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The map label for this gene is rfbA

Identifier: 45657971

GI number: 45657971

Start: 2565703

End: 2566587

Strand: Reverse

Name: rfbA

Synonym: LIC12123

Alternate gene names: 45657971

Gene position: 2566587-2565703 (Counterclockwise)

Preceding gene: 45657972

Following gene: 45657970

Centisome position: 60.01

GC content: 37.18

Gene sequence:

>885_bases
ATGAAATCTAGAAAAGGAATTATACTCGCAGGCGGTTCTGGAACAAGACTTTATCCGGTCACATACGTAGTTTCCAAACA
ACTTTTACCGGTTTATGATAAACCGATGATCTATTACCCACTAACAACGCTTATGCTAGCGGGTATCAAAGAAATACTTT
TGATTTCCACACCTCAAGCTACTCCTATGTATAAAGAACTGTTAGGAGATGGAAAACAATGGGGAATTTCCATAGAATAT
GCGGTACAACCCAATCCGGGAGGATTGGCGCAAGCGTATTGGATCGGAGAAAATTTCGTCAACGGACATCCGTCTGTTTT
GATTCTTGGAGATAATATCTATTTCGGTCATAATCTTGCTTCTCTTTTAGAAAACGCTTCTAAAAAAGAGAACGGTTCTA
CTGTGTTCGCGTATCCGGTTCATGATCCAGAAAGGTATGGAGTGGTAGAGTTTGATTCTGAAAGACGTGCGGTTTCGATC
GAAGAAAAACCTTCTAAACCGAAATCTAATTATGCAGTCACTGGATTATATTTTTATGATGAAGAGGTTGTTAATATAGC
TAAATCTATTAAACCTTCTGCAAGGGGAGAATTGGAGATTACAGACGTAAATAGGATTTATTTAGAAAGAGGAATTTTAA
ACGTTCAAGTGATGGGACGAGGGTATGCTTGGTTGGACACTGGGACTCACGAATCACTTTTAGAGGCATCTGTATTTATA
GAAACGATTGAAAAAAGACAAGGACTTAAAGTTGCCTGCCCGGAAGAAATTGCGTTTAGAAAAGGTTTTATAGATGGATC
TCAATTAGAAAAATTGATAAGTCCTCTTAAAAAAACAGGTTATGGAGAATATCTTGTAAAAGTACTTAATGAAAAGATTT
ACTAA

Upstream 100 bases:

>100_bases
AGAAACTGTTCGGTGGTATTTAGAGAATGAATCTTGGTGGAAGGAAATTCTTTCCGGTCAATATAAAGAATATTATGAAA
ATCAGTACGAGAAACGTTAG

Downstream 100 bases:

>100_bases
ATTTCATAGAGGCTTTACATTTTTAAATTGTTCGTTTCTTAATTTTACTATAAGCATATTATTTTCAGATTGTTATTGGA
TCGTGTAATTAGTGTTACCT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKSRKGIILAGGSGTRLYPVTYVVSKQLLPVYDKPMIYYPLTTLMLAGIKEILLISTPQATPMYKELLGDGKQWGISIEY
AVQPNPGGLAQAYWIGENFVNGHPSVLILGDNIYFGHNLASLLENASKKENGSTVFAYPVHDPERYGVVEFDSERRAVSI
EEKPSKPKSNYAVTGLYFYDEEVVNIAKSIKPSARGELEITDVNRIYLERGILNVQVMGRGYAWLDTGTHESLLEASVFI
ETIEKRQGLKVACPEEIAFRKGFIDGSQLEKLISPLKKTGYGEYLVKVLNEKIY

Sequences:

>Translated_294_residues
MKSRKGIILAGGSGTRLYPVTYVVSKQLLPVYDKPMIYYPLTTLMLAGIKEILLISTPQATPMYKELLGDGKQWGISIEY
AVQPNPGGLAQAYWIGENFVNGHPSVLILGDNIYFGHNLASLLENASKKENGSTVFAYPVHDPERYGVVEFDSERRAVSI
EEKPSKPKSNYAVTGLYFYDEEVVNIAKSIKPSARGELEITDVNRIYLERGILNVQVMGRGYAWLDTGTHESLLEASVFI
ETIEKRQGLKVACPEEIAFRKGFIDGSQLEKLISPLKKTGYGEYLVKVLNEKIY
>Mature_294_residues
MKSRKGIILAGGSGTRLYPVTYVVSKQLLPVYDKPMIYYPLTTLMLAGIKEILLISTPQATPMYKELLGDGKQWGISIEY
AVQPNPGGLAQAYWIGENFVNGHPSVLILGDNIYFGHNLASLLENASKKENGSTVFAYPVHDPERYGVVEFDSERRAVSI
EEKPSKPKSNYAVTGLYFYDEEVVNIAKSIKPSARGELEITDVNRIYLERGILNVQVMGRGYAWLDTGTHESLLEASVFI
ETIEKRQGLKVACPEEIAFRKGFIDGSQLEKLISPLKKTGYGEYLVKVLNEKIY

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761619, Length=267, Percent_Identity=22.0973782771536, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI11761621, Length=267, Percent_Identity=22.0973782771536, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788351, Length=291, Percent_Identity=70.7903780068729, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1790224, Length=288, Percent_Identity=62.1527777777778, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI133931050, Length=182, Percent_Identity=24.1758241758242, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320148, Length=252, Percent_Identity=26.984126984127, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355443, Length=251, Percent_Identity=23.5059760956175, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24644084, Length=251, Percent_Identity=23.5059760956175, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32808; Mature: 32808

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRKGIILAGGSGTRLYPVTYVVSKQLLPVYDKPMIYYPLTTLMLAGIKEILLISTPQA
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHCHHHEEEEECCCC
TPMYKELLGDGKQWGISIEYAVQPNPGGLAQAYWIGENFVNGHPSVLILGDNIYFGHNLA
CHHHHHHHCCCCHHCEEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEECCEEECCHHH
SLLENASKKENGSTVFAYPVHDPERYGVVEFDSERRAVSIEEKPSKPKSNYAVTGLYFYD
HHHHHCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEEC
EEVVNIAKSIKPSARGELEITDVNRIYLERGILNVQVMGRGYAWLDTGTHESLLEASVFI
HHHHHHHHHCCCCCCCCEEEEECCEEEHHCCEEEEEEECCCEEEEECCCHHHHHHHHHHH
ETIEKRQGLKVACPEEIAFRKGFIDGSQLEKLISPLKKTGYGEYLVKVLNEKIY
HHHHHHCCCEEECCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKSRKGIILAGGSGTRLYPVTYVVSKQLLPVYDKPMIYYPLTTLMLAGIKEILLISTPQA
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHCHHHEEEEECCCC
TPMYKELLGDGKQWGISIEYAVQPNPGGLAQAYWIGENFVNGHPSVLILGDNIYFGHNLA
CHHHHHHHCCCCHHCEEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEECCEEECCHHH
SLLENASKKENGSTVFAYPVHDPERYGVVEFDSERRAVSIEEKPSKPKSNYAVTGLYFYD
HHHHHCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEEC
EEVVNIAKSIKPSARGELEITDVNRIYLERGILNVQVMGRGYAWLDTGTHESLLEASVFI
HHHHHHHHHCCCCCCCCEEEEECCEEEHHCCEEEEEEECCCEEEEECCCHHHHHHHHHHH
ETIEKRQGLKVACPEEIAFRKGFIDGSQLEKLISPLKKTGYGEYLVKVLNEKIY
HHHHHHCCCEEECCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]