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The map label for this gene is purD [H]

Identifier: 49475224

GI number: 49475224

Start: 491381

End: 492664

Strand: Reverse

Name: purD [H]

Synonym: BH04270

Alternate gene names: 49475224

Gene position: 492664-491381 (Counterclockwise)

Preceding gene: 49475226

Following gene: 49475216

Centisome position: 25.51

GC content: 41.2

Gene sequence:

>1284_bases
ATGAACATTCTTCTTATCGGCTCAGGCGGACGGGAACATGCTTTGGCATGGAAAATAGCAGCATCTCCAATGCTTACAAA
ATTATATTGCGCTCCTGGAAATCCTGCAACCATAGAATTGGGTGAAAATATTGATTTGGACATTAATGACCACCATCTTG
TTATTGATTTTTGCAAAACACATTCTATTGACCTTGTGATTGTTGGACCAGAAGCACCACTGGTTGCGGGTATAACAGAC
TCTCTTAATAACGCTAATATCTGTGTATTCGGACCTACTCAAAAAGCTGCTCAGCTAGAAGGTTCAAAAGCTTTTACAAA
GGATTTATGCCGTAAGAATAACATTCCTACAGGAACTTACCAATGTTTTAATGATGCTGAAAAAGCTAAAGCCTACATTC
GTCAACAAGGTGTTCCCATTGTCATTAAAGCCGATGGCTTAGCTGCTGGAAAAGGTGTTGTCGTTGCTATGACAATGGAA
GAGGCATTTAACGCTGTTGATGCCTGCTTCAAAAGTGCTTTTGGTGATGCAGGAAACAAAATTGTTGTAGAATCCTTTCT
TGAAGGTGAAGAAGCAAGCTTTTTCTGCCTTTGTGATGGTAAAATTGCTATACCTTTTGGATCTGCTCAAGATCACAAAC
GTGTCGGTGATGGTGATACTGGAGCAAATACCGGCGGCATGGGAGCTTATTCACCAGCTCCCATCATGACCCAAGAAATG
GTTAATCGTACCCTCAACGAAATTGTTGAACCGGTTCTACATAGCTTGAATCAAATGGGATCTCCCTTTAAAGGTATTCT
CTTCGTTGGATTGATGATAACGAAAAAAGGACCCGAATTAATTGAATTTAATGTACGATTCGGTGATCCAGAATGCCAAG
TTTTAATGATGCGTCTACAGAATGATATTCTCCCGATTCTGCTTGCAGCAGCGCGAGGAAATCTTGAAAACACCTCTCTT
CAATGGTCTAAAAAAACTGCTTTAACCGTTGTTATGGCTGCGAAAGGTTATCCAGACTCTCCTCAAAAAGGCACTGTTAT
CCGTAATGTTGATAAGGTGAACGCTCTTCCTGATGTGAAAGTTTTTCAAGCCGCTACAGTATTACACAATGGAGAACTCA
TTGCCAATGGTGGACGTGTTTTAAATATAACAGCGACAGGAGAAACTATTCTGCAGGCACAAAAACGCGCTTATGAAGCT
GTTGATTTTATTGACTGGCCAGAAGGCTTTGTACGCCGCGATATTGGATGGCGAGCTGTTGCGCGAGAAAATTCAATCCT
TTGA

Upstream 100 bases:

>100_bases
TTTTTTCATCAAATGGCTTCACCTTTAGTCTTGCGATAGAAGCATTTCACTCTTATGACAAGAAAACTGATTTCATCATC
TTCTGTATTAGGAGTAAGTA

Downstream 100 bases:

>100_bases
ATATTCTTTTTTGATAATTCGATAATTTCACCTAGAGATCATGGAGCATAAATCTGCTATATATTCTGTATGACTCATGA
TTTTGGAAAAAATATTGAAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNILLIGSGGREHALAWKIAASPMLTKLYCAPGNPATIELGENIDLDINDHHLVIDFCKTHSIDLVIVGPEAPLVAGITD
SLNNANICVFGPTQKAAQLEGSKAFTKDLCRKNNIPTGTYQCFNDAEKAKAYIRQQGVPIVIKADGLAAGKGVVVAMTME
EAFNAVDACFKSAFGDAGNKIVVESFLEGEEASFFCLCDGKIAIPFGSAQDHKRVGDGDTGANTGGMGAYSPAPIMTQEM
VNRTLNEIVEPVLHSLNQMGSPFKGILFVGLMITKKGPELIEFNVRFGDPECQVLMMRLQNDILPILLAAARGNLENTSL
QWSKKTALTVVMAAKGYPDSPQKGTVIRNVDKVNALPDVKVFQAATVLHNGELIANGGRVLNITATGETILQAQKRAYEA
VDFIDWPEGFVRRDIGWRAVARENSIL

Sequences:

>Translated_427_residues
MNILLIGSGGREHALAWKIAASPMLTKLYCAPGNPATIELGENIDLDINDHHLVIDFCKTHSIDLVIVGPEAPLVAGITD
SLNNANICVFGPTQKAAQLEGSKAFTKDLCRKNNIPTGTYQCFNDAEKAKAYIRQQGVPIVIKADGLAAGKGVVVAMTME
EAFNAVDACFKSAFGDAGNKIVVESFLEGEEASFFCLCDGKIAIPFGSAQDHKRVGDGDTGANTGGMGAYSPAPIMTQEM
VNRTLNEIVEPVLHSLNQMGSPFKGILFVGLMITKKGPELIEFNVRFGDPECQVLMMRLQNDILPILLAAARGNLENTSL
QWSKKTALTVVMAAKGYPDSPQKGTVIRNVDKVNALPDVKVFQAATVLHNGELIANGGRVLNITATGETILQAQKRAYEA
VDFIDWPEGFVRRDIGWRAVARENSIL
>Mature_427_residues
MNILLIGSGGREHALAWKIAASPMLTKLYCAPGNPATIELGENIDLDINDHHLVIDFCKTHSIDLVIVGPEAPLVAGITD
SLNNANICVFGPTQKAAQLEGSKAFTKDLCRKNNIPTGTYQCFNDAEKAKAYIRQQGVPIVIKADGLAAGKGVVVAMTME
EAFNAVDACFKSAFGDAGNKIVVESFLEGEEASFFCLCDGKIAIPFGSAQDHKRVGDGDTGANTGGMGAYSPAPIMTQEM
VNRTLNEIVEPVLHSLNQMGSPFKGILFVGLMITKKGPELIEFNVRFGDPECQVLMMRLQNDILPILLAAARGNLENTSL
QWSKKTALTVVMAAKGYPDSPQKGTVIRNVDKVNALPDVKVFQAATVLHNGELIANGGRVLNITATGETILQAQKRAYEA
VDFIDWPEGFVRRDIGWRAVARENSIL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=44.4705882352941, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=44.4705882352941, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=44.4705882352941, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=44.4705882352941, Blast_Score=364, Evalue=1e-101,
Organism=Escherichia coli, GI1790438, Length=430, Percent_Identity=51.1627906976744, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=38.3529411764706, Blast_Score=278, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=41.3870246085011, Blast_Score=328, Evalue=1e-90,
Organism=Drosophila melanogaster, GI62471619, Length=414, Percent_Identity=41.0628019323672, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24582400, Length=414, Percent_Identity=41.0628019323672, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45957; Mature: 45957

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLIGSGGREHALAWKIAASPMLTKLYCAPGNPATIELGENIDLDINDHHLVIDFCKT
CEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCEEEEECCCEEEEECCCEEEEEEECC
HSIDLVIVGPEAPLVAGITDSLNNANICVFGPTQKAAQLEGSKAFTKDLCRKNNIPTGTY
CCEEEEEECCCCCEEEECCCCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHCCCCCCCHH
QCFNDAEKAKAYIRQQGVPIVIKADGLAAGKGVVVAMTMEEAFNAVDACFKSAFGDAGNK
HHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCE
IVVESFLEGEEASFFCLCDGKIAIPFGSAQDHKRVGDGDTGANTGGMGAYSPAPIMTQEM
EEEEHHCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VNRTLNEIVEPVLHSLNQMGSPFKGILFVGLMITKKGPELIEFNVRFGDPECQVLMMRLQ
HHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHH
NDILPILLAAARGNLENTSLQWSKKTALTVVMAAKGYPDSPQKGTVIRNVDKVNALPDVK
CCHHHHHHHHHCCCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCEEECHHHHCCCCCCH
VFQAATVLHNGELIANGGRVLNITATGETILQAQKRAYEAVDFIDWPEGFVRRDIGWRAV
HHHHHHEEECCEEEECCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEE
ARENSIL
CCCCCCC
>Mature Secondary Structure
MNILLIGSGGREHALAWKIAASPMLTKLYCAPGNPATIELGENIDLDINDHHLVIDFCKT
CEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCEEEEECCCEEEEECCCEEEEEEECC
HSIDLVIVGPEAPLVAGITDSLNNANICVFGPTQKAAQLEGSKAFTKDLCRKNNIPTGTY
CCEEEEEECCCCCEEEECCCCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHCCCCCCCHH
QCFNDAEKAKAYIRQQGVPIVIKADGLAAGKGVVVAMTMEEAFNAVDACFKSAFGDAGNK
HHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCE
IVVESFLEGEEASFFCLCDGKIAIPFGSAQDHKRVGDGDTGANTGGMGAYSPAPIMTQEM
EEEEHHCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VNRTLNEIVEPVLHSLNQMGSPFKGILFVGLMITKKGPELIEFNVRFGDPECQVLMMRLQ
HHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHH
NDILPILLAAARGNLENTSLQWSKKTALTVVMAAKGYPDSPQKGTVIRNVDKVNALPDVK
CCHHHHHHHHHCCCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCEEECHHHHCCCCCCH
VFQAATVLHNGELIANGGRVLNITATGETILQAQKRAYEAVDFIDWPEGFVRRDIGWRAV
HHHHHHEEECCEEEECCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEE
ARENSIL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]