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The map label for this gene is trmB

Identifier: 49475031

GI number: 49475031

Start: 295269

End: 295970

Strand: Reverse

Name: trmB

Synonym: BH02190

Alternate gene names: 49475031

Gene position: 295970-295269 (Counterclockwise)

Preceding gene: 49475032

Following gene: 49475030

Centisome position: 15.33

GC content: 37.89

Gene sequence:

>702_bases
ATGATTGATCATAATATACGTACAGGTAAGGCCTTTTTTGGTCGTCGAAAAGGAAAACGGCTACGGAACAGTCAGCTTGT
ACTTATAAAAAAGCTTTTTCCAACTCTGGACATTAACTTAAATAACTCTGTACCCCTAAACTTAACGTCCTTATTTTCCA
GAAAAGTAAAAGAAGTTCGTCTTGAAATTGGCTTTGGTGGAGGCGAACATTTGCTCCATGAAATGAAACATTTTCCACAA
ACCGGTTTTATCGGAATCGAGCCCTTTATCAATGGCATGGCAAAAATGTTAATGTCTCTTGAAGAACATAAACAGTATCA
AAATCAACTTCGCCTTTATAATGATGATGCCACAGGCCTCCTTGATTGGCTCCCAAATGCATCACTTGATGGAATAGACC
TCTTCTATCCTGATCCATGGCCTAAAAAAAAGCATTGGAAAAGACGCTTTATCAACATGAAAAATCTTAATCGTTTTGCT
CGCGTTCTTAAAACAGGAAAAAAATTCCGCTTTGCCAGCGATATAGAAAGTTATGTAAACTGGACCCTTTACCATTGTTC
AAACCATCACAGTTTTGAATGGGAAGCAAAAAATCCTAAGGATTGGAAAACACCTTATACGCTATGGTCAGGCACTCGTT
ATGAAGCAAAAGCTTTACGCGAAGGACGAGCACCTACTTATCTTACTTTTATCAAGAAATAG

Upstream 100 bases:

>100_bases
CCGCTTATGGTCATTTTGGACGCAAACCAAAACGTGATGGTTCATTTTCATGGGAAAAAACTGATCTCATTAAAACGCTT
CAAAAAATGATTAAGGTACC

Downstream 100 bases:

>100_bases
ATGATGAAACGCTCTTCTTATAAAAAAACTTGAAAAATTTTTAATTTAAGAGTATCAATGGTTAAATTCTTGGTCTTATG
ATGAAGAGTGGGCCGTCTGG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MIDHNIRTGKAFFGRRKGKRLRNSQLVLIKKLFPTLDINLNNSVPLNLTSLFSRKVKEVRLEIGFGGGEHLLHEMKHFPQ
TGFIGIEPFINGMAKMLMSLEEHKQYQNQLRLYNDDATGLLDWLPNASLDGIDLFYPDPWPKKKHWKRRFINMKNLNRFA
RVLKTGKKFRFASDIESYVNWTLYHCSNHHSFEWEAKNPKDWKTPYTLWSGTRYEAKALREGRAPTYLTFIKK

Sequences:

>Translated_233_residues
MIDHNIRTGKAFFGRRKGKRLRNSQLVLIKKLFPTLDINLNNSVPLNLTSLFSRKVKEVRLEIGFGGGEHLLHEMKHFPQ
TGFIGIEPFINGMAKMLMSLEEHKQYQNQLRLYNDDATGLLDWLPNASLDGIDLFYPDPWPKKKHWKRRFINMKNLNRFA
RVLKTGKKFRFASDIESYVNWTLYHCSNHHSFEWEAKNPKDWKTPYTLWSGTRYEAKALREGRAPTYLTFIKK
>Mature_233_residues
MIDHNIRTGKAFFGRRKGKRLRNSQLVLIKKLFPTLDINLNNSVPLNLTSLFSRKVKEVRLEIGFGGGEHLLHEMKHFPQ
TGFIGIEPFINGMAKMLMSLEEHKQYQNQLRLYNDDATGLLDWLPNASLDGIDLFYPDPWPKKKHWKRRFINMKNLNRFA
RVLKTGKKFRFASDIESYVNWTLYHCSNHHSFEWEAKNPKDWKTPYTLWSGTRYEAKALREGRAPTYLTFIKK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=172, Percent_Identity=33.7209302325581, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_BARHE (Q6G4W3)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033072.1
- ProteinModelPortal:   Q6G4W3
- SMR:   Q6G4W3
- GeneID:   2864622
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH02190
- NMPDR:   fig|283166.1.peg.200
- HOGENOM:   HBG322782
- OMA:   YEAKALR
- PhylomeDB:   Q6G4W3
- ProtClustDB:   PRK00121
- BioCyc:   BHEN283166:BH02190-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27515; Mature: 27515

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: NA

Important sites: BINDING 62-62 BINDING 87-87 BINDING 116-116 BINDING 138-138 BINDING 142-142 BINDING 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDHNIRTGKAFFGRRKGKRLRNSQLVLIKKLFPTLDINLNNSVPLNLTSLFSRKVKEVR
CCCCCCCCCHHHHHHHHCCHHCCCCEEEEEHHCCEEEECCCCCCCCHHHHHHHHHHHHHE
LEIGFGGGEHLLHEMKHFPQTGFIGIEPFINGMAKMLMSLEEHKQYQNQLRLYNDDATGL
EEECCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHH
LDWLPNASLDGIDLFYPDPWPKKKHWKRRFINMKNLNRFARVLKTGKKFRFASDIESYVN
HHHCCCCCCCCEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHC
WTLYHCSNHHSFEWEAKNPKDWKTPYTLWSGTRYEAKALREGRAPTYLTFIKK
EEEEEECCCCCEEECCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MIDHNIRTGKAFFGRRKGKRLRNSQLVLIKKLFPTLDINLNNSVPLNLTSLFSRKVKEVR
CCCCCCCCCHHHHHHHHCCHHCCCCEEEEEHHCCEEEECCCCCCCCHHHHHHHHHHHHHE
LEIGFGGGEHLLHEMKHFPQTGFIGIEPFINGMAKMLMSLEEHKQYQNQLRLYNDDATGL
EEECCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHH
LDWLPNASLDGIDLFYPDPWPKKKHWKRRFINMKNLNRFARVLKTGKKFRFASDIESYVN
HHHCCCCCCCCEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHC
WTLYHCSNHHSFEWEAKNPKDWKTPYTLWSGTRYEAKALREGRAPTYLTFIKK
EEEEEECCCCCEEECCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA