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The map label for this gene is rfbD [H]

Identifier: 46445759

GI number: 46445759

Start: 194956

End: 195840

Strand: Direct

Name: rfbD [H]

Synonym: pc0125

Alternate gene names: 46445759

Gene position: 194956-195840 (Clockwise)

Preceding gene: 46445758

Following gene: 46445760

Centisome position: 8.07

GC content: 37.06

Gene sequence:

>885_bases
ATGAAAAAGATTTGGGTATGTGGCGCATCGGGTATGCTTGGTTCTCATTTCAAACGTTTATTAAACAAACGGCAACTGTC
TTTTGTTGCCAATGATGATAAAAAAATTGATATTACCAATTTAGAAGCCGTTTTAGATTTTGTAAGAACAGAACAAATCA
CTCATATCATCAATTGTGCGGCTTACACAAAAGTTGATAAAGCTGAAACAGATCTCAAACAAGCTTATCTCGTCAATGCC
TGTGGACCACACCATCTTGGAATTGCAGCTCGGCACCAGAATGCGCATGTCATTCATTTCTCGACTGATTATGTGTTTGA
TGGAAAAGAGAACTTGCCTTATACCGAAGAACATGCTTGCGCTCCCATTGGAGCTTATGGGATAAGTAAATTAGCTGGAG
AAATCAAACTCTTAGATGAATTTGATCGCTCTTGTGTCATTCGCACATCTTGGTTATTTGGCTTACCAGGCAAAAATTTT
GTTGAAACAATGTTAAGATTGATGAATGAAAAAGCACAGATCAAGATTGTTTGCGATCAGATGGGAAGACCGACTTATGC
GCAAGATTTAGCTGAAGTTGCTTTACAATTTTTGGATAAGTCGGGAATTTATCACTTTGCTAACTCTTCTGAAACTAACT
GGTATGAGTTTGCTAAAGAGATTTATAGACAAGGAAAGGAATTTCAATTGATTCAAAGGGATTGTCAAATAGAACCTATT
ATGACTCATGAATATCCGACACAGGCCAAACGACCTGCTTATTCTACTTTAAATACTCAAAAAATAGAGTCTGTGCTTAG
ATGGAAACCACGTCCCTGGCAAGAGGCATTAAAAGATTATTTGACGATATATAAAAATTTCCAAGATAGGCAACAGGTGA
GCTAA

Upstream 100 bases:

>100_bases
TAAATATTCAATGGCCTATAGAGCAACCAATAGTTTCTGAAAGAGATAAACAATCTCCTTCCTTTCGTGAAAGCATGCTT
CAATTAACTGAGATTAGAAA

Downstream 100 bases:

>100_bases
TTATGCAACCAAGAAAAGTTCAAAATATTCTCGTGACTGGAGGAGCAGGTTTTATTGGATCAGCTTTTATTCGTTATTTA
CTTGCCCCTGAAACTGAATT

Product: putative dTDP-4-keto-L-rhamnose reductase, (TDP-rhamnose synthetase), rfbD

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKKIWVCGASGMLGSHFKRLLNKRQLSFVANDDKKIDITNLEAVLDFVRTEQITHIINCAAYTKVDKAETDLKQAYLVNA
CGPHHLGIAARHQNAHVIHFSTDYVFDGKENLPYTEEHACAPIGAYGISKLAGEIKLLDEFDRSCVIRTSWLFGLPGKNF
VETMLRLMNEKAQIKIVCDQMGRPTYAQDLAEVALQFLDKSGIYHFANSSETNWYEFAKEIYRQGKEFQLIQRDCQIEPI
MTHEYPTQAKRPAYSTLNTQKIESVLRWKPRPWQEALKDYLTIYKNFQDRQQVS

Sequences:

>Translated_294_residues
MKKIWVCGASGMLGSHFKRLLNKRQLSFVANDDKKIDITNLEAVLDFVRTEQITHIINCAAYTKVDKAETDLKQAYLVNA
CGPHHLGIAARHQNAHVIHFSTDYVFDGKENLPYTEEHACAPIGAYGISKLAGEIKLLDEFDRSCVIRTSWLFGLPGKNF
VETMLRLMNEKAQIKIVCDQMGRPTYAQDLAEVALQFLDKSGIYHFANSSETNWYEFAKEIYRQGKEFQLIQRDCQIEPI
MTHEYPTQAKRPAYSTLNTQKIESVLRWKPRPWQEALKDYLTIYKNFQDRQQVS
>Mature_294_residues
MKKIWVCGASGMLGSHFKRLLNKRQLSFVANDDKKIDITNLEAVLDFVRTEQITHIINCAAYTKVDKAETDLKQAYLVNA
CGPHHLGIAARHQNAHVIHFSTDYVFDGKENLPYTEEHACAPIGAYGISKLAGEIKLLDEFDRSCVIRTSWLFGLPGKNF
VETMLRLMNEKAQIKIVCDQMGRPTYAQDLAEVALQFLDKSGIYHFANSSETNWYEFAKEIYRQGKEFQLIQRDCQIEPI
MTHEYPTQAKRPAYSTLNTQKIESVLRWKPRPWQEALKDYLTIYKNFQDRQQVS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=299, Percent_Identity=26.7558528428094, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI33519455, Length=299, Percent_Identity=26.7558528428094, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1788352, Length=289, Percent_Identity=35.9861591695502, Blast_Score=177, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 33902; Mature: 33902

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIWVCGASGMLGSHFKRLLNKRQLSFVANDDKKIDITNLEAVLDFVRTEQITHIINCA
CCEEEEECCCCHHHHHHHHHHHHHHEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
AYTKVDKAETDLKQAYLVNACGPHHLGIAARHQNAHVIHFSTDYVFDGKENLPYTEEHAC
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCEEECCCCCCCCCCCCCC
APIGAYGISKLAGEIKLLDEFDRSCVIRTSWLFGLPGKNFVETMLRLMNEKAQIKIVCDQ
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEHH
MGRPTYAQDLAEVALQFLDKSGIYHFANSSETNWYEFAKEIYRQGKEFQLIQRDCQIEPI
CCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCE
MTHEYPTQAKRPAYSTLNTQKIESVLRWKPRPWQEALKDYLTIYKNFQDRQQVS
ECCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHCCC
>Mature Secondary Structure
MKKIWVCGASGMLGSHFKRLLNKRQLSFVANDDKKIDITNLEAVLDFVRTEQITHIINCA
CCEEEEECCCCHHHHHHHHHHHHHHEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
AYTKVDKAETDLKQAYLVNACGPHHLGIAARHQNAHVIHFSTDYVFDGKENLPYTEEHAC
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCEEECCCCCCCCCCCCCC
APIGAYGISKLAGEIKLLDEFDRSCVIRTSWLFGLPGKNFVETMLRLMNEKAQIKIVCDQ
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEHH
MGRPTYAQDLAEVALQFLDKSGIYHFANSSETNWYEFAKEIYRQGKEFQLIQRDCQIEPI
CCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCE
MTHEYPTQAKRPAYSTLNTQKIESVLRWKPRPWQEALKDYLTIYKNFQDRQQVS
ECCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]