Click here to switch to the map view.

The map label for this gene is gyrA [H]

Identifier: 46446708

GI number: 46446708

Start: 1281957

End: 1284527

Strand: Reverse

Name: gyrA [H]

Synonym: pc1074

Alternate gene names: 46446708

Gene position: 1284527-1281957 (Counterclockwise)

Preceding gene: 46446709

Following gene: 46446707

Centisome position: 53.2

GC content: 39.52

Gene sequence:

>2571_bases
ATGTCCTACACTAAAGACGAAGTCATCGTTTCTCGAAACGTAGAAGATGAAATGAAAGATAGCTATTTACGCTATTCAAT
GTCAGTCATCATTTCTCGCGCTCTTCCGGATGCACGTGATGGTTTAAAACCCTCTCAGCGTCGAATTCTCTATGCTATGC
GACAACTTAATCTAGGTCCTAATGGAAAGCATAGAAAATGTGCCAAAATTTCGGGTGACACGTCGGGTGACTACCATCCA
CATGGAGAAATGGTCATTTACCCAACTCTCGTTAGAATGGCTCAGAAGTGGATTATGCGATACAATTTGGTAGATGGACA
AGGAAACTTCGGTTCTGTTGATGGAGATCCTCCGGCTGCGATGCGTTATACTGAAGCTCGTTTGACAACAGCAGCTGTCC
AACTGATGGACGACTTGGATAAAGACACTGTTGAAATGGTTCCTAACTATGATGAGACTAAGAAAGAACCTACTGTTTTT
CCTTCTAAATTTCCTAATCTTCTTTGCAACGGTTCATCTGGAATTGCGGTGGGGATGGCTACGAATATTCCTCCTCACAA
TTTAAATGAGTTAATCAAAGCAACTTTACTCTTGTTAGAAAATCCTACAACTTCAATTGATGACATTATGAAGGTGATGC
CCGGCCCAGATTTTCCAACAGGAGGGATGATTTGCGGTTATCGGGGGATTAAAGAAGCTTTCCATACAGGAAGGGGAAAG
CTCATTTTAAGAGGAGTCATTCGAGTTGAGGAAAACGAAGATAATCCGGATCGTTCTCGTTTAGCCATCGATGAAATTCC
CTACAATGTAAATAAATCCCGTTTAATCGAACACATTGCAGAACTCATCAATAATAAAACGATTACAGGGATTTCAGATT
TACGCGATGAGTCCGATAAAGATGGAATGCGTATTGTTATCGAATTAAAGCGTGGAGAAGTTCCAGAAGTTATCATCAAT
CAGCTTTATAAGTTTAGTGATTTGCAAATTACCTTTGGATGCAACATGCTTGCATTAGATAAAGGTTTACCTCGTACCAT
GAATGTTAAACAGCTAATTTCTGTTTGGATTGAACATCGCATTGATGTGGTACGTCGACGTACTCGATTTGAGCTTAATA
AAGCAGAAGCGCGAGCTCATATTCTAGAAGGATATTTAAAAGCAATTGATCATTTAGATGAAGTTGTTCGTCTGATTCGT
GCAAGTAGTAATCGGGATGAGGCTCGCATTGAATTAATAAAACAATTTGAATTCACTGAACGTCAAGCCAATGCAATTTT
AGATTTAAGACTATATCAATTAACTGGGCTTGAAAGAGATAAAATTAATGATGAATATCAAGAATTGTTAAAAAAGATTG
ATTATTTACGAGCCGTTTTAGCAAGCGAAGCAATGGTTAAAGAGATTATTCGTGAGGAGCTATTAGATATTCAAAAGCAT
CATAAATCTGAGAGACAGACACAAATCATTGCGGCAGAAACTGAAGTCAATATGGAAGACTTGATCGCAAATGAGCCTGT
GATTATTACAATTTCTCAAGATGATTATATTAAGCGTATGCCTGTCAATACATTTCGTGAGCAACGAAGAGGTGGCCAGG
GTGTGACAGGAATGCAGCTCAAGCGAGAAGAAGATGTGATTAAGGGTCTATACGTTGCAACAACGCACGATTATTTGCTT
ATTTTCACTAATCTAGGTCGTTGTTATTGGTTAAAAGTATGGCAAATTCCAGAAACGGGACGCAAATCCAAAGGAAAACC
TCTTATTAATCTTTTAGAAGATATTCGCCCGGAAGAAAAAATTGCGACTATTCAACGCGTCTCTTCTTTTGAAGAAGAAG
CGTGCATTTTGATGGCTACTAAGCGTGCTGTTGTTAAAAAATCGCTTCTCAATGAATTTAGCAACCCCAGAAGAAAAGGG
ATTTGGGCTCTTGACATTGATGAAGGAGACGAACTTGTTGCCGCTCGCCTTGTTAAACCTGAGCAGCAAGTAATGCTTTT
TACTCATCAGGGGATGGCAGTCCGTTTTGAAGAAAGCAACGTTCGCCCAATGGGAAGAACAGCACGCGGAGTCAAAGGGG
CAACCTTACGTGGTGAAAATGATTATATCGTAGGATGTGAAGTTGTTAACGGTGATGAGTCTATCTTAGTCGTCTGTGAA
AATGGCTTCGGTAAACGTTCTCAGGTAGATGATTTCCGCCAAACAAATCGAGGTGGTGTAGGAGTTCGCTCTATTATTAC
AAGTGAACGTAATGGGAAGGTAATCGGAGCTCTCTGTGTCACAGATGAAGATGGCATGGTTATGATGTCTGCAACCGGGC
AAACAGTTCGTATTAATATGAATGATCTTCGGGTCATGGGGCGCAATACGCAAGGCGTCAAATTAGCAAATTTAAAAGAG
AATGATTATCTTGTCGCTATTCAGAAATTACAAGGTAGTGAAAATCAAAGTGAAGAGTTATTAGCTTCAGATACAATTGA
ACTTAACGAAAACAACGTTTCTTTTGAATCACCAGAAATTAAAATGTCAGTAAAGCCCAGCTCAGAAGAAATTACTGATC
AATCGGAATAG

Upstream 100 bases:

>100_bases
TTTAGATGTTTAATCGTGAATCAGTTCACTTTCTTTTAGTCACAAAACTTGTTGATGCGAGTGATAAAGGAATTTTGCAA
CTGGCCTAAATAGAGGTTAT

Downstream 100 bases:

>100_bases
ACATGCCTTCAACGAAATTTAAGGGGTATTTTATCACAATCGAAGGAGGAGAGGGATCTGGTAAATCGACCCTTCTCAAC
CAATTAGGAGACTATTTTCG

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 856; Mature: 855

Protein sequence:

>856_residues
MSYTKDEVIVSRNVEDEMKDSYLRYSMSVIISRALPDARDGLKPSQRRILYAMRQLNLGPNGKHRKCAKISGDTSGDYHP
HGEMVIYPTLVRMAQKWIMRYNLVDGQGNFGSVDGDPPAAMRYTEARLTTAAVQLMDDLDKDTVEMVPNYDETKKEPTVF
PSKFPNLLCNGSSGIAVGMATNIPPHNLNELIKATLLLLENPTTSIDDIMKVMPGPDFPTGGMICGYRGIKEAFHTGRGK
LILRGVIRVEENEDNPDRSRLAIDEIPYNVNKSRLIEHIAELINNKTITGISDLRDESDKDGMRIVIELKRGEVPEVIIN
QLYKFSDLQITFGCNMLALDKGLPRTMNVKQLISVWIEHRIDVVRRRTRFELNKAEARAHILEGYLKAIDHLDEVVRLIR
ASSNRDEARIELIKQFEFTERQANAILDLRLYQLTGLERDKINDEYQELLKKIDYLRAVLASEAMVKEIIREELLDIQKH
HKSERQTQIIAAETEVNMEDLIANEPVIITISQDDYIKRMPVNTFREQRRGGQGVTGMQLKREEDVIKGLYVATTHDYLL
IFTNLGRCYWLKVWQIPETGRKSKGKPLINLLEDIRPEEKIATIQRVSSFEEEACILMATKRAVVKKSLLNEFSNPRRKG
IWALDIDEGDELVAARLVKPEQQVMLFTHQGMAVRFEESNVRPMGRTARGVKGATLRGENDYIVGCEVVNGDESILVVCE
NGFGKRSQVDDFRQTNRGGVGVRSIITSERNGKVIGALCVTDEDGMVMMSATGQTVRINMNDLRVMGRNTQGVKLANLKE
NDYLVAIQKLQGSENQSEELLASDTIELNENNVSFESPEIKMSVKPSSEEITDQSE

Sequences:

>Translated_856_residues
MSYTKDEVIVSRNVEDEMKDSYLRYSMSVIISRALPDARDGLKPSQRRILYAMRQLNLGPNGKHRKCAKISGDTSGDYHP
HGEMVIYPTLVRMAQKWIMRYNLVDGQGNFGSVDGDPPAAMRYTEARLTTAAVQLMDDLDKDTVEMVPNYDETKKEPTVF
PSKFPNLLCNGSSGIAVGMATNIPPHNLNELIKATLLLLENPTTSIDDIMKVMPGPDFPTGGMICGYRGIKEAFHTGRGK
LILRGVIRVEENEDNPDRSRLAIDEIPYNVNKSRLIEHIAELINNKTITGISDLRDESDKDGMRIVIELKRGEVPEVIIN
QLYKFSDLQITFGCNMLALDKGLPRTMNVKQLISVWIEHRIDVVRRRTRFELNKAEARAHILEGYLKAIDHLDEVVRLIR
ASSNRDEARIELIKQFEFTERQANAILDLRLYQLTGLERDKINDEYQELLKKIDYLRAVLASEAMVKEIIREELLDIQKH
HKSERQTQIIAAETEVNMEDLIANEPVIITISQDDYIKRMPVNTFREQRRGGQGVTGMQLKREEDVIKGLYVATTHDYLL
IFTNLGRCYWLKVWQIPETGRKSKGKPLINLLEDIRPEEKIATIQRVSSFEEEACILMATKRAVVKKSLLNEFSNPRRKG
IWALDIDEGDELVAARLVKPEQQVMLFTHQGMAVRFEESNVRPMGRTARGVKGATLRGENDYIVGCEVVNGDESILVVCE
NGFGKRSQVDDFRQTNRGGVGVRSIITSERNGKVIGALCVTDEDGMVMMSATGQTVRINMNDLRVMGRNTQGVKLANLKE
NDYLVAIQKLQGSENQSEELLASDTIELNENNVSFESPEIKMSVKPSSEEITDQSE
>Mature_855_residues
SYTKDEVIVSRNVEDEMKDSYLRYSMSVIISRALPDARDGLKPSQRRILYAMRQLNLGPNGKHRKCAKISGDTSGDYHPH
GEMVIYPTLVRMAQKWIMRYNLVDGQGNFGSVDGDPPAAMRYTEARLTTAAVQLMDDLDKDTVEMVPNYDETKKEPTVFP
SKFPNLLCNGSSGIAVGMATNIPPHNLNELIKATLLLLENPTTSIDDIMKVMPGPDFPTGGMICGYRGIKEAFHTGRGKL
ILRGVIRVEENEDNPDRSRLAIDEIPYNVNKSRLIEHIAELINNKTITGISDLRDESDKDGMRIVIELKRGEVPEVIINQ
LYKFSDLQITFGCNMLALDKGLPRTMNVKQLISVWIEHRIDVVRRRTRFELNKAEARAHILEGYLKAIDHLDEVVRLIRA
SSNRDEARIELIKQFEFTERQANAILDLRLYQLTGLERDKINDEYQELLKKIDYLRAVLASEAMVKEIIREELLDIQKHH
KSERQTQIIAAETEVNMEDLIANEPVIITISQDDYIKRMPVNTFREQRRGGQGVTGMQLKREEDVIKGLYVATTHDYLLI
FTNLGRCYWLKVWQIPETGRKSKGKPLINLLEDIRPEEKIATIQRVSSFEEEACILMATKRAVVKKSLLNEFSNPRRKGI
WALDIDEGDELVAARLVKPEQQVMLFTHQGMAVRFEESNVRPMGRTARGVKGATLRGENDYIVGCEVVNGDESILVVCEN
GFGKRSQVDDFRQTNRGGVGVRSIITSERNGKVIGALCVTDEDGMVMMSATGQTVRINMNDLRVMGRNTQGVKLANLKEN
DYLVAIQKLQGSENQSEELLASDTIELNENNVSFESPEIKMSVKPSSEEITDQSE

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=318, Percent_Identity=25.7861635220126, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI19913406, Length=319, Percent_Identity=24.7648902821317, Blast_Score=85, Evalue=4e-16,
Organism=Escherichia coli, GI1788562, Length=876, Percent_Identity=46.4611872146119, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=673, Percent_Identity=35.0668647845468, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI212645657, Length=167, Percent_Identity=31.7365269461078, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535065, Length=168, Percent_Identity=31.547619047619, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI212645845, Length=385, Percent_Identity=25.1948051948052, Blast_Score=75, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=344, Percent_Identity=25.8720930232558, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17136538, Length=362, Percent_Identity=25.1381215469613, Blast_Score=81, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 96948; Mature: 96816

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTKDEVIVSRNVEDEMKDSYLRYSMSVIISRALPDARDGLKPSQRRILYAMRQLNLGP
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCC
NGKHRKCAKISGDTSGDYHPHGEMVIYPTLVRMAQKWIMRYNLVDGQGNFGSVDGDPPAA
CCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHH
MRYTEARLTTAAVQLMDDLDKDTVEMVPNYDETKKEPTVFPSKFPNLLCNGSSGIAVGMA
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEE
TNIPPHNLNELIKATLLLLENPTTSIDDIMKVMPGPDFPTGGMICGYRGIKEAFHTGRGK
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEECHHHHHHHHHCCCCC
LILRGVIRVEENEDNPDRSRLAIDEIPYNVNKSRLIEHIAELINNKTITGISDLRDESDK
EEEEEEEEEECCCCCCCHHCEEHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCC
DGMRIVIELKRGEVPEVIINQLYKFSDLQITFGCNMLALDKGLPRTMNVKQLISVWIEHR
CCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHH
IDVVRRRTRFELNKAEARAHILEGYLKAIDHLDEVVRLIRASSNRDEARIELIKQFEFTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RQANAILDLRLYQLTGLERDKINDEYQELLKKIDYLRAVLASEAMVKEIIREELLDIQKH
HHHHHEEEEEEHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HKSERQTQIIAAETEVNMEDLIANEPVIITISQDDYIKRMPVNTFREQRRGGQGVTGMQL
HCHHHHHEEEEEECCCCHHHHHCCCCEEEEECCCCCHHHCCHHHHHHHHCCCCCCCCCEE
KREEDVIKGLYVATTHDYLLIFTNLGRCYWLKVWQIPETGRKSKGKPLINLLEDIRPEEK
CCHHHHHCEEEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCHHH
IATIQRVSSFEEEACILMATKRAVVKKSLLNEFSNPRRKGIWALDIDEGDELVAARLVKP
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCHHCCEEEEECCCCCHHHHHHHCCC
EQQVMLFTHQGMAVRFEESNVRPMGRTARGVKGATLRGENDYIVGCEVVNGDESILVVCE
CCEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCEEECCCCEEEEEEEECCCCEEEEEEC
NGFGKRSQVDDFRQTNRGGVGVRSIITSERNGKVIGALCVTDEDGMVMMSATGQTVRINM
CCCCCCCHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCCEEEEEH
NDLRVMGRNTQGVKLANLKENDYLVAIQKLQGSENQSEELLASDTIELNENNVSFESPEI
HHEEEECCCCCCEEEECCCCCCEEEEEEECCCCCCCHHHHHHCCCEEECCCCCCCCCCCE
KMSVKPSSEEITDQSE
EEEECCCCCCCCCCCC
>Mature Secondary Structure 
SYTKDEVIVSRNVEDEMKDSYLRYSMSVIISRALPDARDGLKPSQRRILYAMRQLNLGP
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCC
NGKHRKCAKISGDTSGDYHPHGEMVIYPTLVRMAQKWIMRYNLVDGQGNFGSVDGDPPAA
CCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHH
MRYTEARLTTAAVQLMDDLDKDTVEMVPNYDETKKEPTVFPSKFPNLLCNGSSGIAVGMA
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEE
TNIPPHNLNELIKATLLLLENPTTSIDDIMKVMPGPDFPTGGMICGYRGIKEAFHTGRGK
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEECHHHHHHHHHCCCCC
LILRGVIRVEENEDNPDRSRLAIDEIPYNVNKSRLIEHIAELINNKTITGISDLRDESDK
EEEEEEEEEECCCCCCCHHCEEHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCC
DGMRIVIELKRGEVPEVIINQLYKFSDLQITFGCNMLALDKGLPRTMNVKQLISVWIEHR
CCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHH
IDVVRRRTRFELNKAEARAHILEGYLKAIDHLDEVVRLIRASSNRDEARIELIKQFEFTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RQANAILDLRLYQLTGLERDKINDEYQELLKKIDYLRAVLASEAMVKEIIREELLDIQKH
HHHHHEEEEEEHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HKSERQTQIIAAETEVNMEDLIANEPVIITISQDDYIKRMPVNTFREQRRGGQGVTGMQL
HCHHHHHEEEEEECCCCHHHHHCCCCEEEEECCCCCHHHCCHHHHHHHHCCCCCCCCCEE
KREEDVIKGLYVATTHDYLLIFTNLGRCYWLKVWQIPETGRKSKGKPLINLLEDIRPEEK
CCHHHHHCEEEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCHHH
IATIQRVSSFEEEACILMATKRAVVKKSLLNEFSNPRRKGIWALDIDEGDELVAARLVKP
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCHHCCEEEEECCCCCHHHHHHHCCC
EQQVMLFTHQGMAVRFEESNVRPMGRTARGVKGATLRGENDYIVGCEVVNGDESILVVCE
CCEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCEEECCCCEEEEEEEECCCCEEEEEEC
NGFGKRSQVDDFRQTNRGGVGVRSIITSERNGKVIGALCVTDEDGMVMMSATGQTVRINM
CCCCCCCHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCCEEEEEH
NDLRVMGRNTQGVKLANLKENDYLVAIQKLQGSENQSEELLASDTIELNENNVSFESPEI
HHEEEECCCCCCEEEECCCCCCEEEEEEECCCCCCCHHHHHHCCCEEECCCCCCCCCCCE
KMSVKPSSEEITDQSE
EEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9784136 [H]