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The map label for this gene is nfo

Identifier: 46445979

GI number: 46445979

Start: 469268

End: 470116

Strand: Direct

Name: nfo

Synonym: pc0345

Alternate gene names: 46445979

Gene position: 469268-470116 (Clockwise)

Preceding gene: 46445975

Following gene: 46445980

Centisome position: 19.44

GC content: 35.57

Gene sequence:

>849_bases
ATGAGTCATTCGAATTTACTGCTTGGCGCACATACTTCTGCAGCAGGCGGGGTTTACCGTGCTTTATTAGAAGGAAAAAA
AATTGGGGCAACAACTATTCAATTTTTTACAAGTAATCAAAAACAGTGGAAAGGTCGGCAATTTACAACGAATGATATTG
AATTATGGCAAAGTACTCTTAAAGAGACAAATCTTACTCATCTGATGAGTCATGATAGTTATTTGATCAATTTAGGGTGC
CCTAATCAAGAAAATCTTTTGAAAAGTAGGCAAGCTTTTCAAGAAGAAGTTATTAGATGTACACAATTAGGTATAAATTA
TTTAAATTTTCATCCAGGTGCTTCACTTGGAGAAGATGTGCAGAAGTGCTTAGATTCTATTGTGGAAAGTTTATTGTTAG
TACGCCCTTTTATTCAAGGCAATTTACGTTTACTTCTTGAAGCTACTGCGGGACAAGGTACTTCTGTCGGACATAAGTTT
GAACAGCTTGCTTATATCATTAATGGAGTGAAAGATGAACTTCCAATTGGAGTTTGCATTGATACCTGTCATATTTTTGT
AGCAGGATATGATATACGCACGTCTTCAGCTTGGGATTTTACATTAAAGGGATTTGATCGAATCATTGGTTTGCCTTACC
TTTATGCATTTCATATTAATGATTCTTCTAAAGATTTAGGTTCAAGAGTTGATCGTCACCAACCTTTGGGTGAAGGAAAA
ATTGGGTGGGAAAGCTTCGAATTTTTAATGAAAGATTCTCGAACAAGGCATTTGCCCAAATATCTCGAAACCCCAGGGGG
TGTAGATCTATGGGAAAAAGAAATTCAAAAATTGAAAGAATTTGCTTAA

Upstream 100 bases:

>100_bases
TTACCTTGTTAAGATCTCAGCTTTATGTATTACAGGTTCAAAAACAGCAAGCTCCTTTGATAGGAGATTAGCCTTCAATG
TTACCATTATAAGGAATCTT

Downstream 100 bases:

>100_bases
TAAACTTTTGTGCTTTTTTCTTTTTTACCAAATTCAATTGGTAAATATTTTTAAAACTTCTTCAAATTTGAAGAAAAAAA
CCAGCAAAAAAATTCAAATT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSHSNLLLGAHTSAAGGVYRALLEGKKIGATTIQFFTSNQKQWKGRQFTTNDIELWQSTLKETNLTHLMSHDSYLINLGC
PNQENLLKSRQAFQEEVIRCTQLGINYLNFHPGASLGEDVQKCLDSIVESLLLVRPFIQGNLRLLLEATAGQGTSVGHKF
EQLAYIINGVKDELPIGVCIDTCHIFVAGYDIRTSSAWDFTLKGFDRIIGLPYLYAFHINDSSKDLGSRVDRHQPLGEGK
IGWESFEFLMKDSRTRHLPKYLETPGGVDLWEKEIQKLKEFA

Sequences:

>Translated_282_residues
MSHSNLLLGAHTSAAGGVYRALLEGKKIGATTIQFFTSNQKQWKGRQFTTNDIELWQSTLKETNLTHLMSHDSYLINLGC
PNQENLLKSRQAFQEEVIRCTQLGINYLNFHPGASLGEDVQKCLDSIVESLLLVRPFIQGNLRLLLEATAGQGTSVGHKF
EQLAYIINGVKDELPIGVCIDTCHIFVAGYDIRTSSAWDFTLKGFDRIIGLPYLYAFHINDSSKDLGSRVDRHQPLGEGK
IGWESFEFLMKDSRTRHLPKYLETPGGVDLWEKEIQKLKEFA
>Mature_281_residues
SHSNLLLGAHTSAAGGVYRALLEGKKIGATTIQFFTSNQKQWKGRQFTTNDIELWQSTLKETNLTHLMSHDSYLINLGCP
NQENLLKSRQAFQEEVIRCTQLGINYLNFHPGASLGEDVQKCLDSIVESLLLVRPFIQGNLRLLLEATAGQGTSVGHKFE
QLAYIINGVKDELPIGVCIDTCHIFVAGYDIRTSSAWDFTLKGFDRIIGLPYLYAFHINDSSKDLGSRVDRHQPLGEGKI
GWESFEFLMKDSRTRHLPKYLETPGGVDLWEKEIQKLKEFA

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=275, Percent_Identity=46.1818181818182, Blast_Score=245, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17531193, Length=267, Percent_Identity=43.8202247191011, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6322735, Length=268, Percent_Identity=35.4477611940299, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_PARUW (Q6MED0)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007344.1
- ProteinModelPortal:   Q6MED0
- SMR:   Q6MED0
- STRING:   Q6MED0
- GeneID:   2780455
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0345
- NMPDR:   fig|264201.1.peg.345
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- PhylomeDB:   Q6MED0
- ProtClustDB:   PRK01060
- BioCyc:   CPRO264201:PC0345-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31762; Mature: 31630

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSNLLLGAHTSAAGGVYRALLEGKKIGATTIQFFTSNQKQWKGRQFTTNDIELWQSTL
CCCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCCCCHHHHHHHHHH
KETNLTHLMSHDSYLINLGCPNQENLLKSRQAFQEEVIRCTQLGINYLNFHPGASLGEDV
HHCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHH
QKCLDSIVESLLLVRPFIQGNLRLLLEATAGQGTSVGHKFEQLAYIINGVKDELPIGVCI
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCHHHCCCHHHEE
DTCHIFVAGYDIRTSSAWDFTLKGFDRIIGLPYLYAFHINDSSKDLGSRVDRHQPLGEGK
HHEEEEEEECEEECCCCCEEHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCC
IGWESFEFLMKDSRTRHLPKYLETPGGVDLWEKEIQKLKEFA
CCHHHHHHHHHCCCHHCCHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHSNLLLGAHTSAAGGVYRALLEGKKIGATTIQFFTSNQKQWKGRQFTTNDIELWQSTL
CCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCCCCHHHHHHHHHH
KETNLTHLMSHDSYLINLGCPNQENLLKSRQAFQEEVIRCTQLGINYLNFHPGASLGEDV
HHCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHH
QKCLDSIVESLLLVRPFIQGNLRLLLEATAGQGTSVGHKFEQLAYIINGVKDELPIGVCI
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCHHHCCCHHHEE
DTCHIFVAGYDIRTSSAWDFTLKGFDRIIGLPYLYAFHINDSSKDLGSRVDRHQPLGEGK
HHEEEEEEECEEECCCCCEEHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCC
IGWESFEFLMKDSRTRHLPKYLETPGGVDLWEKEIQKLKEFA
CCHHHHHHHHHCCCHHCCHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA