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The map label for this gene is ispB [H]

Identifier: 49475216

GI number: 49475216

Start: 481232

End: 482302

Strand: Reverse

Name: ispB [H]

Synonym: BH04180

Alternate gene names: 49475216

Gene position: 482302-481232 (Counterclockwise)

Preceding gene: 49475224

Following gene: 49475215

Centisome position: 24.98

GC content: 38.47

Gene sequence:

>1071_bases
ATGCTATGGATAAAATTCATATATGTCCATTACAATACTCTAGGAGTAAAAATATTGCGTGCTGCTATAAAATCGGATCA
AATAAAAAATAATCCAATTTCTCTCCAATCCCTCGTCAATCTCACCAAAGATGATATGGAACACGTAAACCAATGCATCT
TCTCTATGGCAAAATCAGACGTTGAAATAATTCCTGAAATTTCCAACCATCTTATCTCTTCAGGTGGAAAACGATTGCGT
CCAATGCTTACATTAGCTTCTGCTCGTATGTTTGGTTATCAAAATGATGGACATATAAAACTCGCAACAGCAGTTGAATT
TATGCATACGGCAACCTTATTACATGATGACGTAATCGATGAAAGCAATTTACGACGCGGAAAATCTACTGCAAGAATGA
TTTGGGGAAATCAAGCAAGCATACTTGTTGGTGATTTCCTATTAGGACAAGCTTTTAAAATGATGGTCGATGTTGGTTCT
ATAGAGGCACTCTCTGTCTTAGCAAATGCTGCAGCAATTATTGCAGAGGGAGAAGTTATGCAACTTTCGGCCACAAAAAA
TATAGAAACCAGCATTTCAGATTATCTCAAAATCATCAATGCAAAAACGGCTGCACTTTTTTCAGCGGCCGCTGAAGTTG
GACCAATTATTGCTGGTTATGGACATGAAGAGCGTCTTGCATTGCGCGAATATGGAACATTTTTAGGATTAGCCTTCCAA
TTGATTGACGATGCACTTGACTATGGTGGAACTGCTAAATATTTGGGGAAAAATATAGGCGATGATTTTAGAGAAGGCAA
AATCACAATGCCTGTTATTCTTGCATATGCCCGCAGCAACGCCGTAGAAAAAACATTCTGGAAACAAGCGCTTGAAAATG
GCAATAGCAATGATGAAACCTTCGCACATGCACAACACTTAATAGAAAAATATGATAGCCTCACAGACACAATAGAACAA
GCGCGTAGCTATGGAAAAAACGCAACTCATGCTCTCGCTGCAATAAGCGAAAGCCCCATTCATAATGCATTAATTGAAAC
TGTTGACTTTTGTATTGCCCGTGTAAACTGA

Upstream 100 bases:

>100_bases
CGTGATTATCCAAGCATAAAAAACATTCTTTGCGAATACTACCCAAAACTTGCATTTTCAAATGCGCTATCAAAGCTTGA
GTCAAAGCAAAACCCTCTTT

Downstream 100 bases:

>100_bases
TTATTTGCAATAATTACAATATTTTTCAATAAAGTAACCGGATATGGTTTATAAGTACAGTCAATTGATTATCTTAAGAA
GGATTAGCTCTATGCGCAGT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLWIKFIYVHYNTLGVKILRAAIKSDQIKNNPISLQSLVNLTKDDMEHVNQCIFSMAKSDVEIIPEISNHLISSGGKRLR
PMLTLASARMFGYQNDGHIKLATAVEFMHTATLLHDDVIDESNLRRGKSTARMIWGNQASILVGDFLLGQAFKMMVDVGS
IEALSVLANAAAIIAEGEVMQLSATKNIETSISDYLKIINAKTAALFSAAAEVGPIIAGYGHEERLALREYGTFLGLAFQ
LIDDALDYGGTAKYLGKNIGDDFREGKITMPVILAYARSNAVEKTFWKQALENGNSNDETFAHAQHLIEKYDSLTDTIEQ
ARSYGKNATHALAAISESPIHNALIETVDFCIARVN

Sequences:

>Translated_356_residues
MLWIKFIYVHYNTLGVKILRAAIKSDQIKNNPISLQSLVNLTKDDMEHVNQCIFSMAKSDVEIIPEISNHLISSGGKRLR
PMLTLASARMFGYQNDGHIKLATAVEFMHTATLLHDDVIDESNLRRGKSTARMIWGNQASILVGDFLLGQAFKMMVDVGS
IEALSVLANAAAIIAEGEVMQLSATKNIETSISDYLKIINAKTAALFSAAAEVGPIIAGYGHEERLALREYGTFLGLAFQ
LIDDALDYGGTAKYLGKNIGDDFREGKITMPVILAYARSNAVEKTFWKQALENGNSNDETFAHAQHLIEKYDSLTDTIEQ
ARSYGKNATHALAAISESPIHNALIETVDFCIARVN
>Mature_356_residues
MLWIKFIYVHYNTLGVKILRAAIKSDQIKNNPISLQSLVNLTKDDMEHVNQCIFSMAKSDVEIIPEISNHLISSGGKRLR
PMLTLASARMFGYQNDGHIKLATAVEFMHTATLLHDDVIDESNLRRGKSTARMIWGNQASILVGDFLLGQAFKMMVDVGS
IEALSVLANAAAIIAEGEVMQLSATKNIETSISDYLKIINAKTAALFSAAAEVGPIIAGYGHEERLALREYGTFLGLAFQ
LIDDALDYGGTAKYLGKNIGDDFREGKITMPVILAYARSNAVEKTFWKQALENGNSNDETFAHAQHLIEKYDSLTDTIEQ
ARSYGKNATHALAAISESPIHNALIETVDFCIARVN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=296, Percent_Identity=27.7027027027027, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI4758430, Length=234, Percent_Identity=23.9316239316239, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI83700220, Length=234, Percent_Identity=23.9316239316239, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789578, Length=329, Percent_Identity=41.0334346504559, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1786623, Length=256, Percent_Identity=33.203125, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17505681, Length=294, Percent_Identity=30.6122448979592, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=240, Percent_Identity=30.4166666666667, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6325188, Length=224, Percent_Identity=24.5535714285714, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24651612, Length=324, Percent_Identity=28.7037037037037, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI281365769, Length=292, Percent_Identity=24.6575342465753, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24660002, Length=292, Percent_Identity=24.6575342465753, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 39126; Mature: 39126

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWIKFIYVHYNTLGVKILRAAIKSDQIKNNPISLQSLVNLTKDDMEHVNQCIFSMAKSD
CEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VEIIPEISNHLISSGGKRLRPMLTLASARMFGYQNDGHIKLATAVEFMHTATLLHDDVID
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCC
ESNLRRGKSTARMIWGNQASILVGDFLLGQAFKMMVDVGSIEALSVLANAAAIIAEGEVM
CHHHHCCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCEE
QLSATKNIETSISDYLKIINAKTAALFSAAAEVGPIIAGYGHEERLALREYGTFLGLAFQ
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHH
LIDDALDYGGTAKYLGKNIGDDFREGKITMPVILAYARSNAVEKTFWKQALENGNSNDET
HHHHHHCCCCHHHHHHCCCCCHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHH
FAHAQHLIEKYDSLTDTIEQARSYGKNATHALAAISESPIHNALIETVDFCIARVN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLWIKFIYVHYNTLGVKILRAAIKSDQIKNNPISLQSLVNLTKDDMEHVNQCIFSMAKSD
CEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VEIIPEISNHLISSGGKRLRPMLTLASARMFGYQNDGHIKLATAVEFMHTATLLHDDVID
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCC
ESNLRRGKSTARMIWGNQASILVGDFLLGQAFKMMVDVGSIEALSVLANAAAIIAEGEVM
CHHHHCCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCEE
QLSATKNIETSISDYLKIINAKTAALFSAAAEVGPIIAGYGHEERLALREYGTFLGLAFQ
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHH
LIDDALDYGGTAKYLGKNIGDDFREGKITMPVILAYARSNAVEKTFWKQALENGNSNDET
HHHHHHCCCCHHHHHHCCCCCHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHH
FAHAQHLIEKYDSLTDTIEQARSYGKNATHALAAISESPIHNALIETVDFCIARVN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]