINDING 122-122 BINDING 177-177 BINDING 366-366

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLYDVVVIGGGHAGCEAAYASARAGAQTALITHKISALGTMSCNPAIGGLGKGHLVREI
CCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
DAFDGLMGRAADAAGIQFRLLNRRKGPAVRGPRAQADRQLYKEAIQKLLQKQDNLILIED
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEH
EVIDLIVKDKSISGVILKKQGILPSGAVVLTTGTFLNGFIHIGDKTWAAGRMGEQSSIKL
HEEEEEEECCCCCEEEEEECCCCCCCCEEEEECHHHCCEEEECCCCCCCCCCCCCHHHHH
AERLKSYGINLGRLKTGTPARLSKKTIRWECLPKQQADENPVPFSLLTEKIEQPQIECAI
HHHHHHHCCCCCEECCCCCCHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEE
TRTNAQTHQIIRENIHRSALYSGNIEGLGPRYCPSIEDKIVKFGERDGHQIFLEPEGLND
EECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCC
DTIYPNGLSTSLPEDVQVSLLKTIEGLENVTILQSGYAIEYDFVNPQQLTKTLELRSLPG
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEECCCHHHHHHHHHHHCCCC
LFLAGQINGTTGYEEAAAQGLLAGLNAARKVGGLDEILISRSTAYIGVMVDDLVSRGVCE
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHCCCCC
PYRMFTSRAEFRLSLRSDNADARLTPLAQQLGIVSKLRWECYQKKQQRLDQARSLCQELF
HHHHHHCCHHEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LTPNQASAHGLQVNHDGIRRSAYDLLAYPHMSIERLSHIWPQLQSIDSKTVESVEIEAQY
CCCCCCCCCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHEEEEEHH
AVYLEKQAQDILALQRDERLEIPSSLDVQAISGLSNELKTKIQKISPRSIADAQKIDGMT
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCHHCCHHHHCCCC
PAALSLIITYIQRQRREKAKSA
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQLYDVVVIGGGHAGCEAAYASARAGAQTALITHKISALGTMSCNPAIGGLGKGHLVREI
CCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
DAFDGLMGRAADAAGIQFRLLNRRKGPAVRGPRAQADRQLYKEAIQKLLQKQDNLILIED
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEH
EVIDLIVKDKSISGVILKKQGILPSGAVVLTTGTFLNGFIHIGDKTWAAGRMGEQSSIKL
HEEEEEEECCCCCEEEEEECCCCCCCCEEEEECHHHCCEEEECCCCCCCCCCCCCHHHHH
AERLKSYGINLGRLKTGTPARLSKKTIRWECLPKQQADENPVPFSLLTEKIEQPQIECAI
HHHHHHHCCCCCEECCCCCCHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEE
TRTNAQTHQIIRENIHRSALYSGNIEGLGPRYCPSIEDKIVKFGERDGHQIFLEPEGLND
EECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCC
DTIYPNGLSTSLPEDVQVSLLKTIEGLENVTILQSGYAIEYDFVNPQQLTKTLELRSLPG
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEECCCHHHHHHHHHHHCCCC
LFLAGQINGTTGYEEAAAQGLLAGLNAARKVGGLDEILISRSTAYIGVMVDDLVSRGVCE
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHCCCCC
PYRMFTSRAEFRLSLRSDNADARLTPLAQQLGIVSKLRWECYQKKQQRLDQARSLCQELF
HHHHHHCCHHEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LTPNQASAHGLQVNHDGIRRSAYDLLAYPHMSIERLSHIWPQLQSIDSKTVESVEIEAQY
CCCCCCCCCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHEEEEEHH
AVYLEKQAQDILALQRDERLEIPSSLDVQAISGLSNELKTKIQKISPRSIADAQKIDGMT
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCHHCCHHHHCCCC
PAALSLIITYIQRQRREKAKSA
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA