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The map label for this gene is clpB [H]

Identifier: 46445866

GI number: 46445866

Start: 329603

End: 330727

Strand: Direct

Name: clpB [H]

Synonym: pc0232

Alternate gene names: 46445866

Gene position: 329603-330727 (Clockwise)

Preceding gene: 46445864

Following gene: 46445867

Centisome position: 13.65

GC content: 33.96

Gene sequence:

>1125_bases
ATGAAAAAGATTCTTTTAGTAATTAGCTTTTTAGCTGTCTCTTTAATTAAAGCAGAAAATTTATCCATAACCACTGAAGA
GAAAGAACTAACTTTATCTTTACAAGAAATTGATCATTTACAAGCTTGGTGGCCTTGGTCAAGTACACCTAAAGTCGTCA
ATCCTAAACCTTTATCTCCTTTTAACGTATTAGAACTAGAAAATAATTTAAGAGAAAAAGTGATTGGGCAGTCGCAAGCT
GTTAAAAATACGGCTAATGCATTATTGTGTTATTATGCAGGAATTAATGATCCAAATAAACCTATTGCTTCGTTTTTGTA
TGTAGGTCCGACGGGAGTGGGTAAAACAGAGCTTGCTAAAGAGCTTGCACGACAGCTTTTTCAGGATGAAACTAAATTAC
TTCGTTTTGATATGTCAGAATACGAATCTCAGGTAGGAATTAATCGATTAATAGGGGTTCCTAATGGTTATAAAGATTCT
GAACACGGAGGGCTTCTTTCTAATGCAATCTTAGCGAACCCTCATGCTGTTGTTTTACTAGATGAAATTGAAAAAGCTGA
TCCAAAAGTAAGAGCTCTATTTCTACATATATTTGACGAAGGTTATTTTACGAGTGGTGTCGGCGAATTGGTTGATTGTC
GCAATTGTGTGTTTATTGCTACAACAAACCTTGCCAGCCAAGCCATCTTAAATATTCATCCTTTTTCTTATGAGAAAGCC
CTTGATACAATTGAGATTTATCTCATTAATGCTTTGACTCCAGAATTTTACAATCGAATTGAACCAGTCATTTTTCAAGG
GTTAGATCGGGAAATGATGAAAGAAATAGCCCAAATTAAGTTAAATCAATTAGCGCAAAATATTTTAAAACAAAAAAAAG
TTAAAGTTTATTTTGATGAATTCGTTGTAGACTATATTCAAACAAAGGGATATCATCCTGCGCTGGGCGCAAGACCTATG
CAAAGATTGATTAAAAATGAATTAACACCATTAATAGCAAAAACAATTTTGCATGCTCAGTATTTACCTGGTGATGTGAT
GCATATCTCTTATTTAGAGTCAGAATTGATTATCAGTCGGCTCGCAGAATCAGAAGCTAACGAAATTTTGAGTACTAATC
CTTAA

Upstream 100 bases:

>100_bases
AAGATCCTTTCAAAATCTGATTTTTACTAAATTTTTATCAATTGATATAAAATAATTAAACTGTTTAAACTAATTATTGA
ATATTAAAAAGGATTTAAAA

Downstream 100 bases:

>100_bases
AAATGAAAGAAACCAGCATTAAATTATCAATTTGGTGCAGACAGTTAAAATAGTGTTTTAAGCTAATTCTAAGTGGTAAC
ATACAAGTAAGATTAAAGTT

Product: putative endopeptidase Clp ATP-binding chain B (heat shock protein)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKKILLVISFLAVSLIKAENLSITTEEKELTLSLQEIDHLQAWWPWSSTPKVVNPKPLSPFNVLELENNLREKVIGQSQA
VKNTANALLCYYAGINDPNKPIASFLYVGPTGVGKTELAKELARQLFQDETKLLRFDMSEYESQVGINRLIGVPNGYKDS
EHGGLLSNAILANPHAVVLLDEIEKADPKVRALFLHIFDEGYFTSGVGELVDCRNCVFIATTNLASQAILNIHPFSYEKA
LDTIEIYLINALTPEFYNRIEPVIFQGLDREMMKEIAQIKLNQLAQNILKQKKVKVYFDEFVVDYIQTKGYHPALGARPM
QRLIKNELTPLIAKTILHAQYLPGDVMHISYLESELIISRLAESEANEILSTNP

Sequences:

>Translated_374_residues
MKKILLVISFLAVSLIKAENLSITTEEKELTLSLQEIDHLQAWWPWSSTPKVVNPKPLSPFNVLELENNLREKVIGQSQA
VKNTANALLCYYAGINDPNKPIASFLYVGPTGVGKTELAKELARQLFQDETKLLRFDMSEYESQVGINRLIGVPNGYKDS
EHGGLLSNAILANPHAVVLLDEIEKADPKVRALFLHIFDEGYFTSGVGELVDCRNCVFIATTNLASQAILNIHPFSYEKA
LDTIEIYLINALTPEFYNRIEPVIFQGLDREMMKEIAQIKLNQLAQNILKQKKVKVYFDEFVVDYIQTKGYHPALGARPM
QRLIKNELTPLIAKTILHAQYLPGDVMHISYLESELIISRLAESEANEILSTNP
>Mature_374_residues
MKKILLVISFLAVSLIKAENLSITTEEKELTLSLQEIDHLQAWWPWSSTPKVVNPKPLSPFNVLELENNLREKVIGQSQA
VKNTANALLCYYAGINDPNKPIASFLYVGPTGVGKTELAKELARQLFQDETKLLRFDMSEYESQVGINRLIGVPNGYKDS
EHGGLLSNAILANPHAVVLLDEIEKADPKVRALFLHIFDEGYFTSGVGELVDCRNCVFIATTNLASQAILNIHPFSYEKA
LDTIEIYLINALTPEFYNRIEPVIFQGLDREMMKEIAQIKLNQLAQNILKQKKVKVYFDEFVVDYIQTKGYHPALGARPM
QRLIKNELTPLIAKTILHAQYLPGDVMHISYLESELIISRLAESEANEILSTNP

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=307, Percent_Identity=31.5960912052117, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1788943, Length=314, Percent_Identity=36.9426751592357, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787109, Length=285, Percent_Identity=37.5438596491228, Blast_Score=181, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6320464, Length=302, Percent_Identity=37.4172185430464, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323002, Length=278, Percent_Identity=35.6115107913669, Blast_Score=185, Evalue=1e-47,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 42125; Mature: 42125

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLVISFLAVSLIKAENLSITTEEKELTLSLQEIDHLQAWWPWSSTPKVVNPKPLSP
CHHHHHHHHHHHHHHHHHCCCEEEECCHHHEEEHHHHHHHHHCCCCCCCCCCCCCCCCCC
FNVLELENNLREKVIGQSQAVKNTANALLCYYAGINDPNKPIASFLYVGPTGVGKTELAK
CHHHHHHHHHHHHHHCHHHHHHHHHHEEEEEEECCCCCCCHHHHEEEECCCCCCHHHHHH
ELARQLFQDETKLLRFDMSEYESQVGINRLIGVPNGYKDSEHGGLLSNAILANPHAVVLL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEEEE
DEIEKADPKVRALFLHIFDEGYFTSGVGELVDCRNCVFIATTNLASQAILNIHPFSYEKA
HHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEECCHHHHHHEECCCCCHHHH
LDTIEIYLINALTPEFYNRIEPVIFQGLDREMMKEIAQIKLNQLAQNILKQKKVKVYFDE
HHHEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVVDYIQTKGYHPALGARPMQRLIKNELTPLIAKTILHAQYLPGDVMHISYLESELIISR
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LAESEANEILSTNP
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKKILLVISFLAVSLIKAENLSITTEEKELTLSLQEIDHLQAWWPWSSTPKVVNPKPLSP
CHHHHHHHHHHHHHHHHHCCCEEEECCHHHEEEHHHHHHHHHCCCCCCCCCCCCCCCCCC
FNVLELENNLREKVIGQSQAVKNTANALLCYYAGINDPNKPIASFLYVGPTGVGKTELAK
CHHHHHHHHHHHHHHCHHHHHHHHHHEEEEEEECCCCCCCHHHHEEEECCCCCCHHHHHH
ELARQLFQDETKLLRFDMSEYESQVGINRLIGVPNGYKDSEHGGLLSNAILANPHAVVLL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEEEE
DEIEKADPKVRALFLHIFDEGYFTSGVGELVDCRNCVFIATTNLASQAILNIHPFSYEKA
HHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEECCHHHHHHEECCCCCHHHH
LDTIEIYLINALTPEFYNRIEPVIFQGLDREMMKEIAQIKLNQLAQNILKQKKVKVYFDE
HHHEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVVDYIQTKGYHPALGARPMQRLIKNELTPLIAKTILHAQYLPGDVMHISYLESELIISR
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LAESEANEILSTNP
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]