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The map label for this gene is folE [H]

Identifier: 46446083

GI number: 46446083

Start: 577540

End: 578214

Strand: Reverse

Name: folE [H]

Synonym: pc0449

Alternate gene names: 46446083

Gene position: 578214-577540 (Counterclockwise)

Preceding gene: 46446084

Following gene: 46446082

Centisome position: 23.95

GC content: 36.15

Gene sequence:

>675_bases
ATGACAAAAAAAGAGTTCCCTCAAAAAAAAATTCCTCCTTCTATCACAAATTTTCCTTCACCGATTCGGCGACATCAAAA
GCGCTTGTCGGATCAAGATAAAATAAAGCTAATTGCTGAACATTTTAAAGCCATCATGGAGACACTTGAGCTTGATGTGG
AAGATGAATCTTTAGCTCGTACCCCAGAACGAGTAGCCAAAATGTACGTTAATGAAATTTTTTCGGGATTAGATGAAGAA
AATTTTCCTGAAATAAGTTTTTTCAAAGATGAAATTCATCACGAACATAAAGCCAATATGGTTTTTGTTAAAGTCGGCTT
TACAAGTTTTTGTGAGCATCACTTTGTTCCCATGTCAGGTTTTGCTTATGTTGCCTATATCCCTACAAATCAATTAATTG
GGTTATCTAAAATTCCTCGGCTGGTGCGATATTTTGCTAAAAGGCCTCAATTACAAGAAAGATTAACTGCTCAAATTGCT
GACTCTTTAGCCCTTCTTTTAAATACTGAGCATGTCGCCGTCTCTTTGACAGCGATTCATTCTTGTGTTCAGGCTAGAGG
AATTCAAGATGAATGTAGCCATACGATTACAAATGTTTTACGAGGTCAATTCGGAGCTGATGGAAATTTAAGACGTGAGT
TTTTTGAAGGAATCAATCGAAGAGTTTTTGAATAA

Upstream 100 bases:

>100_bases
AATGCTTTCGCTATTACGGCAGGTGAAATCTATCGTCAAAGGAAAACTGCGAAAATTTATGAATACCTTGCCTTAGAACC
TAATAATGAGTAAAAAATTC

Downstream 100 bases:

>100_bases
AAAAAATTATTGGATTAATATGAATTAAAATTAAAAGAAATTCTTATTAAATAAATAACAAATCCAGATAGAGGAAAAAA
TGTCTACATCAATATTGTCC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTKKEFPQKKIPPSITNFPSPIRRHQKRLSDQDKIKLIAEHFKAIMETLELDVEDESLARTPERVAKMYVNEIFSGLDEE
NFPEISFFKDEIHHEHKANMVFVKVGFTSFCEHHFVPMSGFAYVAYIPTNQLIGLSKIPRLVRYFAKRPQLQERLTAQIA
DSLALLLNTEHVAVSLTAIHSCVQARGIQDECSHTITNVLRGQFGADGNLRREFFEGINRRVFE

Sequences:

>Translated_224_residues
MTKKEFPQKKIPPSITNFPSPIRRHQKRLSDQDKIKLIAEHFKAIMETLELDVEDESLARTPERVAKMYVNEIFSGLDEE
NFPEISFFKDEIHHEHKANMVFVKVGFTSFCEHHFVPMSGFAYVAYIPTNQLIGLSKIPRLVRYFAKRPQLQERLTAQIA
DSLALLLNTEHVAVSLTAIHSCVQARGIQDECSHTITNVLRGQFGADGNLRREFFEGINRRVFE
>Mature_223_residues
TKKEFPQKKIPPSITNFPSPIRRHQKRLSDQDKIKLIAEHFKAIMETLELDVEDESLARTPERVAKMYVNEIFSGLDEEN
FPEISFFKDEIHHEHKANMVFVKVGFTSFCEHHFVPMSGFAYVAYIPTNQLIGLSKIPRLVRYFAKRPQLQERLTAQIAD
SLALLLNTEHVAVSLTAIHSCVQARGIQDECSHTITNVLRGQFGADGNLRREFFEGINRRVFE

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=179, Percent_Identity=34.6368715083799, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI4503949, Length=179, Percent_Identity=34.6368715083799, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI66932970, Length=152, Percent_Identity=32.8947368421053, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI66932972, Length=145, Percent_Identity=33.1034482758621, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1788476, Length=200, Percent_Identity=49, Blast_Score=185, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17560486, Length=203, Percent_Identity=34.9753694581281, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321706, Length=189, Percent_Identity=38.6243386243386, Blast_Score=118, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24656791, Length=187, Percent_Identity=35.2941176470588, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24656796, Length=187, Percent_Identity=35.2941176470588, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24656782, Length=187, Percent_Identity=35.2941176470588, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 25814; Mature: 25683

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKEFPQKKIPPSITNFPSPIRRHQKRLSDQDKIKLIAEHFKAIMETLELDVEDESLAR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
TPERVAKMYVNEIFSGLDEENFPEISFFKDEIHHEHKANMVFVKVGFTSFCEHHFVPMSG
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHCCCCCCC
FAYVAYIPTNQLIGLSKIPRLVRYFAKRPQLQERLTAQIADSLALLLNTEHVAVSLTAIH
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SCVQARGIQDECSHTITNVLRGQFGADGNLRREFFEGINRRVFE
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKKEFPQKKIPPSITNFPSPIRRHQKRLSDQDKIKLIAEHFKAIMETLELDVEDESLAR
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
TPERVAKMYVNEIFSGLDEENFPEISFFKDEIHHEHKANMVFVKVGFTSFCEHHFVPMSG
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHCCCCCCC
FAYVAYIPTNQLIGLSKIPRLVRYFAKRPQLQERLTAQIADSLALLLNTEHVAVSLTAIH
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SCVQARGIQDECSHTITNVLRGQFGADGNLRREFFEGINRRVFE
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA