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The map label for this gene is dus [H]

Identifier: 46447621

GI number: 46447621

Start: 2368949

End: 2369842

Strand: Reverse

Name: dus [H]

Synonym: pc1987

Alternate gene names: 46447621

Gene position: 2369842-2368949 (Counterclockwise)

Preceding gene: 46447622

Following gene: 46447620

Centisome position: 98.15

GC content: 40.38

Gene sequence:

>894_bases
ATGGTAAAGATGGATGCTTTGATTCGCCACGATCCGCATACATATCATATTTTAGACTACGCAGCAAATATGCATCCGAT
TGGAGGGCAATTATGTGGAAGTAAGCCCTCAATTGCAGGACAAGCAGCTAAAATTATCGAAGAATTAGGATTTGACATTG
TTGATTTGAATTGCGGTTGTCCAGTTGACAAAATTACAAAAGATGGAAGTGGGTCGGGATTATTAAAAACGCCTGAATTA
ATTGGCGAGATCGTGTGTAATATGGTGTCGGCCGTCAAAATTCCCGTGACTGTAAAAATCAGAGCTGGATGGGACGAGCA
ACAGATTCAAGTTGAGAATATTGTTCAAATAGTGGAACAAGCGGGAGCCCAAGCAATTTGTATTCATGGGCGGACGCGCC
AGCAAGGATATCGTGGACCAGCTAATTGGGAATATATTAAGCGAGGAAAATTAGCCGCTAAATCGATTAAGATTATTGGA
AATGGTGATGTTTTAGATGGTCCATCGGCAGCAAAAATGTTTTGTGAAACAGGATGTGATGCCGTTTTAGTGGCAAGGGG
AACGTTGGGACAGCCTTGGATTGTTCAAGACATTCTACGTTATTTAGAAGGAAAAACTGCTATTTGTCATTCGTTGGAAG
ATTGCCGGCAAGCTCTTTACCAACATTTCTTAGAGACATTACATTATCATCCCGATCAACGGGTGTGTATAGATATGCGC
CGAGTCGGTTGTTGGTATTTAAAAAAATCATCTGGAACAAGGCTGTTTCGAGAATTGATTAGCAAAGCTTCTGACGTGGA
AGTCATTAAAAATTTAATTTTGGATTTCCCCTTAGGTGATTCGACTGAAAGTGAAGACATAGCTCAAACTCAAAAAGTTG
ATTGTTGTGCTTAA

Upstream 100 bases:

>100_bases
GCTTCCTAATCGAATTTTTTATGCACCTTTAGCTGGGTGTTCTGATTTTCCATTTCGTAAAATGTCTGCTCAATATAAAC
CAGGATTAATGTATTGCGAA

Downstream 100 bases:

>100_bases
TTAAGAAACGTTCATGCATAAGAGATTTAGCAGAGCTTAGGAAAATTGACTCAAATTGCAAAAACTATTTTTTAGCTTGC
TGAAGAAGGGACAATTAAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MVKMDALIRHDPHTYHILDYAANMHPIGGQLCGSKPSIAGQAAKIIEELGFDIVDLNCGCPVDKITKDGSGSGLLKTPEL
IGEIVCNMVSAVKIPVTVKIRAGWDEQQIQVENIVQIVEQAGAQAICIHGRTRQQGYRGPANWEYIKRGKLAAKSIKIIG
NGDVLDGPSAAKMFCETGCDAVLVARGTLGQPWIVQDILRYLEGKTAICHSLEDCRQALYQHFLETLHYHPDQRVCIDMR
RVGCWYLKKSSGTRLFRELISKASDVEVIKNLILDFPLGDSTESEDIAQTQKVDCCA

Sequences:

>Translated_297_residues
MVKMDALIRHDPHTYHILDYAANMHPIGGQLCGSKPSIAGQAAKIIEELGFDIVDLNCGCPVDKITKDGSGSGLLKTPEL
IGEIVCNMVSAVKIPVTVKIRAGWDEQQIQVENIVQIVEQAGAQAICIHGRTRQQGYRGPANWEYIKRGKLAAKSIKIIG
NGDVLDGPSAAKMFCETGCDAVLVARGTLGQPWIVQDILRYLEGKTAICHSLEDCRQALYQHFLETLHYHPDQRVCIDMR
RVGCWYLKKSSGTRLFRELISKASDVEVIKNLILDFPLGDSTESEDIAQTQKVDCCA
>Mature_297_residues
MVKMDALIRHDPHTYHILDYAANMHPIGGQLCGSKPSIAGQAAKIIEELGFDIVDLNCGCPVDKITKDGSGSGLLKTPEL
IGEIVCNMVSAVKIPVTVKIRAGWDEQQIQVENIVQIVEQAGAQAICIHGRTRQQGYRGPANWEYIKRGKLAAKSIKIIG
NGDVLDGPSAAKMFCETGCDAVLVARGTLGQPWIVQDILRYLEGKTAICHSLEDCRQALYQHFLETLHYHPDQRVCIDMR
RVGCWYLKKSSGTRLFRELISKASDVEVIKNLILDFPLGDSTESEDIAQTQKVDCCA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=173, Percent_Identity=32.9479768786127, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI239788462, Length=173, Percent_Identity=32.9479768786127, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI31742496, Length=170, Percent_Identity=31.7647058823529, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI40807366, Length=173, Percent_Identity=30.0578034682081, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI8923374, Length=155, Percent_Identity=34.1935483870968, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789660, Length=239, Percent_Identity=36.4016736401674, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1788462, Length=168, Percent_Identity=42.2619047619048, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI145693211, Length=118, Percent_Identity=35.5932203389831, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=184, Percent_Identity=27.7173913043478, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=172, Percent_Identity=29.6511627906977, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=180, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17510279, Length=177, Percent_Identity=30.5084745762712, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323433, Length=168, Percent_Identity=33.9285714285714, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=257, Percent_Identity=29.9610894941634, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=150, Percent_Identity=32.6666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24585320, Length=181, Percent_Identity=37.0165745856354, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24580595, Length=178, Percent_Identity=35.3932584269663, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920448, Length=178, Percent_Identity=35.3932584269663, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921524, Length=193, Percent_Identity=29.5336787564767, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI45549423, Length=213, Percent_Identity=30.9859154929577, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 32732; Mature: 32732

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKMDALIRHDPHTYHILDYAANMHPIGGQLCGSKPSIAGQAAKIIEELGFDIVDLNCGC
CCCCCHHHHCCCCEEEEEEHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCC
PVDKITKDGSGSGLLKTPELIGEIVCNMVSAVKIPVTVKIRAGWDEQQIQVENIVQIVEQ
CHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEECCEEEEEECCCCHHHHHHHHHHHHHHH
AGAQAICIHGRTRQQGYRGPANWEYIKRGKLAAKSIKIIGNGDVLDGPSAAKMFCETGCD
CCCCEEEEECCHHHCCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCC
AVLVARGTLGQPWIVQDILRYLEGKTAICHSLEDCRQALYQHFLETLHYHPDQRVCIDMR
EEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RVGCWYLKKSSGTRLFRELISKASDVEVIKNLILDFPLGDSTESEDIAQTQKVDCCA
HHCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MVKMDALIRHDPHTYHILDYAANMHPIGGQLCGSKPSIAGQAAKIIEELGFDIVDLNCGC
CCCCCHHHHCCCCEEEEEEHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCC
PVDKITKDGSGSGLLKTPELIGEIVCNMVSAVKIPVTVKIRAGWDEQQIQVENIVQIVEQ
CHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEECCEEEEEECCCCHHHHHHHHHHHHHHH
AGAQAICIHGRTRQQGYRGPANWEYIKRGKLAAKSIKIIGNGDVLDGPSAAKMFCETGCD
CCCCEEEEECCHHHCCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCC
AVLVARGTLGQPWIVQDILRYLEGKTAICHSLEDCRQALYQHFLETLHYHPDQRVCIDMR
EEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RVGCWYLKKSSGTRLFRELISKASDVEVIKNLILDFPLGDSTESEDIAQTQKVDCCA
HHCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]