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The map label for this gene is rfbB [H]

Identifier: 46445760

GI number: 46445760

Start: 195843

End: 196901

Strand: Direct

Name: rfbB [H]

Synonym: pc0126

Alternate gene names: 46445760

Gene position: 195843-196901 (Clockwise)

Preceding gene: 46445759

Following gene: 46445766

Centisome position: 8.11

GC content: 36.26

Gene sequence:

>1059_bases
ATGCAACCAAGAAAAGTTCAAAATATTCTCGTGACTGGAGGAGCAGGTTTTATTGGATCAGCTTTTATTCGTTATTTACT
TGCCCCTGAAACTGAATTTAAAGGAACTTGTATCAATTTTGATGCTTTAACATATGCGGGAAATCTAGAAAATTTAGCTT
CCCTTTCAAGCGATCCTCGTTATATTTTCGAACAAGGCAATATCTGTAATGAAGCATTTATTGAACATGTCTGTCAAGAG
CATGCAATTGATACAATTATTCACTTTGCGGCAGAAAGTCATGTAGATCGTAGTATTTTAGGGCCAAAAGTGTTTATTGA
AACAAATATTTTAGGTACTTTTTCTTTATTAGAAGTGGTGAGAAAAAATCCTCACATTCATTTTCACCACGTTTCAACAG
ATGAAGTATATGGAACATTAGGGGCAGAAGGTTATTTTACAGAAGAAACAGCTTATCGACCTAACTCTCCTTACTCGGCT
TCGAAGGCTTCATCTGACCATCTTGTTCGCGCTTATCATCATACCTATCATTTATCCACTTGCATTTCTAATTGTAGTAA
CAATTATGGACCTTATCATTTCCCTGAAAAATTGATTCCAGTCATGATTTTGAACTGTTTGGATCGTAAACCCTTACCTG
TCTATGGACAGGGAGTTAATGTGCGTGATTGGTTGTATGTAGAAGACCACGCTAAGGCTCTTTACTTATTGCTTCAAAAA
GGAAGAAGTGGGGAAACTTACAATATTGGTGGAGAAGCAGAATGGCGAAATATTGATTTGATACATGAAATCATCAGACA
AATTGCAATTACTCAGCAAATAGAAGTTTCTGAACTTGAAAAGCTCATCACTTATGTTAAAGATCGGCCTGGCCATGATT
TGCGTTATGCTATTGATTGTTCTAAAATCAAAAATGAGTTTGGCTGGTCTCCTTCCCTCCGTTTTGAAGAAGGTCTCCAT
AAGACTATTCAATGGTACATACAAAATGAACAATGGGTAAAAAATATCCAAACGGGTAGTTATCGAAGATGGATAGAGCT
TAATTACGCCGATCGTTAA

Upstream 100 bases:

>100_bases
AGTCTGTGCTTAGATGGAAACCACGTCCCTGGCAAGAGGCATTAAAAGATTATTTGACGATATATAAAAATTTCCAAGAT
AGGCAACAGGTGAGCTAATT

Downstream 100 bases:

>100_bases
ATGAAGAGACTTTTTTTCGAAAGAGATCCCATTAATCCATGGCGACTTGCTGAGTTTTATTTGTGGTATTTTTTTTGCAT
AACCAAATTAAAGGAATGCA

Product: dTDP-glucose 4,6-dehydratase, rfbB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MQPRKVQNILVTGGAGFIGSAFIRYLLAPETEFKGTCINFDALTYAGNLENLASLSSDPRYIFEQGNICNEAFIEHVCQE
HAIDTIIHFAAESHVDRSILGPKVFIETNILGTFSLLEVVRKNPHIHFHHVSTDEVYGTLGAEGYFTEETAYRPNSPYSA
SKASSDHLVRAYHHTYHLSTCISNCSNNYGPYHFPEKLIPVMILNCLDRKPLPVYGQGVNVRDWLYVEDHAKALYLLLQK
GRSGETYNIGGEAEWRNIDLIHEIIRQIAITQQIEVSELEKLITYVKDRPGHDLRYAIDCSKIKNEFGWSPSLRFEEGLH
KTIQWYIQNEQWVKNIQTGSYRRWIELNYADR

Sequences:

>Translated_352_residues
MQPRKVQNILVTGGAGFIGSAFIRYLLAPETEFKGTCINFDALTYAGNLENLASLSSDPRYIFEQGNICNEAFIEHVCQE
HAIDTIIHFAAESHVDRSILGPKVFIETNILGTFSLLEVVRKNPHIHFHHVSTDEVYGTLGAEGYFTEETAYRPNSPYSA
SKASSDHLVRAYHHTYHLSTCISNCSNNYGPYHFPEKLIPVMILNCLDRKPLPVYGQGVNVRDWLYVEDHAKALYLLLQK
GRSGETYNIGGEAEWRNIDLIHEIIRQIAITQQIEVSELEKLITYVKDRPGHDLRYAIDCSKIKNEFGWSPSLRFEEGLH
KTIQWYIQNEQWVKNIQTGSYRRWIELNYADR
>Mature_352_residues
MQPRKVQNILVTGGAGFIGSAFIRYLLAPETEFKGTCINFDALTYAGNLENLASLSSDPRYIFEQGNICNEAFIEHVCQE
HAIDTIIHFAAESHVDRSILGPKVFIETNILGTFSLLEVVRKNPHIHFHHVSTDEVYGTLGAEGYFTEETAYRPNSPYSA
SKASSDHLVRAYHHTYHLSTCISNCSNNYGPYHFPEKLIPVMILNCLDRKPLPVYGQGVNVRDWLYVEDHAKALYLLLQK
GRSGETYNIGGEAEWRNIDLIHEIIRQIAITQQIEVSELEKLITYVKDRPGHDLRYAIDCSKIKNEFGWSPSLRFEEGLH
KTIQWYIQNEQWVKNIQTGSYRRWIELNYADR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=334, Percent_Identity=37.4251497005988, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=24.3323442136499, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=55.7142857142857, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=53.5911602209945, Blast_Score=393, Evalue=1e-111,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=24.7863247863248, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788366, Length=348, Percent_Identity=23.5632183908046, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=327, Percent_Identity=37.3088685015291, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=335, Percent_Identity=33.4328358208955, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17539532, Length=326, Percent_Identity=26.0736196319018, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=26.8361581920904, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=26.685393258427, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=355, Percent_Identity=25.0704225352113, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=26.3636363636364, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19923002, Length=360, Percent_Identity=25.5555555555556, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24158427, Length=337, Percent_Identity=25.5192878338279, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40384; Mature: 40384

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPRKVQNILVTGGAGFIGSAFIRYLLAPETEFKGTCINFDALTYAGNLENLASLSSDPR
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCHHEECCCHHHHHHCCCCCC
YIFEQGNICNEAFIEHVCQEHAIDTIIHFAAESHVDRSILGPKVFIETNILGTFSLLEVV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCHHHHHHHHHH
RKNPHIHFHHVSTDEVYGTLGAEGYFTEETAYRPNSPYSASKASSDHLVRAYHHTYHLST
HCCCCEEEEECCHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
CISNCSNNYGPYHFPEKLIPVMILNCLDRKPLPVYGQGVNVRDWLYVEDHAKALYLLLQK
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHC
GRSGETYNIGGEAEWRNIDLIHEIIRQIAITQQIEVSELEKLITYVKDRPGHDLRYAIDC
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEH
SKIKNEFGWSPSLRFEEGLHKTIQWYIQNEQWVKNIQTGSYRRWIELNYADR
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MQPRKVQNILVTGGAGFIGSAFIRYLLAPETEFKGTCINFDALTYAGNLENLASLSSDPR
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCHHEECCCHHHHHHCCCCCC
YIFEQGNICNEAFIEHVCQEHAIDTIIHFAAESHVDRSILGPKVFIETNILGTFSLLEVV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCHHHHHHHHHH
RKNPHIHFHHVSTDEVYGTLGAEGYFTEETAYRPNSPYSASKASSDHLVRAYHHTYHLST
HCCCCEEEEECCHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
CISNCSNNYGPYHFPEKLIPVMILNCLDRKPLPVYGQGVNVRDWLYVEDHAKALYLLLQK
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHC
GRSGETYNIGGEAEWRNIDLIHEIIRQIAITQQIEVSELEKLITYVKDRPGHDLRYAIDC
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEH
SKIKNEFGWSPSLRFEEGLHKTIQWYIQNEQWVKNIQTGSYRRWIELNYADR
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]