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The map label for this gene is rffG [C]

Identifier: 45658051

GI number: 45658051

Start: 2655542

End: 2656531

Strand: Reverse

Name: rffG [C]

Synonym: LIC12202

Alternate gene names: 45658051

Gene position: 2656531-2655542 (Counterclockwise)

Preceding gene: 45658052

Following gene: 45658050

Centisome position: 62.11

GC content: 37.47

Gene sequence:

>990_bases
ATGAAAGCGTTAGTTACAGGCGGAGCAGGGTTTATTGGAAGTCATCTTGTAGATCTTCTATTGGAAAATCAATTTGAGGT
TACCGTTCTGGATAATTTCAGTACGGGAAGGGCGTTTAATTTAAATCACGTAAAAGAGAAAATTGATTTAGTTGAATGTG
ATCTATCGATTCAAGAAGATTGGATCAAAAAGTTTCAATCAGTTGATTATGTATTTCATCTTGCGGCGCTCGCGGACATT
GTTCCTAGTATTCAAAACCCTGAAGGTTATTTTCAATCAAATGTAACTGGTACTCTGAATGTTCTTCAAGCTAGTAGACA
TTACGGAGTGAAGCGGTTTGTTTATGCTGCTTCTTCTTCTTGTTATGGAATTCCGGAGCTTTATCCAACTCCTGAGACTT
CGCCAATTCTACCTCAATACCCATATGCACTTACAAAAAGAATGGGGGAAGAGTTAGTCATGCACTGGGTACAAGTTTAT
AAATTTCCCGCTCTATCATTAAGGTTTTTTAATGTTTACGGACCTAGATCTAGAACTTCTGGAACTTATGGTGCCGTATT
TGGAGTTTTTTTAGCTCAAAAGTTGGCAGGGAAACCTTTCACTGTAGTTGGGGATGGAAAACAAACTAGAGATTTTACTT
ATGTGCAAGACGTTGCGGAAGCGGTTTTTGCTGCTGCCCAAAGTGATAAAGTAGGTGAAATTTATAATGTTGGAAGTGGT
GCAACGATTTCAGTCAATCGAATTGTAGAACTTTTGAAAGGAGAAGTTACTTATATTCCAAAACGACCGGGTGAACCAGA
TAGCACTTTTGCCGATATTGCAAAAATTAAAAAAGATTTAAAATGGTCCCCTAAGATTTCTATAGAAACAGGGATTGGCG
AATTATTAAAAAATATAGATTATTGGAGAGAAGCTCCAGTATGGACGCCTGATAAGATAGAAAAAGCGACATCCGATTGG
TTTAAGTATCTTGGCGGATCTAATTCATAA

Upstream 100 bases:

>100_bases
AATTCACTTTCCGATCAATGATATGCAAATCTCTGAAGATTTACAAGTAATTGTAGGCCATATGTGTATGCAATGGTTGT
GTGGAGCAAAGTAAAATAAA

Downstream 100 bases:

>100_bases
AAACGAAATTAACGTTTTAATTCTGGCGGCTGGGCTTGGAACTAGGCTAAAACCTCTTACGGATTTTTGGCCGAAATGTT
TGATGCCGATTTCCGGGAAA

Product: UDP-glucose 4-epimerase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKALVTGGAGFIGSHLVDLLLENQFEVTVLDNFSTGRAFNLNHVKEKIDLVECDLSIQEDWIKKFQSVDYVFHLAALADI
VPSIQNPEGYFQSNVTGTLNVLQASRHYGVKRFVYAASSSCYGIPELYPTPETSPILPQYPYALTKRMGEELVMHWVQVY
KFPALSLRFFNVYGPRSRTSGTYGAVFGVFLAQKLAGKPFTVVGDGKQTRDFTYVQDVAEAVFAAAQSDKVGEIYNVGSG
ATISVNRIVELLKGEVTYIPKRPGEPDSTFADIAKIKKDLKWSPKISIETGIGELLKNIDYWREAPVWTPDKIEKATSDW
FKYLGGSNS

Sequences:

>Translated_329_residues
MKALVTGGAGFIGSHLVDLLLENQFEVTVLDNFSTGRAFNLNHVKEKIDLVECDLSIQEDWIKKFQSVDYVFHLAALADI
VPSIQNPEGYFQSNVTGTLNVLQASRHYGVKRFVYAASSSCYGIPELYPTPETSPILPQYPYALTKRMGEELVMHWVQVY
KFPALSLRFFNVYGPRSRTSGTYGAVFGVFLAQKLAGKPFTVVGDGKQTRDFTYVQDVAEAVFAAAQSDKVGEIYNVGSG
ATISVNRIVELLKGEVTYIPKRPGEPDSTFADIAKIKKDLKWSPKISIETGIGELLKNIDYWREAPVWTPDKIEKATSDW
FKYLGGSNS
>Mature_329_residues
MKALVTGGAGFIGSHLVDLLLENQFEVTVLDNFSTGRAFNLNHVKEKIDLVECDLSIQEDWIKKFQSVDYVFHLAALADI
VPSIQNPEGYFQSNVTGTLNVLQASRHYGVKRFVYAASSSCYGIPELYPTPETSPILPQYPYALTKRMGEELVMHWVQVY
KFPALSLRFFNVYGPRSRTSGTYGAVFGVFLAQKLAGKPFTVVGDGKQTRDFTYVQDVAEAVFAAAQSDKVGEIYNVGSG
ATISVNRIVELLKGEVTYIPKRPGEPDSTFADIAKIKKDLKWSPKISIETGIGELLKNIDYWREAPVWTPDKIEKATSDW
FKYLGGSNS

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=316, Percent_Identity=33.5443037974684, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI7657641, Length=324, Percent_Identity=29.320987654321, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI56237023, Length=328, Percent_Identity=30.1829268292683, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI56118217, Length=328, Percent_Identity=30.1829268292683, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI189083684, Length=328, Percent_Identity=30.1829268292683, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI4504031, Length=349, Percent_Identity=26.647564469914, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI193211614, Length=232, Percent_Identity=27.1551724137931, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI8393516, Length=232, Percent_Identity=27.1551724137931, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=30.5232558139535, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788353, Length=364, Percent_Identity=28.5714285714286, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786974, Length=345, Percent_Identity=28.9855072463768, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1788366, Length=348, Percent_Identity=24.7126436781609, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788589, Length=336, Percent_Identity=26.1904761904762, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790049, Length=319, Percent_Identity=28.5266457680251, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=320, Percent_Identity=33.4375, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=31.044776119403, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI71982035, Length=348, Percent_Identity=30.1724137931034, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71982038, Length=350, Percent_Identity=30, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI115532424, Length=316, Percent_Identity=26.5822784810127, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI133901786, Length=334, Percent_Identity=25.1497005988024, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=334, Percent_Identity=25.748502994012, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539422, Length=334, Percent_Identity=25.1497005988024, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI133901788, Length=334, Percent_Identity=25.1497005988024, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI133901790, Length=334, Percent_Identity=25.1497005988024, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539424, Length=334, Percent_Identity=25.1497005988024, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71987463, Length=283, Percent_Identity=28.2685512367491, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=336, Percent_Identity=29.4642857142857, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321437, Length=352, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21356223, Length=333, Percent_Identity=32.7327327327327, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI19923002, Length=342, Percent_Identity=30.9941520467836, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24158427, Length=351, Percent_Identity=25.3561253561254, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 36607; Mature: 36607

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALVTGGAGFIGSHLVDLLLENQFEVTVLDNFSTGRAFNLNHVKEKIDLVECDLSIQED
CCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCEECHHHHHHHHHHHCCCCCHHHH
WIKKFQSVDYVFHLAALADIVPSIQNPEGYFQSNVTGTLNVLQASRHYGVKRFVYAASSS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHEECCCC
CYGIPELYPTPETSPILPQYPYALTKRMGEELVMHWVQVYKFPALSLRFFNVYGPRSRTS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
GTYGAVFGVFLAQKLAGKPFTVVGDGKQTRDFTYVQDVAEAVFAAAQSDKVGEIYNVGSG
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCC
ATISVNRIVELLKGEVTYIPKRPGEPDSTFADIAKIKKDLKWSPKISIETGIGELLKNID
CEEEHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
YWREAPVWTPDKIEKATSDWFKYLGGSNS
HHHCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKALVTGGAGFIGSHLVDLLLENQFEVTVLDNFSTGRAFNLNHVKEKIDLVECDLSIQED
CCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCEECHHHHHHHHHHHCCCCCHHHH
WIKKFQSVDYVFHLAALADIVPSIQNPEGYFQSNVTGTLNVLQASRHYGVKRFVYAASSS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHEECCCC
CYGIPELYPTPETSPILPQYPYALTKRMGEELVMHWVQVYKFPALSLRFFNVYGPRSRTS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
GTYGAVFGVFLAQKLAGKPFTVVGDGKQTRDFTYVQDVAEAVFAAAQSDKVGEIYNVGSG
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCC
ATISVNRIVELLKGEVTYIPKRPGEPDSTFADIAKIKKDLKWSPKISIETGIGELLKNID
CEEEHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
YWREAPVWTPDKIEKATSDWFKYLGGSNS
HHHCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]