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The map label for this gene is gyrB [H]

Identifier: 46446709

GI number: 46446709

Start: 1284615

End: 1287122

Strand: Reverse

Name: gyrB [H]

Synonym: pc1075

Alternate gene names: 46446709

Gene position: 1287122-1284615 (Counterclockwise)

Preceding gene: 46446710

Following gene: 46446708

Centisome position: 53.31

GC content: 37.6

Gene sequence:

>2508_bases
ATGTCTCAAGATACACTTAGTTCAGATAAACCCAAGATTCAACTCAAAGAGTACGATGCTAGCTCCATCACTGTTTTAGA
AGGACTTCAGGCTGTTCGAGAACGCCCTGGTATGTATATTGGTGATACAGGTGTCAATGGTCTTCATCATCTTGTCTATG
AAGTAATAGACAATTGTATAGATGAAGCAATGGCTGGTTACTGTACAGCTATCGACGTTGTTTTACATAAAGATAATTCT
ATTAGTATTGAAGACGATGGACGAGGGATTCCAGTTGAACGCCATGAGAATGAGTCTCGCAAGCAAGGAAGAGAAGTGTC
TGCCATAGAAGTTGTTATGACTATTCTGCATGCCGGGGGTAAGTTTGATAAAGATACTTATAAGGTTTCAGGAGGCTTGC
ATGGCGTCGGTGTTTCTTGTGTGAATGCTCTTTCAAAAAAAATGATTGTGCAAGTTTGTAAAAATGGCCTTATGCACGAA
ATTGAATTTTCTCAAGGGAAGGTTGTTAAACCAGTTCAAGTAATTGGAGAAACGACAAAACGAGGAACCAAAGTTTGGTT
TTGGCCTGATCCTGAAGTGATGACAACAATTGACTTCGACTATGACATTTTAGCTAAGCGATTCAGAGAATTAGCTTTTT
TGAATAAAGGAATTAATATTTTTTTTAGAGATGAAAAACATGCTGAAAAAGAAGATGTCAATTTTTGCTATCAAGGGGGG
TTAAGCTCATTTGTTTCTTACCTCAATGAAAATAAAGAACCTCTTTTTTCTAATCCTATTTATTTTCATGGGGTAAAGCC
TGGAGATGATGCTCCGATTGAATTTGAAGTTGCAATGCAATGGAATGATGGATATTCCGAAACCATTTTTTCTTATGTAA
ATAATATTCCAACAAGGCAAGGTGGGTCCCATTTAACTGGTTTTTCTACAGCTCTTACCCGTGTTTTGAATAATTACATT
AAAAATCATAACCTTTTGAAGTCGGATAAAATCTCCATTAACGGAGAAGATATGAGAGAGGGATTGACAGCTGTAATTTC
TGTCAAGGTCGCTAATCCTCAATTTGAAGGACAAACAAAACAAAGACTTGGTAATAGTGATGTAGGTTCTGTTGTCCAGC
AAATTGTCGGAGAAGAGTTAGCGATATTTCTCGATGAAAATCCTTCCATGGCTAAATTAATTACTGATAAAGCTATCATA
GCGGCGCAGGCACGTGAAGCTGCTCGCAAAGCAAGGGAATTAACTTTACGTAAATCGGCTTTGGATAGTGCGCGACTTCC
TGGAAAATTGACTGACTGCCAAGAAAAAAATCCAGCTCTTTGTGAAATTTATATTGTTGAGGGGGATTCTGCGGGTGGTT
CTGCTAAATCTGGAAGAGATAGGCGTTTTCAAGCAATCTTACCGATTCGAGGAAAAATTCTAAATGTTGAAAAAGCACGT
TTAGAAAAAGTATTACAAAATACTGAAGTAGGAACCATGGTGGCTGCTCTTGGTTGCGGGATTGGGAAAGACGGCTTTAA
TCTGGAAAAACTTCGTTATCATAAAGTCATTATCATGACGGATGCGGATGTGGACGGATCTCACATTCGTACACTCCTTT
TAACTTTTTTTTATCGTCATATGCCGGCGTTGGTAGAAAACAATTTTATTTATATTGCTCAACCTCCTCTTTATCGTGTG
TCCCGTAAAAAAACGAGTCGTTACATACACTCGGAAAGAGAAATGGATGATTATTTACTAGAGCTTGGATTAAGCGATAT
ACAAATAAAGCTAGCTTCCCGAGAAAATATTTTAAGTCTGGAAGAAACTAAAAAACTAGTTTCTACAATTTTGGCTGTTG
AGATTTTTATAGATCGCATAGAAAAAAAAGGAATTCCTTTTAGAGAATTTTTAGCTTTAAAAAATGAGAACGGTCTACTT
CCTCGTTTTCAAATGAATTTAATGGAGGGAGCTCGTTTTGCTTATTCAGAAGATGAATTTGTAGCTTTGAGACAGTCTGA
AGAAGAGAGTCAGCGTCAACGACATCAAGAAACGCTAGCTTCCATTCCAGCAGATGAAATTACTCCTGAAATGCGTACTT
TTAAACCTAGTCGACTTCATTTTATTGAGCTTTATGAAGAAGGTGGAATTGAAGAAATTCAAGAACCTTTACAAGAATAT
GGACTGGATTTTAGTTACTACATGTGTGCAAATGGAGTGATCTTGGACATTGTGGAAGAAGGGGGAAAAACTTACACTTA
TTACACACTACGCGAAGTCATTGATTTTTTGCGAAATAATGGACGTAAGGGAATTGAAATTCAGCGTTACAAAGGTCTTG
GTGAGATGAATGCGGACCAGCTTTGGGAAACCACGATGGATCCTGTTAAGAGAACTCTCATTAAAGTGACGTTACCGGAT
GTAATTGCAGCCGATCACATGTTTACGATGTTAATGGGAGAAGATGTTCCTCCCCGACGTGCTTTTATTGAACAACATGC
TCTGTCAGTTAAAAATTTAGATGTTTAA

Upstream 100 bases:

>100_bases
TAAGCCAGCTTAGACAAAAGTTTCCTCGAGTAGAAATCAAAACGATTTTATTTCGAATTGGTTGATACAGAGATAAAGTT
TCAATATTTAAGGTAATCGA

Downstream 100 bases:

>100_bases
TCGTGAATCAGTTCACTTTCTTTTAGTCACAAAACTTGTTGATGCGAGTGATAAAGGAATTTTGCAACTGGCCTAAATAG
AGGTTATATGTCCTACACTA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 835; Mature: 834

Protein sequence:

>835_residues
MSQDTLSSDKPKIQLKEYDASSITVLEGLQAVRERPGMYIGDTGVNGLHHLVYEVIDNCIDEAMAGYCTAIDVVLHKDNS
ISIEDDGRGIPVERHENESRKQGREVSAIEVVMTILHAGGKFDKDTYKVSGGLHGVGVSCVNALSKKMIVQVCKNGLMHE
IEFSQGKVVKPVQVIGETTKRGTKVWFWPDPEVMTTIDFDYDILAKRFRELAFLNKGINIFFRDEKHAEKEDVNFCYQGG
LSSFVSYLNENKEPLFSNPIYFHGVKPGDDAPIEFEVAMQWNDGYSETIFSYVNNIPTRQGGSHLTGFSTALTRVLNNYI
KNHNLLKSDKISINGEDMREGLTAVISVKVANPQFEGQTKQRLGNSDVGSVVQQIVGEELAIFLDENPSMAKLITDKAII
AAQAREAARKARELTLRKSALDSARLPGKLTDCQEKNPALCEIYIVEGDSAGGSAKSGRDRRFQAILPIRGKILNVEKAR
LEKVLQNTEVGTMVAALGCGIGKDGFNLEKLRYHKVIIMTDADVDGSHIRTLLLTFFYRHMPALVENNFIYIAQPPLYRV
SRKKTSRYIHSEREMDDYLLELGLSDIQIKLASRENILSLEETKKLVSTILAVEIFIDRIEKKGIPFREFLALKNENGLL
PRFQMNLMEGARFAYSEDEFVALRQSEEESQRQRHQETLASIPADEITPEMRTFKPSRLHFIELYEEGGIEEIQEPLQEY
GLDFSYYMCANGVILDIVEEGGKTYTYYTLREVIDFLRNNGRKGIEIQRYKGLGEMNADQLWETTMDPVKRTLIKVTLPD
VIAADHMFTMLMGEDVPPRRAFIEQHALSVKNLDV

Sequences:

>Translated_835_residues
MSQDTLSSDKPKIQLKEYDASSITVLEGLQAVRERPGMYIGDTGVNGLHHLVYEVIDNCIDEAMAGYCTAIDVVLHKDNS
ISIEDDGRGIPVERHENESRKQGREVSAIEVVMTILHAGGKFDKDTYKVSGGLHGVGVSCVNALSKKMIVQVCKNGLMHE
IEFSQGKVVKPVQVIGETTKRGTKVWFWPDPEVMTTIDFDYDILAKRFRELAFLNKGINIFFRDEKHAEKEDVNFCYQGG
LSSFVSYLNENKEPLFSNPIYFHGVKPGDDAPIEFEVAMQWNDGYSETIFSYVNNIPTRQGGSHLTGFSTALTRVLNNYI
KNHNLLKSDKISINGEDMREGLTAVISVKVANPQFEGQTKQRLGNSDVGSVVQQIVGEELAIFLDENPSMAKLITDKAII
AAQAREAARKARELTLRKSALDSARLPGKLTDCQEKNPALCEIYIVEGDSAGGSAKSGRDRRFQAILPIRGKILNVEKAR
LEKVLQNTEVGTMVAALGCGIGKDGFNLEKLRYHKVIIMTDADVDGSHIRTLLLTFFYRHMPALVENNFIYIAQPPLYRV
SRKKTSRYIHSEREMDDYLLELGLSDIQIKLASRENILSLEETKKLVSTILAVEIFIDRIEKKGIPFREFLALKNENGLL
PRFQMNLMEGARFAYSEDEFVALRQSEEESQRQRHQETLASIPADEITPEMRTFKPSRLHFIELYEEGGIEEIQEPLQEY
GLDFSYYMCANGVILDIVEEGGKTYTYYTLREVIDFLRNNGRKGIEIQRYKGLGEMNADQLWETTMDPVKRTLIKVTLPD
VIAADHMFTMLMGEDVPPRRAFIEQHALSVKNLDV
>Mature_834_residues
SQDTLSSDKPKIQLKEYDASSITVLEGLQAVRERPGMYIGDTGVNGLHHLVYEVIDNCIDEAMAGYCTAIDVVLHKDNSI
SIEDDGRGIPVERHENESRKQGREVSAIEVVMTILHAGGKFDKDTYKVSGGLHGVGVSCVNALSKKMIVQVCKNGLMHEI
EFSQGKVVKPVQVIGETTKRGTKVWFWPDPEVMTTIDFDYDILAKRFRELAFLNKGINIFFRDEKHAEKEDVNFCYQGGL
SSFVSYLNENKEPLFSNPIYFHGVKPGDDAPIEFEVAMQWNDGYSETIFSYVNNIPTRQGGSHLTGFSTALTRVLNNYIK
NHNLLKSDKISINGEDMREGLTAVISVKVANPQFEGQTKQRLGNSDVGSVVQQIVGEELAIFLDENPSMAKLITDKAIIA
AQAREAARKARELTLRKSALDSARLPGKLTDCQEKNPALCEIYIVEGDSAGGSAKSGRDRRFQAILPIRGKILNVEKARL
EKVLQNTEVGTMVAALGCGIGKDGFNLEKLRYHKVIIMTDADVDGSHIRTLLLTFFYRHMPALVENNFIYIAQPPLYRVS
RKKTSRYIHSEREMDDYLLELGLSDIQIKLASRENILSLEETKKLVSTILAVEIFIDRIEKKGIPFREFLALKNENGLLP
RFQMNLMEGARFAYSEDEFVALRQSEEESQRQRHQETLASIPADEITPEMRTFKPSRLHFIELYEEGGIEEIQEPLQEYG
LDFSYYMCANGVILDIVEEGGKTYTYYTLREVIDFLRNNGRKGIEIQRYKGLGEMNADQLWETTMDPVKRTLIKVTLPDV
IAADHMFTMLMGEDVPPRRAFIEQHALSVKNLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=606, Percent_Identity=25.9075907590759, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI19913408, Length=599, Percent_Identity=26.5442404006678, Blast_Score=143, Evalue=6e-34,
Organism=Escherichia coli, GI48994957, Length=835, Percent_Identity=48.8622754491018, Blast_Score=742, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=611, Percent_Identity=39.2798690671031, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17535065, Length=565, Percent_Identity=26.0176991150442, Blast_Score=149, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI212645845, Length=528, Percent_Identity=25.1893939393939, Blast_Score=138, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI212645657, Length=191, Percent_Identity=30.3664921465969, Blast_Score=79, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=615, Percent_Identity=25.5284552845528, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI17136538, Length=559, Percent_Identity=25.7602862254025, Blast_Score=143, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 94247; Mature: 94116

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDTLSSDKPKIQLKEYDASSITVLEGLQAVRERPGMYIGDTGVNGLHHLVYEVIDNCI
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHH
DEAMAGYCTAIDVVLHKDNSISIEDDGRGIPVERHENESRKQGREVSAIEVVMTILHAGG
HHHHHHHHHEEEEEEECCCEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCC
KFDKDTYKVSGGLHGVGVSCVNALSKKMIVQVCKNGLMHEIEFSQGKVVKPVQVIGETTK
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCC
RGTKVWFWPDPEVMTTIDFDYDILAKRFRELAFLNKGINIFFRDEKHAEKEDVNFCYQGG
CCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCC
LSSFVSYLNENKEPLFSNPIYFHGVKPGDDAPIEFEVAMQWNDGYSETIFSYVNNIPTRQ
HHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCC
GGSHLTGFSTALTRVLNNYIKNHNLLKSDKISINGEDMREGLTAVISVKVANPQFEGQTK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHCCCEEEEEEEECCCCCCCCHH
QRLGNSDVGSVVQQIVGEELAIFLDENPSMAKLITDKAIIAAQAREAARKARELTLRKSA
HHCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSARLPGKLTDCQEKNPALCEIYIVEGDSAGGSAKSGRDRRFQAILPIRGKILNVEKAR
HHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEECHHHHH
LEKVLQNTEVGTMVAALGCGIGKDGFNLEKLRYHKVIIMTDADVDGSHIRTLLLTFFYRH
HHHHHHCCCHHHHHHHHHCCCCCCCCCHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHH
MPALVENNFIYIAQPPLYRVSRKKTSRYIHSEREMDDYLLELGLSDIQIKLASRENILSL
HHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCH
EETKKLVSTILAVEIFIDRIEKKGIPFREFLALKNENGLLPRFQMNLMEGARFAYSEDEF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCE
VALRQSEEESQRQRHQETLASIPADEITPEMRTFKPSRLHFIELYEEGGIEEIQEPLQEY
EEECCCHHHHHHHHHHHHHHHCCHHHCCCHHHCCCCCCEEEEEEECCCCHHHHHHHHHHH
GLDFSYYMCANGVILDIVEEGGKTYTYYTLREVIDFLRNNGRKGIEIQRYKGLGEMNADQ
CCCEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHH
LWETTMDPVKRTLIKVTLPDVIAADHMFTMLMGEDVPPRRAFIEQHALSVKNLDV
HHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure 
SQDTLSSDKPKIQLKEYDASSITVLEGLQAVRERPGMYIGDTGVNGLHHLVYEVIDNCI
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHH
DEAMAGYCTAIDVVLHKDNSISIEDDGRGIPVERHENESRKQGREVSAIEVVMTILHAGG
HHHHHHHHHEEEEEEECCCEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCC
KFDKDTYKVSGGLHGVGVSCVNALSKKMIVQVCKNGLMHEIEFSQGKVVKPVQVIGETTK
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCC
RGTKVWFWPDPEVMTTIDFDYDILAKRFRELAFLNKGINIFFRDEKHAEKEDVNFCYQGG
CCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCC
LSSFVSYLNENKEPLFSNPIYFHGVKPGDDAPIEFEVAMQWNDGYSETIFSYVNNIPTRQ
HHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCC
GGSHLTGFSTALTRVLNNYIKNHNLLKSDKISINGEDMREGLTAVISVKVANPQFEGQTK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHCCCEEEEEEEECCCCCCCCHH
QRLGNSDVGSVVQQIVGEELAIFLDENPSMAKLITDKAIIAAQAREAARKARELTLRKSA
HHCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSARLPGKLTDCQEKNPALCEIYIVEGDSAGGSAKSGRDRRFQAILPIRGKILNVEKAR
HHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEECHHHHH
LEKVLQNTEVGTMVAALGCGIGKDGFNLEKLRYHKVIIMTDADVDGSHIRTLLLTFFYRH
HHHHHHCCCHHHHHHHHHCCCCCCCCCHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHH
MPALVENNFIYIAQPPLYRVSRKKTSRYIHSEREMDDYLLELGLSDIQIKLASRENILSL
HHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCH
EETKKLVSTILAVEIFIDRIEKKGIPFREFLALKNENGLLPRFQMNLMEGARFAYSEDEF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCE
VALRQSEEESQRQRHQETLASIPADEITPEMRTFKPSRLHFIELYEEGGIEEIQEPLQEY
EEECCCHHHHHHHHHHHHHHHCCHHHCCCHHHCCCCCCEEEEEEECCCCHHHHHHHHHHH
GLDFSYYMCANGVILDIVEEGGKTYTYYTLREVIDFLRNNGRKGIEIQRYKGLGEMNADQ
CCCEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHH
LWETTMDPVKRTLIKVTLPDVIAADHMFTMLMGEDVPPRRAFIEQHALSVKNLDV
HHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]