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The map label for this gene is rfbB

Identifier: 45657972

GI number: 45657972

Start: 2566588

End: 2567637

Strand: Reverse

Name: rfbB

Synonym: LIC12124

Alternate gene names: 45657972

Gene position: 2567637-2566588 (Counterclockwise)

Preceding gene: 45657973

Following gene: 45657971

Centisome position: 60.03

GC content: 36.95

Gene sequence:

>1050_bases
ATGAAAAAAATTTTAGTTACGGGTGGAGCCGGTTTTATAGGTTCCAATTTTGTAAATCTCATCTTAAACGATACCAAAGA
ATATCAGGTAGTCGTATTCGATAAGCTGACTTATGCTGGAAATCTGAGAAGCTTGGAATCTTGGAAAAAAGATTCTCGAT
TTATTTTTGTAAAGGCAGATATCGCCAATAAAGAAGAGGTATCTTCGATCTTTCAAGAGCATAAATTTAATTACATTGCA
CACTTTGCGGCAGAAAGCCACGTAGATCGTTCTATTTCAGGACCGGAAGAATTCATCAAGACGAATGTGCTTGGTACTTT
TTATCTTTTGGATGCTGCTAGACTACAATGGAATGGATCTTATGAAGGAAAAAAATTTCTTCATGTATCTACGGACGAAG
TATTTGGAACGTTAGGAGATAGCGGATATTTCACAGAGGAAACTCCTTATGCACCTAATTCTCCTTATTCGGCCTCTAAA
GCCAGTTCGGATCATATCGTAAGATCTTACTACCATACTTATCATATGCCGGTAGTGACTACAAATTGTTCCAATAATTA
TGGACCTTATCATTTTCCCGAAAAGTTGATTCCTTTGATGATTTTAAATTGTTTACAAGGAAAACCTCTACCCGTTTACG
GAGACGGAAAGAATATTAGAGATTGGTTATATGTTAAGGATCATTGTGAGGCTCTACGTGTTGCTTTGTTCCAGGGATTG
CCCGGGGAAACTTACAACATAGGAACTAGAAACGAAAAAAAGAATATAGACATAGTAGATTCCATTTGTTCCATTATGGA
CGAGTTGCATCCTTCGGGGGCTCCACATTCTAAATTGATTCAGTATGTAAAGGACAGACCAGGTCACGATTTTCGTTATG
CAATTGATCCTTCCAAGATCGAAAAAGAATTGGGATGGAAGCCTAAATTTGCATTTGAATCGGCTTTAAAAGAAACTGTT
CGGTGGTATTTAGAGAATGAATCTTGGTGGAAGGAAATTCTTTCCGGTCAATATAAAGAATATTATGAAAATCAGTACGA
GAAACGTTAG

Upstream 100 bases:

>100_bases
ATTTAGAAGAAACTCGAAAAATTTTTGGCGTTGTTCCGCATTGGAGAGAGGATTTAACCCTTTGTCTGAAAGAACTTGCC
GAAGTTTCCGGAAAAAAAGT

Downstream 100 bases:

>100_bases
ATGAAATCTAGAAAAGGAATTATACTCGCAGGCGGTTCTGGAACAAGACTTTATCCGGTCACATACGTAGTTTCCAAACA
ACTTTTACCGGTTTATGATA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKKILVTGGAGFIGSNFVNLILNDTKEYQVVVFDKLTYAGNLRSLESWKKDSRFIFVKADIANKEEVSSIFQEHKFNYIA
HFAAESHVDRSISGPEEFIKTNVLGTFYLLDAARLQWNGSYEGKKFLHVSTDEVFGTLGDSGYFTEETPYAPNSPYSASK
ASSDHIVRSYYHTYHMPVVTTNCSNNYGPYHFPEKLIPLMILNCLQGKPLPVYGDGKNIRDWLYVKDHCEALRVALFQGL
PGETYNIGTRNEKKNIDIVDSICSIMDELHPSGAPHSKLIQYVKDRPGHDFRYAIDPSKIEKELGWKPKFAFESALKETV
RWYLENESWWKEILSGQYKEYYENQYEKR

Sequences:

>Translated_349_residues
MKKILVTGGAGFIGSNFVNLILNDTKEYQVVVFDKLTYAGNLRSLESWKKDSRFIFVKADIANKEEVSSIFQEHKFNYIA
HFAAESHVDRSISGPEEFIKTNVLGTFYLLDAARLQWNGSYEGKKFLHVSTDEVFGTLGDSGYFTEETPYAPNSPYSASK
ASSDHIVRSYYHTYHMPVVTTNCSNNYGPYHFPEKLIPLMILNCLQGKPLPVYGDGKNIRDWLYVKDHCEALRVALFQGL
PGETYNIGTRNEKKNIDIVDSICSIMDELHPSGAPHSKLIQYVKDRPGHDFRYAIDPSKIEKELGWKPKFAFESALKETV
RWYLENESWWKEILSGQYKEYYENQYEKR
>Mature_349_residues
MKKILVTGGAGFIGSNFVNLILNDTKEYQVVVFDKLTYAGNLRSLESWKKDSRFIFVKADIANKEEVSSIFQEHKFNYIA
HFAAESHVDRSISGPEEFIKTNVLGTFYLLDAARLQWNGSYEGKKFLHVSTDEVFGTLGDSGYFTEETPYAPNSPYSASK
ASSDHIVRSYYHTYHMPVVTTNCSNNYGPYHFPEKLIPLMILNCLQGKPLPVYGDGKNIRDWLYVKDHCEALRVALFQGL
PGETYNIGTRNEKKNIDIVDSICSIMDELHPSGAPHSKLIQYVKDRPGHDFRYAIDPSKIEKELGWKPKFAFESALKETV
RWYLENESWWKEILSGQYKEYYENQYEKR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=37.6506024096386, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI42516563, Length=328, Percent_Identity=26.8292682926829, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI56237023, Length=360, Percent_Identity=24.4444444444444, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56118217, Length=360, Percent_Identity=24.4444444444444, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI189083684, Length=360, Percent_Identity=24.4444444444444, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4504031, Length=347, Percent_Identity=25.9365994236311, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=59.1954022988506, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=54.7222222222222, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1786974, Length=345, Percent_Identity=26.0869565217391, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788366, Length=356, Percent_Identity=25, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=331, Percent_Identity=37.4622356495468, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=32.02416918429, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=24.773413897281, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=358, Percent_Identity=26.536312849162, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=360, Percent_Identity=26.3888888888889, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI133901788, Length=245, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=245, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=245, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=245, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=245, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=362, Percent_Identity=26.7955801104972, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356223, Length=328, Percent_Identity=27.4390243902439, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=28.9693593314763, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24158427, Length=349, Percent_Identity=24.6418338108883, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40190; Mature: 40190

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILVTGGAGFIGSNFVNLILNDTKEYQVVVFDKLTYAGNLRSLESWKKDSRFIFVKAD
CCEEEEECCCCCHHHHHHHEEECCCCCEEEEEEEEEECCCCCHHHHHHCCCCEEEEEEEC
IANKEEVSSIFQEHKFNYIAHFAAESHVDRSISGPEEFIKTNVLGTFYLLDAARLQWNGS
CCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCC
YEGKKFLHVSTDEVFGTLGDSGYFTEETPYAPNSPYSASKASSDHIVRSYYHTYHMPVVT
CCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCEEE
TNCSNNYGPYHFPEKLIPLMILNCLQGKPLPVYGDGKNIRDWLYVKDHCEALRVALFQGL
ECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHEEEHHHHHHHHHHHHHCC
PGETYNIGTRNEKKNIDIVDSICSIMDELHPSGAPHSKLIQYVKDRPGHDFRYAIDPSKI
CCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECHHHH
EKELGWKPKFAFESALKETVRWYLENESWWKEILSGQYKEYYENQYEKR
HHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKILVTGGAGFIGSNFVNLILNDTKEYQVVVFDKLTYAGNLRSLESWKKDSRFIFVKAD
CCEEEEECCCCCHHHHHHHEEECCCCCEEEEEEEEEECCCCCHHHHHHCCCCEEEEEEEC
IANKEEVSSIFQEHKFNYIAHFAAESHVDRSISGPEEFIKTNVLGTFYLLDAARLQWNGS
CCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCC
YEGKKFLHVSTDEVFGTLGDSGYFTEETPYAPNSPYSASKASSDHIVRSYYHTYHMPVVT
CCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCEEE
TNCSNNYGPYHFPEKLIPLMILNCLQGKPLPVYGDGKNIRDWLYVKDHCEALRVALFQGL
ECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHEEEHHHHHHHHHHHHHCC
PGETYNIGTRNEKKNIDIVDSICSIMDELHPSGAPHSKLIQYVKDRPGHDFRYAIDPSKI
CCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECHHHH
EKELGWKPKFAFESALKETVRWYLENESWWKEILSGQYKEYYENQYEKR
HHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]