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The map label for this gene is murE [H]

Identifier: 46445948

GI number: 46445948

Start: 431742

End: 433220

Strand: Direct

Name: murE [H]

Synonym: pc0314

Alternate gene names: 46445948

Gene position: 431742-433220 (Clockwise)

Preceding gene: 46445947

Following gene: 46445949

Centisome position: 17.88

GC content: 34.62

Gene sequence:

>1479_bases
ATGAAGTTAAAAGAATTGTTTAGAAACATTGAATCCTGCCAGTTTAAAGGCTCCCAAGAAGTGTTGTTGACTGGATTGAC
AGCTAACTCCAAATCCCTTAGTCCAGGAAATCTATTTATTGCTAAAAAAGGAAAAATGTTTGATGGCAACTCCTATATAA
AAGAAGCTATTCAATCAGGAGCATGTGCGATCTTAACAGATCTTTTTGATCCTTGCCTAAAAAAAGTTGTGCAAATTATT
CATCCCAATCCTAACGAAATCGAATCTATTTTAGCGCGCGATTACTATCAAAATAGCTCTGAAGAGTTATTTATGGTAGG
AATAACAGGGACAAATGGTAAAACAACCATATCTTTTATTATTAAATATCTTTTAGATCTTTTTCACGGTCCTTGTGGTT
TGATTGGAACAATTGAATATATCGTTGGATCTCATCGATACCAGGCAACACGAACAACCCCTGACGTTATTGAAAATCAT
AAAATGCTTTATGAAATGTCTTTGTCAGGTACAAAATCCGCTGTAATGGAAGTGACTTCACATGCCCTTATTCAAAAAAG
AGTAGAATATATTGATTTTGATGTTGCAGTGTTTTCCAATTTAACTCTCGATCATTTGGATTACCATTTGACAATGGAAA
ATTATTGTGCAGCTAAAAATTGTTTATTTGAGGGATTGGGTGAGCAACCGACCAAAAAAAGAAATGAAAAATGGGCAATT
GTGAATATTGACGATCCATGGTGCGAACAAATAGTCAAAGGATGTCAAGCTCATCGCCTGACTTACGGAATAGATAAGCC
GGCTGATTTACAAGCAAGTCATATTAAATTAAGTTCGCAAGGAACATCTATTAATTTGACTTATCAAGGACAAACAATTG
AATGTTTTTGGCCTCTTTTAGGTCGATTTAATATTTATAACTGCTTAGCCGCGATAGGTGTATTGCTGACTCAAAATATT
CCTTTGAAAGAAATAGTAGAAAAAATGGCATGTATTCCCAATGTGAAAGGGCGTTTAGAAAAAGTTTTAAATAATTTAGA
GCTAGATATTTACATTGACTTTGCTCATACCGACGATGCGTTAAGTAATGTTTTAAAAACACTTTCCGAAATTAAAAAGG
GACGTCTTTTAACAATATTTGGTTGTGGAGGCGATAGAGATCCCAGTAAAAGGCCTAAAATGGCAATAGCCTCAGAGCAA
TATTCTGATTTAAGCATCGTTACTTCAGATAATCCTCGCTCAGAGAACCCCATAAGTATTTGCCAAGAAATTGTAACAGG
TTTTGTTAATCCAAATTCTTTTATTGTAGAAATAGACAGAAGAGCGGCTATTCAAAAAGCCATTGAAATCGCCTCACCAG
GTGATATGATTTTGATTGCTGGGAAAGGGCATGAAACTTATCAAATTTTATCTCATCAAATTATTGAGTTTGATGATGCA
AAAGTTGCTGCTGAGATATGTCTGCAGCAAAATAAATGA

Upstream 100 bases:

>100_bases
CAATCCGGAAAAGGCCAGTTGGATGAATGAAACGAGAAGATTGCAAGAAATATATAAGAAATGGAATAACATCTCCGAAA
ATTAGGGCATAGCGTTTAAA

Downstream 100 bases:

>100_bases
TTTACTTAATTTTTTTTAAGAGACATGCATGTTTCGTATTATACAATGGTTGAATTTCAGTCTAATATTAATTGGCCATA
CACTATACGCTCAACCACCT

Product: putative UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MKLKELFRNIESCQFKGSQEVLLTGLTANSKSLSPGNLFIAKKGKMFDGNSYIKEAIQSGACAILTDLFDPCLKKVVQII
HPNPNEIESILARDYYQNSSEELFMVGITGTNGKTTISFIIKYLLDLFHGPCGLIGTIEYIVGSHRYQATRTTPDVIENH
KMLYEMSLSGTKSAVMEVTSHALIQKRVEYIDFDVAVFSNLTLDHLDYHLTMENYCAAKNCLFEGLGEQPTKKRNEKWAI
VNIDDPWCEQIVKGCQAHRLTYGIDKPADLQASHIKLSSQGTSINLTYQGQTIECFWPLLGRFNIYNCLAAIGVLLTQNI
PLKEIVEKMACIPNVKGRLEKVLNNLELDIYIDFAHTDDALSNVLKTLSEIKKGRLLTIFGCGGDRDPSKRPKMAIASEQ
YSDLSIVTSDNPRSENPISICQEIVTGFVNPNSFIVEIDRRAAIQKAIEIASPGDMILIAGKGHETYQILSHQIIEFDDA
KVAAEICLQQNK

Sequences:

>Translated_492_residues
MKLKELFRNIESCQFKGSQEVLLTGLTANSKSLSPGNLFIAKKGKMFDGNSYIKEAIQSGACAILTDLFDPCLKKVVQII
HPNPNEIESILARDYYQNSSEELFMVGITGTNGKTTISFIIKYLLDLFHGPCGLIGTIEYIVGSHRYQATRTTPDVIENH
KMLYEMSLSGTKSAVMEVTSHALIQKRVEYIDFDVAVFSNLTLDHLDYHLTMENYCAAKNCLFEGLGEQPTKKRNEKWAI
VNIDDPWCEQIVKGCQAHRLTYGIDKPADLQASHIKLSSQGTSINLTYQGQTIECFWPLLGRFNIYNCLAAIGVLLTQNI
PLKEIVEKMACIPNVKGRLEKVLNNLELDIYIDFAHTDDALSNVLKTLSEIKKGRLLTIFGCGGDRDPSKRPKMAIASEQ
YSDLSIVTSDNPRSENPISICQEIVTGFVNPNSFIVEIDRRAAIQKAIEIASPGDMILIAGKGHETYQILSHQIIEFDDA
KVAAEICLQQNK
>Mature_492_residues
MKLKELFRNIESCQFKGSQEVLLTGLTANSKSLSPGNLFIAKKGKMFDGNSYIKEAIQSGACAILTDLFDPCLKKVVQII
HPNPNEIESILARDYYQNSSEELFMVGITGTNGKTTISFIIKYLLDLFHGPCGLIGTIEYIVGSHRYQATRTTPDVIENH
KMLYEMSLSGTKSAVMEVTSHALIQKRVEYIDFDVAVFSNLTLDHLDYHLTMENYCAAKNCLFEGLGEQPTKKRNEKWAI
VNIDDPWCEQIVKGCQAHRLTYGIDKPADLQASHIKLSSQGTSINLTYQGQTIECFWPLLGRFNIYNCLAAIGVLLTQNI
PLKEIVEKMACIPNVKGRLEKVLNNLELDIYIDFAHTDDALSNVLKTLSEIKKGRLLTIFGCGGDRDPSKRPKMAIASEQ
YSDLSIVTSDNPRSENPISICQEIVTGFVNPNSFIVEIDRRAAIQKAIEIASPGDMILIAGKGHETYQILSHQIIEFDDA
KVAAEICLQQNK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=478, Percent_Identity=32.4267782426778, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54898; Mature: 54898

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKELFRNIESCQFKGSQEVLLTGLTANSKSLSPGNLFIAKKGKMFDGNSYIKEAIQSG
CCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCEECCHHHHHHHHHCC
ACAILTDLFDPCLKKVVQIIHPNPNEIESILARDYYQNSSEELFMVGITGTNGKTTISFI
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
IKYLLDLFHGPCGLIGTIEYIVGSHRYQATRTTPDVIENHKMLYEMSLSGTKSAVMEVTS
HHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCHHHHHCCCEEEEEECCCCHHHHHHHHH
HALIQKRVEYIDFDVAVFSNLTLDHLDYHLTMENYCAAKNCLFEGLGEQPTKKRNEKWAI
HHHHHHHHHHHCCHHHHHCCCCHHHCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
VNIDDPWCEQIVKGCQAHRLTYGIDKPADLQASHIKLSSQGTSINLTYQGQTIECFWPLL
EECCCHHHHHHHHHHHHCCEEECCCCCCCCCHHEEEEECCCCEEEEEECCCEEEEHHHHH
GRFNIYNCLAAIGVLLTQNIPLKEIVEKMACIPNVKGRLEKVLNNLELDIYIDFAHTDDA
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEEECCCCHH
LSNVLKTLSEIKKGRLLTIFGCGGDRDPSKRPKMAIASEQYSDLSIVTSDNPRSENPISI
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCHHHH
CQEIVTGFVNPNSFIVEIDRRAAIQKAIEIASPGDMILIAGKGHETYQILSHQIIEFDDA
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCH
KVAAEICLQQNK
HHHHHHHHCCCC
>Mature Secondary Structure
MKLKELFRNIESCQFKGSQEVLLTGLTANSKSLSPGNLFIAKKGKMFDGNSYIKEAIQSG
CCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCEECCHHHHHHHHHCC
ACAILTDLFDPCLKKVVQIIHPNPNEIESILARDYYQNSSEELFMVGITGTNGKTTISFI
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
IKYLLDLFHGPCGLIGTIEYIVGSHRYQATRTTPDVIENHKMLYEMSLSGTKSAVMEVTS
HHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCHHHHHCCCEEEEEECCCCHHHHHHHHH
HALIQKRVEYIDFDVAVFSNLTLDHLDYHLTMENYCAAKNCLFEGLGEQPTKKRNEKWAI
HHHHHHHHHHHCCHHHHHCCCCHHHCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
VNIDDPWCEQIVKGCQAHRLTYGIDKPADLQASHIKLSSQGTSINLTYQGQTIECFWPLL
EECCCHHHHHHHHHHHHCCEEECCCCCCCCCHHEEEEECCCCEEEEEECCCEEEEHHHHH
GRFNIYNCLAAIGVLLTQNIPLKEIVEKMACIPNVKGRLEKVLNNLELDIYIDFAHTDDA
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEEECCCCHH
LSNVLKTLSEIKKGRLLTIFGCGGDRDPSKRPKMAIASEQYSDLSIVTSDNPRSENPISI
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCHHHH
CQEIVTGFVNPNSFIVEIDRRAAIQKAIEIASPGDMILIAGKGHETYQILSHQIIEFDDA
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCH
KVAAEICLQQNK
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]