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The map label for this gene is thiC

Identifier: 49475286

GI number: 49475286

Start: 569308

End: 571140

Strand: Reverse

Name: thiC

Synonym: BH04910

Alternate gene names: 49475286

Gene position: 571140-569308 (Counterclockwise)

Preceding gene: 49475288

Following gene: 49475285

Centisome position: 29.58

GC content: 38.9

Gene sequence:

>1833_bases
ATGCAGAAAAATATTCCATCAATCAGTTGCAGTCCCTTTCCCGCCTCACGCAAAATTTATCAACAAAGTCCTCTCTTTTC
TGATGTTCAGGTACCACTGCGTGAAATTTCATTGACAGATGGCAGTGCTGAAAAATCTTTAAATATCTATGATACGTCTG
GCCCTTACACTGACAACGATATAATCATTGATATTACAAATGGCTTGCCCGCAATTAGTTTACCTTGGCTTTCTAAGAGA
GCTGATACTGAACCTTATCCTGCACGACCAGTCAAACCTGAAGATAATGGATTGCTTTATGATAAAACGCCTCCTCCGGC
CTTTAAACAAAAGCGGCCTATTTTGAGGGCAAAAAACGGTAAAGCAATTACACAAATGGCTTACGCACGTGCAGGCATTA
TTACCGCAGAAATGGAATATGTGGCTATACGAGAAAATGAAGGATTAACAGTAAAAGATCAGCCAAAAGCGCAACCCGAT
AAAATATGCAATGGATCAATACCAGAAATTTATACTGCGGAATTTGTTCGCAATGAAATTGCCTGCGGACGTGCTATTAT
TCCTCATAACATCAATCATCCAGAATGTGAGCCAATGATTATTGGACGAAATTTTCGTGTTAAAATTAATGCCAATATTG
GAAATTCAGCAGTTACTTCATCTATGGCAGAAGAAGTTGATAAAATGGTTTGGGCTATTCGTTGGGGAGCAGATACAGTT
ATGGATCTTTCAACAGGCCGAAATATCCATAATATTCGTGAGTGGATTATTAGGAATTCTCCAGTTCCCATCGGAACTGT
TCCACTTTATCAAGCACTTGAAAAAGTACAGGGTATTGCTGAAAACTTAACATGGGATATTTTTCGTGATACTCTTATTG
AACAAGCAGAACAGGGTGTTGATTATTTTACTATCCATGCAGGATTAAGATTACCTTTCATTCCTCTAACCATTGATCGC
ATAACAGGTATTGTTTCACGGGGCGGTTCTATTATGGCAAAATGGTGCCTCCATCATCACAAAGAAAGTTTTCTCTATGA
ACATTTTGATGAAATTTGTGACATTGCACGCACGTATGATATCTCTCTTTCATTAGGAGATGGATTGCGTCCTGGCTCTA
TTGCCGATGCTAATGATGAAGCTCAATTTGCTGAACTTAAAACTTTGGGGGAATTAACAAAAGTTGCCTGGGCAAAAGAT
GTACAGGTTATGATTGAAGGACCTGGGCATGTGCCAATGCACAAAATTAAAGAAAATATGGAACAACAATTAGATCTCTG
TCATGAAGCGCCTTTTTATACTTTGGGACCTCTTACAACTGATATTGCACCTGGATATGACCATATTACATCAGCAATTG
GTGCTGCTATGATTGGATGGTTTGGAACTGCCATGCTTTGTTACGTAACACCTAAAGAACATTTAGGACTTCCCGATAAG
AATGATGTCAAAACTGGTGTAATCACTTACAAAATCGCTGCTCATGCTGCTGATCTTGCAAAAGGTTTACCAAGAGCACA
ACTACGCGATAACGCTCTCTCACGTGCACGATTTGATTTTCGATGGTATGATCAATTTAACCTTTCTCTTGATCCAGAAA
CAGCCTGTGCTTTTCATGATGAAACAATGCCTAAGGAAGCTCATAAATTGGTGCATTTTTGCTCCATGTGTGGACCAAAA
TTTTGTTCTATGCGTATTTCCCATGATATTCGTGACACAGTACACAGAAAAATAAAAGATGAAGGTATGGTCGCCATGGC
GGAAAAATATCAAAAGAATGGTGATCTCTATATAGACGTTAGTCCTCCTCAAAAGAAGATAGTAAACCATTGA

Upstream 100 bases:

>100_bases
ATATATGCGTTCTTTTTAAGCGTATGTATTGATGATTTGAATACATCGCCGTTAACATCGCTATCTGTTTTTGAATGATG
AATCTATAAGGAAAAAGTTT

Downstream 100 bases:

>100_bases
AAAACATTCTTATTAAAGGTGCAGGTGTAGGGGGATTAACAGTCGCCTTTATGTTACAACAAAAAGGTGTTTCTGTTACC
ATTTCAGCACCCCCTCATTC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MQKNIPSISCSPFPASRKIYQQSPLFSDVQVPLREISLTDGSAEKSLNIYDTSGPYTDNDIIIDITNGLPAISLPWLSKR
ADTEPYPARPVKPEDNGLLYDKTPPPAFKQKRPILRAKNGKAITQMAYARAGIITAEMEYVAIRENEGLTVKDQPKAQPD
KICNGSIPEIYTAEFVRNEIACGRAIIPHNINHPECEPMIIGRNFRVKINANIGNSAVTSSMAEEVDKMVWAIRWGADTV
MDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVQGIAENLTWDIFRDTLIEQAEQGVDYFTIHAGLRLPFIPLTIDR
ITGIVSRGGSIMAKWCLHHHKESFLYEHFDEICDIARTYDISLSLGDGLRPGSIADANDEAQFAELKTLGELTKVAWAKD
VQVMIEGPGHVPMHKIKENMEQQLDLCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDK
NDVKTGVITYKIAAHAADLAKGLPRAQLRDNALSRARFDFRWYDQFNLSLDPETACAFHDETMPKEAHKLVHFCSMCGPK
FCSMRISHDIRDTVHRKIKDEGMVAMAEKYQKNGDLYIDVSPPQKKIVNH

Sequences:

>Translated_610_residues
MQKNIPSISCSPFPASRKIYQQSPLFSDVQVPLREISLTDGSAEKSLNIYDTSGPYTDNDIIIDITNGLPAISLPWLSKR
ADTEPYPARPVKPEDNGLLYDKTPPPAFKQKRPILRAKNGKAITQMAYARAGIITAEMEYVAIRENEGLTVKDQPKAQPD
KICNGSIPEIYTAEFVRNEIACGRAIIPHNINHPECEPMIIGRNFRVKINANIGNSAVTSSMAEEVDKMVWAIRWGADTV
MDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVQGIAENLTWDIFRDTLIEQAEQGVDYFTIHAGLRLPFIPLTIDR
ITGIVSRGGSIMAKWCLHHHKESFLYEHFDEICDIARTYDISLSLGDGLRPGSIADANDEAQFAELKTLGELTKVAWAKD
VQVMIEGPGHVPMHKIKENMEQQLDLCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDK
NDVKTGVITYKIAAHAADLAKGLPRAQLRDNALSRARFDFRWYDQFNLSLDPETACAFHDETMPKEAHKLVHFCSMCGPK
FCSMRISHDIRDTVHRKIKDEGMVAMAEKYQKNGDLYIDVSPPQKKIVNH
>Mature_610_residues
MQKNIPSISCSPFPASRKIYQQSPLFSDVQVPLREISLTDGSAEKSLNIYDTSGPYTDNDIIIDITNGLPAISLPWLSKR
ADTEPYPARPVKPEDNGLLYDKTPPPAFKQKRPILRAKNGKAITQMAYARAGIITAEMEYVAIRENEGLTVKDQPKAQPD
KICNGSIPEIYTAEFVRNEIACGRAIIPHNINHPECEPMIIGRNFRVKINANIGNSAVTSSMAEEVDKMVWAIRWGADTV
MDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVQGIAENLTWDIFRDTLIEQAEQGVDYFTIHAGLRLPFIPLTIDR
ITGIVSRGGSIMAKWCLHHHKESFLYEHFDEICDIARTYDISLSLGDGLRPGSIADANDEAQFAELKTLGELTKVAWAKD
VQVMIEGPGHVPMHKIKENMEQQLDLCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDK
NDVKTGVITYKIAAHAADLAKGLPRAQLRDNALSRARFDFRWYDQFNLSLDPETACAFHDETMPKEAHKLVHFCSMCGPK
FCSMRISHDIRDTVHRKIKDEGMVAMAEKYQKNGDLYIDVSPPQKKIVNH

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=612, Percent_Identity=63.2352941176471, Blast_Score=795, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_BARHE (Q6G477)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033327.1
- GeneID:   2865211
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH04910
- NMPDR:   fig|283166.1.peg.455
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q6G477
- ProtClustDB:   PRK09284
- BioCyc:   BHEN283166:BH04910-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 68245; Mature: 68245

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: BINDING 212-212 BINDING 241-241 BINDING 270-270 BINDING 306-306 BINDING 406-406 BINDING 433-433

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKNIPSISCSPFPASRKIYQQSPLFSDVQVPLREISLTDGSAEKSLNIYDTSGPYTDND
CCCCCCCCCCCCCCCHHHHHHCCCCCCHHHCCHHHEEECCCCCCCCEEEEECCCCCCCCC
IIIDITNGLPAISLPWLSKRADTEPYPARPVKPEDNGLLYDKTPPPAFKQKRPILRAKNG
EEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCEECCCC
KAITQMAYARAGIITAEMEYVAIRENEGLTVKDQPKAQPDKICNGSIPEIYTAEFVRNEI
CHHHHHHHHHHCEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ACGRAIIPHNINHPECEPMIIGRNFRVKINANIGNSAVTSSMAEEVDKMVWAIRWGADTV
HCCCEECCCCCCCCCCCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCE
MDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVQGIAENLTWDIFRDTLIEQAEQGV
EECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
DYFTIHAGLRLPFIPLTIDRITGIVSRGGSIMAKWCLHHHKESFLYEHFDEICDIARTYD
CEEEEECCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ISLSLGDGLRPGSIADANDEAQFAELKTLGELTKVAWAKDVQVMIEGPGHVPMHKIKENM
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHH
EQQLDLCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDK
HHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCC
NDVKTGVITYKIAAHAADLAKGLPRAQLRDNALSRARFDFRWYDQFNLSLDPETACAFHD
CCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCEECCCHHEEECC
ETMPKEAHKLVHFCSMCGPKFCSMRISHDIRDTVHRKIKDEGMVAMAEKYQKNGDLYIDV
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEC
SPPQKKIVNH
CCCHHHHCCC
>Mature Secondary Structure
MQKNIPSISCSPFPASRKIYQQSPLFSDVQVPLREISLTDGSAEKSLNIYDTSGPYTDND
CCCCCCCCCCCCCCCHHHHHHCCCCCCHHHCCHHHEEECCCCCCCCEEEEECCCCCCCCC
IIIDITNGLPAISLPWLSKRADTEPYPARPVKPEDNGLLYDKTPPPAFKQKRPILRAKNG
EEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCEECCCC
KAITQMAYARAGIITAEMEYVAIRENEGLTVKDQPKAQPDKICNGSIPEIYTAEFVRNEI
CHHHHHHHHHHCEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ACGRAIIPHNINHPECEPMIIGRNFRVKINANIGNSAVTSSMAEEVDKMVWAIRWGADTV
HCCCEECCCCCCCCCCCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCE
MDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVQGIAENLTWDIFRDTLIEQAEQGV
EECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
DYFTIHAGLRLPFIPLTIDRITGIVSRGGSIMAKWCLHHHKESFLYEHFDEICDIARTYD
CEEEEECCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ISLSLGDGLRPGSIADANDEAQFAELKTLGELTKVAWAKDVQVMIEGPGHVPMHKIKENM
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHH
EQQLDLCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDK
HHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCC
NDVKTGVITYKIAAHAADLAKGLPRAQLRDNALSRARFDFRWYDQFNLSLDPETACAFHD
CCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCEECCCHHEEECC
ETMPKEAHKLVHFCSMCGPKFCSMRISHDIRDTVHRKIKDEGMVAMAEKYQKNGDLYIDV
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEC
SPPQKKIVNH
CCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA