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The map label for this gene is polA [H]

Identifier: 46445857

GI number: 46445857

Start: 315596

End: 318271

Strand: Reverse

Name: polA [H]

Synonym: pc0223

Alternate gene names: 46445857

Gene position: 318271-315596 (Counterclockwise)

Preceding gene: 46445858

Following gene: 46445856

Centisome position: 13.18

GC content: 34.64

Gene sequence:

>2676_bases
ATGGATAAACTCTATATTCTAGATGCCTCTGGCTACATTTATCGTTCTTATTTCGCGATTCGACAAATGACCAATGCCAG
AGGTGAATCTACCAATGCTCTTTTTGGTTTCATCCGTTCAGTTTTAAAATTAATTAAAGATTTTAATCCAACTCATCTTG
TAGCTGTTTTTGATGGACCTAACAACTCCCAAAAAAGAACAGAACTCTATCCTGCCTACAAGGCTCATCGAAAAGAGATG
CCTAAAGATTTGCTTTACCAAATTTTATGGTCGCAAAGATTTTGTCAACTGATGGGGATTCCAGAATTAATGGTTCCAGG
TGTTGAAGCTGATGATACAATGGGCAGCATTGCCAAATGGGCTGCAACCCTCAACACCACAGCGTATATATGTACTAGCG
ATAAAGATATGTGCCAACTTGTCAGCAACCAAATTTTGATTTTGAACACTTTTAAGGATAATTTAATTATTGATGCTAAT
GGTGTGAAAGAACAGTTTGGTGTGATGCCTGCACAAATGATTGACTACTTAGCAATAATAGGAGATGCATCCGACAACAT
TCCAGGCCTTACGGGCTTTGGACCTAAAACTGCTGCCGACTTACTAGAAAAATTTGGTTCATTAGATTATATTTTAGAGC
ATCCTTTGGAAGTTTCAGGCAAAAAAAAACAAGACACGCTAATATCTGAAAAAGAAAAAGTTTTGTTAAGTAAAAAACTT
GTTATTGTCGATACAACTGTTTCCTTTCCCAACCAAGAAGATTTTTTTAAATTAACATCCCCTGCTTATCAAAGCTTAAA
AGAATTTTACGCGGAAATGAATTTCTCTTCTTTAATTCGAGAGTTGGAAGCTGTAAAAAGTGGGATTAGTTTTTCAGACC
TAGAGAGTCAAAATGAGGTAGTTTATCAGCTTGTTAATGATGAAGAAACCTTAGTTAACCTTATTTCCTATCTATCGCAG
CAAAAAGAAATTTGTCTAGATACTGAAACAACGGATCTTCGTCCTTTAGAAGCCCAACTTGTCGGAATTGGTTTAGGTGT
AGAACCCAAAAAAGCTTGGTACATTCCCTTGAACGGGCAATTAAATGGTGATTTCGTTTTAAGTCAACTTAAACCACTAC
TTGAAAATCCTTCAATTGGTTTTTATGGACACAATTTTAAATACGATTATCATGTTTTAAGAAATCACAATATTTCTGTT
GCAAATATAAGTTTTGATACGATTTTAGCCTCTTATCTTCTCAATTCTCATAAAAGACAACATTCATTAGATCATTTGGC
TCTAGAATTATTTGATAAGGTTAAAATCGCTATACAAGAATTAATCGGGAAAGGAAAAAACCAGCTTAACATGAAGAATG
TGGCTTTAGATAAAATATGCCATTATTGTTGTGAAGATGTAGATTATACCATTAGACTTAAAAACATTTTGTTATCTCAA
TTAGAGGAGCGAAAATTAACTTCTCTTTTATTTGATTTAGAACTTCCTTTATTGTCCGTTCTTGCACAAATGGAAAGAAA
TGGGATTTTTATTGATACGGCTTATCTAAAACATTTATCCCAGTTTATTGGGCAAGAAATTCATTGCTTAGAACAAAATA
TTTATGCGTTAGCAGGAGAAATTTTTAACCTCAACTCTCCCAAACAACTCAGTCAAATTTTATTTCATAAACTCGGAATC
AAACCCCCTAAAAAAATTGCCACAGGTCACTCAACAAATGCGGAAGTTCTAGAAATTCTCAAACATGTTTATCCAATTGC
CGAAAAACTTCTTGAATATCGGACTTTAGAAAAACTTCGCTCAACTTATGTTAACTCATTACCCCTTCAAGTTAACGCTA
AAACTGGACGTATTCACTGTAATTTTAATCAGTCTATGGCAGCAACAGGTCGACTGTCTTGCCAAGACCCAAATTTACAA
AATATTCCTGTCCGCACTGAAGTCGGTCGTCAAATTAGAGAGGCATTTCGACCTGAAAAAGAAGATTGGAGCTATTTGTC
AGCAGACTATTCTCAAATTGAACTTCGTTTGCTTGCTCACTTAAGCGAAGATCCTGTGCTAATCAAAGCTTTCTTATCCA
ATGAAGATATTCACAAATATACCGCTTCTTTAATTTTTGATGTTCCTCTTCAACAAGTTTCCTCTGAACAACGTTATCAA
GCTAAAGCGGTTAATTTTGGTTTGATTTATGGACAACAAGCGTTTGGCTTAGCTCATGAACTAGGAATTGATACAAAAAC
TGCTGCTGCTTTTATTCAACGTTATTTTGAGCGTTATGGAAAGGTCAAAGAATTTTTAGAAGCTTGCAAACAATCAGCAA
GAGAGACTGGCAAGGCTGTAACAATGTATGGGAGGGAAAGACTGCTTCCAGAAATTAGAAGTCAGAATGCCATGATTCGA
GCAACAGCAGATCGATTTGCAGTAAATACCCCTATCCAAGGGACGCAAGCTGATTTGATTAAAATGGCCATGCTAAAAAT
CAATAAACTTCTTATACAAGAAAAGAAAAAAGGATTTATGATTTTACAAATTCATGATGAACTTATTTTTGAGGTTCCCA
ATCAAGAGTTAGAATCCATTTCTCATTTAGTTAAGAATACCATGGAAAATATTATAAATCTAAAGGTACCGTTGATTGTT
AATATCCATATTGGCAAAAATTGGAAAGAATGTTAA

Upstream 100 bases:

>100_bases
AACATGAAGCTTCTGTATCTCCTGTCATCAACCTTTTGTTGCAAAATCAATACCTTTACCTTTATTATCCCCAATAAATT
TAAGGCACTAATGATTTATC

Downstream 100 bases:

>100_bases
AATTAAGAAAAGTAGCCATAACAGGCGGTCTTTCATGTGGTAAATCTTCAGTTTGCCGTATCTTAAAAGAACTTGGGGCT
TACGCTGTAAGCGCTGACGA

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 891; Mature: 891

Protein sequence:

>891_residues
MDKLYILDASGYIYRSYFAIRQMTNARGESTNALFGFIRSVLKLIKDFNPTHLVAVFDGPNNSQKRTELYPAYKAHRKEM
PKDLLYQILWSQRFCQLMGIPELMVPGVEADDTMGSIAKWAATLNTTAYICTSDKDMCQLVSNQILILNTFKDNLIIDAN
GVKEQFGVMPAQMIDYLAIIGDASDNIPGLTGFGPKTAADLLEKFGSLDYILEHPLEVSGKKKQDTLISEKEKVLLSKKL
VIVDTTVSFPNQEDFFKLTSPAYQSLKEFYAEMNFSSLIRELEAVKSGISFSDLESQNEVVYQLVNDEETLVNLISYLSQ
QKEICLDTETTDLRPLEAQLVGIGLGVEPKKAWYIPLNGQLNGDFVLSQLKPLLENPSIGFYGHNFKYDYHVLRNHNISV
ANISFDTILASYLLNSHKRQHSLDHLALELFDKVKIAIQELIGKGKNQLNMKNVALDKICHYCCEDVDYTIRLKNILLSQ
LEERKLTSLLFDLELPLLSVLAQMERNGIFIDTAYLKHLSQFIGQEIHCLEQNIYALAGEIFNLNSPKQLSQILFHKLGI
KPPKKIATGHSTNAEVLEILKHVYPIAEKLLEYRTLEKLRSTYVNSLPLQVNAKTGRIHCNFNQSMAATGRLSCQDPNLQ
NIPVRTEVGRQIREAFRPEKEDWSYLSADYSQIELRLLAHLSEDPVLIKAFLSNEDIHKYTASLIFDVPLQQVSSEQRYQ
AKAVNFGLIYGQQAFGLAHELGIDTKTAAAFIQRYFERYGKVKEFLEACKQSARETGKAVTMYGRERLLPEIRSQNAMIR
ATADRFAVNTPIQGTQADLIKMAMLKINKLLIQEKKKGFMILQIHDELIFEVPNQELESISHLVKNTMENIINLKVPLIV
NIHIGKNWKEC

Sequences:

>Translated_891_residues
MDKLYILDASGYIYRSYFAIRQMTNARGESTNALFGFIRSVLKLIKDFNPTHLVAVFDGPNNSQKRTELYPAYKAHRKEM
PKDLLYQILWSQRFCQLMGIPELMVPGVEADDTMGSIAKWAATLNTTAYICTSDKDMCQLVSNQILILNTFKDNLIIDAN
GVKEQFGVMPAQMIDYLAIIGDASDNIPGLTGFGPKTAADLLEKFGSLDYILEHPLEVSGKKKQDTLISEKEKVLLSKKL
VIVDTTVSFPNQEDFFKLTSPAYQSLKEFYAEMNFSSLIRELEAVKSGISFSDLESQNEVVYQLVNDEETLVNLISYLSQ
QKEICLDTETTDLRPLEAQLVGIGLGVEPKKAWYIPLNGQLNGDFVLSQLKPLLENPSIGFYGHNFKYDYHVLRNHNISV
ANISFDTILASYLLNSHKRQHSLDHLALELFDKVKIAIQELIGKGKNQLNMKNVALDKICHYCCEDVDYTIRLKNILLSQ
LEERKLTSLLFDLELPLLSVLAQMERNGIFIDTAYLKHLSQFIGQEIHCLEQNIYALAGEIFNLNSPKQLSQILFHKLGI
KPPKKIATGHSTNAEVLEILKHVYPIAEKLLEYRTLEKLRSTYVNSLPLQVNAKTGRIHCNFNQSMAATGRLSCQDPNLQ
NIPVRTEVGRQIREAFRPEKEDWSYLSADYSQIELRLLAHLSEDPVLIKAFLSNEDIHKYTASLIFDVPLQQVSSEQRYQ
AKAVNFGLIYGQQAFGLAHELGIDTKTAAAFIQRYFERYGKVKEFLEACKQSARETGKAVTMYGRERLLPEIRSQNAMIR
ATADRFAVNTPIQGTQADLIKMAMLKINKLLIQEKKKGFMILQIHDELIFEVPNQELESISHLVKNTMENIINLKVPLIV
NIHIGKNWKEC
>Mature_891_residues
MDKLYILDASGYIYRSYFAIRQMTNARGESTNALFGFIRSVLKLIKDFNPTHLVAVFDGPNNSQKRTELYPAYKAHRKEM
PKDLLYQILWSQRFCQLMGIPELMVPGVEADDTMGSIAKWAATLNTTAYICTSDKDMCQLVSNQILILNTFKDNLIIDAN
GVKEQFGVMPAQMIDYLAIIGDASDNIPGLTGFGPKTAADLLEKFGSLDYILEHPLEVSGKKKQDTLISEKEKVLLSKKL
VIVDTTVSFPNQEDFFKLTSPAYQSLKEFYAEMNFSSLIRELEAVKSGISFSDLESQNEVVYQLVNDEETLVNLISYLSQ
QKEICLDTETTDLRPLEAQLVGIGLGVEPKKAWYIPLNGQLNGDFVLSQLKPLLENPSIGFYGHNFKYDYHVLRNHNISV
ANISFDTILASYLLNSHKRQHSLDHLALELFDKVKIAIQELIGKGKNQLNMKNVALDKICHYCCEDVDYTIRLKNILLSQ
LEERKLTSLLFDLELPLLSVLAQMERNGIFIDTAYLKHLSQFIGQEIHCLEQNIYALAGEIFNLNSPKQLSQILFHKLGI
KPPKKIATGHSTNAEVLEILKHVYPIAEKLLEYRTLEKLRSTYVNSLPLQVNAKTGRIHCNFNQSMAATGRLSCQDPNLQ
NIPVRTEVGRQIREAFRPEKEDWSYLSADYSQIELRLLAHLSEDPVLIKAFLSNEDIHKYTASLIFDVPLQQVSSEQRYQ
AKAVNFGLIYGQQAFGLAHELGIDTKTAAAFIQRYFERYGKVKEFLEACKQSARETGKAVTMYGRERLLPEIRSQNAMIR
ATADRFAVNTPIQGTQADLIKMAMLKINKLLIQEKKKGFMILQIHDELIFEVPNQELESISHLVKNTMENIINLKVPLIV
NIHIGKNWKEC

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=542, Percent_Identity=31.3653136531365, Blast_Score=255, Evalue=2e-67,
Organism=Homo sapiens, GI139394648, Length=531, Percent_Identity=29.0018832391714, Blast_Score=198, Evalue=2e-50,
Organism=Escherichia coli, GI1790294, Length=924, Percent_Identity=41.2337662337662, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI226510970, Length=204, Percent_Identity=34.8039215686275, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI86563272, Length=396, Percent_Identity=31.3131313131313, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI17933644, Length=429, Percent_Identity=31.9347319347319, Blast_Score=195, Evalue=1e-49,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 101231; Mature: 101231

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLYILDASGYIYRSYFAIRQMTNARGESTNALFGFIRSVLKLIKDFNPTHLVAVFDGP
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECC
NNSQKRTELYPAYKAHRKEMPKDLLYQILWSQRFCQLMGIPELMVPGVEADDTMGSIAKW
CCCCHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHH
AATLNTTAYICTSDKDMCQLVSNQILILNTFKDNLIIDANGVKEQFGVMPAQMIDYLAII
HHHHCCEEEEEECCHHHHHHHCCCEEEEEECCCCEEEECCCCHHHHCCCHHHHHHHHHHC
GDASDNIPGLTGFGPKTAADLLEKFGSLDYILEHPLEVSGKKKQDTLISEKEKVLLSKKL
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCE
VIVDTTVSFPNQEDFFKLTSPAYQSLKEFYAEMNFSSLIRELEAVKSGISFSDLESQNEV
EEEEEEECCCCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHCCCCCHH
VYQLVNDEETLVNLISYLSQQKEICLDTETTDLRPLEAQLVGIGLGVEPKKAWYIPLNGQ
HHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCC
LNGDFVLSQLKPLLENPSIGFYGHNFKYDYHVLRNHNISVANISFDTILASYLLNSHKRQ
CCHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEECCCEEEEECHHHHHHHHHHHHHHHHH
HSLDHLALELFDKVKIAIQELIGKGKNQLNMKNVALDKICHYCCEDVDYTIRLKNILLSQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
LEERKLTSLLFDLELPLLSVLAQMERNGIFIDTAYLKHLSQFIGQEIHCLEQNIYALAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHH
IFNLNSPKQLSQILFHKLGIKPPKKIATGHSTNAEVLEILKHVYPIAEKLLEYRTLEKLR
HCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
STYVNSLPLQVNAKTGRIHCNFNQSMAATGRLSCQDPNLQNIPVRTEVGRQIREAFRPEK
HHHHCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
EDWSYLSADYSQIELRLLAHLSEDPVLIKAFLSNEDIHKYTASLIFDVPLQQVSSEQRYQ
CCCHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHCCCHHHHH
AKAVNFGLIYGQQAFGLAHELGIDTKTAAAFIQRYFERYGKVKEFLEACKQSARETGKAV
HHHEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
TMYGRERLLPEIRSQNAMIRATADRFAVNTPIQGTQADLIKMAMLKINKLLIQEKKKGFM
EEHHHHHHHHHHHCCCCEEEEEHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
ILQIHDELIFEVPNQELESISHLVKNTMENIINLKVPLIVNIHIGKNWKEC
EEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MDKLYILDASGYIYRSYFAIRQMTNARGESTNALFGFIRSVLKLIKDFNPTHLVAVFDGP
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECC
NNSQKRTELYPAYKAHRKEMPKDLLYQILWSQRFCQLMGIPELMVPGVEADDTMGSIAKW
CCCCHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHH
AATLNTTAYICTSDKDMCQLVSNQILILNTFKDNLIIDANGVKEQFGVMPAQMIDYLAII
HHHHCCEEEEEECCHHHHHHHCCCEEEEEECCCCEEEECCCCHHHHCCCHHHHHHHHHHC
GDASDNIPGLTGFGPKTAADLLEKFGSLDYILEHPLEVSGKKKQDTLISEKEKVLLSKKL
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCE
VIVDTTVSFPNQEDFFKLTSPAYQSLKEFYAEMNFSSLIRELEAVKSGISFSDLESQNEV
EEEEEEECCCCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHCCCCCHH
VYQLVNDEETLVNLISYLSQQKEICLDTETTDLRPLEAQLVGIGLGVEPKKAWYIPLNGQ
HHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCC
LNGDFVLSQLKPLLENPSIGFYGHNFKYDYHVLRNHNISVANISFDTILASYLLNSHKRQ
CCHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEECCCEEEEECHHHHHHHHHHHHHHHHH
HSLDHLALELFDKVKIAIQELIGKGKNQLNMKNVALDKICHYCCEDVDYTIRLKNILLSQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
LEERKLTSLLFDLELPLLSVLAQMERNGIFIDTAYLKHLSQFIGQEIHCLEQNIYALAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHH
IFNLNSPKQLSQILFHKLGIKPPKKIATGHSTNAEVLEILKHVYPIAEKLLEYRTLEKLR
HCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
STYVNSLPLQVNAKTGRIHCNFNQSMAATGRLSCQDPNLQNIPVRTEVGRQIREAFRPEK
HHHHCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
EDWSYLSADYSQIELRLLAHLSEDPVLIKAFLSNEDIHKYTASLIFDVPLQQVSSEQRYQ
CCCHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHCCCHHHHH
AKAVNFGLIYGQQAFGLAHELGIDTKTAAAFIQRYFERYGKVKEFLEACKQSARETGKAV
HHHEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
TMYGRERLLPEIRSQNAMIRATADRFAVNTPIQGTQADLIKMAMLKINKLLIQEKKKGFM
EEHHHHHHHHHHHCCCCEEEEEHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
ILQIHDELIFEVPNQELESISHLVKNTMENIINLKVPLIVNIHIGKNWKEC
EEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6276402; 8346018; 9278503; 6183253; 6302278; 7035456; 3883192; 1989886; 8469987; 8260491; 9514742; 9888810; 10588690; 8442659 [H]