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The map label for this gene is nuoB

Identifier: 45658576

GI number: 45658576

Start: 3338918

End: 3339478

Strand: Direct

Name: nuoB

Synonym: LIC12742

Alternate gene names: 45658576

Gene position: 3338918-3339478 (Clockwise)

Preceding gene: 45658575

Following gene: 45658577

Centisome position: 78.06

GC content: 47.06

Gene sequence:

>561_bases
ATGGGACTAAGTGATTTGAGTAAAACCCCGGGCCAGGCACTGGGGGATATGGTTCAACTTGGAAACGTGGAATCCGTAAT
CCAATGGGGAAGAAGCTATTCCCTTTGGCCTTATCCTTTCGCTACCGCCTGTTGTGGTATCGAGTATATGAGCACCGCCT
GCTCCGATTATGACATCGCACGTTTTGGTGCGGAACGACCTTCATTTTCACCTCGGCAAGCGGATATGATTCTTGTTTTG
GGAACCATCACCTACAAGATGGCTCCGGTTTTAAGACAGATCTACGATCAAATGGCAGAACCTAAGTTTGTAATCAGTGT
AGGCGCCTGCGCTTCTTCCGGAGGAATGTTTCACACTTACGGCGTGTTACAGGGAGTAGACCGGATTCTACCCGTGGATG
TATACGTTCCAGGATGTCCTCCCAGACCCGAAGCAATTTTAGACGCACTTTTAAAATTACAAACTAAATTAAAAACCCAG
GGCTTGGAAGCAAGACGTCAAGAAGTAATGCAAAAGATCCAGGAACTCAACGAAAGAAATAAGCCCCTGGTTGTGCGATG
A

Upstream 100 bases:

>100_bases
AGAACGCTGGTCTTGGTTTTTTTCTATTCTTCGAGATGTTTGTGTTTGTTCTAACCTTAGTGGTAGGTTTATACTATATC
TGGAAGAAAGGAGCGCTGGA

Downstream 100 bases:

>100_bases
AAGAAAAAGTAGAAAGTTTTCTCAAAGAAAAATTCCCTCATTTTTTAGATTTTCAGGAAGAAGTAATATCCAACGTTCCC
GTTTTTTTTCTCAAAGTGGA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MGLSDLSKTPGQALGDMVQLGNVESVIQWGRSYSLWPYPFATACCGIEYMSTACSDYDIARFGAERPSFSPRQADMILVL
GTITYKMAPVLRQIYDQMAEPKFVISVGACASSGGMFHTYGVLQGVDRILPVDVYVPGCPPRPEAILDALLKLQTKLKTQ
GLEARRQEVMQKIQELNERNKPLVVR

Sequences:

>Translated_186_residues
MGLSDLSKTPGQALGDMVQLGNVESVIQWGRSYSLWPYPFATACCGIEYMSTACSDYDIARFGAERPSFSPRQADMILVL
GTITYKMAPVLRQIYDQMAEPKFVISVGACASSGGMFHTYGVLQGVDRILPVDVYVPGCPPRPEAILDALLKLQTKLKTQ
GLEARRQEVMQKIQELNERNKPLVVR
>Mature_185_residues
GLSDLSKTPGQALGDMVQLGNVESVIQWGRSYSLWPYPFATACCGIEYMSTACSDYDIARFGAERPSFSPRQADMILVLG
TITYKMAPVLRQIYDQMAEPKFVISVGACASSGGMFHTYGVLQGVDRILPVDVYVPGCPPRPEAILDALLKLQTKLKTQG
LEARRQEVMQKIQELNERNKPLVVR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=142, Percent_Identity=49.2957746478873, Blast_Score=159, Evalue=1e-39,
Organism=Escherichia coli, GI1788624, Length=146, Percent_Identity=52.7397260273973, Blast_Score=177, Evalue=5e-46,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=44.7154471544715, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788834, Length=120, Percent_Identity=41.6666666666667, Blast_Score=83, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=51.4084507042254, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=52.1428571428571, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=52.1428571428571, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24651058, Length=137, Percent_Identity=52.5547445255474, Blast_Score=158, Evalue=2e-39,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20578; Mature: 20447

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSDLSKTPGQALGDMVQLGNVESVIQWGRSYSLWPYPFATACCGIEYMSTACSDYDIA
CCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
RFGAERPSFSPRQADMILVLGTITYKMAPVLRQIYDQMAEPKFVISVGACASSGGMFHTY
HHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
GVLQGVDRILPVDVYVPGCPPRPEAILDALLKLQTKLKTQGLEARRQEVMQKIQELNERN
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
KPLVVR
CCEECC
>Mature Secondary Structure 
GLSDLSKTPGQALGDMVQLGNVESVIQWGRSYSLWPYPFATACCGIEYMSTACSDYDIA
CCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
RFGAERPSFSPRQADMILVLGTITYKMAPVLRQIYDQMAEPKFVISVGACASSGGMFHTY
HHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
GVLQGVDRILPVDVYVPGCPPRPEAILDALLKLQTKLKTQGLEARRQEVMQKIQELNERN
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
KPLVVR
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA