Definition Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 chromosome chromosome I, complete sequence.
Accession NC_005823
Length 4,277,185

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The map label for this gene is proA

Identifier: 45658604

GI number: 45658604

Start: 3377351

End: 3378601

Strand: Reverse

Name: proA

Synonym: LIC12771

Alternate gene names: 45658604

Gene position: 3378601-3377351 (Counterclockwise)

Preceding gene: 45658605

Following gene: 45658603

Centisome position: 78.99

GC content: 38.85

Gene sequence:

>1251_bases
ATGAAAGAAATTGAATATGTACAGGATTTATGTTCTCGAGCCAAGAAGGCTTCAAAAGTTCTGAAACAACTTTCTTCTTC
CAAAAAAAATAAAATTCTTTTGAGTTTAGCTGATCTATTAGAAAAACGTAAAGCGGAAATACTTTTGGCTAATGAATTGG
ACCTGAAGGATGGAAAGGAGAAAAAACTTTCTTCTGCATTGATGGATCGTTTGCTTTTGAACGAAAAAAGAATTTTTTCT
ATGGCTTCTGCGGTTCGTGAGATCGCAGCGCTTCCAGATCCTATCGGAGAGGTGACGAGAGGTATAACCCTCCCAAACGG
TCTAGAACTCGTAACTCGGAGAGTTCCGCTTGGGGTAGTGATGGTCATCTACGAATCTCGTCCAAACGTAACTATCGACG
TAGGCGCGCTTTCTTTTAAATCGGGAAATGCTTGTATTCTTAGAGGCGGAAGTGAGGCGTTTTATTCCAATGAAATCCTA
ATAAAGTTATTTCATGAAATTCTAATAAAAGAAGAAATTGATATAGGCTCTGTCGTTTTTGTGGATAAAACGGATCGTTC
TTTTATGATTCCTTTTTTTCAGCAGACTTCTTTAATAGACATCGTGGTTCCTAGAGGAGGAGAGGGATTGATTCGGTTTG
TTTCGGAAAATTCTAAAATTCCTGTCGTCAAACATGATAAAGGAGTTTGTAATCTTTATATCGACCAAGATGCAGATCCA
GAAAAAGTAATTCCAATCGTAATCAATTCTAAGGTGCAAAGGCCTGGGGTATGTAATTCTACAGAGAATTTAATACTTCA
TAACGGTTATCCATTTAGAAAGGAATTATTAGAGGCGCTTGCAAAAGAAGGTGTGGAACTATTACTAGATCCTTCCTCTC
TCGCTTTGTATCCAAAGGGAAAACCGGTTAAACAACAAGATTATCTAGAAGAATTTTTAGATCTTCGTCTTTCCGTCAAA
ACGGTATCTTCTTTAGAGGAAGCGTTGGCCTTTATTGAAAAAACTTCTTCGGGTCACACGGAAGCGATTGTCACGGAAGA
TTTAAACACGGCCAGAATTTTTACAAACTCTTTGGATTCAGCGGCTTTATTTATTAATTGTTCCACTCGATTTCATGACG
GTGGAGAATTTGGGTTAGGAGCCGAAGTAGGAATATCCACTGGAAAATTACACGTTCGTGGTCCGATGGGTTTAGTTCAT
CTTACTACCACAACAACGTATGTCACCGGGAATGGACAGATCCGGGGTTGA

Upstream 100 bases:

>100_bases
TAGGAACATTAGTTGCTCCTTCCGGAAACCGCGTTTTTTCTGAAGAGGACGTAAAAGAAATCATCCGTAAAAATAGAAAC
GGAAACGGAGGAAATAGTTT

Downstream 100 bases:

>100_bases
TTTTTGAATTCTTCCATCCTTACTGGAATTTTTGGAGGAAGTTTTGATCCTCCTCACGAAGGACATTCAGAAATTTTAAA
GTCTTTTTTTTTGGAAGTTC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKEIEYVQDLCSRAKKASKVLKQLSSSKKNKILLSLADLLEKRKAEILLANELDLKDGKEKKLSSALMDRLLLNEKRIFS
MASAVREIAALPDPIGEVTRGITLPNGLELVTRRVPLGVVMVIYESRPNVTIDVGALSFKSGNACILRGGSEAFYSNEIL
IKLFHEILIKEEIDIGSVVFVDKTDRSFMIPFFQQTSLIDIVVPRGGEGLIRFVSENSKIPVVKHDKGVCNLYIDQDADP
EKVIPIVINSKVQRPGVCNSTENLILHNGYPFRKELLEALAKEGVELLLDPSSLALYPKGKPVKQQDYLEEFLDLRLSVK
TVSSLEEALAFIEKTSSGHTEAIVTEDLNTARIFTNSLDSAALFINCSTRFHDGGEFGLGAEVGISTGKLHVRGPMGLVH
LTTTTTYVTGNGQIRG

Sequences:

>Translated_416_residues
MKEIEYVQDLCSRAKKASKVLKQLSSSKKNKILLSLADLLEKRKAEILLANELDLKDGKEKKLSSALMDRLLLNEKRIFS
MASAVREIAALPDPIGEVTRGITLPNGLELVTRRVPLGVVMVIYESRPNVTIDVGALSFKSGNACILRGGSEAFYSNEIL
IKLFHEILIKEEIDIGSVVFVDKTDRSFMIPFFQQTSLIDIVVPRGGEGLIRFVSENSKIPVVKHDKGVCNLYIDQDADP
EKVIPIVINSKVQRPGVCNSTENLILHNGYPFRKELLEALAKEGVELLLDPSSLALYPKGKPVKQQDYLEEFLDLRLSVK
TVSSLEEALAFIEKTSSGHTEAIVTEDLNTARIFTNSLDSAALFINCSTRFHDGGEFGLGAEVGISTGKLHVRGPMGLVH
LTTTTTYVTGNGQIRG
>Mature_416_residues
MKEIEYVQDLCSRAKKASKVLKQLSSSKKNKILLSLADLLEKRKAEILLANELDLKDGKEKKLSSALMDRLLLNEKRIFS
MASAVREIAALPDPIGEVTRGITLPNGLELVTRRVPLGVVMVIYESRPNVTIDVGALSFKSGNACILRGGSEAFYSNEIL
IKLFHEILIKEEIDIGSVVFVDKTDRSFMIPFFQQTSLIDIVVPRGGEGLIRFVSENSKIPVVKHDKGVCNLYIDQDADP
EKVIPIVINSKVQRPGVCNSTENLILHNGYPFRKELLEALAKEGVELLLDPSSLALYPKGKPVKQQDYLEEFLDLRLSVK
TVSSLEEALAFIEKTSSGHTEAIVTEDLNTARIFTNSLDSAALFINCSTRFHDGGEFGLGAEVGISTGKLHVRGPMGLVH
LTTTTTYVTGNGQIRG

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=410, Percent_Identity=37.8048780487805, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI21361368, Length=410, Percent_Identity=37.8048780487805, Blast_Score=264, Evalue=1e-70,
Organism=Escherichia coli, GI1786438, Length=409, Percent_Identity=42.7872860635697, Blast_Score=327, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI17569969, Length=413, Percent_Identity=37.2881355932203, Blast_Score=260, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=413, Percent_Identity=37.2881355932203, Blast_Score=260, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324899, Length=429, Percent_Identity=36.5967365967366, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI21357643, Length=412, Percent_Identity=36.1650485436893, Blast_Score=241, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_LEPIC (Q72NQ9)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002690.1
- ProteinModelPortal:   Q72NQ9
- SMR:   Q72NQ9
- GeneID:   2770404
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12771
- NMPDR:   fig|267671.1.peg.2690
- HOGENOM:   HBG318080
- OMA:   HRIEAMA
- ProtClustDB:   PRK00197
- BioCyc:   LINT267671:LIC_12771-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45796; Mature: 45796

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKEIEYVQDLCSRAKKASKVLKQLSSSKKNKILLSLADLLEKRKAEILLANELDLKDGKE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCCHH
KKLSSALMDRLLLNEKRIFSMASAVREIAALPDPIGEVTRGITLPNGLELVTRRVPLGVV
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHCCCEEE
MVIYESRPNVTIDVGALSFKSGNACILRGGSEAFYSNEILIKLFHEILIKEEIDIGSVVF
EEEEECCCCEEEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
VDKTDRSFMIPFFQQTSLIDIVVPRGGEGLIRFVSENSKIPVVKHDKGVCNLYIDQDADP
EECCCCEEEEEEECCCCEEEEEECCCCCCEEEEECCCCCCCEEECCCCEEEEEECCCCCC
EKVIPIVINSKVQRPGVCNSTENLILHNGYPFRKELLEALAKEGVELLLDPSSLALYPKG
CCEEEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCCEEEECCC
KPVKQQDYLEEFLDLRLSVKTVSSLEEALAFIEKTSSGHTEAIVTEDLNTARIFTNSLDS
CCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCC
AALFINCSTRFHDGGEFGLGAEVGISTGKLHVRGPMGLVHLTTTTTYVTGNGQIRG
EEEEEEECCEECCCCCCCCCCEECCCCCEEEEECCCEEEEEEEEEEEEECCCEECC
>Mature Secondary Structure
MKEIEYVQDLCSRAKKASKVLKQLSSSKKNKILLSLADLLEKRKAEILLANELDLKDGKE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCCHH
KKLSSALMDRLLLNEKRIFSMASAVREIAALPDPIGEVTRGITLPNGLELVTRRVPLGVV
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHCCCEEE
MVIYESRPNVTIDVGALSFKSGNACILRGGSEAFYSNEILIKLFHEILIKEEIDIGSVVF
EEEEECCCCEEEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
VDKTDRSFMIPFFQQTSLIDIVVPRGGEGLIRFVSENSKIPVVKHDKGVCNLYIDQDADP
EECCCCEEEEEEECCCCEEEEEECCCCCCEEEEECCCCCCCEEECCCCEEEEEECCCCCC
EKVIPIVINSKVQRPGVCNSTENLILHNGYPFRKELLEALAKEGVELLLDPSSLALYPKG
CCEEEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCCEEEECCC
KPVKQQDYLEEFLDLRLSVKTVSSLEEALAFIEKTSSGHTEAIVTEDLNTARIFTNSLDS
CCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCC
AALFINCSTRFHDGGEFGLGAEVGISTGKLHVRGPMGLVHLTTTTTYVTGNGQIRG
EEEEEEECCEECCCCCCCCCCEECCCCCEEEEECCCEEEEEEEEEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA