Click here to switch to the map view.

The map label for this gene is folD

Identifier: 49475193

GI number: 49475193

Start: 449886

End: 450785

Strand: Reverse

Name: folD

Synonym: BH03940

Alternate gene names: 49475193

Gene position: 450785-449886 (Counterclockwise)

Preceding gene: 49475198

Following gene: 49475186

Centisome position: 23.34

GC content: 41.0

Gene sequence:

>900_bases
ATGAACAATATTATTGACGGAAAAAAGCTCGCTGAAAAAATTATTATGAAAGTTAAGGCTGAAACAACTAAATTGCGCAA
CAACTATAAGATACAACCTGGTATCGCTGTCATCATTGTTGGAAACGACCCAGCAAGTCAGGTATATGTCACGTCAAAAA
GCAAAAAAGCCGAAGAATGTGGTTTTTTTTCAATCAAGCATATGATTTCAAAAGAAACACAGGAAAAAGAACTCCTTCAC
CTCATCGCAACATTAAATTCCGATCCCCAAATCCACGGTATTTTGGTACAACTTCCCCTCCCTGATCATATTAATACAAA
CCGCATAACACAAGCCATCGCTTTCCAAAAAGATGTTGATGGTTTTCATTATATCAATATAGGAAAACTCGCAGCAAATG
CACTTGAAGATGCTATCATTCCCTGCACACCAGCTGGTGCCATGATGATGATTGAACAACAATGTGGACAAGACTTATCT
GGTCTTGATGCTGTTGTTGTTGGACGCTCAAATATTGTTGGCAAACCTATGGCCGCCCTCTTAACAGCAGCAAATGCCAC
TGTAACAATCGCTCATAGTCGTACTCGTGATCTTGATGACGTATGTCGTAGCGCTGATATCTTAGTAACAGCTATAGGTC
GCCCACAAATGATTAAAAAAGACTGGGTTAAAAATGGGGCCATTATTATTGACGTGGGCATTAACCGCATTGCAGCACCA
GAGAAAGGTGTGGGAAAGACGCGTCTTGTTGGTGATGTCGATTTTGAAGAAATAAAAGAAAAAGCTGCCGCAATCACCCC
TGTTCCAGGAGGAGTCGGACCAATGACAATCGCTATGCTGATGGTTAATACGCTCAAAGCTGCGGCTCGATTACATAATC
TGCCCATACCAAAATTCTAA

Upstream 100 bases:

>100_bases
TCCTTAGCATTCCCAGATTAATACTGCTACAAAGCTCTATTGTATCGTCAGCAAATGCTTTACAAACAAAACAAATAGGC
CTCATGAAAGGATCGTTCCT

Downstream 100 bases:

>100_bases
GGATTTTATGCACTTTCATTGCATATGCATTACTTTATCTACTTTTCGCTCTTTACTCATGCTATAAGAACAGATGCTCC
TTGATCTGCACGATTAACGG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNNIIDGKKLAEKIIMKVKAETTKLRNNYKIQPGIAVIIVGNDPASQVYVTSKSKKAEECGFFSIKHMISKETQEKELLH
LIATLNSDPQIHGILVQLPLPDHINTNRITQAIAFQKDVDGFHYINIGKLAANALEDAIIPCTPAGAMMMIEQQCGQDLS
GLDAVVVGRSNIVGKPMAALLTAANATVTIAHSRTRDLDDVCRSADILVTAIGRPQMIKKDWVKNGAIIIDVGINRIAAP
EKGVGKTRLVGDVDFEEIKEKAAAITPVPGGVGPMTIAMLMVNTLKAAARLHNLPIPKF

Sequences:

>Translated_299_residues
MNNIIDGKKLAEKIIMKVKAETTKLRNNYKIQPGIAVIIVGNDPASQVYVTSKSKKAEECGFFSIKHMISKETQEKELLH
LIATLNSDPQIHGILVQLPLPDHINTNRITQAIAFQKDVDGFHYINIGKLAANALEDAIIPCTPAGAMMMIEQQCGQDLS
GLDAVVVGRSNIVGKPMAALLTAANATVTIAHSRTRDLDDVCRSADILVTAIGRPQMIKKDWVKNGAIIIDVGINRIAAP
EKGVGKTRLVGDVDFEEIKEKAAAITPVPGGVGPMTIAMLMVNTLKAAARLHNLPIPKF
>Mature_299_residues
MNNIIDGKKLAEKIIMKVKAETTKLRNNYKIQPGIAVIIVGNDPASQVYVTSKSKKAEECGFFSIKHMISKETQEKELLH
LIATLNSDPQIHGILVQLPLPDHINTNRITQAIAFQKDVDGFHYINIGKLAANALEDAIIPCTPAGAMMMIEQQCGQDLS
GLDAVVVGRSNIVGKPMAALLTAANATVTIAHSRTRDLDDVCRSADILVTAIGRPQMIKKDWVKNGAIIIDVGINRIAAP
EKGVGKTRLVGDVDFEEIKEKAAAITPVPGGVGPMTIAMLMVNTLKAAARLHNLPIPKF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=46.3917525773196, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI222418558, Length=298, Percent_Identity=44.6308724832215, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI94721354, Length=301, Percent_Identity=43.8538205980066, Blast_Score=211, Evalue=5e-55,
Organism=Homo sapiens, GI36796743, Length=224, Percent_Identity=26.3392857142857, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1786741, Length=291, Percent_Identity=47.4226804123711, Blast_Score=251, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=41.7808219178082, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=47.4048442906574, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=42.9054054054054, Blast_Score=231, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6322933, Length=296, Percent_Identity=25, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17136816, Length=300, Percent_Identity=48.3333333333333, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI17136818, Length=300, Percent_Identity=48.3333333333333, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=45.7044673539519, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=45.7044673539519, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=45.7044673539519, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=45.7044673539519, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_BARHE (Q6G4F1)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033234.1
- HSSP:   P11586
- ProteinModelPortal:   Q6G4F1
- SMR:   Q6G4F1
- GeneID:   2866038
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH03940
- NMPDR:   fig|283166.1.peg.362
- HOGENOM:   HBG328751
- OMA:   GAMMMIE
- ProtClustDB:   PRK14188
- BioCyc:   BHEN283166:BH03940-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32250; Mature: 32250

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIIDGKKLAEKIIMKVKAETTKLRNNYKIQPGIAVIIVGNDPASQVYVTSKSKKAEEC
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCCEEEEECCCCCHHHC
GFFSIKHMISKETQEKELLHLIATLNSDPQIHGILVQLPLPDHINTNRITQAIAFQKDVD
CHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCC
GFHYINIGKLAANALEDAIIPCTPAGAMMMIEQQCGQDLSGLDAVVVGRSNIVGKPMAAL
CEEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHH
LTAANATVTIAHSRTRDLDDVCRSADILVTAIGRPQMIKKDWVKNGAIIIDVGINRIAAP
HHHCCCEEEEEECCCCCHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCC
EKGVGKTRLVGDVDFEEIKEKAAAITPVPGGVGPMTIAMLMVNTLKAAARLHNLPIPKF
CCCCCCEEEECCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNNIIDGKKLAEKIIMKVKAETTKLRNNYKIQPGIAVIIVGNDPASQVYVTSKSKKAEEC
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCCEEEEECCCCCHHHC
GFFSIKHMISKETQEKELLHLIATLNSDPQIHGILVQLPLPDHINTNRITQAIAFQKDVD
CHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCC
GFHYINIGKLAANALEDAIIPCTPAGAMMMIEQQCGQDLSGLDAVVVGRSNIVGKPMAAL
CEEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHH
LTAANATVTIAHSRTRDLDDVCRSADILVTAIGRPQMIKKDWVKNGAIIIDVGINRIAAP
HHHCCCEEEEEECCCCCHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCC
EKGVGKTRLVGDVDFEEIKEKAAAITPVPGGVGPMTIAMLMVNTLKAAARLHNLPIPKF
CCCCCCEEEECCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA