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The map label for this gene is rimO

Identifier: 49475606

GI number: 49475606

Start: 946040

End: 947353

Strand: Reverse

Name: rimO

Synonym: BH08410

Alternate gene names: 49475606

Gene position: 947353-946040 (Counterclockwise)

Preceding gene: 49475607

Following gene: 49475601

Centisome position: 49.06

GC content: 38.96

Gene sequence:

>1314_bases
ATGGCAGCACCTCGTATTAGTTTTGTTTCTCTTGGATGCCCCAAAGCACTTGTAGATTCTGAACGAATTATTACAAGCCT
TCGATCCGAAGGGTATGAAATTTCGCGCAAACATCAAGGTGCTGATCTCGTTATTGTGAATACCTGTGGTTTTCTTGATT
CTGCCCGAAATGAATCACTCGCCAATATTGATGAAGCCCTTAAAAAAAATGGAAAAGTTATTGTAACTGGGTGTCTTGGT
GCTGATCCTGATGTTATTCGCCAAGTATATCCTAATGTATTGGCCATTACAGGACCACAAGCCTATGAAAGTGTTATACA
AGCAGTTCATACGGCAATTCCTCCAGTTCATGATCCTTTTATAGATTTAGTTCCCCCTCAAGGGATTCGCTTAACACCTC
GCCATTATGCTTATTTAAAGATTTCTGAAGGATGCTCTAATCGGTGTAGCTTTTGTATTATCCCTACTCTGCGCGGTAAT
CTTATGTCACGTCCTATCAGTGATGTTCTTCGTGAAGCTGAAAAACTTGTACAAGCAGGGGTAAAAGAGCTTTTGGTTAT
TTCACAAGATACAAGTGCTTACGGCATTGATCTTAAATATCTAGAAACGCCTTGGAAAGATCGCACAGTAAAAACGAAGT
TTTTCGATCTTTGTCGCGAATTAGGCAATATGGGCATTTGGATACGCATGCACTATGTCTATCCTTATCCCCATGTCGAT
GACGTTATTGAATTTATGGCGGAAAAGAAAATTCTCCCTTATCTGGATATCCCTTTTCAGCATGCATCACCAACTGTTTT
GCGCAACATGAAACGCCCTGCTCTTGTAAAAAAAATAAATCGTAGAATTGAAAAATGGCGAATAATTTGTCCAGATCTCA
CTTTGCGATCCACATTTATTGTTGGCTTTCCAGGTGAAACTCATGAAGATTTTAACATACTTCTTGAATGGTTAGACGAA
GTAAAAATTGAACGCGCTGGCTGCTTTAAATATGAAGCAGTAAAAGGTGCTGTTGCAAATGATTTGGGATTAGAAAGTAT
TCCTGAAGAAGTGAAGGAAAACCGCTGGCATCGCTTTATGGCAAAACAACAAAAAATTTCTACTCATCTTTTAAAGAAAA
AAGTTGGAAAAAGACTTCAAGTTCTTATCGATGAAAGTCAAGGCAAAATAGCTAAAGGTCGTAGTCAATATGATGCTCCA
GAAATAGACGGCGTTGTGCATATATCGTCACGACGCCCTTTACGTGTTGGTGAATTTGTCACTGTTAAAATCGAACGATC
AGATGCTTATGATCTCTATGGTATCGCAATTTGA

Upstream 100 bases:

>100_bases
GTTGCGTATGTAAAAAAATCAAGAGCATTTTTGTTTTTAATGAAATAAAAGTAGCATAAAATTCGATTGTTATAAATGTT
ACAAAGTTGCAGGAATTATC

Downstream 100 bases:

>100_bases
AATTATCAATTTATGAAGATCGCGGTCAATATGATGAAAATAAACATTATTGCGTATATATCGTCACGACTGTCGTTTAC
ATGAATATTTAATCTTTAGA

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MAAPRISFVSLGCPKALVDSERIITSLRSEGYEISRKHQGADLVIVNTCGFLDSARNESLANIDEALKKNGKVIVTGCLG
ADPDVIRQVYPNVLAITGPQAYESVIQAVHTAIPPVHDPFIDLVPPQGIRLTPRHYAYLKISEGCSNRCSFCIIPTLRGN
LMSRPISDVLREAEKLVQAGVKELLVISQDTSAYGIDLKYLETPWKDRTVKTKFFDLCRELGNMGIWIRMHYVYPYPHVD
DVIEFMAEKKILPYLDIPFQHASPTVLRNMKRPALVKKINRRIEKWRIICPDLTLRSTFIVGFPGETHEDFNILLEWLDE
VKIERAGCFKYEAVKGAVANDLGLESIPEEVKENRWHRFMAKQQKISTHLLKKKVGKRLQVLIDESQGKIAKGRSQYDAP
EIDGVVHISSRRPLRVGEFVTVKIERSDAYDLYGIAI

Sequences:

>Translated_437_residues
MAAPRISFVSLGCPKALVDSERIITSLRSEGYEISRKHQGADLVIVNTCGFLDSARNESLANIDEALKKNGKVIVTGCLG
ADPDVIRQVYPNVLAITGPQAYESVIQAVHTAIPPVHDPFIDLVPPQGIRLTPRHYAYLKISEGCSNRCSFCIIPTLRGN
LMSRPISDVLREAEKLVQAGVKELLVISQDTSAYGIDLKYLETPWKDRTVKTKFFDLCRELGNMGIWIRMHYVYPYPHVD
DVIEFMAEKKILPYLDIPFQHASPTVLRNMKRPALVKKINRRIEKWRIICPDLTLRSTFIVGFPGETHEDFNILLEWLDE
VKIERAGCFKYEAVKGAVANDLGLESIPEEVKENRWHRFMAKQQKISTHLLKKKVGKRLQVLIDESQGKIAKGRSQYDAP
EIDGVVHISSRRPLRVGEFVTVKIERSDAYDLYGIAI
>Mature_436_residues
AAPRISFVSLGCPKALVDSERIITSLRSEGYEISRKHQGADLVIVNTCGFLDSARNESLANIDEALKKNGKVIVTGCLGA
DPDVIRQVYPNVLAITGPQAYESVIQAVHTAIPPVHDPFIDLVPPQGIRLTPRHYAYLKISEGCSNRCSFCIIPTLRGNL
MSRPISDVLREAEKLVQAGVKELLVISQDTSAYGIDLKYLETPWKDRTVKTKFFDLCRELGNMGIWIRMHYVYPYPHVDD
VIEFMAEKKILPYLDIPFQHASPTVLRNMKRPALVKKINRRIEKWRIICPDLTLRSTFIVGFPGETHEDFNILLEWLDEV
KIERAGCFKYEAVKGAVANDLGLESIPEEVKENRWHRFMAKQQKISTHLLKKKVGKRLQVLIDESQGKIAKGRSQYDAPE
IDGVVHISSRRPLRVGEFVTVKIERSDAYDLYGIAI

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=396, Percent_Identity=27.5252525252525, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=24.5283018867925, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=24.5283018867925, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1787057, Length=432, Percent_Identity=61.3425925925926, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=27.1714922048998, Blast_Score=141, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=23.6170212765957, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71996771, Length=300, Percent_Identity=27, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21356207, Length=482, Percent_Identity=24.0663900414938, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI19922432, Length=320, Percent_Identity=26.5625, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_BARHE (Q6G5E0)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033647.1
- ProteinModelPortal:   Q6G5E0
- SMR:   Q6G5E0
- GeneID:   2865850
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH08410
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q6G5E0
- ProtClustDB:   PRK14862
- BioCyc:   BHEN283166:BH08410-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49449; Mature: 49318

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPRISFVSLGCPKALVDSERIITSLRSEGYEISRKHQGADLVIVNTCGFLDSARNESL
CCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEHHHCCCCCEEEEECCCCHHHHCCCHH
ANIDEALKKNGKVIVTGCLGADPDVIRQVYPNVLAITGPQAYESVIQAVHTAIPPVHDPF
HHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCC
IDLVPPQGIRLTPRHYAYLKISEGCSNRCSFCIIPTLRGNLMSRPISDVLREAEKLVQAG
CCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHHHHH
VKELLVISQDTSAYGIDLKYLETPWKDRTVKTKFFDLCRELGNMGIWIRMHYVYPYPHVD
HHHEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHH
DVIEFMAEKKILPYLDIPFQHASPTVLRNMKRPALVKKINRRIEKWRIICPDLTLRSTFI
HHHHHHHHCCCCCEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHEEECCCCEEEEEEE
VGFPGETHEDFNILLEWLDEVKIERAGCFKYEAVKGAVANDLGLESIPEEVKENRWHRFM
EECCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHCCHHHHCCCHHHHHHHHHHHHHHHHH
AKQQKISTHLLKKKVGKRLQVLIDESQGKIAKGRSQYDAPEIDGVVHISSRRPLRVGEFV
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCEEECCEE
TVKIERSDAYDLYGIAI
EEEEECCCCCCEEEEEC
>Mature Secondary Structure 
AAPRISFVSLGCPKALVDSERIITSLRSEGYEISRKHQGADLVIVNTCGFLDSARNESL
CCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEHHHCCCCCEEEEECCCCHHHHCCCHH
ANIDEALKKNGKVIVTGCLGADPDVIRQVYPNVLAITGPQAYESVIQAVHTAIPPVHDPF
HHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCC
IDLVPPQGIRLTPRHYAYLKISEGCSNRCSFCIIPTLRGNLMSRPISDVLREAEKLVQAG
CCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHHHHH
VKELLVISQDTSAYGIDLKYLETPWKDRTVKTKFFDLCRELGNMGIWIRMHYVYPYPHVD
HHHEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHH
DVIEFMAEKKILPYLDIPFQHASPTVLRNMKRPALVKKINRRIEKWRIICPDLTLRSTFI
HHHHHHHHCCCCCEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHEEECCCCEEEEEEE
VGFPGETHEDFNILLEWLDEVKIERAGCFKYEAVKGAVANDLGLESIPEEVKENRWHRFM
EECCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHCCHHHHCCCHHHHHHHHHHHHHHHHH
AKQQKISTHLLKKKVGKRLQVLIDESQGKIAKGRSQYDAPEIDGVVHISSRRPLRVGEFV
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCEEECCEE
TVKIERSDAYDLYGIAI
EEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA