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The map label for this gene is metK [H]

Identifier: 49475032

GI number: 49475032

Start: 295967

End: 297235

Strand: Reverse

Name: metK [H]

Synonym: BH02200

Alternate gene names: 49475032

Gene position: 297235-295967 (Counterclockwise)

Preceding gene: 49475033

Following gene: 49475031

Centisome position: 15.39

GC content: 40.82

Gene sequence:

>1269_bases
ATGTCGCATCAAGATTATCTTTTTACAAGTGAATCAGTATCGGAGGGACATCCTGACAAAATTTGCGATCGTATTTCTGA
TGAGATCGTTGATATGATCTATAAAGAAGCTCAAAAAACTGGTACTGATCCATGGTTAGTACGCATCGCTTGCGAAACCT
TAGTAAGTATCAACCGCGTTGTTATTGCTGGTGAGGTACGTGTTCCTGATACACTCTTAAAAAGAGATAAAAATAGGGAA
TTTATCTACGATGAAACTGGTTTACCACGCATTAATCCTGCACGTTTTCGTGCAGTAGCACGTCGTGCTATTCGTGCAAT
TGGTTATGAACAAGTAGGCTTTCATTGGAAAACCGTTAAAATTGATGTTCTTTTGCGCCCACAATCAGTTGACATTGCAC
GGGGGGTTGATAATGCTAGGGATCGGCATGATGAAGAAGGAGCAGGTGATCAGGGCATCATGTTCGGATATGCTTGTCGT
GAAACACCAGATCTCATGCCCGCGCCAATTTATTATGCACATAAAATTCTCAAACTTCTTGCTGAAGCGCGTCATAAAGG
AGAAGGAGAAACTGGAAAATTAGGGCCAGATGCAAAAAGTCAAATAACAATACGGTACAAGAATGGAAAGCCTATAGAAG
TAACATCCATCGTTCTTTCTACACAGCATTTAGATGAAAGTTGGGATTCCCATAAAGTCCGCACAGTGGTTGAGCCTTAT
ATCCATCAAGCTTTGCATGATATTCCTATTTCCGATCAATGCAGTTGGTATATTAACCCAACAGGAAAATTTGTTATCGG
TGGCCCCCAATGTGATGCTGGACTGACAGGACGTAAAATTATCGTTGACACTTATGGAGGTGCAGCCCCCCATGGTGGAG
GAGCTTTTTCAGGCAAAGATACAACAAAAGTTGATCGTTCTGCAGCTTATGCTGCACGCTATCTGGCTAAGAATATTGTT
GCAGCTGACTTAGCAGAACGATGCACTATTCAACTCTCCTATGCAATCGGCGTTGCACAACCCTTATCCATTTATATTAA
TTTCCATGGAACAGGAAAAGTCGATGAAAGTATTATCGAAGCAGTAATCCGGAAAATAATGGATCTTTCACCGACAGGAA
TCCGGAAACATTTAGATCTCAACAAACCGATTTATACAAAAACAGCCGCTTATGGTCATTTTGGACGCAAACCAAAACGT
GATGGTTCATTTTCATGGGAAAAAACTGATCTCATTAAAACGCTTCAAAAAATGATTAAGGTACCATGA

Upstream 100 bases:

>100_bases
TCTCGCTCACCTTATCAATCAACATTGATGATTTGTCATGGGATTGGCAAATAAGGGCAATTTTAATATAGAGTTGGTTT
TCTTTGTTTAAGGAGTTCCT

Downstream 100 bases:

>100_bases
TTGATCATAATATACGTACAGGTAAGGCCTTTTTTGGTCGTCGAAAAGGAAAACGGCTACGGAACAGTCAGCTTGTACTT
ATAAAAAAGCTTTTTCCAAC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MSHQDYLFTSESVSEGHPDKICDRISDEIVDMIYKEAQKTGTDPWLVRIACETLVSINRVVIAGEVRVPDTLLKRDKNRE
FIYDETGLPRINPARFRAVARRAIRAIGYEQVGFHWKTVKIDVLLRPQSVDIARGVDNARDRHDEEGAGDQGIMFGYACR
ETPDLMPAPIYYAHKILKLLAEARHKGEGETGKLGPDAKSQITIRYKNGKPIEVTSIVLSTQHLDESWDSHKVRTVVEPY
IHQALHDIPISDQCSWYINPTGKFVIGGPQCDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNIV
AADLAERCTIQLSYAIGVAQPLSIYINFHGTGKVDESIIEAVIRKIMDLSPTGIRKHLDLNKPIYTKTAAYGHFGRKPKR
DGSFSWEKTDLIKTLQKMIKVP

Sequences:

>Translated_422_residues
MSHQDYLFTSESVSEGHPDKICDRISDEIVDMIYKEAQKTGTDPWLVRIACETLVSINRVVIAGEVRVPDTLLKRDKNRE
FIYDETGLPRINPARFRAVARRAIRAIGYEQVGFHWKTVKIDVLLRPQSVDIARGVDNARDRHDEEGAGDQGIMFGYACR
ETPDLMPAPIYYAHKILKLLAEARHKGEGETGKLGPDAKSQITIRYKNGKPIEVTSIVLSTQHLDESWDSHKVRTVVEPY
IHQALHDIPISDQCSWYINPTGKFVIGGPQCDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNIV
AADLAERCTIQLSYAIGVAQPLSIYINFHGTGKVDESIIEAVIRKIMDLSPTGIRKHLDLNKPIYTKTAAYGHFGRKPKR
DGSFSWEKTDLIKTLQKMIKVP
>Mature_421_residues
SHQDYLFTSESVSEGHPDKICDRISDEIVDMIYKEAQKTGTDPWLVRIACETLVSINRVVIAGEVRVPDTLLKRDKNREF
IYDETGLPRINPARFRAVARRAIRAIGYEQVGFHWKTVKIDVLLRPQSVDIARGVDNARDRHDEEGAGDQGIMFGYACRE
TPDLMPAPIYYAHKILKLLAEARHKGEGETGKLGPDAKSQITIRYKNGKPIEVTSIVLSTQHLDESWDSHKVRTVVEPYI
HQALHDIPISDQCSWYINPTGKFVIGGPQCDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNIVA
ADLAERCTIQLSYAIGVAQPLSIYINFHGTGKVDESIIEAVIRKIMDLSPTGIRKHLDLNKPIYTKTAAYGHFGRKPKRD
GSFSWEKTDLIKTLQKMIKVP

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=414, Percent_Identity=45.8937198067633, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI4557737, Length=414, Percent_Identity=44.6859903381643, Blast_Score=288, Evalue=6e-78,
Organism=Escherichia coli, GI1789311, Length=413, Percent_Identity=47.9418886198547, Blast_Score=347, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI17538498, Length=426, Percent_Identity=42.9577464788732, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17538494, Length=415, Percent_Identity=43.855421686747, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25145633, Length=419, Percent_Identity=42.4821002386635, Blast_Score=284, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI17551082, Length=414, Percent_Identity=42.0289855072464, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI32565911, Length=331, Percent_Identity=46.2235649546828, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI25145635, Length=324, Percent_Identity=45.3703703703704, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI32565905, Length=301, Percent_Identity=48.5049833887043, Blast_Score=237, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=48.5049833887043, Blast_Score=236, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6320710, Length=413, Percent_Identity=44.5520581113801, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6323209, Length=415, Percent_Identity=43.6144578313253, Blast_Score=294, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24580527, Length=412, Percent_Identity=43.9320388349515, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24580525, Length=412, Percent_Identity=43.9320388349515, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI45552159, Length=412, Percent_Identity=43.6893203883495, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24580529, Length=412, Percent_Identity=43.6893203883495, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24580537, Length=412, Percent_Identity=43.6893203883495, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24580533, Length=412, Percent_Identity=43.6893203883495, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24580535, Length=412, Percent_Identity=43.6893203883495, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24580531, Length=412, Percent_Identity=43.6893203883495, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24580541, Length=413, Percent_Identity=41.8886198547215, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24580539, Length=148, Percent_Identity=36.4864864864865, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 47024; Mature: 46893

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQDYLFTSESVSEGHPDKICDRISDEIVDMIYKEAQKTGTDPWLVRIACETLVSINRV
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEE
VIAGEVRVPDTLLKRDKNREFIYDETGLPRINPARFRAVARRAIRAIGYEQVGFHWKTVK
EEEECCCCCHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCEEEEEE
IDVLLRPQSVDIARGVDNARDRHDEEGAGDQGIMFGYACRETPDLMPAPIYYAHKILKLL
EEEEECCCCCHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
AEARHKGEGETGKLGPDAKSQITIRYKNGKPIEVTSIVLSTQHLDESWDSHKVRTVVEPY
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEHHHHHCCCCCCHHHHHHHHH
IHQALHDIPISDQCSWYINPTGKFVIGGPQCDAGLTGRKIIVDTYGGAAPHGGGAFSGKD
HHHHHHCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
TTKVDRSAAYAARYLAKNIVAADLAERCTIQLSYAIGVAQPLSIYINFHGTGKVDESIIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEEEEEEECCCCCHHHHHHH
AVIRKIMDLSPTGIRKHLDLNKPIYTKTAAYGHFGRKPKRDGSFSWEKTDLIKTLQKMIK
HHHHHHHCCCCCCHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
VP
CC
>Mature Secondary Structure 
SHQDYLFTSESVSEGHPDKICDRISDEIVDMIYKEAQKTGTDPWLVRIACETLVSINRV
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEE
VIAGEVRVPDTLLKRDKNREFIYDETGLPRINPARFRAVARRAIRAIGYEQVGFHWKTVK
EEEECCCCCHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCEEEEEE
IDVLLRPQSVDIARGVDNARDRHDEEGAGDQGIMFGYACRETPDLMPAPIYYAHKILKLL
EEEEECCCCCHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
AEARHKGEGETGKLGPDAKSQITIRYKNGKPIEVTSIVLSTQHLDESWDSHKVRTVVEPY
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEHHHHHCCCCCCHHHHHHHHH
IHQALHDIPISDQCSWYINPTGKFVIGGPQCDAGLTGRKIIVDTYGGAAPHGGGAFSGKD
HHHHHHCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
TTKVDRSAAYAARYLAKNIVAADLAERCTIQLSYAIGVAQPLSIYINFHGTGKVDESIIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEEEEEEECCCCCHHHHHHH
AVIRKIMDLSPTGIRKHLDLNKPIYTKTAAYGHFGRKPKRDGSFSWEKTDLIKTLQKMIK
HHHHHHHCCCCCCHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
VP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA