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The map label for this gene is alkA

Identifier: 45658208

GI number: 45658208

Start: 2862671

End: 2863312

Strand: Direct

Name: alkA

Synonym: LIC12362

Alternate gene names: 45658208

Gene position: 2862671-2863312 (Clockwise)

Preceding gene: 45658205

Following gene: 45658210

Centisome position: 66.93

GC content: 36.14

Gene sequence:

>642_bases
TTGAAAGATAAAATCTTAAAATTCGAAAAGAAAGAATTCTATTCCATCTGTGATCAACTTTCTCGAAAAGATCGGGGATT
ACATTCAATTCTTCTAAAGCACGGTTATCCTCCTTTTTGGAGTCGCAAACCGAACTTTGAAACCTTAGTTCATATCATCT
TAGAACAACAAGTTTCTCTTGCATCCGCAAGGGCAGCCTTAGTAAAACTGAAAAATAAGATTGGCTCCGTAACCGCCCGA
AAGATTCTTTTGTTAAGCGACATCGAATTAAGAGAATGTTATTTCAGTAGACAAAAAACCAGTTATGTTCGAGACCTTGC
AGAGTTCGTATTTTCCAAACGAATCATACTTGGCGATTTAGCCTCTAAATCAGACCAAATGATTCGAGAAGATTTGATAA
CCGTAAAAGGGATTGGAAATTGGACCGTAGATATTTTTCTGATTATGGCTTTGCACCGTGCAGATATTTTTCCACTCGGA
GATTTGGCGGCAGTTAAATCCCTAAAAAAAATTAAAAAATTACCTGTAGATACTTCTAATGATAAAATCCTGTCTGTTTC
TAAATCTTGGAGACCATTCCGCTCGATCGCCACTATGTTGCTCTGGCATTCCTACATTCAAGAAAATAATATTAAATTTT
GA

Upstream 100 bases:

>100_bases
AAACGAAATACAAATCTGTTAAAATTTTTATCTACCTCATTTAGATCGCCTTGTAATACCAAAGACAAATCTCATCTTAC
GTCCTAGAGAAGAATTAAAT

Downstream 100 bases:

>100_bases
TCCTACTTTCCCCAAGAAGATAAAAATTTTTTTAAATCGTTCATTCCAGATTGGTTATATCCCTCAGTTTTAAAAAAAAT
TTCACGTTTCGAATTTAAAA

Product: 3-methyladenine DNA glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKDKILKFEKKEFYSICDQLSRKDRGLHSILLKHGYPPFWSRKPNFETLVHIILEQQVSLASARAALVKLKNKIGSVTAR
KILLLSDIELRECYFSRQKTSYVRDLAEFVFSKRIILGDLASKSDQMIREDLITVKGIGNWTVDIFLIMALHRADIFPLG
DLAAVKSLKKIKKLPVDTSNDKILSVSKSWRPFRSIATMLLWHSYIQENNIKF

Sequences:

>Translated_213_residues
MKDKILKFEKKEFYSICDQLSRKDRGLHSILLKHGYPPFWSRKPNFETLVHIILEQQVSLASARAALVKLKNKIGSVTAR
KILLLSDIELRECYFSRQKTSYVRDLAEFVFSKRIILGDLASKSDQMIREDLITVKGIGNWTVDIFLIMALHRADIFPLG
DLAAVKSLKKIKKLPVDTSNDKILSVSKSWRPFRSIATMLLWHSYIQENNIKF
>Mature_213_residues
MKDKILKFEKKEFYSICDQLSRKDRGLHSILLKHGYPPFWSRKPNFETLVHIILEQQVSLASARAALVKLKNKIGSVTAR
KILLLSDIELRECYFSRQKTSYVRDLAEFVFSKRIILGDLASKSDQMIREDLITVKGIGNWTVDIFLIMALHRADIFPLG
DLAAVKSLKKIKKLPVDTSNDKILSVSKSWRPFRSIATMLLWHSYIQENNIKF

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 24639; Mature: 24639

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKILKFEKKEFYSICDQLSRKDRGLHSILLKHGYPPFWSRKPNFETLVHIILEQQVSL
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
ASARAALVKLKNKIGSVTARKILLLSDIELRECYFSRQKTSYVRDLAEFVFSKRIILGDL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKSDQMIREDLITVKGIGNWTVDIFLIMALHRADIFPLGDLAAVKSLKKIKKLPVDTSN
HCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
DKILSVSKSWRPFRSIATMLLWHSYIQENNIKF
CCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKDKILKFEKKEFYSICDQLSRKDRGLHSILLKHGYPPFWSRKPNFETLVHIILEQQVSL
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
ASARAALVKLKNKIGSVTARKILLLSDIELRECYFSRQKTSYVRDLAEFVFSKRIILGDL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKSDQMIREDLITVKGIGNWTVDIFLIMALHRADIFPLGDLAAVKSLKKIKKLPVDTSN
HCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
DKILSVSKSWRPFRSIATMLLWHSYIQENNIKF
CCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]