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The map label for this gene is malK [H]

Identifier: 49183571

GI number: 49183571

Start: 584690

End: 586294

Strand: Direct

Name: malK [H]

Synonym: BAS0545

Alternate gene names: 49183571

Gene position: 584690-586294 (Clockwise)

Preceding gene: 49183570

Following gene: 49183572

Centisome position: 11.18

GC content: 33.64

Gene sequence:

>1605_bases
ATGAAAAAAAGGAAAAGACTATGGAATTTATGGAAAACGATTACATTGTTAGTTTGTACAGTTGTAATTTTTTCTTTACT
TGTGACAGATATATTAATTAGTCACAATGTAGAACGGACAACGGAGGATAGCCAAGCAGAGAAAGCAAAAACAATTGCAC
ACATTGTGGCGAATGATTCAATTGTAATCGATGGTTTAATTGGGAAAGCAGATACTTCTGCAATTCAAACGTATACAAAT
AGAATATTAAAAAACACAGGTGTTCAATTTATTGTAGTTATGGACATGAATGGAATAAGAAAGTCACATCCAAATCCTCA
AAAAATAGGTCATCATTTTATTGGGGGAGATGAAGGACCTGCATTGAAAGGAAAGGAACATGTATCGCTGGCAGAAGGAA
CTTTAGGTATTTCCATGCGAGTGTTTGTACCAATATTTTCTGAAACAGGTGAACAACTTGGAGCAGTGGCTGTTGGTATT
TCAGCAGATAATGTAAAAGAGAGAGTTAAGGAAAGTAGACATATCATTTATATTGGTGTCGGAGTTGGTGTATTAGTCGG
AATTATAGGAGCAATATTGCTAGCTAGACATATAAAGAAAAGTTTATTCGGTCTTGAGCCGCACAGGATAGCAAAAATTC
TTGAAGAAAGAAATACGATGCTACAATCTGTAAAGGAAGGTATTATTGCTGTAGATAAAGAGGCTAGAGTAACGTTAATT
AATAATGAAGCGAAACGTCTATTTAAAAAAAGCGGACTTGAAGAAGATTTTATCGGTAAAGATGTTGAGTTGTATATGCC
AAATTCACGTATAAAAGAAGTATTGCAAACGGGAGAAGTACAATTAAACGAAGAACAAAATATTTACGGAATTACGATTG
TGACGAATCGAGTTCCTTTATATGTAAAAGAAGAAATAGTTGGTGCAATTGCAACATTTCGTGATAAAACAGAGATTAGA
AAACTAGCAGAGGAACTAACTGGTATTAGACTATATGCAGAAGCATTACGGGCACAATCTCATGAGTTTATGAATAAGAT
GCATGTCGTATTAGGGCTTACACATATGAAACAGTATGAAGAATTACAGAAGTATATAAGCGGCATGGTATCAGAGCATC
AATATGAAATTGGTGGAGTTATGAAAAGAATAAAAAGTCCAGTATTTGCTGGTTTTTTACTAGGTAAGCTCAGCTATGCT
AGAGAGAAAAATATAAAATTAATTATAAGTGAAGATTCTTATATGCCAGAAATAGATGATGAAAGTATTACTCATGAACT
TATTACGATTGTCGGAAATTTAATTGATAATGCATTAGAGGCAGTTACGAATTGTGAAAAGAAGCGAGTTGAAGTTAAGA
TACAACATGAGGATATACTAACTATTACAGTACAAGATACGGGAAAAGGTATACAAGAAAAAGAAATAGAGGAATTATTT
ACGAAAGGTTATTCCACAAAGGGAGATAACCGCGGTTATGGTTTGTATCTTGTAAAAGAAAGTATACAGCGAATAAATGG
GGAAATTCATATGCATTCATTAGTAGGTAAGGGAACAACGATAACAATTGAAATACCTAAAGGTAGGGATGAGAGGCAAA
TATGA

Upstream 100 bases:

>100_bases
GACTAGTAAATTTAAAGTATAATAACAGCAGATAAGTAAGGTGAGAATAATATCACCTTACTTATTTTTCTATTCAGCAA
TTAGGGGAGTCATTAACGGG

Downstream 100 bases:

>100_bases
TTAAAGTTTTAATTGTAGAAGATGACCCGATGGTAGCAATGCTAAATACGCATTATTTAGAGCAAGTAGGAGGATTTGAA
CTTGTTCAAGCAGTTAATTC

Product: sensory histidine kinase DcuS

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPIFSETGEQLGAVAVGI
SADNVKERVKESRHIIYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKEEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYA
REKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKRVEVKIQHEDILTITVQDTGKGIQEKEIEELF
TKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTTITIEIPKGRDERQI

Sequences:

>Translated_534_residues
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPIFSETGEQLGAVAVGI
SADNVKERVKESRHIIYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKEEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYA
REKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKRVEVKIQHEDILTITVQDTGKGIQEKEIEELF
TKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTTITIEIPKGRDERQI
>Mature_534_residues
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPIFSETGEQLGAVAVGI
SADNVKERVKESRHIIYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKEEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYA
REKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKRVEVKIQHEDILTITVQDTGKGIQEKEIEELF
TKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTTITIEIPKGRDERQI

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=526, Percent_Identity=40.3041825095057, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1786837, Length=527, Percent_Identity=30.7400379506641, Blast_Score=245, Evalue=7e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59849; Mature: 59849

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
IVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGP
EEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCC
ALKGKEHVSLAEGTLGISMRVFVPIFSETGEQLGAVAVGISADNVKERVKESRHIIYIGV
CCCCCCCCEEECCCCCEEEEEEEEEHHHCCHHHCEEEEECCHHHHHHHHHHCCCEEEEEC
GVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEE
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
CHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEECCCCCEEEEEEEECCCCE
YVKEEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYE
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIDD
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
ESITHELITIVGNLIDNALEAVTNCEKKRVEVKIQHEDILTITVQDTGKGIQEKEIEELF
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEEECCEEEEEEECCCCCCCHHHHHHHH
TKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTTITIEIPKGRDERQI
HCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
IVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGP
EEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCC
ALKGKEHVSLAEGTLGISMRVFVPIFSETGEQLGAVAVGISADNVKERVKESRHIIYIGV
CCCCCCCCEEECCCCCEEEEEEEEEHHHCCHHHCEEEEECCHHHHHHHHHHCCCEEEEEC
GVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEE
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
CHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEECCCCCEEEEEEEECCCCE
YVKEEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYE
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIDD
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
ESITHELITIVGNLIDNALEAVTNCEKKRVEVKIQHEDILTITVQDTGKGIQEKEIEELF
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEEECCEEEEEEECCCCCCCHHHHHHHH
TKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTTITIEIPKGRDERQI
HCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]