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The map label for this gene is gyrB [H]

Identifier: 49474867

GI number: 49474867

Start: 53453

End: 55885

Strand: Reverse

Name: gyrB [H]

Synonym: BH00410

Alternate gene names: 49474867

Gene position: 55885-53453 (Counterclockwise)

Preceding gene: 49474869

Following gene: 49474866

Centisome position: 2.89

GC content: 42.62

Gene sequence:

>2433_bases
ATGAGTGATGAAATAACCTCACAAATGGAGGGTGACGACTATGGTGCCGCTTCTATCAAAGTTCTCAAAGGTCTCGATGC
TGTACGTAAACGTCCTGGTATGTATATCGGCGATACAGATGATGGCTCAGGCTTGCATCATATGGTCTATGAGGTTGTAG
ACAACGCTATTGATGAAGCCTTAGCAGGTCATGCTACTCTTGTAAACGTGACACTTCATGCTGATGGCTCTTGTTCTGTT
CGTGATAATGGACGCGGAATCCCTACCGATATTCATCCAACAGAAGGTGTTTCAGCAGCTGAGGTTATCATGACCCAACT
TCATGCCGGCGGAAAGTTTGATCAAAATTCTTACAAAGTTTCGGGAGGATTACATGGCGTTGGCGTTTCTGTTGTGAATG
CACTCTCAGTTTGGTTGCAACTGCGAATCAGGCGCAATGGCAAAATCCATGAAATGTCATTTACTCATGGAGTTGCCGAT
GCTCCACTAAAAATTGTCGGTGATTGCGGCAAAGAAAGCGGATCAGAGATCAGGTTTTTACCAAGTTCTGAAACCTTTAC
CATGGTTGAATTTGATTTCGAAACCTTGGAACGTCGTTTACGGGAATTGGCTTTTTTGAATTCTGGTGTGCATATCCTTC
TTGTCGATCAACGCCATGCCGACATCAAATCCGTTGAATTGCATTATGAGGGTGGATTAACTGAATTTGTCAAATATATT
GACCAATCGAAAAAAGCGCTCCTCGATCATCCTATTTATATTACAAGTGAAAAAGATGGGATGAACGTTGAGGTTGCTTT
ATGGTGGAATGATTCCTATCATGAAAAAGTCCTGTGTTTTACCAATAATATCCCTCAGCGTGATGGGGGAACCCATTTGG
TGGGTTTCCGCAGTGCTCTTACACGCCAAATTAATGGTTATGCTGAATCTTCAGGCATTGTAAAAAAGGAAAAAGTAAAC
CTCACCGGTGATGACTGCCGCGAAGGACTCACTGCTATTCTTTCCGTTAAAGTTCCCGATCCAAAATTTTCTTCGCAAAC
AAAAGATAAATTGGTTTCCTCTGAAGTGCGGCCTATTGTTGAAAATTTGGTGAATGAAGGTCTCTCTGCATGGCTAGAAG
AACATCCTAATGAAGCAAAAATTGTTACAAGTAAAGTAGTGGAGGCTGCGGCTGCTCGTGAGGCTGCGCGCAAAGCACGT
GAACTCACAAGACGAAAAGGAGCCCTCGATATCACCTCTTTGCCTGGAAAACTCGCCGATTGTCAAGAACGTGATCCAGC
AAAATCGGAGATCTTCATCGTTGAGGGAGATTCTGCTGGTGGTTCAGCAAAAAGTGGGCGCTCACGCCAAAATCAAGCAA
TTTTGCCTCTGCGTGGTAAAATTCTCAATGTCGAACGAGCGCGTTTCGACCGGATGCTCTCATCTGACATGATTGGAACA
CTCATCACTGCTCTTGGAACATCCATTGGTAAAGATGAATTCTCACCAGATAAATTACGCTACCATAAAATCATCATTAT
GACCGATGCTGACGTTGATGGTGCGCATATACGCACTCTGCTCCTTACTTTCTTTTTTAGACAAATGCCTGAATTGATTG
AGCGTGGACACCTTTATATCGCTCAACCGCCTCTTTATAAAGTAACACGGGGGAAGTCTTTTCAATATATCAAAAATGAA
GCAGCGTTCGAAGAATTTTTGATCGATACTGGACTAGAAGAAACAACATTAGAACTCTCAACAGGAGAAGTTCGTGCAGG
TGCTGATTTGCGCCAACTTGCCGAAGATGCTCGCGTGTTACGCCAACTTTTAAATGGTCTTCATACGCGCTATAATCGCA
ATATTGTTGAGCAAGCAGCAATTGTTGGGGCTTTTAATCCTGAGGCTTTTGCAACACCAGAAAAAGCCAAAAAAACAGCT
GATAATGTAGCAAGTCGCCTCGATTTGATTGCCGATGATATGGAACGCGGGTGGAATGGTGAGTTCACACCAAATGGGGA
TTTGTATTGTGAACGCATTTTACGCGGGGTTAAAGATGTCATTACCCTTGATGCAGGACTTATAAATTCAGTTGATGCAC
GCCAAATTGATCGTATTTCTCAAAATCTTATGGAAACATATAGTTCTCCCCCTCTCTTGCATCGTAAAGATAAATCAGAA
CGTATTTTGGGACCTATGAGTCTCTTAGAAAGCATTTTTACAAATGGTAAAAAAGGCATCACGCTTCAACGTTATAAAGG
TCTTGGAGAAATGAATGCTGAACAGCTTTGGGAAACAACGCTTAATCCTGATGCGCGCGCTCTTTTACAAGTTAAAATCA
ATGATGCAACTGATGCAGATTCGCTCTTTTCCCGTTTGATGGGTGATGAAGTTGAACCACGGCGGGCTTTTATTCAAGAT
AATGCCTTAAGTGTTGCTAATCTTGATATCTAA

Upstream 100 bases:

>100_bases
AAACGTTTAATGTGGTTATTCCATAAATAACTGGCAAAAATATTAAGCTCTGAAACAGCTGATGAAATATCGCAAATTAA
ATACAGGAATGGATTAACTT

Downstream 100 bases:

>100_bases
AGGTACTCTTTTTCTAGCTGTATAAACGTCGTGGTTTTATAGAGGGCTTTTGCATATTTGAACATTCCTTCTACACTTCA
TTTACGACAAAAGAAAAAAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MSDEITSQMEGDDYGAASIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATLVNVTLHADGSCSV
RDNGRGIPTDIHPTEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSVWLQLRIRRNGKIHEMSFTHGVAD
APLKIVGDCGKESGSEIRFLPSSETFTMVEFDFETLERRLRELAFLNSGVHILLVDQRHADIKSVELHYEGGLTEFVKYI
DQSKKALLDHPIYITSEKDGMNVEVALWWNDSYHEKVLCFTNNIPQRDGGTHLVGFRSALTRQINGYAESSGIVKKEKVN
LTGDDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPIVENLVNEGLSAWLEEHPNEAKIVTSKVVEAAAAREAARKAR
ELTRRKGALDITSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKSGRSRQNQAILPLRGKILNVERARFDRMLSSDMIGT
LITALGTSIGKDEFSPDKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYIAQPPLYKVTRGKSFQYIKNE
AAFEEFLIDTGLEETTLELSTGEVRAGADLRQLAEDARVLRQLLNGLHTRYNRNIVEQAAIVGAFNPEAFATPEKAKKTA
DNVASRLDLIADDMERGWNGEFTPNGDLYCERILRGVKDVITLDAGLINSVDARQIDRISQNLMETYSSPPLLHRKDKSE
RILGPMSLLESIFTNGKKGITLQRYKGLGEMNAEQLWETTLNPDARALLQVKINDATDADSLFSRLMGDEVEPRRAFIQD
NALSVANLDI

Sequences:

>Translated_810_residues
MSDEITSQMEGDDYGAASIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATLVNVTLHADGSCSV
RDNGRGIPTDIHPTEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSVWLQLRIRRNGKIHEMSFTHGVAD
APLKIVGDCGKESGSEIRFLPSSETFTMVEFDFETLERRLRELAFLNSGVHILLVDQRHADIKSVELHYEGGLTEFVKYI
DQSKKALLDHPIYITSEKDGMNVEVALWWNDSYHEKVLCFTNNIPQRDGGTHLVGFRSALTRQINGYAESSGIVKKEKVN
LTGDDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPIVENLVNEGLSAWLEEHPNEAKIVTSKVVEAAAAREAARKAR
ELTRRKGALDITSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKSGRSRQNQAILPLRGKILNVERARFDRMLSSDMIGT
LITALGTSIGKDEFSPDKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYIAQPPLYKVTRGKSFQYIKNE
AAFEEFLIDTGLEETTLELSTGEVRAGADLRQLAEDARVLRQLLNGLHTRYNRNIVEQAAIVGAFNPEAFATPEKAKKTA
DNVASRLDLIADDMERGWNGEFTPNGDLYCERILRGVKDVITLDAGLINSVDARQIDRISQNLMETYSSPPLLHRKDKSE
RILGPMSLLESIFTNGKKGITLQRYKGLGEMNAEQLWETTLNPDARALLQVKINDATDADSLFSRLMGDEVEPRRAFIQD
NALSVANLDI
>Mature_809_residues
SDEITSQMEGDDYGAASIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATLVNVTLHADGSCSVR
DNGRGIPTDIHPTEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSVWLQLRIRRNGKIHEMSFTHGVADA
PLKIVGDCGKESGSEIRFLPSSETFTMVEFDFETLERRLRELAFLNSGVHILLVDQRHADIKSVELHYEGGLTEFVKYID
QSKKALLDHPIYITSEKDGMNVEVALWWNDSYHEKVLCFTNNIPQRDGGTHLVGFRSALTRQINGYAESSGIVKKEKVNL
TGDDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPIVENLVNEGLSAWLEEHPNEAKIVTSKVVEAAAAREAARKARE
LTRRKGALDITSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKSGRSRQNQAILPLRGKILNVERARFDRMLSSDMIGTL
ITALGTSIGKDEFSPDKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYIAQPPLYKVTRGKSFQYIKNEA
AFEEFLIDTGLEETTLELSTGEVRAGADLRQLAEDARVLRQLLNGLHTRYNRNIVEQAAIVGAFNPEAFATPEKAKKTAD
NVASRLDLIADDMERGWNGEFTPNGDLYCERILRGVKDVITLDAGLINSVDARQIDRISQNLMETYSSPPLLHRKDKSER
ILGPMSLLESIFTNGKKGITLQRYKGLGEMNAEQLWETTLNPDARALLQVKINDATDADSLFSRLMGDEVEPRRAFIQDN
ALSVANLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=566, Percent_Identity=25.2650176678445, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI19913408, Length=554, Percent_Identity=25.2707581227437, Blast_Score=125, Evalue=2e-28,
Organism=Escherichia coli, GI48994957, Length=808, Percent_Identity=56.8069306930693, Blast_Score=888, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=561, Percent_Identity=39.9286987522282, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=539, Percent_Identity=25.0463821892393, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI212645845, Length=524, Percent_Identity=23.4732824427481, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=139, Percent_Identity=33.0935251798561, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=588, Percent_Identity=26.0204081632653, Blast_Score=120, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17136538, Length=557, Percent_Identity=26.032315978456, Blast_Score=137, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89567; Mature: 89435

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEITSQMEGDDYGAASIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEA
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
LAGHATLVNVTLHADGSCSVRDNGRGIPTDIHPTEGVSAAEVIMTQLHAGGKFDQNSYKV
HCCCEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGVSVVNALSVWLQLRIRRNGKIHEMSFTHGVADAPLKIVGDCGKESGSEIRFL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHEECCCCCCCCEEEEE
PSSETFTMVEFDFETLERRLRELAFLNSGVHILLVDQRHADIKSVELHYEGGLTEFVKYI
CCCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHH
DQSKKALLDHPIYITSEKDGMNVEVALWWNDSYHEKVLCFTNNIPQRDGGTHLVGFRSAL
HHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCEEEHHHHHH
TRQINGYAESSGIVKKEKVNLTGDDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPIV
HHHHCCHHHCCCCEEEEEECCCHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ENLVNEGLSAWLEEHPNEAKIVTSKVVEAAAAREAARKARELTRRKGALDITSLPGKLAD
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
CQERDPAKSEIFIVEGDSAGGSAKSGRSRQNQAILPLRGKILNVERARFDRMLSSDMIGT
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCEECHHHHHHHHHHHHHHHHH
LITALGTSIGKDEFSPDKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYI
HHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
AQPPLYKVTRGKSFQYIKNEAAFEEFLIDTGLEETTLELSTGEVRAGADLRQLAEDARVL
ECCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
RQLLNGLHTRYNRNIVEQAAIVGAFNPEAFATPEKAKKTADNVASRLDLIADDMERGWNG
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
EFTPNGDLYCERILRGVKDVITLDAGLINSVDARQIDRISQNLMETYSSPPLLHRKDKSE
CCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
RILGPMSLLESIFTNGKKGITLQRYKGLGEMNAEQLWETTLNPDARALLQVKINDATDAD
HHHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCHH
SLFSRLMGDEVEPRRAFIQDNALSVANLDI
HHHHHHHCCCCCHHHHHHHCCCCCEEECCC
>Mature Secondary Structure 
SDEITSQMEGDDYGAASIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEA
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
LAGHATLVNVTLHADGSCSVRDNGRGIPTDIHPTEGVSAAEVIMTQLHAGGKFDQNSYKV
HCCCEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGVSVVNALSVWLQLRIRRNGKIHEMSFTHGVADAPLKIVGDCGKESGSEIRFL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHEECCCCCCCCEEEEE
PSSETFTMVEFDFETLERRLRELAFLNSGVHILLVDQRHADIKSVELHYEGGLTEFVKYI
CCCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHH
DQSKKALLDHPIYITSEKDGMNVEVALWWNDSYHEKVLCFTNNIPQRDGGTHLVGFRSAL
HHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCEEEHHHHHH
TRQINGYAESSGIVKKEKVNLTGDDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPIV
HHHHCCHHHCCCCEEEEEECCCHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ENLVNEGLSAWLEEHPNEAKIVTSKVVEAAAAREAARKARELTRRKGALDITSLPGKLAD
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
CQERDPAKSEIFIVEGDSAGGSAKSGRSRQNQAILPLRGKILNVERARFDRMLSSDMIGT
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCEECHHHHHHHHHHHHHHHHH
LITALGTSIGKDEFSPDKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYI
HHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
AQPPLYKVTRGKSFQYIKNEAAFEEFLIDTGLEETTLELSTGEVRAGADLRQLAEDARVL
ECCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
RQLLNGLHTRYNRNIVEQAAIVGAFNPEAFATPEKAKKTADNVASRLDLIADDMERGWNG
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
EFTPNGDLYCERILRGVKDVITLDAGLINSVDARQIDRISQNLMETYSSPPLLHRKDKSE
CCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
RILGPMSLLESIFTNGKKGITLQRYKGLGEMNAEQLWETTLNPDARALLQVKINDATDAD
HHHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCHH
SLFSRLMGDEVEPRRAFIQDNALSVANLDI
HHHHHHHCCCCCHHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]