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The map label for this gene is ispF [H]

Identifier: 46445861

GI number: 46445861

Start: 323095

End: 323574

Strand: Reverse

Name: ispF [H]

Synonym: pc0227

Alternate gene names: 46445861

Gene position: 323574-323095 (Counterclockwise)

Preceding gene: 46445865

Following gene: 46445858

Centisome position: 13.4

GC content: 37.08

Gene sequence:

>480_bases
ATGACTGTGCGTACTGGTATTGGGCAAGATAGCCACCGTTTTTTACCTCAAGACTCATCTAAATTGTGCATTATTGGAGG
AATTATTTTTGATGATGCCCCAGGTTTTAATGCTAATTCTGATGGAGATGTCGTATTTCATGCAATTTGTAATGCCATTA
CATCCTTAACAGGAATTCTTATTCTAGGAAATATCGCTGATGATTTATGTCTGAAAGATGGGATTACTGATAGTGAAATC
TATTTAATCAAAGCGATGAAAACTTTAGAAAGGCAAAAAGTTACTCATGTTGCGATTACTATAGAAGGAAAAAAACCCAA
ATTTAAGCATCGTCTTCTTGAAATGCGCCAAAATATTGCTCGTGTCATGAACCTTGATATTACTCAGGTAGGAGTGACAG
CGACTTCAGGTGAAGGATTAACCGATTTTGGATGCGGAGATGGGGTTCAAGCATTTGCTATTGTCACAACGACTGAATAA

Upstream 100 bases:

>100_bases
CTTTAAAGAATTCCTGTGAGAAAATTCCGTTTAATTCAAAAAATTTTATGAAATTAAAACCCTTAGCTATGTAAATTTCT
GGTTATCTAAAGGAGATTTT

Downstream 100 bases:

>100_bases
TTAAAAAAATTAATTATTTTTTATGACTACTAAATTTCAAAAAAGCTTTCTATTTTTTTAAAAAAGATAATGTTTTTTGA
AATGATTTTATTTCGGAGAT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTVRTGIGQDSHRFLPQDSSKLCIIGGIIFDDAPGFNANSDGDVVFHAICNAITSLTGILILGNIADDLCLKDGITDSEI
YLIKAMKTLERQKVTHVAITIEGKKPKFKHRLLEMRQNIARVMNLDITQVGVTATSGEGLTDFGCGDGVQAFAIVTTTE

Sequences:

>Translated_159_residues
MTVRTGIGQDSHRFLPQDSSKLCIIGGIIFDDAPGFNANSDGDVVFHAICNAITSLTGILILGNIADDLCLKDGITDSEI
YLIKAMKTLERQKVTHVAITIEGKKPKFKHRLLEMRQNIARVMNLDITQVGVTATSGEGLTDFGCGDGVQAFAIVTTTE
>Mature_158_residues
TVRTGIGQDSHRFLPQDSSKLCIIGGIIFDDAPGFNANSDGDVVFHAICNAITSLTGILILGNIADDLCLKDGITDSEIY
LIKAMKTLERQKVTHVAITIEGKKPKFKHRLLEMRQNIARVMNLDITQVGVTATSGEGLTDFGCGDGVQAFAIVTTTE

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17124; Mature: 16993

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRTGIGQDSHRFLPQDSSKLCIIGGIIFDDAPGFNANSDGDVVFHAICNAITSLTGIL
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHE
ILGNIADDLCLKDGITDSEIYLIKAMKTLERQKVTHVAITIEGKKPKFKHRLLEMRQNIA
EECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHH
RVMNLDITQVGVTATSGEGLTDFGCGDGVQAFAIVTTTE
HHHCCCCHHEEEEEECCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
TVRTGIGQDSHRFLPQDSSKLCIIGGIIFDDAPGFNANSDGDVVFHAICNAITSLTGIL
CCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHE
ILGNIADDLCLKDGITDSEIYLIKAMKTLERQKVTHVAITIEGKKPKFKHRLLEMRQNIA
EECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHH
RVMNLDITQVGVTATSGEGLTDFGCGDGVQAFAIVTTTE
HHHCCCCHHEEEEEECCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]