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The map label for this gene is amiA [H]

Identifier: 46445949

GI number: 46445949

Start: 433249

End: 434091

Strand: Direct

Name: amiA [H]

Synonym: pc0315

Alternate gene names: 46445949

Gene position: 433249-434091 (Clockwise)

Preceding gene: 46445948

Following gene: 46445950

Centisome position: 17.94

GC content: 34.76

Gene sequence:

>843_bases
ATGTTTCGTATTATACAATGGTTGAATTTCAGTCTAATATTAATTGGCCATACACTATACGCTCAACCACCTTATAAAGC
TAATTTTCCTTTGCCTAAGACTAATCCTATTAAGCAACTTTATTCTAAAGAAGTGTTAGAATGCATTAGTAAGCCACTTC
TCATAAAAAAAGCTTTTATTGTGATTGATCCTGGTCATGGAGGGCATGATGTGGGGACTCAATCTATTTCTAAACCTCGG
TATCAAGAAAAATCGTTGAATTTAGTCACTGCGAAATTTGTTTGTAGTTATTTGCAGCAACTTGGATATCAGATATTTAT
GACGCGCGAGAACGATAAATTTATTTCATTAGATAAACGAGCGCAAATAGCTAATAAACGGAAACCTACTTTGTTTGTCA
GCGTCCATTATAACTCAGCTCCTAGTAGCGAAGCTCAAGGGGTAGAAGTCTTTTTTTATCAATCTGATGATAAAGAACGC
GCACGTAAATCTAAACGGTTAGCTCAACTCATTTTACAAACTGTTTTAACAGAAACAGAAGCTAAATCTCGTGGAGTTAA
GCATGGAAATTTCGCTGTCATTCGTGAGACGAATATGCCTGCAGTGCTAATTGAAGGAGGCTTTGTTACAAATGAAGAAG
AATTAAAAAAATTAAAAGATCCGGCCTATCTAAAAAAAATTGCTTTGGGCATCGCCAAGGGAATTGACGAATATGTCAGA
AGAATAGGCAAAACTGAAAATAAAAAAGAGATCAGTGCACCAAAAAAATTTAAAAAACTAGCTAAGCGTTTAACTTTAGA
GACCCCTATCTCAAATTTAAGTCATACAATTGATCTCTTTTGA

Upstream 100 bases:

>100_bases
TTATCTCATCAAATTATTGAGTTTGATGATGCAAAAGTTGCTGCTGAGATATGTCTGCAGCAAAATAAATGATTTACTTA
ATTTTTTTTAAGAGACATGC

Downstream 100 bases:

>100_bases
CCGGATTAAAATTGCTTTTTTAGTTGAGTAATTATTCATTGCAAGTGAATCATTGTTATTTTTAATTCTCTAAATTTTAA
TCGATTAATAATTAGATTCT

Product: putative N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MFRIIQWLNFSLILIGHTLYAQPPYKANFPLPKTNPIKQLYSKEVLECISKPLLIKKAFIVIDPGHGGHDVGTQSISKPR
YQEKSLNLVTAKFVCSYLQQLGYQIFMTRENDKFISLDKRAQIANKRKPTLFVSVHYNSAPSSEAQGVEVFFYQSDDKER
ARKSKRLAQLILQTVLTETEAKSRGVKHGNFAVIRETNMPAVLIEGGFVTNEEELKKLKDPAYLKKIALGIAKGIDEYVR
RIGKTENKKEISAPKKFKKLAKRLTLETPISNLSHTIDLF

Sequences:

>Translated_280_residues
MFRIIQWLNFSLILIGHTLYAQPPYKANFPLPKTNPIKQLYSKEVLECISKPLLIKKAFIVIDPGHGGHDVGTQSISKPR
YQEKSLNLVTAKFVCSYLQQLGYQIFMTRENDKFISLDKRAQIANKRKPTLFVSVHYNSAPSSEAQGVEVFFYQSDDKER
ARKSKRLAQLILQTVLTETEAKSRGVKHGNFAVIRETNMPAVLIEGGFVTNEEELKKLKDPAYLKKIALGIAKGIDEYVR
RIGKTENKKEISAPKKFKKLAKRLTLETPISNLSHTIDLF
>Mature_280_residues
MFRIIQWLNFSLILIGHTLYAQPPYKANFPLPKTNPIKQLYSKEVLECISKPLLIKKAFIVIDPGHGGHDVGTQSISKPR
YQEKSLNLVTAKFVCSYLQQLGYQIFMTRENDKFISLDKRAQIANKRKPTLFVSVHYNSAPSSEAQGVEVFFYQSDDKER
ARKSKRLAQLILQTVLTETEAKSRGVKHGNFAVIRETNMPAVLIEGGFVTNEEELKKLKDPAYLKKIALGIAKGIDEYVR
RIGKTENKKEISAPKKFKKLAKRLTLETPISNLSHTIDLF

Specific function: Probably involved in cell-wall metabolism [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=222, Percent_Identity=31.5315315315315, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082163, Length=220, Percent_Identity=28.6363636363636, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR017293
- InterPro:   IPR003646
- InterPro:   IPR013247 [H]

Pfam domain/function: PF01520 Amidase_3; PF08239 SH3_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31868; Mature: 31868

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRIIQWLNFSLILIGHTLYAQPPYKANFPLPKTNPIKQLYSKEVLECISKPLLIKKAFI
CCHHHHHHCCEEEEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
VIDPGHGGHDVGTQSISKPRYQEKSLNLVTAKFVCSYLQQLGYQIFMTRENDKFISLDKR
EEECCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECHHH
AQIANKRKPTLFVSVHYNSAPSSEAQGVEVFFYQSDDKERARKSKRLAQLILQTVLTETE
HHHHCCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AKSRGVKHGNFAVIRETNMPAVLIEGGFVTNEEELKKLKDPAYLKKIALGIAKGIDEYVR
HHHCCCCCCCEEEEEECCCCEEEEECCEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RIGKTENKKEISAPKKFKKLAKRLTLETPISNLSHTIDLF
HHCCCCCCHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MFRIIQWLNFSLILIGHTLYAQPPYKANFPLPKTNPIKQLYSKEVLECISKPLLIKKAFI
CCHHHHHHCCEEEEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
VIDPGHGGHDVGTQSISKPRYQEKSLNLVTAKFVCSYLQQLGYQIFMTRENDKFISLDKR
EEECCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECHHH
AQIANKRKPTLFVSVHYNSAPSSEAQGVEVFFYQSDDKERARKSKRLAQLILQTVLTETE
HHHHCCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AKSRGVKHGNFAVIRETNMPAVLIEGGFVTNEEELKKLKDPAYLKKIALGIAKGIDEYVR
HHHCCCCCCCEEEEEECCCCEEEEECCEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RIGKTENKKEISAPKKFKKLAKRLTLETPISNLSHTIDLF
HHCCCCCCHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]