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The map label for this gene is crt [H]

Identifier: 45658559

GI number: 45658559

Start: 3316849

End: 3317649

Strand: Direct

Name: crt [H]

Synonym: LIC12725

Alternate gene names: 45658559

Gene position: 3316849-3317649 (Clockwise)

Preceding gene: 45658558

Following gene: 45658561

Centisome position: 77.55

GC content: 35.46

Gene sequence:

>801_bases
ATGGGATTCATAGACCGAGACACAATCGATCTGGGTTCAGGAAACAAAATCCTGACTTTAAGCTTCAACAATCCTGAAAC
CAGAAATTCTATGACTCGCGAAATGGGTTTAGAATTCAAAAAAATTATCGAAGGTTTGCTAGAAACTCCAGAACAAAATA
AACCAAGGGTTGTAATTTTAACGGGAAAAAACGATATTTTTTCAGCAGGCGGTAATTTCGAACTATTAAAGTCTTTTTCG
ACTAAAGACTATGAAACGAACAAAAAGACAATGTTCGAATTTTACAATCTATTCCTATCCGTAAGACGTTTGGATATTCC
AGTAATTTGTGCTGCAAACGGCCATGCAATTGGAGCGGGATTTTCACTTACATTCGCATGCGATATTCGTATTTTTGCCA
ATGAAGCCAAATATCAATTCAACTTTGTTAAACTCGGAATTCATCCTGGTATGGGTTCAAGTTATATCGTAAAAGAATTG
TTCGGAACTCATATTGCAAATCGACTTTTATTTTTAGCAGAAACATTAAATGGTGAAGAAGCGTTCCGACTTGGACTCTG
TAACGATTCAGTTCCTCAAAAAGAAGTCCTAGGAAGAGCTACTGAAATTGCAATTGCATTATCTGAAAGTGCCCCTTTAG
CGCTTAGTGAATTAAAAAAGAACACTTACGATAAGGAAAAATTAGAAGCCGCTCTTAGAAAAGAAGCTGAGTCCCAGGCA
AGAAATTTTATTTCTTCTGATTTCAAAGAAACAATTAAAGCGATCGAACAAAAAAGAAAACCAATTTTCAAAGGACTATA
A

Upstream 100 bases:

>100_bases
TTCTCGAATATTATACTCAGATGGGAATTGGAGAAAAACCGTTGACCATATCCGACCGAATCTGGTATGAAAGTTTCGAA
TTGGAGAAAAAAGCAGTTTA

Downstream 100 bases:

>100_bases
AGCATAGTAATTCCGACATTACACCTCATTTTAAAATTTATGCTCGAAATTTTTGAATAATTTCAATTCCGAACTTTGTT
TTTAAATTAAAAGAATACAG

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MGFIDRDTIDLGSGNKILTLSFNNPETRNSMTREMGLEFKKIIEGLLETPEQNKPRVVILTGKNDIFSAGGNFELLKSFS
TKDYETNKKTMFEFYNLFLSVRRLDIPVICAANGHAIGAGFSLTFACDIRIFANEAKYQFNFVKLGIHPGMGSSYIVKEL
FGTHIANRLLFLAETLNGEEAFRLGLCNDSVPQKEVLGRATEIAIALSESAPLALSELKKNTYDKEKLEAALRKEAESQA
RNFISSDFKETIKAIEQKRKPIFKGL

Sequences:

>Translated_266_residues
MGFIDRDTIDLGSGNKILTLSFNNPETRNSMTREMGLEFKKIIEGLLETPEQNKPRVVILTGKNDIFSAGGNFELLKSFS
TKDYETNKKTMFEFYNLFLSVRRLDIPVICAANGHAIGAGFSLTFACDIRIFANEAKYQFNFVKLGIHPGMGSSYIVKEL
FGTHIANRLLFLAETLNGEEAFRLGLCNDSVPQKEVLGRATEIAIALSESAPLALSELKKNTYDKEKLEAALRKEAESQA
RNFISSDFKETIKAIEQKRKPIFKGL
>Mature_265_residues
GFIDRDTIDLGSGNKILTLSFNNPETRNSMTREMGLEFKKIIEGLLETPEQNKPRVVILTGKNDIFSAGGNFELLKSFST
KDYETNKKTMFEFYNLFLSVRRLDIPVICAANGHAIGAGFSLTFACDIRIFANEAKYQFNFVKLGIHPGMGSSYIVKELF
GTHIANRLLFLAETLNGEEAFRLGLCNDSVPQKEVLGRATEIAIALSESAPLALSELKKNTYDKEKLEAALRKEAESQAR
NFISSDFKETIKAIEQKRKPIFKGL

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=26.0700389105058, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI70995211, Length=215, Percent_Identity=26.9767441860465, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI213417737, Length=239, Percent_Identity=28.0334728033473, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI157694516, Length=239, Percent_Identity=28.0334728033473, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI221136756, Length=224, Percent_Identity=27.6785714285714, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136753, Length=224, Percent_Identity=27.6785714285714, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=224, Percent_Identity=27.6785714285714, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI116235487, Length=229, Percent_Identity=24.8908296943231, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=26.953125, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694520, Length=200, Percent_Identity=26, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=26.4822134387352, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=30.1587301587302, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788597, Length=256, Percent_Identity=25.78125, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788682, Length=193, Percent_Identity=27.4611398963731, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082183, Length=273, Percent_Identity=23.0769230769231, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=242, Percent_Identity=31.8181818181818, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=24.4094488188976, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=272, Percent_Identity=25, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=23.3463035019455, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=225, Percent_Identity=25.3333333333333, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=266, Percent_Identity=23.6842105263158, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=29.7709923664122, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=25.4901960784314, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=25.4901960784314, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19922422, Length=263, Percent_Identity=24.7148288973384, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654903, Length=169, Percent_Identity=30.1775147928994, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920382, Length=264, Percent_Identity=24.6212121212121, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21357171, Length=251, Percent_Identity=24.3027888446215, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921018, Length=189, Percent_Identity=26.984126984127, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 29765; Mature: 29633

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFIDRDTIDLGSGNKILTLSFNNPETRNSMTREMGLEFKKIIEGLLETPEQNKPRVVIL
CCCCCCCCEECCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEE
TGKNDIFSAGGNFELLKSFSTKDYETNKKTMFEFYNLFLSVRRLDIPVICAANGHAIGAG
ECCCCEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECC
FSLTFACDIRIFANEAKYQFNFVKLGIHPGMGSSYIVKELFGTHIANRLLFLAETLNGEE
EEEEEEEEEEEEECCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AFRLGLCNDSVPQKEVLGRATEIAIALSESAPLALSELKKNTYDKEKLEAALRKEAESQA
EEEEECCCCCCCHHHHHCCHHEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RNFISSDFKETIKAIEQKRKPIFKGL
HHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
GFIDRDTIDLGSGNKILTLSFNNPETRNSMTREMGLEFKKIIEGLLETPEQNKPRVVIL
CCCCCCCEECCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEE
TGKNDIFSAGGNFELLKSFSTKDYETNKKTMFEFYNLFLSVRRLDIPVICAANGHAIGAG
ECCCCEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECC
FSLTFACDIRIFANEAKYQFNFVKLGIHPGMGSSYIVKELFGTHIANRLLFLAETLNGEE
EEEEEEEEEEEEECCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AFRLGLCNDSVPQKEVLGRATEIAIALSESAPLALSELKKNTYDKEKLEAALRKEAESQA
EEEEECCCCCCCHHHHHCCHHEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RNFISSDFKETIKAIEQKRKPIFKGL
HHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]