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The map label for this gene is lexA

Identifier: 49475607

GI number: 49475607

Start: 947584

End: 948288

Strand: Reverse

Name: lexA

Synonym: BH08420

Alternate gene names: 49475607

Gene position: 948288-947584 (Counterclockwise)

Preceding gene: 49475615

Following gene: 49475606

Centisome position: 49.11

GC content: 36.45

Gene sequence:

>705_bases
ATGCTGACTTGTAAACAATATGAGCTACTTTTGTTTATTCATAATCATATGAAAGAAACAGGCGTCCCACCCTCCTTTGA
TGAAATGAAAACTGCATTGGAGCTTACTTCAAAATCTGGCATCCATAGACTTATTACAGCCTTAGAAGAGCGAGGCTTTA
TACGCCGTTTGCCAAATCGCGCCCGTGCAGTGGAAGTTATCCGACTTCCCGAAAAAATAACATTTAACCTTTCTTCAGCA
CGTAAAATTTCCCCTAGTGTAATAGAGAATAATAAAAGAAAAATATCAAAAAACTCTGACAATTTTGATTTGGAAGAAAA
AAAAAACATTATTATTCCCATTATGGGCCGCATTGCTGCTGCTGTACCTATTTCCGCTATACAGCAACAAATAAATACGC
TTTGTTTGCCACAAGATATGATTAGCTTGGGTGAACATTATGCCCTAGAGGTCAAAGATGATTCTATGATTGAGGCTGGA
ATTCTTGATAAAGATATTATAATTGTTAGACGTCAAAATACTGCAACATCAGGCGAAATTATTATTGCACTTATCGATAA
AGAAGAAGTAACCTTTAAACGTTATCGACGCAAAGGAAACTCTATTACATTGGAAGCTGCAAATCCTCACTATGAAACAC
GTATATACAGACCAGAGCGTGTGCAGATACAAGGTAAGCTTATCGGACTTATTCGCAAATATTAA

Upstream 100 bases:

>100_bases
AAATAGATATTATTCTTTTATAAAGATAAAAATTTTAGAATAATTACTTAAAATTATATTTTTTATTCTCTCTTTTTTAA
TTCTTTTGATTGGAACTTTA

Downstream 100 bases:

>100_bases
AAAACAAAAAAGTTACTGAAAAAAACCCTCTATTCAATGAATAGAGGGTTTAAGTATTTTTTTAGCATGTTATCTGCTAC
GCAGATCCATAAAATTTTGT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MLTCKQYELLLFIHNHMKETGVPPSFDEMKTALELTSKSGIHRLITALEERGFIRRLPNRARAVEVIRLPEKITFNLSSA
RKISPSVIENNKRKISKNSDNFDLEEKKNIIIPIMGRIAAAVPISAIQQQINTLCLPQDMISLGEHYALEVKDDSMIEAG
ILDKDIIIVRRQNTATSGEIIIALIDKEEVTFKRYRRKGNSITLEAANPHYETRIYRPERVQIQGKLIGLIRKY

Sequences:

>Translated_234_residues
MLTCKQYELLLFIHNHMKETGVPPSFDEMKTALELTSKSGIHRLITALEERGFIRRLPNRARAVEVIRLPEKITFNLSSA
RKISPSVIENNKRKISKNSDNFDLEEKKNIIIPIMGRIAAAVPISAIQQQINTLCLPQDMISLGEHYALEVKDDSMIEAG
ILDKDIIIVRRQNTATSGEIIIALIDKEEVTFKRYRRKGNSITLEAANPHYETRIYRPERVQIQGKLIGLIRKY
>Mature_234_residues
MLTCKQYELLLFIHNHMKETGVPPSFDEMKTALELTSKSGIHRLITALEERGFIRRLPNRARAVEVIRLPEKITFNLSSA
RKISPSVIENNKRKISKNSDNFDLEEKKNIIIPIMGRIAAAVPISAIQQQINTLCLPQDMISLGEHYALEVKDDSMIEAG
ILDKDIIIVRRQNTATSGEIIIALIDKEEVTFKRYRRKGNSITLEAANPHYETRIYRPERVQIQGKLIGLIRKY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=233, Percent_Identity=24.0343347639485, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_BARHE (Q6G5D9)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033648.1
- ProteinModelPortal:   Q6G5D9
- SMR:   Q6G5D9
- MEROPS:   S24.001
- GeneID:   2865581
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH08420
- NMPDR:   fig|283166.1.peg.776
- HOGENOM:   HBG679610
- OMA:   DDEFHIR
- PhylomeDB:   Q6G5D9
- ProtClustDB:   PRK00215
- BioCyc:   BHEN283166:BH08420-MONOMER
- BRENDA:   3.4.21.88
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 26777; Mature: 26777

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: ACT_SITE 155-155 ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTCKQYELLLFIHNHMKETGVPPSFDEMKTALELTSKSGIHRLITALEERGFIRRLPNR
CCCCCHHHHEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCH
ARAVEVIRLPEKITFNLSSARKISPSVIENNKRKISKNSDNFDLEEKKNIIIPIMGRIAA
HHEEEEEECCCEEEEECCCCCCCCHHHHCCCCHHCCCCCCCCCCCCCCCEEEEEHHHHHH
AVPISAIQQQINTLCLPQDMISLGEHYALEVKDDSMIEAGILDKDIIIVRRQNTATSGEI
HCCHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCE
IIALIDKEEVTFKRYRRKGNSITLEAANPHYETRIYRPERVQIQGKLIGLIRKY
EEEEECCCHHHHHHHHCCCCEEEEEECCCCCEEEEECCCEEEECHHHEEHHHCC
>Mature Secondary Structure
MLTCKQYELLLFIHNHMKETGVPPSFDEMKTALELTSKSGIHRLITALEERGFIRRLPNR
CCCCCHHHHEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCH
ARAVEVIRLPEKITFNLSSARKISPSVIENNKRKISKNSDNFDLEEKKNIIIPIMGRIAA
HHEEEEEECCCEEEEECCCCCCCCHHHHCCCCHHCCCCCCCCCCCCCCCEEEEEHHHHHH
AVPISAIQQQINTLCLPQDMISLGEHYALEVKDDSMIEAGILDKDIIIVRRQNTATSGEI
HCCHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCE
IIALIDKEEVTFKRYRRKGNSITLEAANPHYETRIYRPERVQIQGKLIGLIRKY
EEEEECCCHHHHHHHHCCCCEEEEEECCCCCEEEEECCCEEEECHHHEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA