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The map label for this gene is trmH [H]

Identifier: 46446084

GI number: 46446084

Start: 578222

End: 578962

Strand: Reverse

Name: trmH [H]

Synonym: pc0450

Alternate gene names: 46446084

Gene position: 578962-578222 (Counterclockwise)

Preceding gene: 46446085

Following gene: 46446083

Centisome position: 23.98

GC content: 33.87

Gene sequence:

>741_bases
TTGGAAAGACAACATAAAAAATGCGCAGAGCTAGTTCGTTACTTATATGAACTTTCTCTGCAAAATCAACTAAATCCTTC
ACTCCTTATGCTTTATCACGAATTTCTTAATTGGATGAAAGAAAATCCTCTTAATAATTCTACTATTCAAGAACTTTCTA
ACCGTTACCATTACCACCTAAAAAAAGCAAAACTTCAAAAGAAAGAACATCATCTTTTATCAACTGTACGCACGCAAGAT
CGTTCTACAGGAAACCCTAGCTGGCCAATTTCTATCTATCTGGACAATTTACGGTCAGCTCATAACGTGGGAAGTATTAT
CAGAACAGTAGAAGCTTTCTCCTTAGGCAGCATTTATTTTTCTACCCAAACTCCCTATACAAATCATAAACAAGTACAAA
ATGTTTCTATGGGAGCTTATCAATGGGTAAAATGTTATCAAAATGAAGATTTAAATGATTTGACACAACCTATTATTGCT
TTAGAAACTTCTGAGGAAGCTATTAATCTATACGAATTTATCTTTCCAGAGCATTTCACATTAGTAGTGGGGAACGAAGA
ATATGGTTGTAGCCAAGAAATTCTTACAAAAGCACATTATTTAATAGAAATCCCTTTAAGAGGCAGGAAAAACTCTTTAA
ATGTTGCCAATGCTTTCGCTATTACGGCAGGTGAAATCTATCGTCAAAGGAAAACTGCGAAAATTTATGAATACCTTGCC
TTAGAACCTAATAATGAGTAA

Upstream 100 bases:

>100_bases
TTTCTTTGAAGGCAATCTGGAAGAGTATTTATTACATAAAAGTCATTCTTAATCTCTAATTATGTACAATTTTACTTCCA
GGAAGTTTCTAAATCTCCCC

Downstream 100 bases:

>100_bases
AAAATTCATGACAAAAAAAGAGTTCCCTCAAAAAAAAATTCCTCCTTCTATCACAAATTTTCCTTCACCGATTCGGCGAC
ATCAAAAGCGCTTGTCGGAT

Product: putative tRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MERQHKKCAELVRYLYELSLQNQLNPSLLMLYHEFLNWMKENPLNNSTIQELSNRYHYHLKKAKLQKKEHHLLSTVRTQD
RSTGNPSWPISIYLDNLRSAHNVGSIIRTVEAFSLGSIYFSTQTPYTNHKQVQNVSMGAYQWVKCYQNEDLNDLTQPIIA
LETSEEAINLYEFIFPEHFTLVVGNEEYGCSQEILTKAHYLIEIPLRGRKNSLNVANAFAITAGEIYRQRKTAKIYEYLA
LEPNNE

Sequences:

>Translated_246_residues
MERQHKKCAELVRYLYELSLQNQLNPSLLMLYHEFLNWMKENPLNNSTIQELSNRYHYHLKKAKLQKKEHHLLSTVRTQD
RSTGNPSWPISIYLDNLRSAHNVGSIIRTVEAFSLGSIYFSTQTPYTNHKQVQNVSMGAYQWVKCYQNEDLNDLTQPIIA
LETSEEAINLYEFIFPEHFTLVVGNEEYGCSQEILTKAHYLIEIPLRGRKNSLNVANAFAITAGEIYRQRKTAKIYEYLA
LEPNNE
>Mature_246_residues
MERQHKKCAELVRYLYELSLQNQLNPSLLMLYHEFLNWMKENPLNNSTIQELSNRYHYHLKKAKLQKKEHHLLSTVRTQD
RSTGNPSWPISIYLDNLRSAHNVGSIIRTVEAFSLGSIYFSTQTPYTNHKQVQNVSMGAYQWVKCYQNEDLNDLTQPIIA
LETSEEAINLYEFIFPEHFTLVVGNEEYGCSQEILTKAHYLIEIPLRGRKNSLNVANAFAITAGEIYRQRKTAKIYEYLA
LEPNNE

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=154, Percent_Identity=26.6233766233766, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 28732; Mature: 28732

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERQHKKCAELVRYLYELSLQNQLNPSLLMLYHEFLNWMKENPLNNSTIQELSNRYHYHL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KKAKLQKKEHHLLSTVRTQDRSTGNPSWPISIYLDNLRSAHNVGSIIRTVEAFSLGSIYF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEE
STQTPYTNHKQVQNVSMGAYQWVKCYQNEDLNDLTQPIIALETSEEAINLYEFIFPEHFT
ECCCCCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHEEEECCHHHHHHHHHHCCCCEE
LVVGNEEYGCSQEILTKAHYLIEIPLRGRKNSLNVANAFAITAGEIYRQRKTAKIYEYLA
EEECCCCCCCHHHHHHHCEEEEEECCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHC
LEPNNE
CCCCCC
>Mature Secondary Structure
MERQHKKCAELVRYLYELSLQNQLNPSLLMLYHEFLNWMKENPLNNSTIQELSNRYHYHL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KKAKLQKKEHHLLSTVRTQDRSTGNPSWPISIYLDNLRSAHNVGSIIRTVEAFSLGSIYF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEE
STQTPYTNHKQVQNVSMGAYQWVKCYQNEDLNDLTQPIIALETSEEAINLYEFIFPEHFT
ECCCCCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHEEEECCHHHHHHHHHHCCCCEE
LVVGNEEYGCSQEILTKAHYLIEIPLRGRKNSLNVANAFAITAGEIYRQRKTAKIYEYLA
EEECCCCCCCHHHHHHHCEEEEEECCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHC
LEPNNE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]