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The map label for this gene is prfA

Identifier: 49475009

GI number: 49475009

Start: 266427

End: 267506

Strand: Reverse

Name: prfA

Synonym: BH01970

Alternate gene names: 49475009

Gene position: 267506-266427 (Counterclockwise)

Preceding gene: 49475010

Following gene: 49475008

Centisome position: 13.85

GC content: 41.48

Gene sequence:

>1080_bases
ATGGTTTCTTTGCCACAAGATCGTATGAGACAGCTTGAAAAGCGCTTTGAAATAATTGAAAGCCAAATGGCTAAAAACCC
GAATGCTGAAATTTATGTAAAATTAGCTTCTGAATATGCTGAATTGCAACCAATTGTTAGTTCCATACGAGCATTAAATG
CTCTTTACGGAGAGATCACAGATCTAGAAACTATCATCAGTGATAAACTCACAGATGTAGAGATGCGTACTCTTGCTCAA
GAAGAGCTCCCATTATTATGTCAGAAAATGGAACAACTTGAGCAAAAGCTTCAGATTCTTCTTTTGCCCAAAGACGTTGC
TGATGAAAAAAGCGCCATTGTTGAAATTCGAGCCGGGACAGGTGGATCAGAAGCAGCACTTTTCGCCGGTGATCTCTTTC
GCATGTATGAACGTTATGCCGCTTCTCGTAACTGGAAAGTGGAAGTTGTTTCGCTTAATGAAGGGGAAGTTGGTGGATAT
AAAGAAATTATTGCTACTATTTCAGGGAGAGGTGTCTTTTCTAAACTCAAATTTGAATCAGGTGTTCATCGTGTGCAACG
CATCCCTGAAACAGAAACAGGTGGACGCATCCATACATCTGCTGCTACCGTTGCAGTGCTTCCAGAAGCTGAAGAAATTG
ATATTGAAATTCGTCCAGAAGATATTCGCATTGATACAATGCGTGCCTCTGGAGCAGGAGGACAGCATGTCAATACAACG
GATTCCGCTGTTCGTATCACACATATTCCAACTGGGATTATGGTCGTACAAGCAGAAAAATCACAGCATCAAAATCGCGC
ACGGGCACTGCAAATTTTACGTGCACGCTTATTTGATATTGAACGACAAAAAGCGGAAAATGAACGCTCAGCATCCCGTA
AAAGCCAAGTTGGATCTGGTGATCGCTCAGAACGTATTCGTACCTATAATTTTCCACAAGGACGTGTCACGGATCATCGT
ATTAATCTCACTCTTTATAAACTTGACCGCGTTTTAGAAGGAGATCTTGATGAACTTATTCATGCACTCATATCCGATCA
CCAAACAGCCCTCCTTATGGAAATGGATCATCATGAATGA

Upstream 100 bases:

>100_bases
AAGATTTTCTACTTTATTACCCCTTTTTATAGGATGCTATAAAACACTCTATCACTAATATTACAACAAAAGTATTATAA
TAAATGAGGCCAATATTATC

Downstream 100 bases:

>100_bases
ACAAACATTCTCTCAACAATGTCATTCGACAAACTCAAGAAAAATTGCGCTCTCAAGGAGTTTCTGAAGCCAATCTTGAT
GCAAAAATACTGGTCGAATG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MVSLPQDRMRQLEKRFEIIESQMAKNPNAEIYVKLASEYAELQPIVSSIRALNALYGEITDLETIISDKLTDVEMRTLAQ
EELPLLCQKMEQLEQKLQILLLPKDVADEKSAIVEIRAGTGGSEAALFAGDLFRMYERYAASRNWKVEVVSLNEGEVGGY
KEIIATISGRGVFSKLKFESGVHRVQRIPETETGGRIHTSAATVAVLPEAEEIDIEIRPEDIRIDTMRASGAGGQHVNTT
DSAVRITHIPTGIMVVQAEKSQHQNRARALQILRARLFDIERQKAENERSASRKSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVLEGDLDELIHALISDHQTALLMEMDHHE

Sequences:

>Translated_359_residues
MVSLPQDRMRQLEKRFEIIESQMAKNPNAEIYVKLASEYAELQPIVSSIRALNALYGEITDLETIISDKLTDVEMRTLAQ
EELPLLCQKMEQLEQKLQILLLPKDVADEKSAIVEIRAGTGGSEAALFAGDLFRMYERYAASRNWKVEVVSLNEGEVGGY
KEIIATISGRGVFSKLKFESGVHRVQRIPETETGGRIHTSAATVAVLPEAEEIDIEIRPEDIRIDTMRASGAGGQHVNTT
DSAVRITHIPTGIMVVQAEKSQHQNRARALQILRARLFDIERQKAENERSASRKSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVLEGDLDELIHALISDHQTALLMEMDHHE
>Mature_359_residues
MVSLPQDRMRQLEKRFEIIESQMAKNPNAEIYVKLASEYAELQPIVSSIRALNALYGEITDLETIISDKLTDVEMRTLAQ
EELPLLCQKMEQLEQKLQILLLPKDVADEKSAIVEIRAGTGGSEAALFAGDLFRMYERYAASRNWKVEVVSLNEGEVGGY
KEIIATISGRGVFSKLKFESGVHRVQRIPETETGGRIHTSAATVAVLPEAEEIDIEIRPEDIRIDTMRASGAGGQHVNTT
DSAVRITHIPTGIMVVQAEKSQHQNRARALQILRARLFDIERQKAENERSASRKSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVLEGDLDELIHALISDHQTALLMEMDHHE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=47.682119205298, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI34577120, Length=350, Percent_Identity=40.2857142857143, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=46.8599033816425, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1787462, Length=326, Percent_Identity=58.2822085889571, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI2367172, Length=312, Percent_Identity=39.7435897435897, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=275, Percent_Identity=41.8181818181818, Blast_Score=195, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=46.8354430379747, Blast_Score=256, Evalue=5e-69,
Organism=Drosophila melanogaster, GI19921226, Length=333, Percent_Identity=41.1411411411411, Blast_Score=231, Evalue=7e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_BARHE (Q6G5T7)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033050.1
- ProteinModelPortal:   Q6G5T7
- SMR:   Q6G5T7
- GeneID:   2865525
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH01970
- NMPDR:   fig|283166.1.peg.178
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q6G5T7
- ProtClustDB:   PRK00591
- BioCyc:   BHEN283166:BH01970-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40452; Mature: 40452

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLPQDRMRQLEKRFEIIESQMAKNPNAEIYVKLASEYAELQPIVSSIRALNALYGEIT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
DLETIISDKLTDVEMRTLAQEELPLLCQKMEQLEQKLQILLLPKDVADEKSAIVEIRAGT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECCC
GGSEAALFAGDLFRMYERYAASRNWKVEVVSLNEGEVGGYKEIIATISGRGVFSKLKFES
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHH
GVHRVQRIPETETGGRIHTSAATVAVLPEAEEIDIEIRPEDIRIDTMRASGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEECCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCC
DSAVRITHIPTGIMVVQAEKSQHQNRARALQILRARLFDIERQKAENERSASRKSQVGSG
CCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQGRVTDHRINLTLYKLDRVLEGDLDELIHALISDHQTALLMEMDHHE
CCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCC
>Mature Secondary Structure
MVSLPQDRMRQLEKRFEIIESQMAKNPNAEIYVKLASEYAELQPIVSSIRALNALYGEIT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
DLETIISDKLTDVEMRTLAQEELPLLCQKMEQLEQKLQILLLPKDVADEKSAIVEIRAGT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECCC
GGSEAALFAGDLFRMYERYAASRNWKVEVVSLNEGEVGGYKEIIATISGRGVFSKLKFES
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHH
GVHRVQRIPETETGGRIHTSAATVAVLPEAEEIDIEIRPEDIRIDTMRASGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEECCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCC
DSAVRITHIPTGIMVVQAEKSQHQNRARALQILRARLFDIERQKAENERSASRKSQVGSG
CCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQGRVTDHRINLTLYKLDRVLEGDLDELIHALISDHQTALLMEMDHHE
CCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA