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The map label for this gene is mscL

Identifier: 49475186

GI number: 49475186

Start: 440413

End: 440826

Strand: Reverse

Name: mscL

Synonym: BH03860

Alternate gene names: 49475186

Gene position: 440826-440413 (Counterclockwise)

Preceding gene: 49475193

Following gene: 49475183

Centisome position: 22.83

GC content: 36.71

Gene sequence:

>414_bases
ATGCTTAAAGAATTCAAAGAATTTGCCCTAAAAGGTAATATGATTGACCTCGCCATCGGTGTAATTATAGGGGGAGCTTT
CGGAGGCTTAGTCAATTCGATTGTCAATGATATTTTTATGCCAATTATTGGTCTCATCACAGGTGGGATTGACTTTTCTA
ACATGTTTATTCAACTTGCCGGTGAAAAACAAGCTACTTTGAGTGCGGCTAAAGCAGCCGGAGCGACTATTAGCTACGGC
AACTTCATTACTTTACTTATCAATTTTCTCATTATTGCTTGGGTTCTCTTCCTCTTTGTTAAAAGTATGAATAAAATACG
CAGAAAACAAGAAGAGGAGGAAACTTCAAAAAAGATGTCTCTAGAACAACAACTTCTCTCAGAAATCAGAGATTTGTTGG
CCAAGAAAAAATAA

Upstream 100 bases:

>100_bases
ATATAATGCTGCAAGAAAGTGAAAAAACTACCCTTTCTTTTTTTATCAAAAGCGATAGTTAAAATTACTGTATGTCTAAT
ATACAAAGGGGAAAAACCTA

Downstream 100 bases:

>100_bases
ATTGATGCGTGAACTTCACGCATAAAATATAGGAAAAAACATTTCTACAGTTGTCCCGTGATTTGGTTTTGAAAATAACA
AAAATTGGCCACCACTTTCT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MLKEFKEFALKGNMIDLAIGVIIGGAFGGLVNSIVNDIFMPIIGLITGGIDFSNMFIQLAGEKQATLSAAKAAGATISYG
NFITLLINFLIIAWVLFLFVKSMNKIRRKQEEEETSKKMSLEQQLLSEIRDLLAKKK

Sequences:

>Translated_137_residues
MLKEFKEFALKGNMIDLAIGVIIGGAFGGLVNSIVNDIFMPIIGLITGGIDFSNMFIQLAGEKQATLSAAKAAGATISYG
NFITLLINFLIIAWVLFLFVKSMNKIRRKQEEEETSKKMSLEQQLLSEIRDLLAKKK
>Mature_137_residues
MLKEFKEFALKGNMIDLAIGVIIGGAFGGLVNSIVNDIFMPIIGLITGGIDFSNMFIQLAGEKQATLSAAKAAGATISYG
NFITLLINFLIIAWVLFLFVKSMNKIRRKQEEEETSKKMSLEQQLLSEIRDLLAKKK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=136, Percent_Identity=44.8529411764706, Blast_Score=121, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_BARHE (Q6G4F8)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033227.1
- ProteinModelPortal:   Q6G4F8
- SMR:   Q6G4F8
- GeneID:   2865360
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH03860
- NMPDR:   fig|283166.1.peg.355
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- PhylomeDB:   Q6G4F8
- ProtClustDB:   PRK13952
- BioCyc:   BHEN283166:BH03860-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15116; Mature: 15116

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x23ebe17c)-; HASH(0x2345f998)-; HASH(0x233f9314)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFKEFALKGNMIDLAIGVIIGGAFGGLVNSIVNDIFMPIIGLITGGIDFSNMFIQLA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
GEKQATLSAAKAAGATISYGNFITLLINFLIIAWVLFLFVKSMNKIRRKQEEEETSKKMS
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEQQLLSEIRDLLAKKK
HHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MLKEFKEFALKGNMIDLAIGVIIGGAFGGLVNSIVNDIFMPIIGLITGGIDFSNMFIQLA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
GEKQATLSAAKAAGATISYGNFITLLINFLIIAWVLFLFVKSMNKIRRKQEEEETSKKMS
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEQQLLSEIRDLLAKKK
HHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA