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The map label for this gene is dnaN [H]

Identifier: 46447339

GI number: 46447339

Start: 2043622

End: 2044833

Strand: Direct

Name: dnaN [H]

Synonym: pc1705

Alternate gene names: 46447339

Gene position: 2043622-2044833 (Clockwise)

Preceding gene: 46447338

Following gene: 46447340

Centisome position: 84.64

GC content: 37.13

Gene sequence:

>1212_bases
ATGGAAGGGAAAAAGATCCTTGACACAGAGCAAGTAATCTCGTATCTTCAGTCACCAAACTTATCAGAAATAAAGAGGAT
TGAGATTATGAAATTTGTTATCTCCACTCAAGAACTTAACTATCTCATTAGCAAAATCCTAAATGTTGTTGCTCAAAAAC
CGACAATTCCTATTCTATCTAATTTTCTTATCGAAGCTTATAATGATGAATTAATTTTAACTGCTACTGACTTGACAGTG
GGCATTCACTGCCACACTGAAGCTAAAATTCTCGAAGAAGGATCTACGACACTTCCAGCTAAGCGTCTTTCTCAATTAGT
TCGAGAATTAACAGCAGTCAATGTAGAGATCTCTACTAATACCAATGAAATTACAACCATTATCGCTGGTAGCTCACGTT
TCAAATTAAATGGCATGAGTAAAACAGACTATCCATCACTCCCTGATCTTTCTCAATCTCATACATTTGAGATGAAACAA
AGTGAATTAAAAGATTTGCTTTATCGTACCTCTTTTGCTGTTTCAAAAGAAGATAATCGCTATGTGTTAACCGGTGTCTT
TATACAAATTGCTAATGGAAGTATCACTTTCATTGGAACAGACGGAAAGCGATTAGCTCGAGCAAAAGGCGTAATAGATA
ATGATCCCTCTTTCAGCGGGCAAGCCATTATTCCAATTAAAGCTGTGGACGAAATTCTTAAAAATTTAACAGATGAGGGG
GAAGTTAAAATTTCATTAATGGCAGATAAAGTGGCTATTGAAGCCAATCAAATGCGAATTTTAACCAAGCTCCTGTCGGG
TGATTATCCTGATGTCAACCGAGTGATTCCAGAAAAATCAGAAATTATTGTCACCCTTCATCGAGAAGAGTTGTCAAGCT
TGCTTCGACAAATTTCTCTTTTTACAGCAGACCATAATCACTCGGTTCGATTTACTTTTATTCCGGGAGAATTGAAACTA
ACAGCAAACACAATGGACATTGGAGAAGGTAATGTCAGCATGCCCGTCAATTATGATGGTCCGAAGCTCGAAATAGCCTT
TAATCCAGGTTTTTTTATTGATATCTTAAGACATTGCAAAGGAGAAACAGTCATCATGGGACTCACAGATGCCTACAATC
CTGGTATTATTACAGACAGCGCAGAATTAGCGAATTCATTAGACGCTTCTCCTCTTTTTGTAATTATGCCGATGCGCTTG
TCAGAAGATTAA

Upstream 100 bases:

>100_bases
AGTTTATCCACTAAAAACTAACCACCATTAACTTTTTGCGTATGTAGCTAACAAAAAATTTAATTTTGAAATTAGCACAT
ACGTAAATTGCGCTATTAGG

Downstream 100 bases:

>100_bases
ATAGAATTATGACCCTTCGCTCTCTGTACTTGCAACATTTTCGCAATTATGAAGAAGCTTATTTGGAATTTAGTCCTCAA
TTCAATTTAATTTGTGGGCC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MEGKKILDTEQVISYLQSPNLSEIKRIEIMKFVISTQELNYLISKILNVVAQKPTIPILSNFLIEAYNDELILTATDLTV
GIHCHTEAKILEEGSTTLPAKRLSQLVRELTAVNVEISTNTNEITTIIAGSSRFKLNGMSKTDYPSLPDLSQSHTFEMKQ
SELKDLLYRTSFAVSKEDNRYVLTGVFIQIANGSITFIGTDGKRLARAKGVIDNDPSFSGQAIIPIKAVDEILKNLTDEG
EVKISLMADKVAIEANQMRILTKLLSGDYPDVNRVIPEKSEIIVTLHREELSSLLRQISLFTADHNHSVRFTFIPGELKL
TANTMDIGEGNVSMPVNYDGPKLEIAFNPGFFIDILRHCKGETVIMGLTDAYNPGIITDSAELANSLDASPLFVIMPMRL
SED

Sequences:

>Translated_403_residues
MEGKKILDTEQVISYLQSPNLSEIKRIEIMKFVISTQELNYLISKILNVVAQKPTIPILSNFLIEAYNDELILTATDLTV
GIHCHTEAKILEEGSTTLPAKRLSQLVRELTAVNVEISTNTNEITTIIAGSSRFKLNGMSKTDYPSLPDLSQSHTFEMKQ
SELKDLLYRTSFAVSKEDNRYVLTGVFIQIANGSITFIGTDGKRLARAKGVIDNDPSFSGQAIIPIKAVDEILKNLTDEG
EVKISLMADKVAIEANQMRILTKLLSGDYPDVNRVIPEKSEIIVTLHREELSSLLRQISLFTADHNHSVRFTFIPGELKL
TANTMDIGEGNVSMPVNYDGPKLEIAFNPGFFIDILRHCKGETVIMGLTDAYNPGIITDSAELANSLDASPLFVIMPMRL
SED
>Mature_403_residues
MEGKKILDTEQVISYLQSPNLSEIKRIEIMKFVISTQELNYLISKILNVVAQKPTIPILSNFLIEAYNDELILTATDLTV
GIHCHTEAKILEEGSTTLPAKRLSQLVRELTAVNVEISTNTNEITTIIAGSSRFKLNGMSKTDYPSLPDLSQSHTFEMKQ
SELKDLLYRTSFAVSKEDNRYVLTGVFIQIANGSITFIGTDGKRLARAKGVIDNDPSFSGQAIIPIKAVDEILKNLTDEG
EVKISLMADKVAIEANQMRILTKLLSGDYPDVNRVIPEKSEIIVTLHREELSSLLRQISLFTADHNHSVRFTFIPGELKL
TANTMDIGEGNVSMPVNYDGPKLEIAFNPGFFIDILRHCKGETVIMGLTDAYNPGIITDSAELANSLDASPLFVIMPMRL
SED

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=29.7368421052632, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 44679; Mature: 44679

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGKKILDTEQVISYLQSPNLSEIKRIEIMKFVISTQELNYLISKILNVVAQKPTIPILS
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
NFLIEAYNDELILTATDLTVGIHCHTEAKILEEGSTTLPAKRLSQLVRELTAVNVEISTN
HHHHHHCCCCEEEEEECEEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHEEEEEEECC
TNEITTIIAGSSRFKLNGMSKTDYPSLPDLSQSHTFEMKQSELKDLLYRTSFAVSKEDNR
CCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCC
YVLTGVFIQIANGSITFIGTDGKRLARAKGVIDNDPSFSGQAIIPIKAVDEILKNLTDEG
EEEEEEEEEEECCEEEEEECCCHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCC
EVKISLMADKVAIEANQMRILTKLLSGDYPDVNRVIPEKSEIIVTLHREELSSLLRQISL
CEEEEEEEEEEEEEHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEHHHHHHHHHHHHH
FTADHNHSVRFTFIPGELKLTANTMDIGEGNVSMPVNYDGPKLEIAFNPGFFIDILRHCK
EEECCCCEEEEEEECCEEEEEEEEEEECCCCEEEEECCCCCEEEEEECCCHHHHHHHHCC
GETVIMGLTDAYNPGIITDSAELANSLDASPLFVIMPMRLSED
CCEEEEEECCCCCCCEEECCHHHHHCCCCCCEEEEEECEECCC
>Mature Secondary Structure
MEGKKILDTEQVISYLQSPNLSEIKRIEIMKFVISTQELNYLISKILNVVAQKPTIPILS
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
NFLIEAYNDELILTATDLTVGIHCHTEAKILEEGSTTLPAKRLSQLVRELTAVNVEISTN
HHHHHHCCCCEEEEEECEEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHEEEEEEECC
TNEITTIIAGSSRFKLNGMSKTDYPSLPDLSQSHTFEMKQSELKDLLYRTSFAVSKEDNR
CCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCC
YVLTGVFIQIANGSITFIGTDGKRLARAKGVIDNDPSFSGQAIIPIKAVDEILKNLTDEG
EEEEEEEEEEECCEEEEEECCCHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCC
EVKISLMADKVAIEANQMRILTKLLSGDYPDVNRVIPEKSEIIVTLHREELSSLLRQISL
CEEEEEEEEEEEEEHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEHHHHHHHHHHHHH
FTADHNHSVRFTFIPGELKLTANTMDIGEGNVSMPVNYDGPKLEIAFNPGFFIDILRHCK
EEECCCCEEEEEEECCEEEEEEEEEEECCCCEEEEECCCCCEEEEEECCCHHHHHHHHCC
GETVIMGLTDAYNPGIITDSAELANSLDASPLFVIMPMRLSED
CCEEEEEECCCCCCCEEECCHHHHHCCCCCCEEEEEECEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]