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The map label for this gene is proB

Identifier: 45658605

GI number: 45658605

Start: 3378598

End: 3379470

Strand: Reverse

Name: proB

Synonym: LIC12772

Alternate gene names: 45658605

Gene position: 3379470-3378598 (Counterclockwise)

Preceding gene: 45658606

Following gene: 45658604

Centisome position: 79.01

GC content: 39.18

Gene sequence:

>873_bases
ATGAAACCGGAACGAAACTCACTTTCTGAAAAGATCCGTAACGCTGAAAAGATCGTTATCAAAGTAGGTTCGGCAAGGCT
TTCTGGACTTCCGTCGGAAGTAAACGATTTTCTATTTCAATTGGTAAGTGATATTCGTCATCTTAGAGATTTAGGAAAAA
AGGTGATTTTAGTCTCTTCAGGAGCGATTGCAAGAGGAAGACTTTTGTTAAGCGAACTTCCCTCTACAATTTCTTCGGGG
GATTCACTTGCCGAAAAACAAGCGTTAGCCGCAATGGGGCAAAATAGGCTTGTCAATCTCTACGATAGTTTTTTTTCCAA
AGTCAATTTGAGCATCGCACAGATCCTTTTTGGTGTTTTGGATTTGGAATCCAAAGAAGGTTATAAAAATCTAAAGAACA
CGTTTACTCAACTTGTAGAATGGGGGATACTTCCAGTAGTTAACGAAAATGATTCCGTTGCAACCGAAGAAGTAAAGTTT
GGAGACAACGATATGCTTTCCGCTTTGGTGAGTTTGATCGTAGAAGCCGATCTTTTGATTATACTTACAGGTGTGGATGG
TTTTTTAAAAGAAGAGAAAGTTGTACCTTTTCTGGAGAAAATTTCTAAAGAAGATTTAGGTCTTGCTGGGGGTCCTAGTG
GACCTGGCACAGGCGGAATGTTCACTAAACTTAAATCTGCGGGTTTATTATCTGAGGCTGGGATTCCGACCGCGATCCTA
AACGGAAAAAAAATGCATGTGATTCGTGAATTTTTAGAAAAAAATTCAATAGGAACATTAGTTGCTCCTTCCGGAAACCG
CGTTTTTTCTGAAGAGGACGTAAAAGAAATCATCCGTAAAAATAGAAACGGAAACGGAGGAAATAGTTTATGA

Upstream 100 bases:

>100_bases
TTCAAAAATGAAATCGAAAAAATTCTAAATCCAATCAAAGATACAAAGCCTATCTCTTTGGATGAATCAGATATTTTCGA
AAGTTGAAGGATAAAGTAGA

Downstream 100 bases:

>100_bases
AAGAAATTGAATATGTACAGGATTTATGTTCTCGAGCCAAGAAGGCTTCAAAAGTTCTGAAACAACTTTCTTCTTCCAAA
AAAAATAAAATTCTTTTGAG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKPERNSLSEKIRNAEKIVIKVGSARLSGLPSEVNDFLFQLVSDIRHLRDLGKKVILVSSGAIARGRLLLSELPSTISSG
DSLAEKQALAAMGQNRLVNLYDSFFSKVNLSIAQILFGVLDLESKEGYKNLKNTFTQLVEWGILPVVNENDSVATEEVKF
GDNDMLSALVSLIVEADLLIILTGVDGFLKEEKVVPFLEKISKEDLGLAGGPSGPGTGGMFTKLKSAGLLSEAGIPTAIL
NGKKMHVIREFLEKNSIGTLVAPSGNRVFSEEDVKEIIRKNRNGNGGNSL

Sequences:

>Translated_290_residues
MKPERNSLSEKIRNAEKIVIKVGSARLSGLPSEVNDFLFQLVSDIRHLRDLGKKVILVSSGAIARGRLLLSELPSTISSG
DSLAEKQALAAMGQNRLVNLYDSFFSKVNLSIAQILFGVLDLESKEGYKNLKNTFTQLVEWGILPVVNENDSVATEEVKF
GDNDMLSALVSLIVEADLLIILTGVDGFLKEEKVVPFLEKISKEDLGLAGGPSGPGTGGMFTKLKSAGLLSEAGIPTAIL
NGKKMHVIREFLEKNSIGTLVAPSGNRVFSEEDVKEIIRKNRNGNGGNSL
>Mature_290_residues
MKPERNSLSEKIRNAEKIVIKVGSARLSGLPSEVNDFLFQLVSDIRHLRDLGKKVILVSSGAIARGRLLLSELPSTISSG
DSLAEKQALAAMGQNRLVNLYDSFFSKVNLSIAQILFGVLDLESKEGYKNLKNTFTQLVEWGILPVVNENDSVATEEVKF
GDNDMLSALVSLIVEADLLIILTGVDGFLKEEKVVPFLEKISKEDLGLAGGPSGPGTGGMFTKLKSAGLLSEAGIPTAIL
NGKKMHVIREFLEKNSIGTLVAPSGNRVFSEEDVKEIIRKNRNGNGGNSL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=300, Percent_Identity=29.3333333333333, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI21361368, Length=302, Percent_Identity=29.1390728476821, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786437, Length=259, Percent_Identity=39.7683397683398, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17569967, Length=295, Percent_Identity=31.1864406779661, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17569969, Length=295, Percent_Identity=31.1864406779661, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320506, Length=238, Percent_Identity=40.3361344537815, Blast_Score=142, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321822, Length=251, Percent_Identity=35.0597609561753, Blast_Score=129, Evalue=5e-31,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=29.9651567944251, Blast_Score=111, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_LEPIC (Q72NQ8)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002691.1
- ProteinModelPortal:   Q72NQ8
- SMR:   Q72NQ8
- GeneID:   2770261
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12772
- HOGENOM:   HBG507643
- OMA:   LINTIDP
- ProtClustDB:   PRK12314
- BioCyc:   LINT267671:LIC_12772-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.11

Molecular weight: Translated: 31308; Mature: 31308

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS00327 BACTERIAL_OPSIN_RET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPERNSLSEKIRNAEKIVIKVGSARLSGLPSEVNDFLFQLVSDIRHLRDLGKKVILVSS
CCCCHHHHHHHHHCHHEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
GAIARGRLLLSELPSTISSGDSLAEKQALAAMGQNRLVNLYDSFFSKVNLSIAQILFGVL
CCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLESKEGYKNLKNTFTQLVEWGILPVVNENDSVATEEVKFGDNDMLSALVSLIVEADLLI
HCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCEE
ILTGVDGFLKEEKVVPFLEKISKEDLGLAGGPSGPGTGGMFTKLKSAGLLSEAGIPTAIL
EEECCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHCCCCHHHH
NGKKMHVIREFLEKNSIGTLVAPSGNRVFSEEDVKEIIRKNRNGNGGNSL
CCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKPERNSLSEKIRNAEKIVIKVGSARLSGLPSEVNDFLFQLVSDIRHLRDLGKKVILVSS
CCCCHHHHHHHHHCHHEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
GAIARGRLLLSELPSTISSGDSLAEKQALAAMGQNRLVNLYDSFFSKVNLSIAQILFGVL
CCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLESKEGYKNLKNTFTQLVEWGILPVVNENDSVATEEVKFGDNDMLSALVSLIVEADLLI
HCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCEE
ILTGVDGFLKEEKVVPFLEKISKEDLGLAGGPSGPGTGGMFTKLKSAGLLSEAGIPTAIL
EEECCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHCCCCHHHH
NGKKMHVIREFLEKNSIGTLVAPSGNRVFSEEDVKEIIRKNRNGNGGNSL
CCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA