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The map label for this gene is birA [H]

Identifier: 46445799

GI number: 46445799

Start: 244095

End: 244775

Strand: Reverse

Name: birA [H]

Synonym: pc0165

Alternate gene names: 46445799

Gene position: 244775-244095 (Counterclockwise)

Preceding gene: 46445809

Following gene: 46445798

Centisome position: 10.14

GC content: 33.33

Gene sequence:

>681_bases
GTGGATCAAATAGATCCTGAGGGTGTCACTCTCATTTCTGCATCCGAACAAACTTTAGGAAGAGGTCGCTTTAAAAGACG
CTGGCAATCTCCTATTAATCTAAATATTTATGCAACTTTTTGTTTTTATATTGATTCTAATCGTTCCGATTTTGGACATC
TTCCACAACTTTTAGCTTTAGCTGCCATCCAAGTTCTGGAGGATTTAAATTTTCATCCAGCTTTAAAATGGCCTAACGAC
ATTTTGCTCAACGGAAAAAAAGTCGCGGGAATTTTATGTGAAACAATGATGTTAGAGCAAAAACGGTATATGATTAACGG
CATAGGACTAAACGTTAATATGCCGACAGAGCTGCTTAACAAAATTGATCGGCCTGCCACTTCTCTTTTTAAAGAGCGAG
GACATGAATTTGATCTTAATATTCTTTTAAAACAATTAGACGTCAAGTTTACAAGTTATTTATCTCATTTTCTAAAGCAT
GGTTTTGATGCATTTTTTCCTTCTTTTCAACTACGTTCAACCTTTAAACAAGGTCAATTTATTCAATTTAACCACAATCA
AACCCTACTTGAAGGAATTTTTGAAAGTTTACATCCAGATGGCTCAGTACAAATTAAATTATTGGATGGAACTTCAAAAA
AATTTTATTCTGGAGAATTTATGTTTGAAGATTTCATATAA

Upstream 100 bases:

>100_bases
TTTTAATGATCATCTATTTTGTAAAAACCTCATGAAATCATCTTGCTTGCATTACCATTTTGAAACCATAGGTTCCACCA
ACACTTGGGCAAAAGAAAAT

Downstream 100 bases:

>100_bases
ATTTTATAAAGACATTAAAAACTAATTAAATAAAAGTTTTATTTATAATAAACAAAACAAATTTGACAATTTAGTTATTT
AAGAATAAAATATTTTCTTG

Product: putative biotin-[acetyl-CoA-carboxylase] ligase/biotin repressor (bifunctional)

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MDQIDPEGVTLISASEQTLGRGRFKRRWQSPINLNIYATFCFYIDSNRSDFGHLPQLLALAAIQVLEDLNFHPALKWPND
ILLNGKKVAGILCETMMLEQKRYMINGIGLNVNMPTELLNKIDRPATSLFKERGHEFDLNILLKQLDVKFTSYLSHFLKH
GFDAFFPSFQLRSTFKQGQFIQFNHNQTLLEGIFESLHPDGSVQIKLLDGTSKKFYSGEFMFEDFI

Sequences:

>Translated_226_residues
MDQIDPEGVTLISASEQTLGRGRFKRRWQSPINLNIYATFCFYIDSNRSDFGHLPQLLALAAIQVLEDLNFHPALKWPND
ILLNGKKVAGILCETMMLEQKRYMINGIGLNVNMPTELLNKIDRPATSLFKERGHEFDLNILLKQLDVKFTSYLSHFLKH
GFDAFFPSFQLRSTFKQGQFIQFNHNQTLLEGIFESLHPDGSVQIKLLDGTSKKFYSGEFMFEDFI
>Mature_226_residues
MDQIDPEGVTLISASEQTLGRGRFKRRWQSPINLNIYATFCFYIDSNRSDFGHLPQLLALAAIQVLEDLNFHPALKWPND
ILLNGKKVAGILCETMMLEQKRYMINGIGLNVNMPTELLNKIDRPATSLFKERGHEFDLNILLKQLDVKFTSYLSHFLKH
GFDAFFPSFQLRSTFKQGQFIQFNHNQTLLEGIFESLHPDGSVQIKLLDGTSKKFYSGEFMFEDFI

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 26045; Mature: 26045

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQIDPEGVTLISASEQTLGRGRFKRRWQSPINLNIYATFCFYIDSNRSDFGHLPQLLAL
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHH
AAIQVLEDLNFHPALKWPNDILLNGKKVAGILCETMMLEQKRYMINGIGLNVNMPTELLN
HHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHH
KIDRPATSLFKERGHEFDLNILLKQLDVKFTSYLSHFLKHGFDAFFPSFQLRSTFKQGQF
HHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCE
IQFNHNQTLLEGIFESLHPDGSVQIKLLDGTSKKFYSGEFMFEDFI
EEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCEEEECCC
>Mature Secondary Structure
MDQIDPEGVTLISASEQTLGRGRFKRRWQSPINLNIYATFCFYIDSNRSDFGHLPQLLAL
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHH
AAIQVLEDLNFHPALKWPNDILLNGKKVAGILCETMMLEQKRYMINGIGLNVNMPTELLN
HHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHH
KIDRPATSLFKERGHEFDLNILLKQLDVKFTSYLSHFLKHGFDAFFPSFQLRSTFKQGQF
HHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCE
IQFNHNQTLLEGIFESLHPDGSVQIKLLDGTSKKFYSGEFMFEDFI
EEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]