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The map label for this gene is ispH

Identifier: 46446712

GI number: 46446712

Start: 1288939

End: 1289880

Strand: Reverse

Name: ispH

Synonym: pc1078

Alternate gene names: 46446712

Gene position: 1289880-1288939 (Counterclockwise)

Preceding gene: 46446713

Following gene: 46446711

Centisome position: 53.42

GC content: 38.32

Gene sequence:

>942_bases
ATGAAACTGTTGTTATCTAAGCCCCGCGGTTTTTGTGCTGGAGTCGAGCGAGCGATTGAAACAGTTGAAAAAGCTCTTCT
TCTTTGGGGATCTCCTATTTATGTCAAGCATGAGATTGTTCACAATCGACATGTTGTTCAAGGCTTAAAGACGAAAGGAG
CTATTTTTATTGAAGATGTAGAAGATGTACCAGTGGGAGCTAGGTTGATTTATTCAGCACATGGTGTATCACCTGCGGTC
AGAGAACAAGCTAAAAAAAGACAACTGATAGAAATAGACGCCACATGTGGCTTAGTAACTAGAGTTCATTCTGCAGTAAA
ACGATATGCTTTACAAGGCTATCAGATTATCTTGATTGGCCATCATAACCATGTCGAGATTATTGGAACCGCAGGTGAAG
CACCTGATGTAACAACAATCGTAGAGTCTGTGGAAGATGTCAAAAATTTAAATTTCTCTTCTCAGGACAAACTTTTTTAT
ATTACACAAACAACTTTGAGTTTGGATGATGTGAAAGAGATTACGCAAGCCCTAATTGTTAAATATCCTCATGTAGAAAC
ATTACCGAGTTCTTCCATTTGCTATGCGACAACAAACCGCCAAATGGCCCTTAGGGAAATTACTGACCTAACAGATCTTG
TGTTAGTCGTGGGTGATCCGCAAAGTTCCAATTCAAATCGATTGAGAGAAGCTGCTTCGACAAGAGGAATAGAATCTTAT
TTAATTAATGATGAAAAAGAAATTCATCCTGAATGGCTATTAGGAAAGCAAGTCATAGGCTTGACTGCTGGTGCATCAAC
TCCTGAAGAAATTGTTCAAAAATGCATCCAGTGTTTGATAGAATTAGGTGTAAATGAAGTGGAAGACATCGTTTATACTC
ACGAAGATGTTGTCTTCCAATTACCAAAACCTATTGTTAACGCACGTTTTAGTGTTGATTAA

Upstream 100 bases:

>100_bases
CATATTCGAGTGAAAGAAGAAAAAAAATGATAAGGATTGTCAAATACTCCCATTTTCTTTATGATTCCCCTTCTAATTTC
AAGAGATGTTTAGGTTGAAA

Downstream 100 bases:

>100_bases
TTCTAGAATTTATTGTTAAAGTGTATTTTTTTAAGATCCCTGAACCATTAAAAATCATTAAGAGGACATATATGCGCAAA
ATCCTTTTGACAATTATGGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKLLLSKPRGFCAGVERAIETVEKALLLWGSPIYVKHEIVHNRHVVQGLKTKGAIFIEDVEDVPVGARLIYSAHGVSPAV
REQAKKRQLIEIDATCGLVTRVHSAVKRYALQGYQIILIGHHNHVEIIGTAGEAPDVTTIVESVEDVKNLNFSSQDKLFY
ITQTTLSLDDVKEITQALIVKYPHVETLPSSSICYATTNRQMALREITDLTDLVLVVGDPQSSNSNRLREAASTRGIESY
LINDEKEIHPEWLLGKQVIGLTAGASTPEEIVQKCIQCLIELGVNEVEDIVYTHEDVVFQLPKPIVNARFSVD

Sequences:

>Translated_313_residues
MKLLLSKPRGFCAGVERAIETVEKALLLWGSPIYVKHEIVHNRHVVQGLKTKGAIFIEDVEDVPVGARLIYSAHGVSPAV
REQAKKRQLIEIDATCGLVTRVHSAVKRYALQGYQIILIGHHNHVEIIGTAGEAPDVTTIVESVEDVKNLNFSSQDKLFY
ITQTTLSLDDVKEITQALIVKYPHVETLPSSSICYATTNRQMALREITDLTDLVLVVGDPQSSNSNRLREAASTRGIESY
LINDEKEIHPEWLLGKQVIGLTAGASTPEEIVQKCIQCLIELGVNEVEDIVYTHEDVVFQLPKPIVNARFSVD
>Mature_313_residues
MKLLLSKPRGFCAGVERAIETVEKALLLWGSPIYVKHEIVHNRHVVQGLKTKGAIFIEDVEDVPVGARLIYSAHGVSPAV
REQAKKRQLIEIDATCGLVTRVHSAVKRYALQGYQIILIGHHNHVEIIGTAGEAPDVTTIVESVEDVKNLNFSSQDKLFY
ITQTTLSLDDVKEITQALIVKYPHVETLPSSSICYATTNRQMALREITDLTDLVLVVGDPQSSNSNRLREAASTRGIESY
LINDEKEIHPEWLLGKQVIGLTAGASTPEEIVQKCIQCLIELGVNEVEDIVYTHEDVVFQLPKPIVNARFSVD

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=308, Percent_Identity=48.3766233766234, Blast_Score=293, Evalue=9e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_PARUW (Q6MC97)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008077.1
- STRING:   Q6MC97
- GeneID:   2780649
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1078
- NMPDR:   fig|264201.1.peg.1078
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   Q6MC97
- ProtClustDB:   PRK01045
- BioCyc:   CPRO264201:PC1078-MONOMER
- BioCyc:   PCHL-E25-01:PCHL-E25-01-001078-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34708; Mature: 34708

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLLSKPRGFCAGVERAIETVEKALLLWGSPIYVKHEIVHNRHVVQGLKTKGAIFIEDV
CCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCEEEECC
EDVPVGARLIYSAHGVSPAVREQAKKRQLIEIDATCGLVTRVHSAVKRYALQGYQIILIG
CCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHCCEEEEEEE
HHNHVEIIGTAGEAPDVTTIVESVEDVKNLNFSSQDKLFYITQTTLSLDDVKEITQALIV
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHHHHHHHHHHH
KYPHVETLPSSSICYATTNRQMALREITDLTDLVLVVGDPQSSNSNRLREAASTRGIESY
CCCCCCCCCCCCEEEEECCCHHHHHHHHCHHEEEEEEECCCCCCHHHHHHHHHHCCCHHH
LINDEKEIHPEWLLGKQVIGLTAGASTPEEIVQKCIQCLIELGVNEVEDIVYTHEDVVFQ
HCCCCHHCCHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHEE
LPKPIVNARFSVD
CCCHHHCCCCCCC
>Mature Secondary Structure
MKLLLSKPRGFCAGVERAIETVEKALLLWGSPIYVKHEIVHNRHVVQGLKTKGAIFIEDV
CCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCEEEECC
EDVPVGARLIYSAHGVSPAVREQAKKRQLIEIDATCGLVTRVHSAVKRYALQGYQIILIG
CCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHCCEEEEEEE
HHNHVEIIGTAGEAPDVTTIVESVEDVKNLNFSSQDKLFYITQTTLSLDDVKEITQALIV
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHHHHHHHHHHH
KYPHVETLPSSSICYATTNRQMALREITDLTDLVLVVGDPQSSNSNRLREAASTRGIESY
CCCCCCCCCCCCEEEEECCCHHHHHHHHCHHEEEEEEECCCCCCHHHHHHHHHHCCCHHH
LINDEKEIHPEWLLGKQVIGLTAGASTPEEIVQKCIQCLIELGVNEVEDIVYTHEDVVFQ
HCCCCHHCCHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHEE
LPKPIVNARFSVD
CCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA